LOCUS       LR82_pLenti_PGK_Neo_dTAG-hu_ncOGT       11717 bp    DNA     circular SYN 12-JUN-2018
DEFINITION  pLenti PGK Neo DEST (w531-1).
ACCESSION   urn.local...2b-bzf1mtl
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
            .
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="pLenti PGK Neo DEST (w531-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /gene="Ampicillin"
                     /label="Ampicillin"
     CDS             132..992
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="pBR322_origin"
     promoter        2075..2104
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="HIV-1_5_LTR"
     misc_feature    2448..2628
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="truncHIV-1_3_LTR"
     misc_feature    2736..2780
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="HIV-1_psi_pack"
     CDS             3168..4376
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="RRE"
     misc_feature    4023..4038
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="cPPT"
     motif           complement(4728..4745)
                     /created_by="User"
                     /label="rev"
     misc_feature    4746..>4753
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDHETSLEKLD
                     IEFPRGWGCAFSKAALGLRRDAAALGVVPGNAAAPTLGLAHSSRPFAASPGSSPLPLW
                     APRRRFLLRP*"
                     /label="attR1"
     source          <4754..>8364
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<4754..4770)
                     /label="attL1"
     misc_feature    4754
                     /label="attL2"
     misc_feature    4766..8364
                     /Original_Bases="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAA
                     ATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTC
                     ACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATA
                     ATGTGTGGATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAAT
                     GGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAA
                     CATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGG
                     ATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTT
                     TATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAA
                     GACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGC
                     AAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCT
                     ACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAA
                     GGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTT
                     TTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAA
                     ATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCC
                     GTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATG
                     AGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTA
                     TGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGA
                     AGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGA
                     CAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAA
                     CCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGA
                     AGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAAC
                     AGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGT
                     CTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCC
                     CCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGT
                     GCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTC
                     TCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACG
                     CCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTC
                     TGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTT
                     TTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /label="TGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTT
                     TGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCG
                     GCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGA
                     TTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAA
                     AATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAG
                     GCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGG
                     CCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT
                     TCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAG
                     CTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAA
                     CGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATA
                     TTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATT
                     GAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAA
                     ACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATAC
                     GCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGAT
                     GGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGG
                     GCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTT
                     GCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCA
                     AAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCA
                     GTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGA
                     ATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGC
                     TGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGG
                     TGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTG
                     GATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCA
                     GTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGG
                     GGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATC
                     GGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACC
                     TGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCG
                     ACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAA
                     AATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC"
                     /note="Geneious type: Editing History Replacement"
     gene            <4801..>8337
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /note="O-linked N-acetylglucosamine (GlcNAc) transferase"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
     source          <4801..>8337
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13"
     CDS             <4801..8337
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /EC_number="2.4.1.255"
                     /note="isoform 1 is encoded by transcript variant 1;
                     O-GlcNAc transferase p110 subunit;
