LOCUS       LR82_pLenti_PGK_Neo_hu_ncOGT       11291 bp    DNA     circular SYN 26-FEB-2017
DEFINITION  pLenti PGK Neo DEST (w531-1).
ACCESSION   urn.local...1v-bzf0rbv
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..11291
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /gene="Ampicillin"
                     /label="Ampicillin"
     CDS             132..992
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="pBR322_origin"
     promoter        2075..2104
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="HIV-1_5_LTR"
     misc_feature    2448..2628
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="truncHIV-1_3_LTR"
     misc_feature    2736..2780
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="HIV-1_psi_pack"
     CDS             3168..4376
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="RRE"
     misc_feature    4023..4038
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="cPPT"
     motif           complement(4728..4745)
                     /created_by="User"
                     /label="rev"
     misc_feature    4746..>4753
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="attR1"
     source          <4754..>7938
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<4754..4770)
                     /label="attL1"
     misc_feature    4754
                     /label="attL2"
     misc_feature    4766..7938
                     /Original_Bases="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAA
                     ATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTC
                     ACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATA
                     ATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
                     GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAA
                     CATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGG
                     ATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTT
                     TATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAA
                     GACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGC
                     AAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCT
                     ACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAA
                     GGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTT
                     TTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAA
                     ATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCC
                     GTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATG
                     AGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTA
                     TGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGA
                     AGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGA
                     CAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAA
                     CCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGA
                     AGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAAC
                     AGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGT
                     CTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCC
                     CCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGT
                     GCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTC
                     TCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACG
                     CCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTC
                     TGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTT
                     TTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /label="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTT
                     TGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCG
                     GCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGA
                     TTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAA
                     AATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAG
                     GCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGG
                     CCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT
                     TCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAG
                     CTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAA
                     CGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATA
                     TTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATT
                     GAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAA
                     ACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATAC
                     GCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGAT
                     GGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGG
                     GCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTT
                     GCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCA
                     AAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCA
                     GTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGA
                     ATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGC
                     TGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGG
                     TGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTG
                     GATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCA
                     GTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGG
                     GGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATC
                     GGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACC
                     TGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCG
                     ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAA
                     AATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     4801..4817
                     /created_by="primer3"
                     /%GC="64.7"
                     /Tm="59.3"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATGGCGTCTTCCGTGGG"
                     /Product_Size=3141
                     /annotation_group="14447722725604490000857aaf3-761a-4cb5-9
                     f4f-7e6b8107671bNM_181672 - OGT CDS"
                     /label="1 F"
     primer_bind     4801..4817
                     /created_by="primer3"
                     /%GC="64.7"
                     /Tm="59.3"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATGGCGTCTTCCGTGGG"
                     /Product_Size=3141
                     /annotation_group="14447722725616070001ad53f35-9fd9-41b0-9
                     a0f-4d59c2ea1588NM_181672 - OGT CDS"
                     /label="1 F"
     gene            <4801..>7911
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     source          <4801..>7911
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     CDS             4801..7911
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     exon            <4801..4837
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    4804..4806
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine. {ECO:0000269|PubMed:19413330,
