LOCUS       pEPYC0CM0176            2567 bp ds-DNA     circular     28-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(1642..2439)
                     /label="KanR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     292..309
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     motif           507..528
                     /label="NOS_C-CRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           550..560
                     /label="CRE_C2H2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(865..1547)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    406..431
                     /label="CRE_AP2+ II"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     319..339
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(827..1494)
                     /label="Col\E1\origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     motif           564..577
                     /label="CRE_G2like+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGGCCGCT ACAGGGCGCT CCCATTCGCC ATTCAGGCTG CGCAACTGTT
      181 GGGAAGGGCG TTTCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT
      241 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG
      301 ACGGCCAGTG AGCGCGACGT AATACGACTC ACTATAGGGC GAATTGAAGG AAGGCCGTCA
      361 AGGCCGCATT CGGTCTCAGG AGAGCGGAGA ATTAAGGGAG TCACGTTATG ACCCCCGCCG
      421 ATGACGCGGG ACAAGCCGTT TTACGTTTGG AACTGACAGA ACCGCAACGT TGAAGGAGCC
      481 ACTCAGCCGC GGGTTTCTGG AGTTTAATGA GCTAAGCACA TACGTCAGAC GTTCAAAAGT
      541 CGCCTAAGGT CACTATCAGC TAGCAAATAT TTCTTGTCAA AAATGCTCCA CTGACGTTCC
      601 ATAAATTCCC CTCGGTATCC AATTAGAGTC TCATATTCAC TCTCATACTC GAGACCCTCT
      661 GGGCCTCATG GGCCTTCCTT TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
      721 TGCATTAACA TGGTCATAGC TGTTTCCTTG CGTATTGGGC GCTCTCCGCT TCCTCGCTCA
      781 CTGACTCGCT GCGCTCGGTC GTTCGGGTAA AGCCTGGGGT GCCTAATGAG CAAAAGGCCA
      841 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
      901 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
      961 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1021 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1081 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1141 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1201 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1261 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1321 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1381 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1441 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     1501 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     1561 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     1621 TGAGTAAACT TGGTCTGACA GTTATTAGAA AAATTCATCC AGCAGACGAT AAAACGCAAT
     1681 ACGCTGGCTA TCCGGTGCCG CAATGCCATA CAGCACCAGA AAACGATCCG CCCATTCGCC
     1741 GCCCAGTTCT TCCGCAATAT CACGGGTGGC CAGCGCAATA TCCTGATAAC GATCCGCCAC
     1801 GCCCAGACGG CCGCAATCAA TAAAGCCGCT AAAACGGCCA TTTTCCACCA TAATGTTCGG
     1861 CAGGCACGCA TCACCATGGG TCACCACCAG ATCTTCGCCA TCCGGCATGC TCGCTTTCAG
     1921 ACGCGCAAAC AGCTCTGCCG GTGCCAGGCC CTGATGTTCT TCATCCAGAT CATCCTGATC
     1981 CACCAGGCCC GCTTCCATAC GGGTACGCGC ACGTTCAATA CGATGTTTCG CCTGATGATC
     2041 AAACGGACAG GTCGCCGGGT CCAGGGTATG CAGACGACGC ATGGCATCCG CCATAATGCT
     2101 CACTTTTTCT GCCGGCGCCA GATGGCTAGA CAGCAGATCC TGACCCGGCA CTTCGCCCAG
     2161 CAGCAGCCAA TCACGGCCCG CTTCGGTCAC CACATCCAGC ACCGCCGCAC ACGGAACACC
     2221 GGTGGTGGCC AGCCAGCTCA GACGCGCCGC TTCATCCTGC AGCTCGTTCA GCGCACCGCT
     2281 CAGATCGGTT TTCACAAACA GCACCGGACG ACCCTGCGCG CTCAGACGAA ACACCGCCGC
     2341 ATCAGAGCAG CCAATGGTCT GCTGCGCCCA ATCATAGCCA AACAGACGTT CCACCCACGC
     2401 TGCCGGGCTA CCCGCATGCA GGCCATCCTG TTCAATCATA CTCTTCCTTT TTCAATATTA
     2461 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2521 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCAC
//