LOCUS       Exported                9030 bp ds-DNA     circular SYN 20-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 1, 2020 from SnapGene Viewer 5.1.3
            https://www.snapgene.com
COMMENT     /Users/jordanqward/Dropbox/Postdoc material to sync 
            (jordan.ward@ucsf.edu)/Sequences/Vectors/B1-B2 entry 
            vectors/template.ape from 1 to 2550
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     570..576
                     /locus_tag="AttL1"
                     /label=AttL1
     misc_feature    577..583
                     /locus_tag="SapI"
                     /label=SapI
     misc_feature    585..587
                     /locus_tag="GCG overlap"
                     /label=GCG overlap
     misc_feature    588..677
                     /locus_tag="30x linker NTslot"
                     /label=30x linker NTslot
     misc_feature    678..680
                     /locus_tag="ATG Overlap"
                     /label=ATG Overlap
     CDS             678..680
                     /locus_tag="mNeonGreen"
                     /label=mNeonGreen
     misc_feature    681..1523
                     /locus_tag="mScarlet-I-GLO"
                     /label=mScarlet-I-GLO
     misc_feature    1524..1526
                     /locus_tag="AAG overlap"
                     /label=AAG overlap
     intron          1527..1537
                     /locus_tag="intron(1)"
                     /label=intron(1)
     misc_feature    1538..1571
                     /locus_tag="lox511I(1)"
                     /label=lox511I
                     /label=lox511I(1)
     intron          1572..1577
                     /locus_tag="intron(2)"
                     /label=intron(2)
     misc_feature    1578..1580
                     /locus_tag="spacer"
                     /label=spacer
     misc_feature    1581..1610
                     /locus_tag="myc"
                     /label=myc
     terminator      1614..1994
                     /locus_tag="let-858 Terminator"
                     /label=let-858 Terminator
     promoter        1995..2364
                     /locus_tag="Psqt1"
                     /label=Psqt1
     5'UTR           join(2279..2306,2363..2364)
                     /locus_tag="sqt-1 5'UTR"
                     /label=sqt-1 5'UTR
     CDS             join(2365..2505,2553..3386)
                     /locus_tag="SQT-1"
                     /label=SQT-1
     misc_difference 2616
                     /locus_tag="e1350"
                     /label=e1350
     3'UTR           3387..3423
                     /locus_tag="sqt-1 3'UTR"
                     /label=sqt-1 3'UTR
     3'UTR           complement(3424..3755)
                     /locus_tag="tbb-2 3'UTR"
                     /label=tbb-2 3'UTR
     CDS             complement(join(3759..4000,4052..4422,4474..4889))
                     /locus_tag="Cre"
                     /label=Cre
     promoter        complement(4890..5289)
                     /locus_tag="hsp16-41 promoter"
                     /label=hsp16-41 promoter
     promoter        5290..5652
                     /locus_tag="Prps-0"
                     /label=Prps-0
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink; 
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data 
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
     CDS             5695..6720
                     /locus_tag="hygR"
                     /label=hygR
                     /note="hygromycinR"
     3'UTR           6746..7097
                     /locus_tag="unc-54 3'UTR"
                     /label=unc-54 3'UTR
     misc_feature    7098..7131
                     /locus_tag="lox511I"
                     /label=lox511I
     intron          7132..7137
                     /locus_tag="intron"
                     /label=intron
     misc_feature    7138..7140
                     /locus_tag="GGT overlap"
                     /label=GGT overlap
     misc_feature    7141..7152
                     /locus_tag="spacer(1)"
                     /label=spacer
                     /label=spacer(1)
     protein_bind    7153..7182
                     /locus_tag="Myc Tag(1)"
                     /label=Myc Tag
                     /label=Myc Tag(1)
     protein_bind    7183..7212
                     /locus_tag="Myc Tag(2)"
                     /label=Myc Tag
                     /label=Myc Tag(2)
     protein_bind    7213..7242
                     /locus_tag="Myc Tag"
                     /label=Myc Tag
     misc_feature    7243..7245
                     /locus_tag="ACG overlap"
                     /label=ACG overlap
     misc_feature    7247..7253
                     /locus_tag="SapI(1)"
                     /label=SapI
                     /label=SapI(1)
     primer_bind     complement(7271..7292)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(7291..7311)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     CDS             7424..8230
                     /locus_tag="KanR"
                     /label=KanR