                     ECO:0000269|PubMed:22814378, ECO:0000269|Ref.5};
                     propagated from UniProtKB/Swiss-Prot (O15294.3);
                     acetylation site"
     misc_feature    4807..4809
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    4810..4812
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     exon            <4838..4988
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    <4838..4932
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 1"
     exon            4989..5232
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5035..5136
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 2"
     misc_feature    5137..5238
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 3"
     exon            5233..5301
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5239..5340
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 4"
     exon            5302..5418
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5341..5442
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 5"
     exon            5419..5498
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5443..5544
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 6"
     exon            5499..5694
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5545..5646
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 7"
     motif           5570..5590
                     /created_by="User"
                     /label="z_p9"
     misc_feature    5647..5748
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 8"
     exon            5695..5835
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5749..5850
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 9"
     exon            5836..5936
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5851..5952
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 10"
     exon            5937..6090
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5953..6054
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 11"
     misc_feature    6055..6156
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 12"
     exon            6091..6192
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6157..6189
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 13, truncated"
     exon            6193..6372
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6229..6279
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
     exon            6373..6531
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           6412..6431
                     /created_by="User"
                     /label="z_p10"
     exon            6532..6621
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            6622..6747
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            6748..6918
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            6919..7035
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     STS             6977..7878
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /standard_name="Ogt"
                     /db_xref="UniSTS:507110"
     exon            7036..7206
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7207..7359
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           7209..7228
                     /created_by="User"
                     /label="z_p11"
     exon            7360..7612
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7613..7736
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7737..>7911
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    7741..7800
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Required for phosphatidylinositol
                     3,4,5-triphosphate binding"
     misc_feature    complement(7888..7911)
                     /created_by="primer3"
                     /%GC="41.7"
                     /Tm="59.0"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCAAC"
                     /Product_Size=3141
                     /annotation_group="14447722725616070001ad53f35-9fd9-41b0-9
                     a0f-4d59c2ea1588NM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7890..7911)
                     /created_by="primer3"
                     /%GC="40.9"
                     /Tm="57.1"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="1.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCA"
                     /Product_Size=3141
                     /annotation_group="14447722725604490000857aaf3-761a-4cb5-9
                     f4f-7e6b8107671bNM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     5'UTR           <7912..>7914
                     /created_by="User"
                     /label="gene UTR"
     misc_feature    7929..>7938
                     /label="attL2"
     misc_feature    complement(<7939..7946)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR1"
     misc_feature    7939
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR2"
     motif           7947..7965
                     /created_by="User"
                     /label="fwd"
     misc_feature    7996..8571
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="WPRE"
     CDS             8084..8617
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="ORF frame 1"
     misc_feature    complement(8660..8683)
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="MSCV_rev_primer"
     misc_feature    9057..9076
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="mPGK_F_primer"
     gene            9138..9938
                     /gene="NeoR/KanR"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="NeoR/KanR"
     CDS             9138..9941
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="ORF frame 2"
     misc_feature    10015..10036
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="U3PPT"
     misc_feature    10015..10030
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="cPPT"
     misc_feature    10085..10265
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="truncHIV-1_3_LTR"
     misc_feature    10085..10265
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="HIV-1_5_LTR"
     terminator      10340..10459
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40_PA_terminator"
     misc_feature    10428..10447
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="EBV_rev_primer"
     rep_origin      10477..10554
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40_origin"
     misc_feature    10539..10558
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40pro_F_primer"
     promoter        complement(10645..10663)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="T7_promoter"
     promoter        complement(10673..10689)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="M13_forward20_primer"
     misc_feature    10682..10825
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="lacZ_a"
     misc_feature    complement(10682..10704)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="M13_pUC_fwd_primer"
     rep_origin      10851..11157
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="f1_origin"
ORIGIN      
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
     4801 atggcgtctt ccgtgggcaa cgtggccgac agcacagggt tagctgagtt ggcacatcga
     4861 gaatatcagg caggagattt tgaggcagct gagagacact gcatgcagct ctggagacaa
