LOCUS       hu6_HGPS_sgRNA_e        7380 bp    DNA     circular UNA 02-JUN-2020
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     repeat_region   complement(<1..106)
                     /standard_name="3'-ITR pSub201(+)"
     misc_feature    3..2955
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCC"
     misc_feature    122..282
                     /note="Geneious type: reporter"
                     /standard_name="lacZ_a reporter"
     primer_bind     complement(125..142)
                     /standard_name="Eton M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="Genewiz M13F(-21)"
     primer_bind     complement(125..142)
                     /standard_name="QB0046 M13 (-21) Forward"
     primer_bind     complement(125..141)
                     /standard_name="M13-F (-20)"
     primer_bind     complement(134..156)
                     /standard_name="QB0049 M13/pUC Forward"
     primer_bind     complement(145..168)
                     /standard_name="Genewiz M13F(-47)"
     primer_bind     complement(145..162)
                     /standard_name="Genewiz M13F(-41)"
     primer_bind     complement(145..161)
                     /standard_name="Eton M13F(-40)"
     primer_bind     complement(145..161)
                     /standard_name="M13-F (-40)"
     primer_bind     complement(145..161)
                     /standard_name="QB0047 M13 (-40)"
     primer_bind     complement(146..167)
                     /standard_name="M13-F (-46)"
     primer_bind     complement(209..228)
                     /standard_name="LacZ-R (EtonSeq)"
     primer_bind     209..228
                     /standard_name="834_LacZ-fwd1"
     misc_feature    300..606
                     /note="Geneious type: origin of replication"
                     /standard_name="f1 origin"
     primer_bind     complement(420..439)
                     /standard_name="835_LacZ-rev1"
     promoter        800..828
                     /standard_name="amp prom"
     misc_feature    870..1730
                     /note="Geneious type: marker"
                     /standard_name="amp marker"
     CDS             870..1730
                     /standard_name="Amp-R"
     primer_bind     complement(995..1022)
                     /standard_name="563_Amp_Rev"
     primer_bind     995..1022
                     /standard_name="562_Amp_Fwd"
     primer_bind     complement(1088..1107)
                     /standard_name="QB0005 Amp-R"
     primer_bind     complement(1305..1324)
                     /standard_name="ACK563"
     misc_feature    1885..2504
                     /note="Geneious type: origin of replication"
                     /standard_name="pBR322 origin"
     primer_bind     1969..1988
                     /standard_name="832_BR322-fwd1"
     primer_bind     2122..2141
                     /standard_name="QB0767 RepO-R"
     primer_bind     complement(2150..2169)
                     /standard_name="833_BR322-rev1"
     primer_bind     complement(2248..2266)
                     /standard_name="QB0766 RepO-F"
     primer_bind     2409..2428
                     /standard_name="BR322-F"
     primer_bind     2498..2519
                     /standard_name="11_pFYF_Seq_Rev"
     primer_bind     2589..2608
                     /standard_name="VR"
     primer_bind     2643..2660
                     /standard_name="QB0039 L4440"
     primer_bind     2787..2806
                     /standard_name="Lac-F (EtonSeq)"
     primer_bind     2787..2806
                     /standard_name="836_Lac-F"
     promoter        2813..2842
                     /standard_name="lac prom"
     primer_bind     2855..2878
                     /standard_name="M13-R (-46)"
     primer_bind     2855..2878
                     /standard_name="QB0108 Tac promoter"
     primer_bind     2856..2878
                     /standard_name="QB0050 M13/pUC Reverse"
     primer_bind     2875..2891
                     /standard_name="M13-R (-26)"
     primer_bind     2875..2891
                     /standard_name="Genewiz M13R"
     primer_bind     2875..2891
                     /standard_name="Eton M13R Reverse"
     primer_bind     2875..2891
                     /standard_name="QB0048 M13 Reverse"
     primer_bind     complement(2877..2903)
                     /standard_name="654_CAG_fwd"
     repeat_region   2918..3047
                     /standard_name="5'-ITR pSub201(+)"
     primer_bind     3035..3088
                     /standard_name="911_px601_Xba/Kpn-U6-removal"
     misc_feature    3060..7346
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CTCGAGGCGTTGACATTGATTATTGACTAGTTATTAATAGTA
                     ATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT
                     ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAA
                     TGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGA
                     GTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACG
                     CCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGA
                     CCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCAT
                     GGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGA
                     TTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAAC
                     GGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCG
                     TGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTACCGGTGCCACCATG
