LOCUS       pBJG1_OGT_D554N         8412 bp    DNA     circular SYN 30-MAY-2020
DEFINITION  pET-24 b (+).
ACCESSION   urn.local...50-bzf7dif
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          complement(1..8412)
                     /organism="pET-24 b (+)"
                     /mol_type="other DNA"
     gene            complement(560..1375)
                     /gene="KanR2"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="KanR2"
     CDS             order(complement(560..1375),complement(560..1375))
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
                     /label="ORF frame 2"
     rep_origin      1481..2100
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="pBR322_origin"
     misc_feature    2499..2521
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="pGEX_3_primer"
     misc_feature    complement(2515..2706)
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="ROP"
     gene            complement(3181..3456)
                     /gene="tet (576 - 851)"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="tet (576 - 851)"
     CDS             order(complement(3515..4474),complement(3515..4474))
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
                     /label="ORF frame 2"
     misc_feature    complement(3515..4606)
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="lacI"
     CDS             order(complement(4347..4847),complement(4347..4847))
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="ORF frame 1"
     gene            complement(4689..4952)
                     /gene="tet (300 - 563)"
                     /label="tet (300 - 563)"
     misc_feature    4881..4900
                     /label="pBRrevBam_primer"
     promoter        4984..5002
                     /label="T7_promoter"
     misc_feature    5002..5029
                     /label="lacO"
     misc_feature    5040..5098
                     /created_by="User"
                     /label="endOfOGTvec"
     misc_feature    5047..5063
                     /label="T7_transl_en_RBS"
     misc_feature    5071..5089
                     /label="Xpress_fwd_primer"
     motif           5072..5074
                     /created_by="User"
                     /modified_by="User"
                     /label="start codon"
     misc_feature    5072..>5098
                     /label="T7_leader"
     ORF             5099..8239
                     /created_by="User"
                     /modified_by="User"
                     /label="OGT"
     misc_feature    5114..8248
                     /Original_Bases="AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG"
                     /label="AATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAG"
                     /note="Geneious type: Editing History Replacement"
     motif           5114..5137
                     /created_by="User"
                     /label="8xHis"
     motif           5138..5161
                     /created_by="User"
                     /label="HRV3C Cleavage Site"
     gene            5168..8239
                     /created_by="User"
                     /label="OGT"
     misc_difference 6803..6805
                     /created_by="User"
                     /label="D554N"
     source          complement(<8254..>8332)
                     /organism="pET-42 a(+)"
                     /mol_type="other DNA"
     CDS             <8254..8277
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
                     RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
                     VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
                     SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGR
                     GSMGYRGSEFCTGLGAPAGELRRQACGRTRAPPPPPPPLID*"
                     /label="ORF frame 3"
     misc_feature    8268..8332
                     /Original_Bases="GAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTG
                     GCTGCTGCCACCGCTGA"
                     /label="GAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCC
                     ACCGCTGA"
                     /note="Geneious type: Editing History Replacement"
     terminator      8296..>8332
                     /label="T7_terminator"
     misc_feature    complement(8329..>8332)
                     /label="T7_Terminal_primer"
     terminator      <8333..8412
                     /label="T7_terminator"
