LOCUS       Exported                3311 bp ds-DNA     circular SYN 20-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 1, 2020 from SnapGene Viewer 5.1.3
            https://www.snapgene.com
COMMENT     /Users/jordanqward/Dropbox/Postdoc material to sync 
            (jordan.ward@ucsf.edu)/Sequences/Vectors/B1-B2 entry 
            vectors/template.ape from 1 to 2550
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     570..576
                     /locus_tag="AttL1"
                     /label=AttL1
     misc_feature    577..583
                     /locus_tag="SapI"
                     /label=SapI
     misc_feature    585..587
                     /locus_tag="GCG overlap"
                     /label=GCG overlap
     misc_feature    588..677
                     /locus_tag="30x linker NTslot"
                     /label=30x linker NTslot
     CDS             678..680
                     /locus_tag="mNeonGreen"
                     /label=mNeonGreen
     misc_feature    678..680
                     /locus_tag="ATG Overlap"
                     /label=ATG Overlap
     misc_feature    681..1523
                     /locus_tag="mScarlet-I-GLO"
                     /label=mScarlet-I-GLO
     misc_feature    1524..1526
                     /locus_tag="AAG overlap"
                     /label=AAG overlap
     misc_feature    1528..1534
                     /locus_tag="SapI(1)"
                     /label=SapI
                     /label=SapI(1)
     primer_bind     complement(1552..1573)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(1572..1592)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     CDS             1705..2511
                     /locus_tag="KanR"
                     /label=KanR
     rep_origin      2581..3267
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgctc ttcggcgggt ggcggtggat
      601 cgggaggagg aggttcgggt ggcggaggca gtggaggtgg cggaagtggc ggcggtggtt
      661 caggaggagg cggatccatg gtctccaaag gagaagctgt gatcaaggag ttcatgcgct
      721 tcaaggttca catggaagga agcatgaatg gtcacgagtt cgaaatcgaa ggagaagggg
      781 agggccgccc gtacgaggga actcagaccg ccaagctgaa ggtcaccaag ggaggtaagt
      841 ttgtgataat ccaatttcaa ttcgcaatgg tcatcgtttt ttcaggacca cttccattct
      901 catgggatat tctctcccca caattcatgt acggctcccg tgctttcatc aaacacccag
      961 ccgacattcc agattactac aagcaatctt tcccagaagg cttcaagtgg gagcgtgtga
     1021 tgaacttcga ggatggtgga gcagttacag taactcagga cacttctctc gaggtaagtt
     1081 tttactccgc ttttaacaat ggttgtttga catcattttt tcaggatggc actttgatct
     1141 acaaggtcaa gctccgtggt accaatttcc caccagatgg accagttatg cagaagaaga
     1201 cgatgggatg ggaggcttcc accgaacgat tgtacccaga agatggagtt ctcaagggag
     1261 acatcaaaat ggcgttacgc ctcaaggacg gaggtaagtt tgatgaaacg gtttcgtctt
     1321 atatacacta atggtacttt tcaggacgtt acctggcgga tttcaagacc acatacaagg
     1381 caaagaagcc agttcaaatg ccaggagcat ataacgttga ccgcaagctt gatattactt
     1441 cccataatga agactacaca gttgtagaac aatacgaacg atccgaggga cgtcattcga
     1501 ccggaggaat ggacgagctg tacaagcgaa gagcgccatc cagctgatat cccctatagt
     1561 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1621 tgatgttaca ttgcacaaga taaaataata tcatcatgaa caataaaact gtctgcttac
     1681 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1741 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1801 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1861 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1921 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1981 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2041 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2101 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2161 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2221 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2281 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2341 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2401 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2461 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2521 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2581 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3301 gctcacatgt t
//
