LOCUS Exported 3899 bp ds-DNA circular SYN 06-SEP-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3899) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jun 1, 2020 from SnapGene Viewer 5.1.3 https://www.snapgene.com COMMENT Insert is blunt cloned at beginning or end of sequence FEATURES Location/Qualifiers source 1..3899 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..18 /label=M13-fwd primer_bind 17..44 /label=T7 misc_feature 122..128 /label=SapI misc_feature 130..132 /label=GGT overlap misc_feature 133..222 /label=30x linker misc_feature 223..225 /label=ACG overlap misc_feature 227..233 /label=SapI /label=SapI(2) misc_feature 362..368 /label=SapI /label=SapI(1) misc_feature 370..372 /label=GGT overlap /label=GGT overlap(1) misc_feature 463..465 /label=ACG overlap /label=ACG overlap(1) misc_feature 467..473 /label=SapI /label=SapI(3) primer_bind complement(574..599) /label=SP6 primer_bind complement(605..625) /label=M13-rev misc_binding complement(631..653) /label=LacO rep_origin complement(993..1675) /direction=LEFT /label=ColE1 origin CDS complement(2318..3109) /label=Kan/neoR CDS complement(3464..3673) /label=ccdB ORIGIN 1 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggccctcta 61 gatgcatgct cgagcggccg ccagtgtgat ggatatctgc agaattcgcc cttattctgt 121 ggctcttcgg gtggtggcgg tggatcggga ggaggaggtt cgggtggcgg aggcagtgga 181 ggtggcggaa gtggcggcgg tggttcagga ggaggcggat ccacgcgaag agcgtttgat 241 gcctggcagt ttatggcggg atcgaggatt accactacgc tacgtgacta cgctgatgta 301 cagatccata ctgcatgttc attgacgatc tacctttcct gcgttatccc ctgattctgt 361 ggctcttcgg gtggtggcgg tggatcggga ggaggaggtt cgggtggcgg aggcagtgga 421 ggtggcggaa gtggcggcgg tggttcagga ggaggcggat ccacgcgaag agcgtttgat 481 gaagggcgaa ttcaggcctg aattccagca cactggcggc cgttactagt ggatccgagc 541 tcggtaccaa gcttgatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg 601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 961 aaaggccagc aaaagcccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 1201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 1441 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttag cacgtgtcag 1741 tcctgctcct cggccacgaa gtgcacgcag ttgccggccg ggtcgcgcag ggcgaactcc 1801 cgcccccacg gctgctcgcc gatctcggtc atggccggcc cggaggcgtc ccggaagttc 1861 gtggacacga cctccgacca ctcggcgtac agctcgtcca ggccgcgcac ccacacccag 1921 gccagggtgt tgtccggcac cacctggtcc tggaccgcgc tgatgaacag ggtcacgtcg 1981 tcccggacca caccggcgaa gtcgtcctcc acgaagtccc gggagaaccc gagccggtcg 2041 gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga gcaccggaac ggcactggtc 2101 aacttggcca tggtggccct cctcacgtgc tattattgaa gcatttatca gggttattgt 2161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2221 acatttcccc gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga 2281 gaaaataccg catcaggaaa ttgtaagcgt taataattca gaagaactcg tcaagaaggc 2341 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt 2401 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat 2461 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca 2521 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca 2581 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca 2641 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt 2701 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat 2761 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg 2821 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg 2881 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat 2941 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc 3001 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc 3061 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg 3121 atcctcatcc tgtctcttga tcagagcttg atcccctgcg ccatcagatc cttggcggcg 3181 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg 3241 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc 3301 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt 3361 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgaaaagga 3421 tctaggtgaa gatccttttt gataatctca tgcctgacat ttatattccc cagaacatca 3481 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga 3541 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga 3601 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca 3661 gggggatcac catccgtcgc cccggcgtgt caataatatc actctgtaca tccacaaaca 3721 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgccgtacg tataggctgc 3781 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 3841 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgt //