LOCUS       pLentiPGKPuro_mKate2-ncOGT_5N5A       11901 bp ds-DNA     circular SYN 30-MAY-2020
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK
            promoter, Puro.
ACCESSION   . (modified)
VERSION     .
KEYWORDS    pLenti PGK Puro DEST (w529-2).
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Campeau, E, Ruhl, VE, Rodier, F, Smith, CL, Rahmberg, BL, Fuss, JO,
            Campisi, J, Yaswen, P, Cooper, PK, Kaufman and PD.
  TITLE     A versatile viral system for expression and depletion of proteins
            in mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     complement(350..369)
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     rep_origin      1163..1751
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     1652..1671
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     primer_bind     1905..1922
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     protein_bind    2039..2060
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     promoter        2075..2105
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2113..2129
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2118..2140
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     primer_bind     2137..2153
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     2137..2153
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     2172..2192
                     /note="T3 promoter, forward primer"
                     /label="T3"
     promoter        2174..2192
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     LTR             2448..2628
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    2672..2797
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    3290..3523
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to
                     thecytoplasm."
                     /label="RRE"
     misc_feature    4019..4136
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        4183..4693
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     primer_bind     4612..4630
                     /note="Human PGK promoter, forward primer"
                     /label="hPGK-F"
     source          <4746..>8754
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     misc_feature    4746..>4753
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="attR1"
     source          <4754..>8727
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<4754..4770)
                     /label="attL1"
     misc_feature    4754
                     /label="attL2"
     misc_feature    4755..4770
                     /Sequence="TACAAAAAAGCAGGCT"
                     /Hairpin_Tm="None"
                     /Tm="47.3"
                     /Self_Dimer_Tm="None"
                     /%GC="37.5"
                     /created_by="primer3"
                     /label="overlap"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    4766..8727
                     /Original_Bases="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAA
                     ATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTC
                     ACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATA
                     ATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
                     GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAA
                     CATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGG
                     ATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTT
                     TATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAA
                     GACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGC
                     AAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCT
                     ACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAA
                     GGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTT
                     TTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAA
                     ATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCC
                     GTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATG
                     AGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTA
                     TGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGA
                     AGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGA
                     CAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAA
                     CCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGA
                     AGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAAC
                     AGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGT
                     CTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCC
                     CCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGT
                     GCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTC
                     TCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACG
                     CCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTC
                     TGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTT
                     TTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /label="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTT
                     TGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCG
                     GCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGA
                     TTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAA
                     AATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAG
                     GCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGG
                     CCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT
                     TCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAG
                     CTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAA
                     CGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATA
                     TTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATT
                     GAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAA
                     ACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATAC
                     GCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGAT
                     GGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGG
                     GCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTT
                     GCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCA
                     AAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCA
                     GTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGA
                     ATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGC
                     TGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGG
                     TGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTG
                     GATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCA
                     GTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGG
                     GGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATC
                     GGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACC
                     TGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCG
                     ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAA
                     AATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /note="Geneious type: Editing History Replacement"
     source          <4771..>4800
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     gene            <4801..>8703
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             4801..8703
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     motif           <4801..4803
                     /created_by="User"
                     /modified_by="User"
                     /label="overlap"
     source          <4801..>8703
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <4801..>8703
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            <4801..5629
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     CDS             <4804..5496
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRI
                     KAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVA
                     VARYCDLPSKLGHR"
                     /label="mKate2"
     motif           <4804..4821
                     /created_by="User"
                     /label="primerFwd"
     source          <4804..>5595
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <4804..>5496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <5497..>5595
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <5497..>5595
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            order(<5503..>5525,<5503..>5525)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             order(<5503..>5525,<5503..>5525)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     source          <5503..>5525
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     source          order(<5503..>5525,<5503..>5525)
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          order(<5503..>5525,<5503..>5525)
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            order(<5503..>5525,<5503..>5525)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5503..>5525
                     /created_by="User"
                     /modified_by="User"
                     /label="FLAG"
     gene            order(<5530..>5553,<5530..>5553)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     CDS             order(<5530..>5553,<5530..>5553)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     source          <5530..>5553
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     source          order(<5530..>5553,<5530..>5553)
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          order(<5530..>5553,<5530..>5553)
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     exon            order(<5530..>5553,<5530..>5553)
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5530..5553
                     /created_by="User"
                     /modified_by="User"
                     /label="FLAG"
     protein_bind    5569..>5588
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR1"
     primer_bind     5569..5588
                     /Sequence="ACAAGTTTGTACAAAAAAGC"
                     /Hairpin_Tm="41.6"
                     /Tm="51.0"
                     /Self_Dimer_Tm="14.5"
                     /%GC="30.0"
                     /created_by="primer3"
                     /label="doesn'tChange"
     ORF             5589..>5595
                     /created_by="User"
                     /modified_by="User"
                     /label="toMove"
     source          <5589..>5595
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<5589..5593)
                     /label="attL1"
     motif           complement(5596..5655)
                     /created_by="User"
                     /label="overlap"
     primer_bind     5596..5613
                     /Sequence="GCGTCTTCCGTGGGCAAC"
                     /Hairpin_Tm="39.3"
                     /Tm="61.7"
                     /Self_Dimer_Tm="None"
                     /%GC="66.7"
                     /created_by="primer3"
                     /label="linearizeFwd"
     primer_bind     5596..5613
                     /created_by="primer3"
                     /%GC="66.7"
                     /Tm="61.7"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="5.5"
                     /Sequence="GCGTCTTCCGTGGGCAAC"
                     /Product_Size=3748
                     /annotation_group="11312239756285da7775f7-ee09-4cca-928a-4
                     68888c66ad4urn:sequence:genbank:null"
                     /label="1,283 F"
     primer_bind     5596..5612
                     /created_by="primer3"
                     /%GC="64.7"
                     /Tm="60.3"
                     /Hairpin_Tm="39.3"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="5.5"
                     /Sequence="GCGTCTTCCGTGGGCAA"
                     /Product_Size=3748
                     /annotation_group="1131223460912309fab94c-d42a-4fa0-a2f3-2
                     e06199728e1urn:sequence:genbank:null"
                     /label="1,283 F"
     misc_feature    5596..5598
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine. {ECO:0000269|PubMed:19413330,
                     ECO:0000269|PubMed:22814378, ECO:0000269|Ref.5};
                     propagated from UniProtKB/Swiss-Prot (O15294.3);
                     acetylation site"
     misc_feature    5599..5601
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    5602..5604
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     exon            <5630..5780
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    <5630..5724
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 1"
     exon            5781..6024
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5827..5928
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 2"
     misc_feature    5929..6030
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 3"
     exon            6025..6093
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6031..6132
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 4"
     exon            6094..6210
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6133..6234
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 5"
     exon            6211..6290
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6235..6336
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 6"
     exon            6291..6486
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6337..6438
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 7"
     motif           6362..6382
                     /created_by="User"
                     /label="z_p9"
     misc_feature    complement(6362..6385)
                     /Sequence="TCAGGCCTTGCTCATAGTATACAC"
                     /Hairpin_Tm="None"
                     /Tm="60.0"
                     /Self_Dimer_Tm="5.5"
                     /%GC="45.8"
                     /created_by="primer3"
                     /label="zp25"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6439..6540
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 8"
     exon            6487..6627
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6541..6642
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 9"
     misc_difference 6556..6558
                     /created_by="User"
                     /modified_by="User"
                     /label="N322A"
     exon            6628..6728
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6643..6744
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 10"
     misc_difference 6658..6660
                     /created_by="User"
                     /modified_by="User"
                     /label="N356A"
     exon            6729..6882
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6745..6846
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 11"
     misc_difference 6760..6762
                     /created_by="User"
                     /label="N390A"
     misc_feature    6847..6948
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 12"
     misc_difference 6862..6864
                     /created_by="User"
                     /label="N424A"
     exon            6883..6984
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6949..6981
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 13, truncated"
     misc_difference 6964..6966
                     /created_by="User"
                     /label="N468A"
     exon            6985..7164
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    7021..7071
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
     exon            7165..7323
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           7204..7223
                     /created_by="User"
                     /label="z_p10"
     exon            7324..7413
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7414..7539
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7540..7710
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7711..7827
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     STS             7769..8670
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /standard_name="Ogt"
                     /db_xref="UniSTS:507110"
     exon            7828..7998
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7999..8151
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           8001..8020
                     /created_by="User"
                     /label="z_p11"
     exon            8152..8404
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            8405..8528
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            8529..>8703
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    8533..8592
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Required for phosphatidylinositol
                     3,4,5-triphosphate binding"
     misc_feature    complement(8680..8703)
                     /created_by="primer3"
                     /%GC="41.7"
                     /Tm="59.0"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCAAC"
                     /Product_Size=3141
                     /annotation_group="14447722725616070001ad53f35-9fd9-41b0-9
                     a0f-4d59c2ea1588NM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(8682..8703)
                     /created_by="primer3"
                     /%GC="40.9"
                     /Tm="57.1"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="1.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCA"
                     /Product_Size=3141
                     /annotation_group="14447722725604490000857aaf3-761a-4cb5-9
                     f4f-7e6b8107671bNM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    8718..>8727
                     /label="attL2"
     misc_feature    complement(<8728..8735)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR1"
     misc_feature    8728
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR2"
     motif           8736..8754
                     /created_by="User"
                     /label="fwd"
     misc_feature    8783..9371
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     primer_bind     complement(8836..8856)
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     CDS             complement(9254..9265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     promoter        9385..9884
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     primer_bind     complement(9420..9443)
                     /note="Murine stem cell virus, reverse primer"
                     /label="MSCV-rev"
     primer_bind     9817..9836
                     /note="Mouse PGK promoter, forward primer"
                     /label="mPGK-F"
     CDS             9905..10504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
                     /label="PuroR"
     primer_bind     complement(9905..9924)
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
                     /label="Puro-R"
     primer_bind     10401..10421
                     /note="Puromycin resistance gene, forward primer"
                     /label="Puro-F"
     LTR             10642..10875
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (Delta-U3)"
     polyA_signal    10947..11068
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10984..11003)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     11038..11057
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     rep_origin      11087..11222
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     11149..11168
                     /note="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     primer_bind     complement(11254..11273)
                     /note="T7 promoter, forward primer"
                     /label="T7"
     promoter        complement(11255..11273)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(11283..11300)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /label="M13 Forward"
     primer_bind     complement(11283..11299)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 fwd"
     primer_bind     complement(11292..11314)
                     /note="In lacZ gene"
                     /label="M13/pUC Forward"
     rep_origin      11444..11899
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(11531..11550)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     11741..11762
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
ORIGIN      
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
     4801 atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     4861 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     4921 accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct
     4981 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
     5041 tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata cgaagacggg
     5101 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     5161 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     5221 tgggaggcct ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac
     5281 atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga
     5341 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg
     5401 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     5461 agatactgcg acctccctag caaactgggg cacagaggcg gagactacaa agatgatgac
     5521 gacaaaggcg actacaaaga cgatgacgac aagggcatcg atagatcaac aagtttgtac
