LOCUS       pEPYCα1KN0003           8814 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5652..5655
                     /label="GTCA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3358..3381
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(6754..7754)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             5344..5427
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3562..3610
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5668..5692
                     /label="RB"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3688..5343
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3118..3135
                     /label="pDGB3 alpha FP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3539..3562
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(943..1203)
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3299..3322
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3327..3435
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(1918..2730)
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3449..3557
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      5432..5647
                     /label="g7 terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3417..3440
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(join(8347..8814,1..533))
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(5681..5698)
                     /label="pDGB3 alpha RP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3209..3317
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3240..3263
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1343..1623)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3163..3188
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3480..3503
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
       61 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
      121 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
      181 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
      241 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
      301 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
      361 aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
      421 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
      481 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
      541 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
      601 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
      661 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
      721 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
      781 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc
      841 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      901 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
      961 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1021 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     1081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtgattagaa
     1921 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     1981 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     2041 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2101 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     2161 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     2221 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     2281 agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     2341 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     2401 taatacctgg aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg
     2461 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     2521 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2581 tggcgcatcg ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc
     2641 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga
     2701 gcaagacgtt tcccgttgaa tatggctcat aacagaactt attatttcct tcctcttttc
     2761 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
     2821 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
     2881 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
     2941 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
     3001 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
     3061 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
     3121 ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg
     3181 tgtaaacata acgaattcgt ctcaggagcg gtgcgggtgc cagggcgtgc ccttgggctc
     3241 cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc
     3301 ccgggcgcgt actccactcc ccTGACcggt gcgggtgcca gggcgtgccc ttgggctccc
     3361 cgggcgcgta ctccactccc cgagccggtg cgggtgccag ggcgtgccct tgggctcccc
     3421 gggcgcgtac tccactcccc CCATggtgcg gtgcgggtgc cagggcgtgc ccttgggctc
     3481 cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc
     3541 ccgggcgcgt actccactcc ccttcgcaag acccttcctc tatataagga agttcatttc
     3601 atttggagag gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa
     3661 cattacaatt tactattcta gtcgaaAATG gaGgacgcca aaaacataaa gaaaggcccg
     3721 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
     3781 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
     3841 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     3901 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     3961 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     4021 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
     4081 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
     4141 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     4201 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
     4261 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
     4321 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
     4381 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     4441 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagaA
     4501 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
     4561 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     4621 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
     4681 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
     4741 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     4801 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     4861 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     4921 attgacaagg atggatggct acattctgga gacatagctt actgggacga Ggacgaacac
     4981 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     5041 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     5101 ctGcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     5161 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     5221 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     5281 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgggT
     5341 TCGGATTATA AGGACCATGA CGGAGACTAT AAGGACCATG ACCTCGACGC TGCAGCAGCG
     5401 GATTATAAGG ACGATGACGA TAAGTGAGCT TGATCCCCCG TCGACAGCTA GCTATATCAT
     5461 CAATTTATGT ATTACACATA ATATCGCACT CAGTCTTTCA TCTACGGCAA TGTACCAGCT
     5521 GATATAATCA GTTATTGAAA TATTTCTGAA TTTAAACTTG CATCAATAAA TTTATGTTTT
     5581 TGCTTGGACT ATAATACCTG ACTTGTTATT TTATCAATAA ATATTTAAAC TATATTTCTT
     5641 TCAAGATCGC Tgtcatgaga cgaattctga caggatatat tggcgggtaa acctaagaga
     5701 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     5761 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     5821 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
     5881 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     5941 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     6001 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     6061 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     6121 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     6181 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     6241 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     6301 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     6361 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     6421 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     6481 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     6541 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     6601 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     6661 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     6721 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     6781 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     6841 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     6901 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatccga
     6961 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     7021 aaggcgggtc aggcaagacg accatcggaa cccatctagc ccgcgccctg caactcgccg
     7081 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     7141 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     7201 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     7261 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     7321 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     7381 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     7441 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     7501 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     7561 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     7621 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     7681 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     7741 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     7801 ccaaggcaag accattaccg agctgctatc tgaatagatc gcgcagctac cagagtaaat
     7861 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     7921 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     7981 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     8041 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     8101 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     8161 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     8221 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     8281 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     8341 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     8401 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     8461 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     8521 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     8581 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     8641 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     8701 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     8761 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaa
//