LOCUS       pSUI-Psyn-sfGFP         4214 bp    DNA     circular     13-MAY-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13339513|
COMMENT     VNTDBDATE|-13339513|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSUI-Psyn-sfGFP|
COMMENT     VNTAUTHORNAME|tanshiyi|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(3305..3964)
                     /vntifkey="4"
                     /label=CmR
     misc_feature    2335..3008
                     /vntifkey="21"
                     /label=pUC
     CDS             942..2021
                     /vntifkey="4"
                     /label=lacI
     misc_feature    865..941
                     /vntifkey="21"
                     /label=native\promoter\and\RBS
     primer          810..839
                     /vntifkey="27"
                     /label=PstI-LacI-F
     primer          complement(2133..2162)
                     /vntifkey="27"
                     /label=BglII-LacI-R
     misc_feature    1..23
                     /vntifkey="21"
                     /label=lacO
     misc_feature    24..28
                     /vntifkey="21"
                     /label=-35
     misc_feature    31..45
                     /vntifkey="21"
                     /label=lacO
     misc_feature    48..52
                     /vntifkey="21"
                     /label=-10
     CDS             77..796
                     /vntifkey="4"
                     /label=sfGFP
     misc_feature    59..70
                     /vntifkey="21"
                     /label=B0034_RBS
BASE COUNT     1078 a      1054 c      1091 g       991 t 
ORIGIN
        1 ggaattgtga gcggataaca attttgacgg tgtgagcgga taacagtaca gtggatccaa 
       61 agaggagaaa aagcttatgc gtaaaggcga agaactgttc acgggcgtag ttccgattct 
      121 ggtcgagctg gacggcgatg tgaacggtca taagtttagc gttcgcggtg aaggtgaggg 
      181 cgacgcgacc aacggcaaac tgaccctgaa gttcatctgc accaccggta aactgccggt 
      241 gccttggccg accttggtga cgacgttgac gtatggcgtg cagtgttttg cgcgttatcc 
      301 ggaccacatg aaacaacacg atttcttcaa atctgcgatg ccggagggtt acgtccagga 
      361 gcgtaccatt tccttcaagg atgatggcta ctacaaaact cgcgcagagg ttaagtttga 
      421 aggtgacacg ctggtcaatc gtatcgaatt gaagggtatc gactttaaag aggatggtaa 
      481 cattctgggc cataaactgg agtataactt caacagccat aatgtttaca ttacggcaga 
      541 caagcaaaag aacggcatca aggccaattt caagattcgc cacaatgttg aggacggtag 
      601 cgtccaactg gccgaccatt accagcagaa caccccaatt ggtgacggtc cggttttgct 
      661 gccggataat cactatctga gcacccaaag cgtgctgagc aaagatccga acgaaaaacg 
      721 tgatcacatg gtcctgctgg aatttgtgac cgctgcgggc atcacccacg gtatggacga 
      781 gctgtataag taatgatact agtagcggcc gctgcagtgc ttcctaatgc aggagtcgca 
      841 taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg 
      901 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat 
      961 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg 
     1021 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt 
     1081 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg 
     1141 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 
     1201 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 
     1261 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 
     1321 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat 
     1381 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta 
     1441 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 
     1501 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 
     1561 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc 
     1621 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 
     1681 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 
     1741 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 
     1801 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 
     1861 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 
     1921 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     1981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     2041 gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac 
     2101 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgagatct 
     2161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     2221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     2281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     2341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     2461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     2521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     2581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     2641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     2701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     2761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     2821 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     2881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     2941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     3001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     3061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     3121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     3181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     3241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagtcga 
     3301 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 
     3361 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct 
     3421 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 
     3481 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca 
     3541 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 
     3601 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 
     3661 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 
     3721 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 
     3781 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 
     3841 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 
     3901 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 
     3961 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 
     4021 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca 
     4081 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 
     4141 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg 
     4201 cggccgcttc taga 
//
