LOCUS       pENTR1A_mKate2-n        6176 bp    DNA     circular SYN 30-MAY-2020
DEFINITION  pENTR1A no ccDB (w48-1).
ACCESSION   urn.local...1d-bzez5v9
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     terminator      106..149
     terminator      281..308
     primer_bind     316..339
     misc_feature    complement(358..457)
     misc_feature    complement(358..441)
     misc_feature    449..490
                     /note="Geneious type: motif"
     misc_feature    complement(468..490)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(471..490)
                     /note="Geneious type: primer_bind_reverse"
     gene            <488..>4390
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             488..4390
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     source          <488..>4390
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            <488..1316
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    488..4389
                     /note="Geneious type: Editing History Replacement"
     CDS             <491..1183
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRI
                     KAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVA
                     VARYCDLPSKLGHR"
     misc_feature    <491..508
                     /note="Geneious type: motif"
     source          <491..>1282
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <491..>1183
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <1184..>1282
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <1184..>1282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            order(<1190..>1212,<1190..>1212)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             order(<1190..>1212,<1190..>1212)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     source          <1190..>1212
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     source          order(<1190..>1212,<1190..>1212)
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          order(<1190..>1212,<1190..>1212)
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            order(<1190..>1212,<1190..>1212)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1190..>1212
     gene            order(<1217..>1240,<1217..>1240)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             order(<1217..>1240,<1217..>1240)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     source          <1217..>1240
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     source          order(<1217..>1240,<1217..>1240)
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          order(<1217..>1240,<1217..>1240)
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            order(<1217..>1240,<1217..>1240)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1217..1240
     protein_bind    1256..>1275
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     1256..1275
     misc_feature    1276..>1282
                     /note="Geneious type: ORF"
     source          <1276..>1282
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<1276..1280)
     misc_feature    complement(1283..1342)
                     /note="Geneious type: motif"
     primer_bind     1283..1300
     primer_bind     1283..1299
     misc_feature    1283..1285
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine. {ECO:0000269|PubMed:19413330,
                     ECO:0000269|PubMed:22814378, ECO:0000269|Ref.5};
                     propagated from UniProtKB/Swiss-Prot (O15294.3);
                     acetylation site"
     misc_feature    1286..1288
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    1289..1291
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     exon            <1317..1467
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    <1317..1411
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 1"
     exon            1468..1711
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1514..1615
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 2"
     misc_feature    1616..1717
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 3"
     exon            1712..1780
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1718..1819
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 4"
     exon            1781..1897
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1820..1921
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 5"
     exon            1898..1977
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    1922..2023
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 6"
     exon            1978..2173
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2024..2125
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 7"
     misc_feature    2049..2069
                     /note="Geneious type: motif"
     misc_feature    complement(2049..2072)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2126..2227
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 8"
     exon            2174..2314
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2228..2329
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 9"
     misc_difference 2243..2245
     exon            2315..2415
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2330..2431
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 10"
     misc_difference 2345..2347
     exon            2416..2569
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2432..2533
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 11"
     misc_difference 2447..2449
     misc_feature    2534..2635
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 12"
     misc_difference 2549..2551
     exon            2570..2671
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2636..2668
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 13, truncated"
     misc_difference 2651..2653
     exon            2672..2851
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2708..2758
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
     exon            2852..3010
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    2891..2910
                     /note="Geneious type: motif"
     exon            3011..3100
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3101..3226
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3227..3397
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3398..3514
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     STS             3456..4357
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /standard_name="Ogt"
                     /db_xref="UniSTS:507110"
     exon            3515..3685
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            3686..3838
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    3688..3707
                     /note="Geneious type: motif"
     exon            3839..4091
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            4092..4215
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            4216..>4390
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    4220..4279
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Required for phosphatidylinositol
                     3,4,5-triphosphate binding"
     misc_feature    complement(4367..4390)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4369..4390)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4405..4504
