LOCUS       CaTCH_Dual_sgRNA        8544 bp    DNA        circular     26-MAY-2020
DEFINITION  .
ACCESSION   urn.local...d-37vq1om
VERSION     urn.local...d-37vq1om
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    118..298
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    350..487
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="psi"
                     /label="psi"
                     /ApEinfo_label="psi"
                     /ApEinfo_fwdcolor="#f9ad81"
                     /ApEinfo_revcolor="#f9ad81"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    409..453
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack"
                     /label="HIV-1_psi_pack"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    454..818
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="gag"
                     /label="gag"
                     /ApEinfo_label="gag"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    964..1205
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1612..1851
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="hU6_promoter"
                     /label="hU6_promoter"
                     /ApEinfo_label="hU6_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 2379..2470
                     /created_by="User"
                     /locus_tag="improved tracer"
                     /label="improved tracer"
                     /ApEinfo_label="improved tracer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2513..2630
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT"
                     /label="cPPT"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#fdc68a"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2674..2924
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="EFIas; EFS; EFS"
                     /label="EFIas; EFS; EFS"
                     /ApEinfo_label="EFIas; EFS; EFS"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 1872..1963
                     /created_by="User"
                     /locus_tag="improved tracer(1)"
                     /label="improved tracer(1)"
                     /ApEinfo_label="improved tracer"
                     /ApEinfo_fwdcolor="#fd902b"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             2930..3415
                     /created_by="User"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="99.79%"
                     /locus_tag="Thy1.1"
                     /label="Thy1.1"
                     /ApEinfo_label="Thy1.1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3133..3133
                     /Original_Bases="T"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="T"
                     /label="T"
                     /ApEinfo_label="T"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3253..3253
                     /Original_Bases="C"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="C"
                     /label="C"
                     /ApEinfo_label="C"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3310..3310
                     /Original_Bases="G"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="G"
                     /label="G"
                     /ApEinfo_label="G"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3416..3481
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#f6989d"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3485..4273
                     /gene="NeoR/KanR"
                     /X-Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer - NeoR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer - NeoR, pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="NeoR/KanR"
                     /label="NeoR/KanR"
                     /ApEinfo_label="NeoR/KanR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    4287..4875
                     /Transferred_From="pLentiCRISPR"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4930..4951
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT"
                     /label="U3PPT"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4946..5181
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="3' LTR SIN"
                     /label="3' LTR SIN"
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4947..4999
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta_U3"
                     /label="delta_U3"
                     /ApEinfo_label="delta_U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5000..5180
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR; truncHIV-1_3_LTR(1)"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR(1)"
                     /ApEinfo_label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polya_signal    5258..5388
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 polyadenylation signal"
                     /label="SV40 polyadenylation signal"
                     /ApEinfo_label="SV40 polyadenylation signal"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5261..5380
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_PA_terminator"
                     /label="SV40_PA_terminator"
                     /ApEinfo_label="SV40_PA_terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5398..5496
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_origin"
                     /label="SV40_origin"
                     /ApEinfo_label="SV40_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5468..5543
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 origin of replication"
                     /label="SV40 origin of replication"
                     /ApEinfo_label="SV40 origin of replication"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5575..5593)
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="T7_promoter"
                     /label="T7_promoter"
                     /ApEinfo_label="T7_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5612..5760
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="lacZ_a"
                     /label="lacZ_a"
                     /ApEinfo_label="lacZ_a"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5761..6216
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6277..6305
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; TIGPB; TIGPB-Cas9(PX330); TIGPN"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="AmpR_promoter"
                     /label="AmpR_promoter"
                     /ApEinfo_label="AmpR_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            6347..7207
                     /gene="Ampicillin"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; TIGPB; TIGPB-Cas9(PX330); TIGPN"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="99.88%"
                     /locus_tag="Ampicillin"
                     /label="Ampicillin"
                     /ApEinfo_label="Ampicillin"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7355..8022
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8290..8319
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="lac_promoter"
                     /label="lac_promoter"
                     /ApEinfo_label="lac_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8389..8408
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="T3_promoter"
                     /label="T3_promoter"
                     /ApEinfo_label="T3_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             join(8431..8544,1..299)
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR"
                     /label="5'LTR"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2044..2356
                     /locus_tag="mouse-U6"
                     /label="mouse-U6"
                     /ApEinfo_label="mouse-U6"
                     /ApEinfo_fwdcolor="#fffca0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2076..2076
                     /locus_tag="SNP A instead of G - clone-1"
                     /label="SNP A instead of G - clone-1"
                     /ApEinfo_label="SNP A instead of G - clone-1"
                     /ApEinfo_fwdcolor="#ff0003"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4930..4945