     rep_origin      8300..8986
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcggcgggt ggcggtggat
      601 cgggaggagg aggttcgggt ggcggaggca gtggaggtgg cggaagtggc ggcggtggtt
      661 caggaggagg cggatccatg gtctccaaag gagaagctgt gatcaaggag ttcatgcgct
      721 tcaaggttca catggaagga agcatgaatg gtcacgagtt cgaaatcgaa ggagaagggg
      781 agggccgccc gtacgaggga actcagaccg ccaagctgaa ggtcaccaag ggaggtaagt
      841 ttgtgataat ccaatttcaa ttcgcaatgg tcatcgtttt ttcaggacca cttccattct
      901 catgggatat tctctcccca caattcatgt acggctcccg tgctttcatc aaacacccag
      961 ccgacattcc agattactac aagcaatctt tcccagaagg cttcaagtgg gagcgtgtga
     1021 tgaacttcga ggatggtgga gcagttacag taactcagga cacttctctc gaggtaagtt
     1081 tttactccgc ttttaacaat ggttgtttga catcattttt tcaggatggc actttgatct
     1141 acaaggtcaa gctccgtggt accaatttcc caccagatgg accagttatg cagaagaaga
     1201 cgatgggatg ggaggcttcc accgaacgat tgtacccaga agatggagtt ctcaagggag
     1261 acatcaaaat ggcgttacgc ctcaaggacg gaggtaagtt tgatgaaacg gtttcgtctt
     1321 atatacacta atggtacttt tcaggacgtt acctggcgga tttcaagacc acatacaagg
     1381 caaagaagcc agttcaaatg ccaggagcat ataacgttga ccgcaagctt gatattactt
     1441 cccataatga agactacaca gttgtagaac aatacgaacg atccgaggga cgtcattcga
     1501 ccggaggaat ggacgagctg tacaaggtaa gtttaaaata acttcgtata atgtatacta
     1561 tacgaagtta ttttcaggga gagcaaaagc ttatctccga ggaggacctc taaggatgat
     1621 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     1681 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     1741 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     1801 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     1861 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     1921 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     1981 aggaaggaaa ctagctgccc tcgattgtct atcttccatt ctcaccaagt ttcaacaact
     2041 ttattcagtc attcttctct ggttcatttc ccaaccaatc ttcccgaata aagcccatct
     2101 gtcctttcgg ccacattcct cacctacatc cgtactcctt atctcccgtt cttatccagt
     2161 cttttatcgt ctctgcgtct cgcacactgt ctcgtcggta atgtaaaaaa aaaacaaaaa
     2221 aaacttgaaa acttgcggaa agatttccta ataaaaggag acgaatccgg taggaaatcg
     2281 acaagtctct gcttctcgaa gactaggtga gttacatttc tttggccgaa ttattttgaa
     2341 atcctaaatt tgataatttc agagatgtct gtaaaacttg cgtgttatgt gacggcttcg
     2401 gtcactgtcg ccactcttat ggtatgcttc atgaccatgt caaccatcta ctcggaggtt
     2461 gatggattca gagagaagct tgataccgag atgaatgtgt tcagagtgag ttaaattata
     2521 ttttgaattt taaataattt ttaattttct agcaatctac caatggattg tggaaggaca
     2581 tagttgtcat cggaagatct agcaagcgtg tccgttgtca atatgaagag accaacgcta
     2641 ccccaactcc acatgctgat ggatccccat ctgctccacc aggtcaacca ccagcagttc
     2701 caccagtctt caaccagcca aagactccaa atggagccaa tggaaatgga ccaacctgca
     2761 actgcaatgc tgataacaag tgcccagctg gaccatccgg accaaaggga gttccaggag
     2821 ttccaggact cgacggagtt ccaggacttg acggtgttcc aggagttgga gctgatgata
     2881 tcgctccaca acgcgagtct gtcggatgct tcacttgccc acaaggacca gttggaccac
     2941 caggagctct tggaagacca ggaccacgtg gacttccagg accaagagga caaaatggaa
     3001 acccaggaag agatggacaa ccaggacatc caggagagca aggatcatcc ggtcaaatcg
     3061 gaaagatcgg agagccagga ccaccaggag agaagggacg cgacgccgag catccaatcg
     3121 gaagaccagg accaaaggga ccaagaggag atcaaggacc aacaggacca gctggacaga
     3181 acggtcttca cggaccacca ggagagccag gaaccgttgg accagaagga ccatctggaa
     3241 agcaaggacg tcaaggacca gacggaaccc agggagagac tggaccagac ggaagaccag
     3301 gaaaggatgc cgagtactgc cagtgcccag acaagtctcc accatcagag gctgtcaacg
     3361 ccaaccgtgg atacagaaat atctaaattg ttggtgtttt ctaataaaat atttgagatt
     3421 cagtgagact tttttcttgg cggcacaata aagtttcctc tttttgttgg gtcactcaac
     3481 gaacacaata caaatatttg agcgctcgca tttttctcaa cctttcgggt ttaaagcact
     3541 tcaaaatgga attgaagaga gattgtgaaa gtacatcaga cagaatcaca ttttgatagc
     3601 attcacttca ctcagatgca aacttttcag tgactataaa aagtcataaa ataataacat
     3661 taaaaaagca aataaattag cgagagaatt ttttgacaaa aagaaagaag agtgatagag
     3721 aagaagggaa tgcttgaaag gattttgcat ttatcttaat caccgtcttc gagaagacga
     3781 accatggcgc cagtttcaga atccaagttt ctaatatagt tcatcacaat gttgacgttg
     3841 gtccaacctc cggcttgcat aatctctggg atggaaactc cagcgcgagc catgtcacgg
     3901 gcagctccga cacgggcgga atgtccagac caagccaagt aacgttgtcc ggaatcatcc
     3961 ttggctccgt agatgagacg gtgggtagct tcgaagattc ctgaaaattt attttcagct
     4021 tattcttagc taatatgttg tttaaactta ccttcaagag ctcgagtcga gagttgtgat
     4081 gttgcactcg gggcagcaac tccgttcttg cgaacacgac agaacaggta gttgtttgga
     4141 tcatcggcga ctccggaaac cgaaatccag cgctcaacga gcttggtaac tccaagggag
     4201 agcgcttttt cgactccggc agtcgagacc agcgtttttg tacgtccgat gtggatgagc
     4261 attcttcctc cgtctgtgcg actaatatcc ttgacgcgaa tgcgggcaat ttcggcgata
     4321 cggagaagtg tgttgtaagc aattccgagg aaggcgaggt ttcggatgtc ctggcaacga
     4381 tcggaatttt ccataagaga acggacctga tcgaagtcgg ttctgaaaat ttcgtcaagt
     4441 cttaggattg aatacattaa tatttaaact tacctttcga aggcgagggc ttgcttggct
     4501 ctctctccag catcaacatt ttccttgcgg atacgtctca ttacgagaga tacggcatta
