LOCUS       Exported               12279 bp ds-DNA     circular SYN 03-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Taipale lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 3, 2020 from SnapGene 5.1.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    586..819
                     /label=RRE
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        1223..1723
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             1735..2130
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=Aspergillus terreus BSD
                     /note="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2190..2307
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central 
                     terminationsequence of HIV-1"
     enhancer        2424..2727
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2728..2931
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     primer_bind     2954..2983
                     /label=Primer 1
     protein_bind    2954..2978
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2978..3277
                     /label=ZIM3 KRAB
     protein_bind    complement(3276..3303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3323..3343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3392..3401
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3398..7501
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7505..7531
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA (human influenza hemagglutinin) epitope tag
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             7550..7570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             7577..7597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large 
                     Tantigen"
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     primer_bind     complement(7582..7603)
                     /label=Primer 2
     misc_feature    7645..8233
                     /label=WPRE
                     /note="WPRE"
                     /note="woodchuck hepatitis virus 
                     posttranscriptionalregulatory element"
     LTR             8305..8538
                     /label=3' LTR (Delta-U3)
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8616..8737
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8777..8912
                     /label=SV40 ori
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8933..8951)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8961..8977)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9119..9574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
     promoter        9600..9704
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             9705..10565
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10736..11324
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11612..11633
                     /label=E. coli catabolite activator protein binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
     promoter        11648..11678
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11686..11702
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11710..11726
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11747..11765
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11791..12019
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        11793..12019
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12020..12200
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(12247..12279,1..93)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12247..12279
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virustype
                     1"
ORIGIN
        1 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
       61 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      121 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      181 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      241 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
      301 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
      361 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
      421 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
      481 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
      541 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
      601 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
      661 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
      721 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
      781 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
      841 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
      901 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
      961 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1021 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1081 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1141 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1201 cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     1261 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     1321 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     1381 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     1441 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     1501 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     1561 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     1621 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     1681 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatggcc
     1741 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     1801 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     1861 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
     1921 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     1981 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
     2041 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     2101 ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga attctagatc
     2161 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2221 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2281 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     2341 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     2401 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2461 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2521 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2581 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2641 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2701 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2761 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2821 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2881 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     2941 actgtcggga tcaacaagtt tgtacaaaaa agttggcatg aacaattccc agggaagagt
     3001 gaccttcgag gatgtcactg tgaacttcac ccagggggag tggcagcggc tgaatcccga
     3061 acagagaaac ttgtacaggg atgtgatgct ggagaattac agcaaccttg tctctgtggg
     3121 acaaggggaa accaccaaac ccgatgtgat cttgaggttg gaacaaggaa aggagccatg
     3181 gttggaggaa gaggaagtgc tgggaagtgg ccgtgcagaa aaaaatgggg acattggagg
     3241 gcagatttgg aagccaaagg atgtgaaaga gagtctctac ccaactttct tgtacaaagt
     3301 ggttggtgga tctggaggtt ctcccaagaa gaagcgcaag gtgggacgcg tctgcaggat
     3361 atcaagcttg cggtaccgcg ggcccgggat cgccaccatg gacaagaagt acagcatcgg
     3421 cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     3481 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     3541 cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga agagaaccgc
     3601 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     3661 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     3721 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     3781 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     3841 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     3901 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     3961 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     4021 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     4081 gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga gcctgggcct
     4141 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     4201 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     4261 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     4321 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     4381 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     4441 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg gcggagccag
     4501 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     4561 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     4621 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     4681 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     4741 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     4801 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgccagcgc
     4861 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     4921 gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaagtgaa
     4981 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaagccat
     5041 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     5101 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     5161 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     5221 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     5281 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     5341 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     5401 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     5461 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     5521 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     5581 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     5641 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     5701 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     5761 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     5821 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     5881 ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga gctttctgaa
     5941 ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg gcaagagcga
     6001 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc agctgctgaa
     6061 tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga gaggcggcct
     6121 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     6181 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaacgacaa
     6241 actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     6301 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     6361 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     6421 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     6481 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     6541 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     6601 aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga aagtgctgtc
     6661 tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     6721 gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg actgggaccc
     6781 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     6841 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     6901 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     6961 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     7021 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     7081 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     7141 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact acctggacga
     7201 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     7261 caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc aggccgagaa
     7321 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     7381 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     7441 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     7501 cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc ccaagaaaaa
     7561 acgcaaggtg gaagatccta agaaaaagcg gaaagtggac ggctagtaat gagctagcgc
     7621 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8041 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8221 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8341 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8401 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8461 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8521 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     8581 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8761 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8821 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8881 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8941 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     9001 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     9061 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     9121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9541 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     9601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11521 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    11581 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    11641 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11701 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    11761 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
    11821 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
    11881 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
    11941 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
    12001 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
    12061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    12121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    12181 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    12241 gaggagctct ctcgacgcag gactcggctt gctgaagcg
//