                     uridinediphospho-N-acetylglucosamine:polypeptide
                     beta-N-acetylglucosaminyl transferase;
                     UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit; O-GlcNAc
                     transferase subunit p110; O-linked N-acetylglucosamine
                     transferase 110 kDa subunit; O-linked N-acetylglucosamine
                     (GlcNAc) transferase
                     (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl
                     transferase)"
                     /codon_start=1
                     /product="UDP-N-acetylglucosamine--peptide
                     N-acetylglucosaminyltransferase 110 kDa subunit isoform 1"
                     /protein_id="NP_858058.1"
                     /db_xref="GI:32307148"
                     /db_xref="CCDS:CCDS14414.1"
                     /db_xref="GeneID:8473"
                     /db_xref="HGNC:HGNC:8127"
                     /db_xref="HPRD:02222"
                     /db_xref="MIM:300255"
                     /translation="MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHC
                     MQLWRQEPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKER
                     GQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVR
                     SDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAV
                     TLDPNFLDAYINLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLID
                     LAIDTYRRAIELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLA
                     NIKREQGNIEEAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISP
                     TFADAYSNMGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA
                     SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPH
                     HSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFG
                     NHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKA
                     ADRIHQDGIHILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQE
                     TSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGID
                     LKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQIT
                     INGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPS
                     TLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRR
                     GQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKN
                     RQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAG
                     NKPDHMIKPVEVTESA"
     exon            <4801..5263
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     primer_bind     4801..4824
                     /Sequence="ATGGGAGTGCAGGTGGAAACCATC"
                     /Hairpin_Tm="35.3"
                     /Tm="65.0"
                     /Self_Dimer_Tm="None"
                     /%GC="54.2"
                     /created_by="primer3"
                     /label="fwd"
     source          <4801..>5229
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     source          <4801..>5229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             4801..5124
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /note="binds synthetic ligands that do not bind wild-type
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRN
                     KPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDV
                     ELLKLE"
                     /label="FKBP-V"
     CDS             5131..5157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5161..5187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /label="HA"
     protein_bind    5203..>5222
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
                     /label="attR1"
     primer_bind     5203..5222
                     /Sequence="ACAAGTTTGTACAAAAAAGC"
                     /Hairpin_Tm="41.6"
                     /Tm="51.0"
                     /Self_Dimer_Tm="14.5"
                     /%GC="30.0"
                     /created_by="primer3"
                     /label="doesn'tChange"
     ORF             5223..>5229
                     /created_by="User"
                     /modified_by="User"
                     /label="toMove"
     source          <5223..>5229
                     /organism="pENTR1A no ccDB (w48-1)"
                     /mol_type="other DNA"
     misc_feature    complement(<5223..5227)
                     /label="attL1"
     misc_feature    5230..5232
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N-acetylalanine. {ECO:0000269|PubMed:19413330,
                     ECO:0000269|PubMed:22814378, ECO:0000269|Ref.5};
                     propagated from UniProtKB/Swiss-Prot (O15294.3);
                     acetylation site"
     misc_feature    5233..5235
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     misc_feature    5236..5238
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine, by GSK3-beta, alternate.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (O15294.3); phosphorylation site"
     exon            <5264..5414
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    <5264..5358
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 1"
     exon            5415..5658
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5461..5562
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 2"
     misc_feature    5563..5664
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 3"
     exon            5659..5727
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5665..5766
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 4"
     exon            5728..5844
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5767..5868
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 5"
     exon            5845..5924
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5869..5970
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 6"
     exon            5925..6120
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    5971..6072
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 7"
     motif           5996..6016
                     /created_by="User"
                     /label="z_p9"
     misc_feature    6073..6174
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 8"
     exon            6121..6261
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6175..6276
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 9"
     exon            6262..6362
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6277..6378
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 10"
     exon            6363..6516
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6379..6480
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 11"
     misc_feature    6481..6582
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 12"
     exon            6517..6618
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6583..6615
