LOCUS       CaTCH_Dual_sgRNA        8543 bp    DNA        circular     26-MAY-2020
DEFINITION  .
ACCESSION   urn.local...d-37vq1om
VERSION     urn.local...d-37vq1om
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    118..298
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    350..487
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="psi"
                     /label="psi"
                     /ApEinfo_label="psi"
                     /ApEinfo_fwdcolor="#f9ad81"
                     /ApEinfo_revcolor="#f9ad81"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    409..453
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_psi_pack"
                     /label="HIV-1_psi_pack"
                     /ApEinfo_label="HIV-1_psi_pack"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    454..818
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="gag"
                     /label="gag"
                     /ApEinfo_label="gag"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    964..1205
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="RRE"
                     /label="RRE"
                     /ApEinfo_label="RRE"
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1612..1851
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="hU6_promoter"
                     /label="hU6_promoter"
                     /ApEinfo_label="hU6_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 2378..2469
                     /created_by="User"
                     /locus_tag="improved tracer"
                     /label="improved tracer"
                     /ApEinfo_label="improved tracer"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2512..2629
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT"
                     /label="cPPT"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#fdc68a"
                     /ApEinfo_revcolor="#fdc68a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2673..2923
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="EFIas; EFS; EFS"
                     /label="EFIas; EFS; EFS"
                     /ApEinfo_label="EFIas; EFS; EFS"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_difference 1872..1963
                     /created_by="User"
                     /locus_tag="improved tracer(1)"
                     /label="improved tracer(1)"
                     /ApEinfo_label="improved tracer"
                     /ApEinfo_fwdcolor="#fd902b"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     cds             2929..3414
                     /created_by="User"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="99.79%"
                     /locus_tag="Thy1.1"
                     /label="Thy1.1"
                     /ApEinfo_label="Thy1.1"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3132..3132
                     /Original_Bases="T"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="T"
                     /label="T"
                     /ApEinfo_label="T"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3252..3252
                     /Original_Bases="C"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="C"
                     /label="C"
                     /ApEinfo_label="C"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3309..3309
                     /Original_Bases="G"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="G"
                     /label="G"
                     /ApEinfo_label="G"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3415..3480
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#f6989d"
                     /ApEinfo_revcolor="#f6989d"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3484..4272
                     /gene="NeoR/KanR"
                     /X-Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer - NeoR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiCrispr mCherryP2AneoR-improved
                     tracer - NeoR, pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="NeoR/KanR"
                     /label="NeoR/KanR"
                     /ApEinfo_label="NeoR/KanR"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    4286..4874
                     /Transferred_From="pLentiCRISPR"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="WPRE"
                     /label="WPRE"
                     /ApEinfo_label="WPRE"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4929..4950
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="U3PPT"
                     /label="U3PPT"
                     /ApEinfo_label="U3PPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             4945..5180
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="3' LTR SIN"
                     /label="3' LTR SIN"
                     /ApEinfo_label="3' LTR SIN"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4946..4998
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="delta_U3"
                     /label="delta_U3"
                     /ApEinfo_label="delta_U3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4999..5179
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HIV-1_5_LTR; truncHIV-1_3_LTR(1)"
                     /label="HIV-1_5_LTR; truncHIV-1_3_LTR(1)"
                     /ApEinfo_label="HIV-1_5_LTR; truncHIV-1_3_LTR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polya_signal    5257..5387
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 polyadenylation signal"
                     /label="SV40 polyadenylation signal"
                     /ApEinfo_label="SV40 polyadenylation signal"
                     /ApEinfo_fwdcolor="#6ecff6"
                     /ApEinfo_revcolor="#6ecff6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      5260..5379
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_PA_terminator"
                     /label="SV40_PA_terminator"
                     /ApEinfo_label="SV40_PA_terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5397..5495
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40_origin"
                     /label="SV40_origin"
                     /ApEinfo_label="SV40_origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5467..5542
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="SV40 origin of replication"
                     /label="SV40 origin of replication"
                     /ApEinfo_label="SV40 origin of replication"
                     /ApEinfo_fwdcolor="#8882be"
                     /ApEinfo_revcolor="#8882be"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5574..5592)
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="T7_promoter"
                     /label="T7_promoter"
                     /ApEinfo_label="T7_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5611..5759
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="lacZ_a"
                     /label="lacZ_a"
                     /ApEinfo_label="lacZ_a"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5760..6215
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="F1 ori"
                     /label="F1 ori"
                     /ApEinfo_label="F1 ori"
                     /ApEinfo_fwdcolor="#f49ac2"
                     /ApEinfo_revcolor="#f49ac2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6276..6304
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; TIGPB; TIGPB-Cas9(PX330); TIGPN"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="AmpR_promoter"
                     /label="AmpR_promoter"
                     /ApEinfo_label="AmpR_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            6346..7206
                     /gene="Ampicillin"
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer; TIGPB; TIGPB-Cas9(PX330); TIGPN"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="99.88%"
                     /locus_tag="Ampicillin"
                     /label="Ampicillin"
                     /ApEinfo_label="Ampicillin"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      7354..8021
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="pUC ori"
                     /label="pUC ori"
                     /ApEinfo_label="pUC ori"
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8289..8318
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="lac_promoter"
                     /label="lac_promoter"
                     /ApEinfo_label="lac_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8388..8407
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /note="Geneious type: promoter prokaryotic"
                     /locus_tag="T3_promoter"
                     /label="T3_promoter"
                     /ApEinfo_label="T3_promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     ltr             join(8430..8543,1..299)
                     /X-Transferred_From="pLentiCRISPR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentiCRISPR,
                     pLentisgRNA-Thy1.1-P2A-Neo, pLentisgRNA-Thy1.1-P2A-Neo
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="5'LTR"