                     GCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCAAGCGGAACT
                     ACATCCTGGGCCTGGACATCGGCATCACCAGCGTGGGCTACGGCATCATCGACTACGA
                     GACACGGGACGTGATCGATGCCGGCGTGCGGCTGTTCAAAGAGGCCAACGTGGAAAAC
                     AACGAGGGCAGGCGGAGCAAGAGAGGCGCCAGAAGGCTGAAGCGGCGGAGGCGGCATA
                     GAATCCAGAGAGTGAAGAAGCTGCTGTTCGACTACAACCTGCTGACCGACCACAGCGA
                     GCTGAGCGGCATCAACCCCTACGAGGCCAGAGTGAAGGGCCTGAGCCAGAAGCTGAGC
                     GAGGAAGAGTTCTCTGCCGCCCTGCTGCACCTGGCCAAGAGAAGAGGCGTGCACAACG
                     TGAACGAGGTGGAAGAGGACACCGGCAACGAGCTGTCCACCAAAGAGCAGATCAGCCG
                     GAACAGCAAGGCCCTGGAAGAGAAATACGTGGCCGAACTGCAGCTGGAACGGCTGAAG
                     AAAGACGGCGAAGTGCGGGGCAGCATCAACAGATTCAAGACCAGCGACTACGTGAAAG
                     AAGCCAAACAGCTGCTGAAGGTGCAGAAGGCCTACCACCAGCTGGACCAGAGCTTCAT
                     CGACACCTACATCGACCTGCTGGAAACCCGGCGGACCTACTATGAGGGACCTGGCGAG
                     GGCAGCCCCTTCGGCTGGAAGGACATCAAAGAATGGTACGAGATGCTGATGGGCCACT
                     GCACCTACTTCCCCGAGGAACTGCGGAGCGTGAAGTACGCCTACAACGCCGACCTGTA
                     CAACGCCCTGAACGACCTGAACAATCTCGTGATCACCAGGGACGAGAACGAGAAGCTG
                     GAATATTACGAGAAGTTCCAGATCATCGAGAACGTGTTCAAGCAGAAGAAGAAGCCCA
                     CCCTGAAGCAGATCGCCAAAGAAATCCTCGTGAACGAAGAGGATATTAAGGGCTACAG
                     AGTGACCAGCACCGGCAAGCCCGAGTTCACCAACCTGAAGGTGTACCACGACATCAAG
                     GACATTACCGCCCGGAAAGAGATTATTGAGAACGCCGAGCTGCTGGATCAGATTGCCA
                     AGATCCTGACCATCTACCAGAGCAGCGAGGACATCCAGGAAGAACTGACCAATCTGAA
                     CTCCGAGCTGACCCAGGAAGAGATCGAGCAGATCTCTAATCTGAAGGGCTATACCGGC
                     ACCCACAACCTGAGCCTGAAGGCCATCAACCTGATCCTGGACGAGCTGTGGCACACCA
                     ACGACAACCAGATCGCTATCTTCAACCGGCTGAAGCTGGTGCCCAAGAAGGTGGACCT
                     GTCCCAGCAGAAAGAGATCCCCACCACCCTGGTGGACGACTTCATCCTGAGCCCCGTC
                     GTGAAGAGAAGCTTCATCCAGAGCATCAAAGTGATCAACGCCATCATCAAGAAGTACG
                     GCCTGCCCAACGACATCATTATCGAGCTGGCCCGCGAGAAGAACTCCAAGGACGCCCA
                     GAAAATGATCAACGAGATGCAGAAGCGGAACCGGCAGACCAACGAGCGGATCGAGGAA
                     ATCATCCGGACCACCGGCAAAGAGAACGCCAAGTACCTGATCGAGAAGATCAAGCTGC
                     ACGACATGCAGGAAGGCAAGTGCCTGTACAGCCTGGAAGCCATCCCTCTGGAAGATCT
                     GCTGAACAACCCCTTCAACTATGAGGTGGACCACATCATCCCCAGAAGCGTGTCCTTC
                     GACAACAGCTTCAACAACAAGGTGCTCGTGAAGCAGGAAGAAAACAGCAAGAAGGGCA
                     ACCGGACCCCATTCCAGTACCTGAGCAGCAGCGACAGCAAGATCAGCTACGAAACCTT
                     CAAGAAGCACATCCTGAATCTGGCCAAGGGCAAGGGCAGAATCAGCAAGACCAAGAAA
                     GAGTATCTGCTGGAAGAACGGGACATCAACAGGTTCTCCGTGCAGAAAGACTTCATCA
                     ACCGGAACCTGGTGGATACCAGATACGCCACCAGAGGCCTGATGAACCTGCTGCGGAG
                     CTACTTCAGAGTGAACAACCTGGACGTGAAAGTGAAGTCCATCAATGGCGGCTTCACC
                     AGCTTTCTGCGGCGGAAGTGGAAGTTTAAGAAAGAGCGGAACAAGGGGTACAAGCACC
                     ACGCCGAGGACGCCCTGATCATTGCCAACGCCGATTTCATCTTCAAAGAGTGGAAGAA
                     ACTGGACAAGGCCAAAAAAGTGATGGAAAACCAGATGTTCGAGGAAAAGCAGGCCGAG
                     AGCATGCCCGAGATCGAAACCGAGCAGGAGTACAAAGAGATCTTCATCACCCCCCACC
                     AGATCAAGCACATTAAGGACTTCAAGGACTACAAGTACAGCCACCGGGTGGACAAGAA
                     GCCTAATAGAGAGCTGATTAACGACACCCTGTACTCCACCCGGAAGGACGACAAGGGC
                     AACACCCTGATCGTGAACAATCTGAACGGCCTGTACGACAAGGACAATGACAAGCTGA
                     AAAAGCTGATCAACAAGAGCCCCGAAAAGCTGCTGATGTACCACCACGACCCCCAGAC
                     CTACCAGAAACTGAAGCTGATTATGGAACAGTACGGCGACGAGAAGAATCCCCTGTAC
                     AAGTACTACGAGGAAACCGGGAACTACCTGACCAAGTACTCCAAAAAGGACAACGGCC
                     CCGTGATCAAGAAGATTAAGTATTACGGCAACAAACTGAACGCCCATCTGGACATCAC
                     CGACGACTACCCCAACAGCAGAAACAAGGTCGTGAAGCTGTCCCTGAAGCCCTACAGA
                     TTCGACGTGTACCTGGACAATGGCGTGTACAAGTTCGTGACCGTGAAGAATCTGGATG
                     TGATCAAAAAAGAAAACTACTACGAAGTGAATAGCAAGTGCTATGAGGAAGCTAAGAA
                     GCTGAAGAAGATCAGCAACCAGGCCGAGTTTATCGCCTCCTTCTACAACAACGATCTG
                     ATCAAGATCAACGGCGAGCTGTATAGAGTGATCGGCGTGAACAACGACCTGCTGAACC
                     GGATCGAAGTGAACATGATCGACATCACCTACCGCGAGTACCTGGAAAACATGAACGA
                     CAAGAGGCCCCCCAGGATCATTAAGACAATCGCCTCCAAGACCCAGAGCATTAAGAAG
                     TACAGCACAGACATTCTGGGCAACCTGTATGAAGTGAAATCTAAGAAGCACCCTCAGA
                     TCATCAAAAAGGGCAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAA
                     AAAGGAGGGCAGAGGATCCCTGCTAACATGTGGTGACGTCGAGGAGAATCCTGGCCCA
                     ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGG
                     ACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCAC
                     CTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGG
                     CCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACC
                     ACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCG
                     CACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAG
                     GGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCA
                     ACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGC
                     CGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGAC
                     GGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCG
                     TGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