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: TPR 13, truncated"
     exon            6619..6798
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    6655..6705
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Nuclear localization signal. {ECO:0000255}"
     exon            6799..6957
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           6838..6857
                     /created_by="User"
                     /label="z_p10"
     exon            6958..7047
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7048..7173
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7174..7344
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7345..7461
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     STS             7403..8304
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /standard_name="Ogt"
                     /db_xref="UniSTS:507110"
     exon            7462..7632
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            7633..7785
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     motif           7635..7654
                     /created_by="User"
                     /label="z_p11"
     exon            7786..8038
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            8039..8162
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     exon            8163..>8337
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /inference="alignment:Splign:1.39.8"
     misc_feature    8167..8226
                     /gene="OGT"
                     /gene_synonym="HINCUT-1; HRNT1; O-GLCNAC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (O15294.3);
                     Region: Required for phosphatidylinositol
                     3,4,5-triphosphate binding"
     misc_feature    complement(8314..8337)
                     /created_by="primer3"
                     /%GC="41.7"
                     /Tm="59.0"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCAAC"
                     /Product_Size=3141
                     /annotation_group="14447722725616070001ad53f35-9fd9-41b0-9
                     a0f-4d59c2ea1588NM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(8316..8337)
                     /created_by="primer3"
                     /%GC="40.9"
                     /Tm="57.1"
                     /Hairpin_Tm="33.4"
                     /Self_Dimer_Tm="1.4"
                     /Pair_Dimer_Tm="None"
                     /Sequence="TTATGCTGACTCAGTGACTTCA"
                     /Product_Size=3141
                     /annotation_group="14447722725604490000857aaf3-761a-4cb5-9
                     f4f-7e6b8107671bNM_181672 - OGT CDS"
                     /label="3,141 R"
                     /note="Geneious type: primer_bind_reverse"
     5'UTR           <8338..>8340
                     /created_by="User"
                     /label="gene UTR"
     misc_feature    8355..>8364
                     /label="attL2"
     misc_feature    complement(<8365..8372)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR1"
     misc_feature    8365
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="attR2"
     motif           8373..8391
                     /created_by="User"
                     /label="fwd"
     misc_feature    8422..8997
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label="WPRE"
     CDS             8510..9043
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="ORF frame 1"
     misc_feature    complement(9086..9109)
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="MSCV_rev_primer"
     misc_feature    9483..9502
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="mPGK_F_primer"
     gene            9564..10364
                     /gene="NeoR/KanR"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGILQISGLVI*"
                     /label="NeoR/KanR"
     CDS             9564..10367
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="ORF frame 2"
     misc_feature    10441..10462
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="U3PPT"
     misc_feature    10441..10456
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="cPPT"
     misc_feature    10511..10691
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="truncHIV-1_3_LTR"
     misc_feature    10511..10691
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="HIV-1_5_LTR"
     terminator      10766..10885
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40_PA_terminator"
     misc_feature    10854..10873
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="EBV_rev_primer"
     rep_origin      10903..10980
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40_origin"
     misc_feature    10965..10984
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="SV40pro_F_primer"
     promoter        complement(11071..11089)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="T7_promoter"
     promoter        complement(11099..11115)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="M13_forward20_primer"
     misc_feature    11108..11251
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="lacZ_a"
     misc_feature    complement(11108..11130)
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="M13_pUC_fwd_primer"
     rep_origin      11277..11583
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA
                     QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG
                     QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD
                     LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA
                     DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="f1_origin"
ORIGIN      
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga
     4741 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
     4801 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
     4861 cagacctgcg tggtgcacta caccgggatg cttgaagatg gaaagaaagt tgattcctcc
     4921 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
     4981 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
     5041 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
     5101 gatgtggagc ttctaaaact ggaaggcggc tacccctacg acgtgcccga ctacgccggc
     5161 tatccgtatg atgtcccgga ctatgcaggc atcgatagat caacaagttt gtacaaaaaa
     5221 gcaggctttg cgtcttccgt gggcaacgtg gccgacagca cagggttagc tgagttggca
     5281 catcgagaat atcaggcagg agattttgag gcagctgaga gacactgcat gcagctctgg
     5341 agacaagagc cagacaatac tggtgtgctt ttattacttt catctataca cttccagtgt
     5401 cgaaggctgg acagatctgc tcactttagc actctggcaa ttaaacagaa cccccttctg
     5461 gcagaagctt attcgaattt ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca
     5521 attgagcatt atcgacatgc attgcgtctc aaacctgatt tcatcgatgg ttatattaac
     5581 ctggcagccg ccttggtagc agcgggtgac atggaagggg cagtacaagc ttacgtctct
     5641 gctcttcagt acaatcctga tttgtactgt gttcgcagtg acctggggaa cctgctcaaa