     4561 gaatctgatg ggcgtggaag tccagatcgg cgatggagca tattgagctg tccgagatgt
     4621 tgttggatcg tcttcaccgc caatccacga gcttggaggt agagcaagta gtcacgaacg
     4681 tcttctggct cggctgggaa ccacttgcgg ttgttaagct tgcaccaggc agcccaagaa
     4741 cggcacactg agagaagcat cttccaggtg tgctcggaga aggcttgtct gtcacggaac
     4801 atatccatga ggttcttgcg aacttcatca cttgtcgcgt caactggaag tgctggaaga
     4861 ttttgatgaa cagtgagaag atttgacata ttttcgaagt tttttagatg cactagaaca
     4921 aagcgtgttg gcttcctctg agcccgcttt ccttatatac ccgcattctg cagccttaca
     4981 gaatgttcta gaaggtccta gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag
     5041 acgcagacgg aaaatgtatc tgggtctctt tattgtgtac actacttttc catgtaccga
     5101 atgtgagtcg ccctcctttt gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat
     5161 agtataaata ccgttgaaaa taaataccga acaacatttg ctctaattgt gaaattagaa
     5221 atcttcaaac tataatcatg tcactttacc actatttccg tccagctcaa cgttccgttt
     5281 ttggtgatca tttttgcttt cgtcgtaaat ctacacacgc gtctcttccg tgcgagagtc
     5341 caagccagca gccaaattcg ttgactgagt attcaacgtt tatacgttgt cggcaacgag
     5401 aaataggaaa atgcatcggg aaatgttctt ttttcgattt tttccaaggt tttgacaaat
     5461 tttaccacga attttgctat gttttcaatt aaaaaatatg ttattcaact gtttctatga
     5521 ggaaaataag gctttgcatg taattttctt attcagcata atttttaatt aatttgaatt
     5581 ttctgtccta acgtttattt tgttttcttg gttatgactg atctgaaatt aatttttgaa
     5641 ttttaaggta atatgtcagg cggtgccgca agtttgtaca aaaaagcagg ctccatgaaa
     5701 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     5761 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     5821 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     5881 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     5941 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     6001 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     6061 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     6121 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     6181 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     6241 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     6301 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     6361 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     6421 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     6481 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     6541 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     6601 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     6661 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     6721 acccagcttt cttgtacaaa gtgggtccaa ttactcttca acatccctac atgctctttc
     6781 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     6841 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     6901 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     6961 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     7021 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     7081 tatgaccgca atttttaata acttcgtata atgtatacta tacgaagtta ttttcagggt
     7141 ggagccggat ctgaacaaaa acttatttct gaagaggatc ttgagcaaaa gctcatctcc
     7201 gaggaggacc tcgagcagaa gttgatcagc gaggaagact tgacgcgaag agcgccatcc
     7261 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     7321 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     7381 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     7441 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     7501 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     7561 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     7621 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     7681 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     7741 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     7801 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     7861 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     7921 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     7981 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     8041 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     8101 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     8161 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     8221 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     8281 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     8341 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8401 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8461 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8521 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8581 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8641 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8701 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8761 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8821 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8881 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8941 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9001 ggccttttgc tggccttttg ctcacatgtt
//
