LOCUS       pSUI-SynP-CAS1sg        3578 bp    DNA     circular      2-JUN-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|-13367622|
COMMENT     VNTDBDATE|-13367626|
COMMENT     LSOWNER|
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FEATURES             Location/Qualifiers
     CDS             complement(2669..3328)
                     /vntifkey="4"
                     /label=CmR
     misc_feature    1699..2372
                     /vntifkey="21"
                     /label=pUC
     CDS             306..1385
                     /vntifkey="4"
                     /label=lacI
     misc_feature    229..305
                     /vntifkey="21"
                     /label=native\promoter\and\RBS
     primer          174..203
                     /vntifkey="27"
                     /label=PstI-LacI-F
     primer          complement(1497..1526)
                     /vntifkey="27"
                     /label=BglII-LacI-R
     CDS             79..120
                     /gene="cas9BIRE"
                     /vntifkey="4"
                     /label=cas9BIRE
     CDS             121..160
                     /gene="spy-tem"
                     /vntifkey="4"
                     /label=spy-tem
     primer          complement(137..183)
                     /vntifkey="27"
                     /label=scaffold-R
     misc_feature    1..23
                     /vntifkey="21"
                     /label=lacO
     misc_feature    24..28
                     /vntifkey="21"
                     /label=-35
     misc_feature    31..45
                     /vntifkey="21"
                     /label=lacO
     misc_feature    48..52
                     /vntifkey="21"
                     /label=-10
     misc_feature    59..78
                     /vntifkey="21"
                     /label=CAS1::N20
BASE COUNT      906 a       900 c       914 g       858 t 
ORIGIN
        1 ggaattgtga gcggataaca attttgacgg tgtgagcgga taacagtaca gtggatcctg 
       61 aactgaacgt catggaacgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg 
      121 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tactagtagc ggccgctgca 
      181 gtgcttccta atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat 
      241 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg 
      301 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 
      361 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 
      421 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 
      481 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 
      541 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 
      601 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 
      661 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 
      721 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 
      781 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 
      841 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 
      901 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 
      961 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 
     1021 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 
     1081 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 
     1141 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 
     1201 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 
     1261 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 
     1321 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 
     1381 ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga 
     1441 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 
     1501 atcgtcgccg cacttatgag atctattaat gaatcggcca acgcgcgggg agaggcggtt 
     1561 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     1621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     1681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     1741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     1801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     1861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     1921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     1981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     2041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     2101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     2161 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     2221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     2281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     2341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     2401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     2461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     2521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     2581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     2641 ctgcaatgat accgcgagag tcgacaaatt acgccccgcc ctgccactca tcgcagtact 
     2701 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 
     2761 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 
     2821 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 
     2881 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 
     2941 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 
     3001 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 
     3061 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat 
     3121 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 
     3181 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 
     3241 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 
     3301 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 
     3361 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 
     3421 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta 
     3481 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc 
     3541 gctaaggatg atttctggaa ttcgcggccg cttctaga 
//