     5701 gccctgggtc gcttggaaga agccaaggca tgttatttga aagcaattga gacgcaaccg
     5761 aactttgcag tagcttggag taatcttggc tgtgttttca atgcacaagg ggaaatttgg
     5821 cttgcaattc atcactttga aaaggctgtc acccttgacc caaactttct ggatgcttat
     5881 atcaatttag gaaatgtctt gaaagaggca cgcatttttg acagagctgt ggcagcttat
     5941 cttcgtgccc taagtttgag tccaaatcac gcagtggtgc acggcaacct ggcttgtgta
     6001 tactatgagc aaggcctgat agatctggca atagacacct acaggcgggc tatcgaacta
     6061 caaccacatt tccctgatgc ttactgcaac ctagccaatg ctctcaaaga gaagggcagt
     6121 gttgctgaag cagaagattg ttataataca gctctccgtc tgtgtcccac ccatgcagac
     6181 tctctgaata acctagccaa tatcaaacga gaacagggaa acattgaaga ggcagttcgc
     6241 ttgtatcgta aagcattaga agtcttccca gagtttgctg ctgcccattc aaatttagca
     6301 agtgtactgc agcagcaggg aaaactgcag gaagctctga tgcattataa ggaggctatt
     6361 cgaatcagtc ctacctttgc tgatgcctac tctaatatgg gaaacactct aaaggagatg
     6421 caggatgttc agggagcctt gcagtgttat acgcgtgcca tccaaattaa tcctgcattt
     6481 gcagatgcac atagcaatct ggcttccatt cataaggatt cagggaatat tccagaagcc
     6541 atagcttctt accgcacggc tctgaaactt aagcctgatt ttcctgatgc ttattgtaac
     6601 ttggctcatt gcctgcagat tgtctgtgat tggacagact atgatgagcg aatgaagaag
     6661 ttggtcagta ttgtggctga ccagttagag aagaataggt tgccttctgt gcatcctcat
     6721 catagtatgc tatatcctct ttctcatggc ttcaggaagg ctattgctga gaggcacggc
     6781 aacctgtgct tagataagat taatgttctt cataaaccac catatgaaca tccaaaagac
     6841 ttgaagctca gtgatggtcg gctgcgtgta ggatatgtga gttccgactt tgggaatcat
     6901 cctacttctc accttatgca gtctattcca ggcatgcaca atcctgataa atttgaggtg
     6961 ttctgttatg ccctgagccc agacgatggc acaaacttcc gagtgaaggt gatggcagaa
     7021 gccaatcatt tcattgatct ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc
     7081 catcaggatg gaattcatat ccttgtaaat atgaatggct atactaaggg cgctcgaaat
     7141 gagctttttg ctctcaggcc agctcctatt caggcaatgt ggctgggata ccctgggacg
     7201 agtggtgcgc ttttcatgga ttatattatc actgatcagg aaacttcgcc agctgaagtt
     7261 gctgagcagt attccgagaa attggcttat atgccccaca ctttttttat tggtgatcat
     7321 gctaatatgt tccctcacct gaagaaaaaa gcagtcatcg attttaagtc caatgggcac
     7381 atttatgaca atcggatagt tctgaatggc atcgacctca aagcatttct tgatagtcta
     7441 ccagatgtga aaattgtcaa gatgaagtgt cctgatggag gagacaatgc agatagcagt
     7501 aacacagctc ttaatatgcc tgttattcct atgaatacta ttgcagaagc agttattgaa
     7561 atgattaacc gaggacagat tcaaataaca attaatggat tcagtattag caatggactg
     7621 gcaactactc agatcaacaa taaggctgca actggagagg aggttccccg taccattatt
     7681 gtaaccaccc gttctcagta cgggttacca gaagatgcca tcgtatactg taactttaat
     7741 cagttgtata aaattgaccc ttctactttg cagatgtggg caaacattct gaagcgtgtt
     7801 cccaatagtg tactctggct gttgcgtttt ccagcagtag gagaacctaa tattcaacag
     7861 tatgcacaaa acatgggcct gccccagaac cgtatcattt tttcacctgt tgctcctaaa
     7921 gaggaacacg tcaggagagg ccagctggct gatgtctgct tggacactcc actctgtaat
     7981 gggcacacca cagggatgga tgtcctctgg gcagggaccc ccatggtgac tatgccagga
     8041 gagactcttg cttctcgagt tgcagcatcc cagctcactt gcttaggttg tcttgagctt
     8101 attgctaaaa acagacaaga atatgaagac atagctgtga agctgggaac tgatctagaa
     8161 tacctgaaga aagttcgtgg caaagtctgg aagcaaagaa tatctagccc tctgttcaac
     8221 accaaacaat acacaatgga actagagcgg ctctatctac agatgtggga gcattatgca
     8281 gctggcaaca aacctgacca catgattaag cctgttgaag tcactgagtc agcataataa
     8341 ctcgagatat ctagacccag ctttgttcag ctttcttgta caaagtggtt gatatccagc
     8401 acagtggcgg ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8461 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8521 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8581 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8641 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8701 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8761 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     8821 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8881 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8941 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9001 ctccccgcct ggaattctgc agatatccgg actagtgatc taattctacc gggtagggga
     9061 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     9121 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     9181 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     9241 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     9301 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     9361 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     9421 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     9481 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     9541 gggcctttcg acctgcagcc aatatgggat cggccattga acaagatgga ttgcacgcag
     9601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     9661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     9721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     9781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     9841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     9901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     9961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
    10021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
    10081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
    10141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
    10201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
    10261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
    10321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatccgtcga
    10381 gggcccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    10441 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
    10501 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctgcct
    10561 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10621 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10681 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
    10741 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
    10801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10861 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
    10921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    11041 ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
    11101 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    11161 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    11221 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
    11281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    11341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    11401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    11461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    11521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    11581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    11641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    11701 taacgtttac aatttcc
//