     5581 aaaaaagcag gctttgcgtc ttccgtgggc aacgtggccg acagcacagg gttagctgag
     5641 ttggcacatc gagaatatca ggcaggagat tttgaggcag ctgagagaca ctgcatgcag
     5701 ctctggagac aagagccaga caatactggt gtgcttttat tactttcatc tatacacttc
     5761 cagtgtcgaa ggctggacag atctgctcac tttagcactc tggcaattaa acagaacccc
     5821 cttctggcag aagcttattc gaatttgggg aatgtgtaca aggaaagagg gcagttgcag
     5881 gaggcaattg agcattatcg acatgcattg cgtctcaaac ctgatttcat cgatggttat
     5941 attaacctgg cagccgcctt ggtagcagcg ggtgacatgg aaggggcagt acaagcttac
     6001 gtctctgctc ttcagtacaa tcctgatttg tactgtgttc gcagtgacct ggggaacctg
     6061 ctcaaagccc tgggtcgctt ggaagaagcc aaggcatgtt atttgaaagc aattgagacg
     6121 caaccgaact ttgcagtagc ttggagtaat cttggctgtg ttttcaatgc acaaggggaa
     6181 atttggcttg caattcatca ctttgaaaag gctgtcaccc ttgacccaaa ctttctggat
     6241 gcttatatca atttaggaaa tgtcttgaaa gaggcacgca tttttgacag agctgtggca
     6301 gcttatcttc gtgccctaag tttgagtcca aatcacgcag tggtgcacgg caacctggct
     6361 tgtgtatact atgagcaagg cctgatagat ctggcaatag acacctacag gcgggctatc
     6421 gaactacaac cacatttccc tgatgcttac tgcaacctag ccaatgctct caaagagaag
     6481 ggcagtgttg ctgaagcaga agattgttat aatacagctc tccgtctgtg tcccacccat
     6541 gcagactctc tgaatgcact agccaatatc aaacgagaac agggaaacat tgaagaggca
     6601 gttcgcttgt atcgtaaagc attagaagtc ttcccagagt ttgctgctgc ccattcagca
     6661 ttagcaagtg tactgcagca gcagggaaaa ctgcaggaag ctctgatgca ttataaggag
     6721 gctattcgaa tcagtcctac ctttgctgat gcctactctg caatgggaaa cactctaaag
     6781 gagatgcagg atgttcaggg agccttgcag tgttatacgc gtgccatcca aattaatcct
     6841 gcatttgcag atgcacatag cgcactggct tccattcata aggattcagg gaatattcca
     6901 gaagccatag cttcttaccg cacggctctg aaacttaagc ctgattttcc tgatgcttat
     6961 tgtgcattgg ctcattgcct gcagattgtc tgtgattgga cagactatga tgagcgaatg
     7021 aagaagttgg tcagtattgt ggctgaccag ttagagaaga ataggttgcc ttctgtgcat
     7081 cctcatcata gtatgctata tcctctttct catggcttca ggaaggctat tgctgagagg
     7141 cacggcaacc tgtgcttaga taagattaat gttcttcata aaccaccata tgaacatcca
     7201 aaagacttga agctcagtga tggtcggctg cgtgtaggat atgtgagttc cgactttggg
     7261 aatcatccta cttctcacct tatgcagtct attccaggca tgcacaatcc tgataaattt
     7321 gaggtgttct gttatgccct gagcccagac gatggcacaa acttccgagt gaaggtgatg
     7381 gcagaagcca atcatttcat tgatctttct cagattccat gcaatggaaa agcagctgat
     7441 cgcatccatc aggatggaat tcatatcctt gtaaatatga atggctatac taagggcgct
     7501 cgaaatgagc tttttgctct caggccagct cctattcagg caatgtggct gggataccct
     7561 gggacgagtg gtgcgctttt catggattat attatcactg atcaggaaac ttcgccagct
     7621 gaagttgctg agcagtattc cgagaaattg gcttatatgc cccacacttt ttttattggt
     7681 gatcatgcta atatgttccc tcacctgaag aaaaaagcag tcatcgattt taagtccaat
     7741 gggcacattt atgacaatcg gatagttctg aatggcatcg acctcaaagc atttcttgat
     7801 agtctaccag atgtgaaaat tgtcaagatg aagtgtcctg atggaggaga caatgcagat
     7861 agcagtaaca cagctcttaa tatgcctgtt attcctatga atactattgc agaagcagtt
     7921 attgaaatga ttaaccgagg acagattcaa ataacaatta atggattcag tattagcaat
     7981 ggactggcaa ctactcagat caacaataag gctgcaactg gagaggaggt tccccgtacc
     8041 attattgtaa ccacccgttc tcagtacggg ttaccagaag atgccatcgt atactgtaac
     8101 tttaatcagt tgtataaaat tgacccttct actttgcaga tgtgggcaaa cattctgaag
     8161 cgtgttccca atagtgtact ctggctgttg cgttttccag cagtaggaga acctaatatt
     8221 caacagtatg cacaaaacat gggcctgccc cagaaccgta tcattttttc acctgttgct
     8281 cctaaagagg aacacgtcag gagaggccag ctggctgatg tctgcttgga cactccactc
     8341 tgtaatgggc acaccacagg gatggatgtc ctctgggcag ggacccccat ggtgactatg
     8401 ccaggagaga ctcttgcttc tcgagttgca gcatcccagc tcacttgctt aggttgtctt
     8461 gagcttattg ctaaaaacag acaagaatat gaagacatag ctgtgaagct gggaactgat
     8521 ctagaatacc tgaagaaagt tcgtggcaaa gtctggaagc aaagaatatc tagccctctg
     8581 ttcaacacca aacaatacac aatggaacta gagcggctct atctacagat gtgggagcat
     8641 tatgcagctg gcaacaaacc tgaccacatg attaagcctg ttgaagtcac tgagtcagca
     8701 taactcgaga tatctagacc cagctttgtt cagctttctt gtacaaagtg gttgatatcc
     8761 agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     8821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     8881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     9001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     9061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     9121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     9181 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     9241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     9301 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     9361 cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     9421 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     9481 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     9541 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     9601 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     9661 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     9721 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     9781 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     9841 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct
     9901 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9961 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
    10021 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
    10081 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
    10141 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
    10201 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
    10261 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
    10321 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
    10381 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
    10441 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
    10501 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
    10561 tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
    10621 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
    10681 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    10741 gcctagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    10801 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    10861 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    10921 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    10981 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    11041 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    11101 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    11161 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    11221 cctaggcttt tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    11281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    11341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    11401 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc
    11461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    11581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    11641 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    11701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    11761 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    11821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    11881 atattaacgt ttacaatttc c
//