                     /label="5'LTR"
                     /ApEinfo_label="5'LTR"
                     /ApEinfo_fwdcolor="#bc8dbf"
                     /ApEinfo_revcolor="#bc8dbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2044..2356
                     /locus_tag="mouse-U6"
                     /label="mouse-U6"
                     /ApEinfo_label="mouse-U6"
                     /ApEinfo_fwdcolor="#fffca0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2076..2076
                     /locus_tag="SNP A instead of G - clone-1"
                     /label="SNP A instead of G - clone-1"
                     /ApEinfo_label="SNP A instead of G - clone-1"
                     /ApEinfo_fwdcolor="#ff0003"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4929..4944
                     /X-Transferred_From="LentiCrisprmCherryP2Apuro-improved
                     tracer"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pLentisgRNA-Thy1.1-P2A-Neo,
                     pLentisgRNA-Thy1.1-P2A-Neo Copy"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="cPPT(1)"
                     /label="cPPT(1)"
                     /ApEinfo_label="cPPT"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1852..1871
                     /locus_tag="sgBC2-A"
                     /label="sgBC2-A"
                     /ApEinfo_label="sgBC2-A"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2359..2377
                     /locus_tag="sgBC2-C"
                     /label="sgBC2-C"
                     /ApEinfo_label="sgBC2-C"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
       61 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      241 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      301 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      361 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      421 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      481 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      541 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      601 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      661 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      721 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      781 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      841 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      901 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      961 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1021 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1081 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1141 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1201 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1261 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1321 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1381 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1441 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1501 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1561 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
     1621 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     1681 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     1741 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     1801 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cGTAGGAAAC
     1861 TTCAAATACC Agtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt
     1921 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgaattc tagatcttga
     1981 gaactttgtc tcacctgctg ctgggaccaa gctaattata aagccacccc gaggggaccc
     2041 agagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag
     2101 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     2161 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     2221 tgataggctt ggatttctat aagagataca aatactaaat tattatttta aaaaacagca
     2281 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct
     2341 tggagaaaag ccttgtttGA GGTCTGGAAT ATCAGCCgtt taagagctat gctggaaaca
     2401 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     2461 tgcttttttt gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     2521 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2581 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2641 cagggacagc agagatccac tttggcgccg gctcgagtgg ctccggtgcc cgtcagtggg
     2701 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2761 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2821 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2881 ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cggccaccat gaacccagcc
     2941 atcagcgtcg ctctcctgct ctcagtcttg caggtgtccc gagggcagaa ggtgaccagc
     3001 ctgacagcct gcctggtgaa ccaaaacctt cgcctggact gccgccatga gaataacacc
     3061 aaggataact ccatccagca tgagttcagc ctgacccgag agaagaggaa gcacgtgctc
     3121 tcaggcaccc tcgggatacc cgagcacacg taccgctccc gcgtcaccct ctccaaccag
     3181 ccctatatca aggtccttac cctagccaac ttcaccacca aggatgaggg cgactacttt
     3241 tgtgagcttc gagtctcggg cgcgaatccc atgagctcca ataaaagtat cagtgtgtat
     3301 agagacaaac tggtcaagtg tggcggcata agcctgctgg ttcagaacac atcctggatg
     3361 ctgctgctgc tgctttccct ctccctcctc caagccctgg acttcatttc tctgggcagc
     3421 ggcgccacca acttcagcct gctgaagcag gccggcgacg tggaggagaa ccccggcccc
     3481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     4021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4261 gacgagttct tctgattaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     4321 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4381 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4441 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4501 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4561 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4621 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4681 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4741 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4801 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4861 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     4921 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaacaagat
     4981 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5041 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5101 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5161 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     5221 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5281 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5341 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5401 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5461 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5521 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5581 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5641 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5701 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5761 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5821 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5881 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5941 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6001 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6061 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6121 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6181 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     6241 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6301 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6361 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6421 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6481 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6541 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6601 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6661 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6721 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6781 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6841 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6901 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6961 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7021 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7081 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7141 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7201 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7261 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7501 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8041 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8101 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8161 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8221 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8281 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8341 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     8401 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     8461 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     8521 catgccgatt ggtggaagta agg   
//