                     CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTC
                     GGCATGGACGAGCTGTACAAGTAAAGCGGCCGAATTCCTAGAGCTCGCTGATCAGCCT
                     CGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT
                     GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCG
                     CATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGG
                     GGGAGGATTGGGAAGAGAATAGCAGGCATGCTGGGGAGGTACCTGAGGGCCTATTTCC
                     CATGATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTGGAATT
                     AATTTGACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAAT
                     TTCTTGGGTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTAC
                     CGTAACTTGAAAGTATTTCGATTTCTTGGCTTTATATATCTTGTGGAAAGGACGAAAC
                     ACCGGAGACCACGGCAGGTCTCAGTTTTAGTACTCTGGAAACAGAATCTACTAAAACA
                     AGGCAAAATGCCGTGTTTATCTCGTCAACTTGTTGGCGAGATTT"
     promoter        3064..3868
                     /standard_name="CBh promoter"
     promoter        3072..3357
                     /standard_name="CMV Enhancer"
     promoter        3370..3631
                     /standard_name="Chicken B-actin promoter"
     primer_bind     3406..3425
                     /standard_name="pCAGGS-F3-Seq"
     primer_bind     complement(3558..3577)
                     /standard_name="pCAGGS-R1-Seq"
     promoter        3608..3613
                     /standard_name="CBA TATA Box"
     promoter        3632..3771
                     /standard_name="Chicken B-actin intron"
     promoter        3638
                     /standard_name="putative cba +1"
     promoter        3726..3734
                     /standard_name="b-actin SD (MaxEnt)"
     promoter        3777..3833
                     /standard_name="MVM Intron"
     promoter        3779..3787
                     /standard_name="MVM SD"
     primer_bind     3792..3851
                     /standard_name="830_MVM-fwd-bridge2"
     primer_bind     3802..3821
                     /standard_name="743_Cbh_f_seq"
     promoter        3834..3868
                     /standard_name="MVM Intron 2"
     primer_bind     3850..3880
                     /standard_name="798_Cas9-FLAG-NLS-fwd"
     promoter        complement(3861..3865)
                     /standard_name="MVM SA (MaxEnt)"
     CDS             3938..4042
                     /standard_name="NpuC"
     primer_bind     complement(4018..4062)
                     /standard_name="902_fzCas9_NpuN-rev"
     source          4043..6427
     misc_feature    <4043..6427
                     /note="hSpCas9"
                     /note="Geneious type: CDS"
                     /standard_name="fz hSpCas9"
     primer_bind     4043..4065
                     /standard_name="841_FZ_Cas9-C11-fwd"
     primer_bind     4445..4464
                     /standard_name="LCv2_seq14"
     primer_bind     4938..4957
                     /standard_name="LCv2_seq15"
     primer_bind     5403..5421
                     /standard_name="LCv2_seq16"
     misc_feature    6440..>6469
                     /standard_name="SV40 NLS"
     primer_bind     6441..6460
                     /standard_name="919_EcoRI-BPNLS-fwd"
     misc_feature    6470..6490
                     /standard_name="SV40 NLS"
     source          <6499..>6747
                     /organism="Cloning vector pAAV-CW3SL-EGFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1476495"
     terminator      6499..6659
                     /standard_name="WPRE_Gamma"
     primer_bind     complement(6555..6575)
                     /standard_name="QB0112 WPRE-R"
     primer_bind     complement(6577..6594)
                     /standard_name="pWPTRev"
     primer_bind     complement(6727..6757)
                     /standard_name="412_CW3/bGH_rev"
     primer_bind     6738..6770
                     /standard_name="411_CW3/bGH_fwd"
     polyA_signal    <6748..6958
     primer_bind     6748..6768
                     /standard_name="435_bGH_QPCRF1"
     primer_bind     6774..6792
                     /standard_name="436_bGH_QPCRF2"
     primer_bind     complement(6897..6921)
                     /standard_name="ACK64 (end)"
     primer_bind     complement(6911..6930)
                     /standard_name="438_bGH_QPCRR2"
     primer_bind     complement(6916..6934)
                     /standard_name="439_bGH_QPCRR3"
     primer_bind     complement(6936..6955)
                     /standard_name="437_bGH_QPCRR1"
     primer_bind     6956..7004
                     /standard_name="924_3'U6-XhoI-fwd"
     primer_bind     complement(6956..6975)
                     /standard_name="920_bGH-BamHI-rev"
     misc_feature    6982..7002
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="ACK68"
     terminator      complement(6982..6988)
                     /standard_name="U6 terminator"
     primer_bind     6982..7002
                     /standard_name="223_rev_sgRNA_T7"
     ncRNA           complement(6989..7064)
                     /standard_name="RNA scaffold"
     primer_bind     complement(7030..7064)
                     /standard_name="ACK337-343 anneal"
     primer_bind     complement(7044..7064)
                     /standard_name="ACK74-78,144,148 anneal"
     primer_bind     7065..7084
                     /standard_name="Binding Region"
     primer_bind     7085..7106
                     /standard_name="Rev_sgRNA_plasmid"