     4921 gagccagaca atactggtgt gcttttatta ctttcatcta tacacttcca gtgtcgaagg
     4981 ctggacagat ctgctcactt tagcactctg gcaattaaac agaaccccct tctggcagaa
     5041 gcttattcga atttggggaa tgtgtacaag gaaagagggc agttgcagga ggcaattgag
     5101 cattatcgac atgcattgcg tctcaaacct gatttcatcg atggttatat taacctggca
     5161 gccgccttgg tagcagcggg tgacatggaa ggggcagtac aagcttacgt ctctgctctt
     5221 cagtacaatc ctgatttgta ctgtgttcgc agtgacctgg ggaacctgct caaagccctg
     5281 ggtcgcttgg aagaagccaa ggcatgttat ttgaaagcaa ttgagacgca accgaacttt
     5341 gcagtagctt ggagtaatct tggctgtgtt ttcaatgcac aaggggaaat ttggcttgca
     5401 attcatcact ttgaaaaggc tgtcaccctt gacccaaact ttctggatgc ttatatcaat
     5461 ttaggaaatg tcttgaaaga ggcacgcatt tttgacagag ctgtggcagc ttatcttcgt
     5521 gccctaagtt tgagtccaaa tcacgcagtg gtgcacggca acctggcttg tgtatactat
     5581 gagcaaggcc tgatagatct ggcaatagac acctacaggc gggctatcga actacaacca
     5641 catttccctg atgcttactg caacctagcc aatgctctca aagagaaggg cagtgttgct
     5701 gaagcagaag attgttataa tacagctctc cgtctgtgtc ccacccatgc agactctctg
     5761 aataacctag ccaatatcaa acgagaacag ggaaacattg aagaggcagt tcgcttgtat
     5821 cgtaaagcat tagaagtctt cccagagttt gctgctgccc attcaaattt agcaagtgta
     5881 ctgcagcagc agggaaaact gcaggaagct ctgatgcatt ataaggaggc tattcgaatc
     5941 agtcctacct ttgctgatgc ctactctaat atgggaaaca ctctaaagga gatgcaggat
     6001 gttcagggag ccttgcagtg ttatacgcgt gccatccaaa ttaatcctgc atttgcagat
     6061 gcacatagca atctggcttc cattcataag gattcaggga atattccaga agccatagct
     6121 tcttaccgca cggctctgaa acttaagcct gattttcctg atgcttattg taacttggct
     6181 cattgcctgc agattgtctg tgattggaca gactatgatg agcgaatgaa gaagttggtc
     6241 agtattgtgg ctgaccagtt agagaagaat aggttgcctt ctgtgcatcc tcatcatagt
     6301 atgctatatc ctctttctca tggcttcagg aaggctattg ctgagaggca cggcaacctg
     6361 tgcttagata agattaatgt tcttcataaa ccaccatatg aacatccaaa agacttgaag
     6421 ctcagtgatg gtcggctgcg tgtaggatat gtgagttccg actttgggaa tcatcctact
     6481 tctcacctta tgcagtctat tccaggcatg cacaatcctg ataaatttga ggtgttctgt
     6541 tatgccctga gcccagacga tggcacaaac ttccgagtga aggtgatggc agaagccaat
     6601 catttcattg atctttctca gattccatgc aatggaaaag cagctgatcg catccatcag
     6661 gatggaattc atatccttgt aaatatgaat ggctatacta agggcgctcg aaatgagctt
     6721 tttgctctca ggccagctcc tattcaggca atgtggctgg gataccctgg gacgagtggt
     6781 gcgcttttca tggattatat tatcactgat caggaaactt cgccagctga agttgctgag
     6841 cagtattccg agaaattggc ttatatgccc cacacttttt ttattggtga tcatgctaat
     6901 atgttccctc acctgaagaa aaaagcagtc atcgatttta agtccaatgg gcacatttat
     6961 gacaatcgga tagttctgaa tggcatcgac ctcaaagcat ttcttgatag tctaccagat
     7021 gtgaaaattg tcaagatgaa gtgtcctgat ggaggagaca atgcagatag cagtaacaca
     7081 gctcttaata tgcctgttat tcctatgaat actattgcag aagcagttat tgaaatgatt
     7141 aaccgaggac agattcaaat aacaattaat ggattcagta ttagcaatgg actggcaact
     7201 actcagatca acaataaggc tgcaactgga gaggaggttc cccgtaccat tattgtaacc
     7261 acccgttctc agtacgggtt accagaagat gccatcgtat actgtaactt taatcagttg
     7321 tataaaattg acccttctac tttgcagatg tgggcaaaca ttctgaagcg tgttcccaat
     7381 agtgtactct ggctgttgcg ttttccagca gtaggagaac ctaatattca acagtatgca
     7441 caaaacatgg gcctgcccca gaaccgtatc attttttcac ctgttgctcc taaagaggaa
     7501 cacgtcagga gaggccagct ggctgatgtc tgcttggaca ctccactctg taatgggcac
     7561 accacaggga tggatgtcct ctgggcaggg acccccatgg tgactatgcc aggagagact
     7621 cttgcttctc gagttgcagc atcccagctc acttgcttag gttgtcttga gcttattgct
     7681 aaaaacagac aagaatatga agacatagct gtgaagctgg gaactgatct agaatacctg
     7741 aagaaagttc gtggcaaagt ctggaagcaa agaatatcta gccctctgtt caacaccaaa
     7801 caatacacaa tggaactaga gcggctctat ctacagatgt gggagcatta tgcagctggc
     7861 aacaaacctg accacatgat taagcctgtt gaagtcactg agtcagcata ataactcgag
     7921 atatctagac ccagctttgt tcagctttct tgtacaaagt ggttgatatc cagcacagtg
     7981 gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     8041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     8101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     8161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     8221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     8281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     8341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     8401 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     8461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     8521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     8581 gcctggaatt ctgcagatat ccggactagt gatctaattc taccgggtag gggaggcgct
     8641 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     8701 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     8761 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     8821 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     8881 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     8941 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     9001 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     9061 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     9121 ttcgacctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
     9181 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     9241 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     9301 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     9361 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     9421 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     9481 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     9541 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     9601 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     9661 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     9721 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     9781 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     9841 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     9901 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatccg tcgagggccc
     9961 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    10021 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
    10081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct gcctagggaa
    10141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10261 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
    10321 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10441 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
    10501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10621 tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
    10681 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    10741 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    10801 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg
    10861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    10921 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    10981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    11041 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    11101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    11161 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    11221 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
    11281 ttacaatttc c
//