     gene            4627..5436
                     /gene="KanR2 (variant)"
     CDS             4627..5436
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
                     PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
                     GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     primer_bind     5008..5028
     primer_bind     5008..5027
     misc_feature    complement(5008..5027)
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      5510..6129
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccatg gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
      541 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
      601 cacccagacc atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat
      661 cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc
      721 cgacttcttt aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga
      781 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
      841 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
      901 actcggctgg gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag
      961 agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac
     1021 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag
     1081 aagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc
     1141 tgtggccaga tactgcgacc tccctagcaa actggggcac agaggcggag actacaaaga
     1201 tgatgacgac aaaggcgact acaaagacga tgacgacaag ggcatcgata gatcaacaag
     1261 tttgtacaaa aaagcaggct ttgcgtcttc cgtgggcaac gtggccgaca gcacagggtt
     1321 agctgagttg gcacatcgag aatatcaggc aggagatttt gaggcagctg agagacactg
     1381 catgcagctc tggagacaag agccagacaa tactggtgtg cttttattac tttcatctat
     1441 acacttccag tgtcgaaggc tggacagatc tgctcacttt agcactctgg caattaaaca
     1501 gaaccccctt ctggcagaag cttattcgaa tttggggaat gtgtacaagg aaagagggca
     1561 gttgcaggag gcaattgagc attatcgaca tgcattgcgt ctcaaacctg atttcatcga
     1621 tggttatatt aacctggcag ccgccttggt agcagcgggt gacatggaag gggcagtaca
     1681 agcttacgtc tctgctcttc agtacaatcc tgatttgtac tgtgttcgca gtgacctggg
     1741 gaacctgctc aaagccctgg gtcgcttgga agaagccaag gcatgttatt tgaaagcaat
     1801 tgagacgcaa ccgaactttg cagtagcttg gagtaatctt ggctgtgttt tcaatgcaca
     1861 aggggaaatt tggcttgcaa ttcatcactt tgaaaaggct gtcacccttg acccaaactt
     1921 tctggatgct tatatcaatt taggaaatgt cttgaaagag gcacgcattt ttgacagagc
     1981 tgtggcagct tatcttcgtg ccctaagttt gagtccaaat cacgcagtgg tgcacggcaa
     2041 cctggcttgt gtatactatg agcaaggcct gatagatctg gcaatagaca cctacaggcg
     2101 ggctatcgaa ctacaaccac atttccctga tgcttactgc aacctagcca atgctctcaa
     2161 agagaagggc agtgttgctg aagcagaaga ttgttataat acagctctcc gtctgtgtcc
     2221 cacccatgca gactctctga atgcactagc caatatcaaa cgagaacagg gaaacattga
     2281 agaggcagtt cgcttgtatc gtaaagcatt agaagtcttc ccagagtttg ctgctgccca
     2341 ttcagcatta gcaagtgtac tgcagcagca gggaaaactg caggaagctc tgatgcatta
     2401 taaggaggct attcgaatca gtcctacctt tgctgatgcc tactctgcaa tgggaaacac
     2461 tctaaaggag atgcaggatg ttcagggagc cttgcagtgt tatacgcgtg ccatccaaat
     2521 taatcctgca tttgcagatg cacatagcgc actggcttcc attcataagg attcagggaa
     2581 tattccagaa gccatagctt cttaccgcac ggctctgaaa cttaagcctg attttcctga
     2641 tgcttattgt gcattggctc attgcctgca gattgtctgt gattggacag actatgatga
     2701 gcgaatgaag aagttggtca gtattgtggc tgaccagtta gagaagaata ggttgccttc
     2761 tgtgcatcct catcatagta tgctatatcc tctttctcat ggcttcagga aggctattgc
     2821 tgagaggcac ggcaacctgt gcttagataa gattaatgtt cttcataaac caccatatga
     2881 acatccaaaa gacttgaagc tcagtgatgg tcggctgcgt gtaggatatg tgagttccga
     2941 ctttgggaat catcctactt ctcaccttat gcagtctatt ccaggcatgc acaatcctga
     3001 taaatttgag gtgttctgtt atgccctgag cccagacgat ggcacaaact tccgagtgaa
     3061 ggtgatggca gaagccaatc atttcattga tctttctcag attccatgca atggaaaagc
     3121 agctgatcgc atccatcagg atggaattca tatccttgta aatatgaatg gctatactaa
     3181 gggcgctcga aatgagcttt ttgctctcag gccagctcct attcaggcaa tgtggctggg
     3241 ataccctggg acgagtggtg cgcttttcat ggattatatt atcactgatc aggaaacttc
     3301 gccagctgaa gttgctgagc agtattccga gaaattggct tatatgcccc acactttttt
     3361 tattggtgat catgctaata tgttccctca cctgaagaaa aaagcagtca tcgattttaa
     3421 gtccaatggg cacatttatg acaatcggat agttctgaat ggcatcgacc tcaaagcatt
     3481 tcttgatagt ctaccagatg tgaaaattgt caagatgaag tgtcctgatg gaggagacaa
     3541 tgcagatagc agtaacacag ctcttaatat gcctgttatt cctatgaata ctattgcaga
     3601 agcagttatt gaaatgatta accgaggaca gattcaaata acaattaatg gattcagtat
     3661 tagcaatgga ctggcaacta ctcagatcaa caataaggct gcaactggag aggaggttcc
     3721 ccgtaccatt attgtaacca cccgttctca gtacgggtta ccagaagatg ccatcgtata
     3781 ctgtaacttt aatcagttgt ataaaattga cccttctact ttgcagatgt gggcaaacat
     3841 tctgaagcgt gttcccaata gtgtactctg gctgttgcgt tttccagcag taggagaacc
     3901 taatattcaa cagtatgcac aaaacatggg cctgccccag aaccgtatca ttttttcacc
     3961 tgttgctcct aaagaggaac acgtcaggag aggccagctg gctgatgtct gcttggacac
     4021 tccactctgt aatgggcaca ccacagggat ggatgtcctc tgggcaggga cccccatggt
     4081 gactatgcca ggagagactc ttgcttctcg agttgcagca tcccagctca cttgcttagg
     4141 ttgtcttgag cttattgcta aaaacagaca agaatatgaa gacatagctg tgaagctggg
     4201 aactgatcta gaatacctga agaaagttcg tggcaaagtc tggaagcaaa gaatatctag
     4261 ccctctgttc aacaccaaac aatacacaat ggaactagag cggctctatc tacagatgtg
     4321 ggagcattat gcagctggca acaaacctga ccacatgatt aagcctgttg aagtcactga
     4381 gtcagcataa ctcgagatat ctagacccag ctttcttgta caaagttggc attataagaa
     4441 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4501 tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     4561 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     4621 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     4681 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     4741 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     4801 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     4861 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     4921 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     4981 gttgatgcgc tggcagtgtc cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     5041 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     5101 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     5161 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     5221 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     5281 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     5341 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     5401 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     5461 cattattcag attgggcccc gttccactga gcgtcagacc cggtagaaaa gatcaaagga
     5521 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5581 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5641 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5701 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5761 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5821 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5881 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5941 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6001 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6061 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6121 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
