LOCUS       pEPYC0CM0382            2211 bp ds-DNA     circular     13-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    158..164
                     /label="TATA BOX"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(481..1069)
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    109..132
                     /label="CRE_MADS+2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    133..156
                     /label="CRE_MADS+2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(1367..2026)
                     /label="CmR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    66..211
                     /label="MinSyn_019"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    85..108
                     /label="CRE_MADS+2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      1252..1346
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(238..258)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          join(2192..2211,1..3)
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TTCAGATAAA AAAAATCCTT AGCTTTCGCT AAGGATGATT TCTGGAATTC GGTCTCAGGA
       61 GGGAGGCGTG TCGTTTTAGT GAGGATCCTT ACCGCTATGG GTAAGATTAT CCTTACCGCT
      121 ATGGGTAAGA TTATCCTTAC CGCTATGGGT AAGATTCTAT ATAAGGTTTT GCTATTCATT
      181 GAAAGCAGTA GTGACTGATT TGTATATAAT GTACTCGAGA CCCTGCAGTC CGGCAAAAAA
      241 GGGCAAGGTG TCACCACCCT GCCCTTTTTC TTTAAAACCG AAAAGATTAC TTCGCGTTAT
      301 GCAGGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
      361 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
      421 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
      481 TTTCCACAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
      541 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      601 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      661 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      721 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
      781 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
      841 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
      901 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
      961 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1021 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     1081 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     1141 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     1201 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC TCGAGGCTTG GATTCTCACC
     1261 AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT
     1321 CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT
     1381 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC
     1441 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT
     1501 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA
     1561 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT
     1621 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA
     1681 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT
     1741 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG
     1801 CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT
     1861 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG
     1921 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC
     1981 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG
     2041 CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT
     2101 GAAAGTTGGA ACCTCTTACG TGCCCGATCA ACTCGAGTGC CACCTGACGT CTAAGAAACC
     2161 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCAGAA T
//