LOCUS       Exported               14812 bp ds-DNA     circular SYN 03-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Taipale lab
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 3, 2020 from SnapGene 5.1.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    36..624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(627..643)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             734..967
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(992..1010)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1798..1902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1903..2763
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2934..3522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3768..4097
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3948..4083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5231..5296
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5426..5446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5871..6005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6173..6806
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6853..6978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7475..7708
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7893..7937
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8222..8339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9081..9488
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      9557..9566
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9563..13666
                     /codon_start=1
                     /label=dCas9
                     /note="dCas9"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13670..13696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13715..13735
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13742..13762
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13802..14506
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELY"
     misc_feature    14522..14809
                     /label=ZIM3 KRAB
ORIGIN
        1 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
       61 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
      481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      601 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc
      661 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
      721 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
      781 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      961 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     1021 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     1081 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     1141 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     1201 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     1261 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     1321 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     1381 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     1441 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     1501 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1561 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1621 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1681 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1741 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1921 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1981 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2041 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2101 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2161 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2221 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2281 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2341 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2401 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2461 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2521 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2581 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2641 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2701 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2761 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2821 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     3781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4021 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4081 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     4141 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     4201 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     4261 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     4321 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     4381 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     4441 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     4501 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     4561 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     4621 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     4681 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     4741 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     4801 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     4861 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     4921 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     4981 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     5041 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     5101 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     5161 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     5221 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     5281 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     5341 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     5401 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     5461 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     5521 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     5581 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     5641 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     5701 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     5761 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     5821 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6001 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     6061 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     6121 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     6181 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     6241 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     6301 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     6361 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     6421 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     6481 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     6541 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     6601 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     6661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     6841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     6901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     6961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7321 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7381 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7441 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7501 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7561 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7621 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7681 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7741 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7801 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7861 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7921 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7981 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8041 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8101 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     8161 gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca
     8221 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     8281 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     8341 gggtttatta cagggacagc agagatccag tttggatcga taagcttgat atcgaattcg
     8401 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
     8461 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
     8521 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
     8581 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
     8641 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
     8701 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
     8761 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
     8821 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
     8881 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggccccg cgcgcagctc
     8941 ccggctccct cccccttcgg atgtggcttg agctgtaggc gcggagtcct gcagccccga
     9001 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     9061 ggtttggcaa gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa
     9121 gaatagagaa gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag
     9181 gatatctgcg gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag
     9241 tttcggcccc ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca
     9301 gtttcttaag acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc
     9361 cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg
     9421 agctctataa aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt
     9481 cgcccggggg ggatctggag ctctcgagaa ttctcacgcg tctgcaggat atcaagcttg
     9541 cggtaccgcg ggcccggcca ccatggacaa gaagtacagc atcggcctgg ccatcggcac
     9601 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     9661 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggcg ccctgctgtt
     9721 cgacagcgga gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     9781 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     9841 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     9901 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     9961 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
    10021 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
    10081 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
    10141 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
    10201 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
    10261 gaagaatggc ctgttcggca acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
    10321 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
    10381 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
    10441 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
    10501 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
    10561 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
    10621 ccagagcaag aacggctacg ccggctacat cgatggcgga gccagccagg aagagttcta
    10681 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
    10741 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
    10801 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
    10861 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
    10921 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
    10981 caccccctgg aacttcgagg aagtggtgga caagggcgcc agcgcccaga gcttcatcga
    11041 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
    11101 gctgtacgag tacttcaccg tgtacaacga gctgaccaaa gtgaaatacg tgaccgaggg
    11161 aatgagaaag cccgccttcc tgagcggcga gcagaaaaaa gccatcgtgg acctgctgtt
    11221 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
    11281 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
    11341 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
    11401 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
    11461 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
    11521 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
    11581 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
    11641 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
    11701 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
    11761 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
    11821 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
    11881 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
    11941 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
    12001 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
    12061 gtccgactac gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga
    12121 taacaaagtg ctgactcgga gcgacaagaa ccggggcaag agcgacaacg tgccctccga
    12181 agaggtcgtg aagaagatga agaactactg gcgccagctg ctgaatgcca agctgattac
    12241 ccagaggaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
    12301 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
    12361 gatcctggac tcccggatga acactaagta cgacgagaac gacaaactga tccgggaagt
    12421 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
    12481 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
    12541 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
    12601 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
    12661 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
    12721 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaacag gcgagatcgt
    12781 gtgggataag ggccgggact ttgccaccgt gcggaaagtg ctgtctatgc cccaagtgaa
    12841 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
    12901 gaggaacagc gacaagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
    12961 cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
    13021 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
    13081 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
    13141 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
    13201 gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
    13261 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
    13321 gcagaaacag ctgtttgtgg aacagcacaa acactacctg gacgagatca tcgagcagat
    13381 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaagg tgctgagcgc
    13441 ctacaacaag cacagagaca agcctatcag agagcaggcc gagaatatca tccacctgtt
    13501 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg
    13561 gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
    13621 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacgcct atccctatga
    13681 cgtgcccgat tatgccagcc tgggcagcgg ctcccccaag aaaaaacgca aggtggaaga
    13741 tcctaagaaa aagcggaaag tggacggcat tggtagtggg agcaacggca gcagcggatc
    13801 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
    13861 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
    13921 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
    13981 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
    14041 ccccgccgac attcccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
    14101 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
    14161 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
    14221 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
    14281 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
    14341 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
    14401 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
    14461 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacggcg gtggcggagg
    14521 gatgggaaga gtgaccttcg aggatgtcac tgtgaacttc acccaggggg agtggcagcg
    14581 gctgaatccc gaacagagaa acttgtacag ggatgtgatg ctggagaatt acagcaacct
    14641 tgtctctgtg ggacaagggg aaaccaccaa acccgatgtg atcttgaggt tggaacaagg
    14701 aaaggagcca tggttggagg aagaggaagt gctgggaagt ggccgtgcag aaaaaaatgg
    14761 ggacattgga gggcagattt ggaagccaaa ggatgtgaaa gagagtctct ag
//