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT(1)"
                     /label="cPPT(1)"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2359..2378
                     /locus_tag="sgBC1-C"
                     /label="sgBC1-C"
                     /ApEinfo_label="sgBC1-C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1852..1871
                     /locus_tag="sgBC1-A"
                     /label="sgBC1-A"
                     /ApEinfo_label="sgBC1-A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
       61 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      241 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      301 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      361 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      421 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      481 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      541 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      601 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      661 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      721 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      781 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      841 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      901 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      961 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1021 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1081 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1141 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1201 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1261 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1321 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1381 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1441 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1501 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1561 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
     1621 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     1681 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     1741 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     1801 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cGCTGTGCTC
     1861 GTTCCACACA Cgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
     1921 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     1981 gaactttgtc tcacctgctg ctgggaccaa gctaattata aagccacccc gaggggaccc
     2041 agagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
     2101 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     2161 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     2221 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
     2281 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
     2341 tggagaaaag ccttgtttGG GCGAGCGCGC GCAAATGGgt ttaagagcta tgctggaaac
     2401 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     2461 gtgctttttt tgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     2521 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2581 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2641 acagggacag cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg
     2701 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2761 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2821 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2881 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac gcggccacca tgaacccagc
     2941 catcagcgtc gctctcctgc tctcagtctt gcaggtgtcc cgagggcaga aggtgaccag
     3001 cctgacagcc tgcctggtga accaaaacct tcgcctggac tgccgccatg agaataacac
     3061 caaggataac tccatccagc atgagttcag cctgacccga gagaagagga agcacgtgct
     3121 ctcaggcacc ctcgggatac ccgagcacac gtaccgctcc cgcgtcaccc tctccaacca
     3181 gccctatatc aaggtcctta ccctagccaa cttcaccacc aaggatgagg gcgactactt
     3241 ttgtgagctt cgagtctcgg gcgcgaatcc catgagctcc aataaaagta tcagtgtgta
     3301 tagagacaaa ctggtcaagt gtggcggcat aagcctgctg gttcagaaca catcctggat
     3361 gctgctgctg ctgctttccc tctccctcct ccaagccctg gacttcattt ctctgggcag
     3421 cggcgccacc aacttcagcc tgctgaagca ggccggcgac gtggaggaga accccggccc
     3481 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3541 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3601 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3661 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3721 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3781 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3841 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3901 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3961 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4021 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4081 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4141 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4201 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4261 tgacgagttc ttctgattaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4321 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4381 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4441 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4501 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4561 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4621 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4681 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4741 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4801 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4861 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     4921 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaacaaga
     4981 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5041 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5101 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5161 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     5221 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5521 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     5581 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5641 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5701 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     5761 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5821 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5881 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5941 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6001 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6061 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6121 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6181 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6361 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6421 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6481 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6541 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6601 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6661 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6721 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6781 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6841 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6901 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6961 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7021 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7081 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7141 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7201 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7501 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8041 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8101 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8161 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8221 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8281 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8341 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8401 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     8461 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8521 gcatgccgat tggtggaagt aagg   
//