     misc_feature    complement(<8333..8344)
                     /label="T7_Terminal_primer"
ORIGIN      
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatataca tatggctagc atgactggtg gacagcaaat
     5101 gggtcgggat ccgcaccatc accatcacca tcaccacctg gaagttttgt tccaaggtcc
     5161 gggttctgct gactctaccg gtctggctga actggctcac cgtgaatacc aggctggtga
     5221 cttcgaagct gctgaacgtc actgcatgca gctgtggcgt caggaaccgg acaacaccgg
     5281 tgttctgctg ctgctgtctt ctatccactt ccagtgccgt cgtctggacc gttctgctca
     5341 cttctctacc ctggctatca aacagaaccc gctgctggct gaagcttact ctaacctggg
     5401 taacgtttac aaagaacgtg gtcagctgca ggaagctatc gaacactacc gtcacgctct
     5461 gcgtctgaaa ccggacttca tcgacggtta catcaacctg gctgctgctc tggttgctgc
     5521 tggtgacatg gaaggtgctg ttcaggctta cgtttctgct ctgcagtaca acccggacct
     5581 gtactgcgtt cgttctgacc tgggtaacct gctgaaagct ctgggtcgtc tggaagaagc
     5641 taaagcttgc tacctgaaag ctatcgaaac ccagccgaac ttcgctgttg cttggtctaa
     5701 cctgggttgc gttttcaacg ctcagggtga aatctggctg gctatccacc acttcgaaaa
     5761 agctgttacc ctggacccga acttcctgga cgcttacatc aacctgggta acgttctgaa
     5821 agaagctcgt atcttcgacc gtgctgttgc tgcttacctg cgtgctctgt ctctgtctcc
     5881 gaaccacgct gttgttcacg gtaacctggc ttgcgtttac tacgaacagg gtctgatcga
     5941 cctggctatc gacacctacc gtcgtgctat cgaactgcag ccgcactttc cagatgctta
     6001 ctgcaacctg gctaacgctc tgaaagaaaa aggttctgtt gctgaagctg aagactgcta
     6061 caacaccgct ctgcgtctgt gcccgaccca cgctgactct ctgaacaacc tggctaacat
     6121 caaacgtgaa cagggtaaca tcgaagaagc tgttcgtctg taccgtaaag ctctggaagt
     6181 tttcccggaa ttcgctgctg ctcactctaa cctggcttct gttctgcagc agcagggtaa
     6241 actgcaggaa gctctgatgc actacaaaga agctatccgt atctctccga ccttcgctga
     6301 cgcttactct aacatgggta acaccctgaa agaaatgcag gacgttcagg gtgctctgca
     6361 gtgctacacc cgtgctatcc agatcaaccc ggctttcgct gacgctcact ctaacctggc
     6421 ttctatccac aaagactctg gtaacatccc ggaagctatc gcttcttacc gtaccgctct
     6481 gaaactgaaa ccggacttcc cggacgccta ctgcaacctg gctcactgcc tgcagatcgt
     6541 ttgcgactgg accgactacg acgaacgtat gaaaaaactg gtttctatcg ttgctgacca
     6601 gctggaaaaa aaccgtctgc cgtctgttca cccgcaccac tctatgctgt acccgctgtc
     6661 tcacggtttc cgtaaagcta tcgctgaacg tcacggtaac ctgtgcctgg acaaaatcaa
     6721 cgttctgcac aaaccgccgt acgaacaccc gaaagacctg aaactgtctg acggtcgtct
     6781 gcgtgttggt tacgtttctt ctaacttcgg taaccacccg acctctcacc tgatgcagtc
     6841 tatcccgggt atgcacaacc cggacaaatt cgaagttttc tgctacgctc tgtctccgga
     6901 cgacggtacc aacttccgtg ttaaagttat ggctgaagct aaccacttca tcgacctgtc
     6961 tcagatcccg tgcaacggta aagctgctga ccgtatccac caggacggta tccacatcct
     7021 ggttaacatg aacggttaca ccaaaggtgc tcgtaacgaa ctgttcgctc tgcgtccggc
     7081 tccgattcag gctatgtggc tgggttaccc gggtacctct ggtgctctgt tcatggacta
     7141 catcatcacc gaccaggaaa cctctccggc tgaagttgct gaacagtact ctgaaaaact
     7201 ggcttacatg ccgcacacct tcttcatcgg tgaccacgct aacatgttcc cgcacctgaa
     7261 aaaaaaagct gttatcgact tcaaatctaa cggtcacatc tacgacaacc gtatcgttct
     7321 gaacggtatc gacctgaaag ctttcctgga ctctctgccg gacgttaaaa tcgttaaaat
     7381 gaaatgcccg gacggcggtg acaacgctga ctcttctaac accgctctga acatgccggt
     7441 tatcccgatg aacaccatcg ctgaagctgt tatcgaaatg atcaaccgtg gtcagatcca
     7501 gatcaccatc aacggtttct ctatctctaa cggtctggct accacccaga tcaacaacaa
     7561 agctgctacc ggtgaagaag ttccgcgtac catcatcgtt accacccgtt ctcagtacgg
     7621 tctgccggaa gacgctatcg tttactgcaa cttcaaccag ctgtacaaaa tcgacccgtc
     7681 taccctgcag atgtgggcta acatcctgaa acgtgttccg aactctgttc tgtggctgct
     7741 gcgtttcccg gctgttggtg aaccgaacat ccagcagtac gctcagaaca tgggtctgcc
     7801 gcagaaccgt atcatcttct ctccggttgc tccgaaagaa gaacacgttc gtcgtggtca
     7861 gctggctgac gtttgcctgg acaccccgct gtgcaacggt cacaccaccg gtatggacgt
     7921 tctgtgggct ggtaccccga tggttaccat gccgggtgaa accctggctt ctcgtgttgc
     7981 tgcttctcag ctgacctgcc tgggttgcct ggaactgatc gctaaaaacc gtcaggaata
     8041 cgaagacatc gctgttaaac tgggtaccga cctggaatac ctgaaaaaag ttcgtggtaa
     8101 agtttggaaa cagcgtatct cctctccgct gttcaacacc aaacagtaca ccatggaact
     8161 ggaacgtctg tacctgcaga tgtgggaaca ctacgctgct ggtaacaaac cggaccacat
     8221 gattaaaccg gttgaataac accaccacca ccaccaccac caccactaat tgattaatac
     8281 ctaggctgct aaacaaagcc cgaaaggaag ctgagttggc tgctgccacc gcgcaataac
     8341 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
     8401 ctatatccgg at
//