     promoter        complement(7092..7348)
                     /standard_name="hU6"
     primer_bind     complement(7143..7162)
                     /standard_name="Eton LKO.1 5' (hU6 promoter)"
     primer_bind     complement(7143..7162)
                     /standard_name="QB0042 LKO.1 5'"
     primer_bind     complement(7182..7204)
                     /standard_name="LCv2_seq5"
     primer_bind     complement(7207..7226)
                     /standard_name="ACK365"
     primer_bind     complement(7207..7226)
                     /standard_name="9_ACK365"
     primer_bind     complement(7265..7289)
                     /standard_name="QB0109 TRC-F"
     primer_bind     complement(7329..7374)
                     /standard_name="913_3'U6-NotI-rev"
     misc_feature    7380^7381
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGC
                     GACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGC
                     GCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGT
                     ATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAG
                     CGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCG
                     CCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTC
                     AAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA
                     CCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACG
                     GTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAA
                     CTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCC
                     GATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTT
                     AACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATG
                     CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGC
                     TTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATG
                     TGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATAC
                     GCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCAC
                     TTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAAT
                     ATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGA
                     AGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG
                     CCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
                     TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGA
                     GTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGG
                     CGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
                     TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCA
                     TGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAA
                     CTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
                     GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAA
                     ACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATT
                     AACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
                     GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG
                     ATAAATCTGGAGCCGGTGAGCGTGGAAGCCGCGGTATCATTGCAGCACTGGGGCCAGA
                     TGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
                     GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGT
                     CAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAA
                     AAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAG
                     TTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATC
                     CTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGT
                     GGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC
                     AGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCA
                     AGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGC
                     TGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGAT
                     AAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAA
                     CGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC
                     CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGC
                     ACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCC
                     ACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAA
                     AAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAC
                     ATGT"
ORIGIN      
        1 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
       61 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta
      121 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      181 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      241 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      721 tattaacgtt tataatttca ggtggcatct ttcggggaaa tgtgcgcgga acccctattt
      781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      961 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1021 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1081 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1201 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1261 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1321 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1381 taccaaacga cgagcgtgac accacgatgc ctgtagtaat ggtaacaacg ttgcgcaaac
