LOCUS       Exported                4025 bp ds-DNA     circular SYN 29-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 1, 2020 from SnapGene Viewer 5.1.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     535..553
                     /label=9_M13 forward
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_recomb     570..576
                     /locus_tag="AttL1"
                     /label=AttL1
     misc_recomb     570..576
                     /locus_tag="AttP1"
                     /label=AttP1
     misc_feature    577..601
                     /locus_tag="pes10.delta-181"
                     /label=pes10.delta-181
     misc_feature    582..762
                     /locus_tag="pes-10 minimal promoter"
                     /label=pes-10 minimal promoter
     misc_feature    738..760
                     /locus_tag="pes10.Tsa"
                     /label=pes10.Tsa
     misc_feature    879..881
                     /locus_tag="start codon"
                     /label=start codon
     misc_feature    882..902
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
     misc_feature    909..1065
                     /locus_tag="mScarlet(3)"
                     /label=mScarlet
                     /label=mScarlet(3)
     misc_feature    1066..1116
                     /locus_tag="mScarlet-I-GLO(2)"
                     /label=mScarlet-I-GLO
                     /label=mScarlet-I-GLO(2)
     misc_feature    1117..1262
                     /locus_tag="mScarlet(2)"
                     /label=mScarlet
                     /label=mScarlet(2)
     misc_feature    1263..1313
                     /locus_tag="mScarlet-I-GLO"
                     /label=mScarlet-I-GLO
     misc_feature    1314..1488
                     /locus_tag="mScarlet(1)"
                     /label=mScarlet
                     /label=mScarlet(1)
     misc_feature    1489..1539
                     /locus_tag="mScarlet-I-GLO(1)"
                     /label=mScarlet-I-GLO
                     /label=mScarlet-I-GLO(1)
     misc_feature    1540..1757
                     /locus_tag="mScarlet"
                     /label=mScarlet
     misc_feature    1758..1790
                     /locus_tag="flexible linker"
                     /label=flexible linker
     misc_feature    1791..1913
                     /locus_tag="PEST"
                     /label=PEST
     terminator      1917..2248
                     /locus_tag="tbb-2\3'UTR"
                     /label=tbb-2\3'UTR
     primer_bind     complement(2266..2287)
                     /locus_tag="T7"
                     /label=T7
     primer_bind     complement(2286..2306)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(2290..2306)
                     /label=38_M13 reverse
     CDS             2419..3225
                     /locus_tag="KanR"
                     /label=KanR
     rep_origin      3295..3981
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatc gattttttgc aaattacgag
      601 cgttgtaggg ggcggagcga taggtcctat aggttttggt atatcatcat tcattcattc
      661 attggtacat tcatttaccc accttcctct ttctgagctt ctctggagtt ctgtgcttcc
      721 tttttccctt atctttatac tgtaattttt aactttcagg cattgattgg atccccggga
      781 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
      841 ggaggaccct tgcttggagg gtaccgagct cagaaaaaat gccaaagaag aagcgtaagg
      901 tcctgcagat ggtctccaag ggagaggctg tcatcaagga gttcatgcgt ttcaaggtcc
      961 acatggaggg atccatgaac ggacacgagt tcgagatcga gggagaggga gagggacgtc
     1021 catacgaggg aacccaaacc gccaagctca aggtcaccaa gggaggtaag tttgtgataa
     1081 tccaatttca attcgcaatg gtcatcgttt tttcaggacc actcccattc tcctgggaca
     1141 tcctctcccc acaattcatg tacggatccc gtgccttcat caagcaccca gccgacatcc
     1201 cagactacta caagcaatcc ttcccagagg gattcaagtg ggagcgtgtg atgaacttcg
     1261 aggtaagttt ttactccgct tttaacaatg gttgtttgac atcatttttt caggacggag
     1321 gagccgtcac cgtcacccaa gacacctccc tcgaggacgg aaccctcatc tacaaggtca
     1381 agctccgtgg aaccaacttc ccaccagacg gaccagtcat gcaaaagaag accatgggat
     1441 gggaggcctc caccgagcgt ctctacccag aggacggagt cctcaaaggt aagtttgatg
     1501 aaacggtttc gtcttatata cactaatggt acttttcagg agacatcaag atggccctcc
     1561 gtctcaagga cggaggacgt tacctcgccg acttcaagac cacctacaag gccaagaagc
     1621 cagtccaaat gccaggagcc tacaacgtcg accgtaagct cgacatcacc tcccacaacg
     1681 aggactacac cgtcgtcgag caatacgagc gttccgaggg acgtcactcc accggaggaa
     1741 tggacgagct ctacaagtcc ggaggatccg gaggaaccgg aggatcctct ctttctcacg
     1801 gattcccacc agctgttgct gctcaagatg atggaaccct tccaatgtct tgcgctcaag
     1861 agtctggaat ggatcgtcac ccagctgctt gcgcttccgc tcgtatcaac gtctaagata
     1921 aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     1981 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     2041 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     2101 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     2161 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     2221 ttattgtgcc gccaagaaaa aagtctcagc catccagctg atatccccta tagtgagtcg
     2281 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2341 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac
     2401 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2461 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2521 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2581 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2641 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2701 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2761 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2821 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2881 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2941 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3001 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3061 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3121 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3181 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3241 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3301 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4021 atgtt
//