     1441 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1681 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1741 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2041 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctgcggtttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2701 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2761 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2821 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2881 acagctatga ccatgattac gccagattta attaaggctg cgcgctcgct cgctcactga
     2941 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
     3001 gcgagcgcgc agagagggag tggccaactc catcactagg ggttcctgcg gcctctagat
     3061 cagggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3121 ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga
     3181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     3241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt
     3301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
     3361 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     3421 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
     3481 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
     3541 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     3601 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc tgccttcgcc
     3661 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     3721 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga
     3781 ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc
     3841 tgcctgaaat cacttttttt caggttggac cggtgccacc atgaaacgga cagccgacgg
     3901 aagcgagttc gagtcaccaa agaagaagcg gaaagtcatc aagattgcta cacggaaata
     3961 cctgggaaag cagaacgtgt acgacatcgg cgtggagcgg gatcacaact tcgccctgaa
     4021 gaatggcttt atcgccagca attgcttcga ctccgtggaa atctccggcg tggaagatcg
     4081 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     4141 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     4201 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     4261 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     4321 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     4381 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     4441 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     4501 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     4561 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     4621 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     4681 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     4741 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     4801 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     4861 tctgaaggac gactccatcg ataacaaggt gctgaccaga agcgacaaga accggggcaa
     4921 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     4981 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     5041 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     5101 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     5161 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     5221 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     5281 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     5341 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     5401 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     5461 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     5521 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     5581 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     5641 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     5701 ggaccctaag aagtacggcg gcttcgtgag ccccaccgtg gcctattctg tgctggtggt
     5761 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     5821 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     5881 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     5941 ggaaaacggc cggaagagaa tgctggcctc agccagagaa ctgcagaagg gaaacgaact
     6001 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     6061 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     6121 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     6181 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     6241 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     6301 ctttgacacc accatcgacc ggaagcagta cagaagcacc aaagaggtgc tggacgccac
     6361 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     6421 aggtgactct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa
     6481 gaggaaagtc taagatctga taatcaacct ctggattaca aaatttgtga aagattgact
     6541 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6601 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
     6661 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6721 ctgttgggca ctgacaattc cgtggtgcga ctgtgccttc tagttgccag ccatctgttg
     6781 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6841 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6901 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6961 cggtgggctc tatggctcga gaaaaaaagc accgactcgg tgccactttt tcaagttgat
     7021 aacggactag ccttatttta acttgctatt tctagctcta aaacggagcc caggtgggtg
     7081 gaccggtgtt tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
     7141 ttacggtaag catatgatag tccattttaa aacataattt taaaactgca aactacccaa
     7201 gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat
     7261 taattctaat tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg
     7321 gaaataggcc ctcttcctgc ccgaccttgc ggccgcagga acccctagtg atggagttgg
//
