LOCUS       pEPYCα2rKN0010          7334 bp ds-DNA     circular     10-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6159..6419
                     /label="pBR322"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      5739..6019
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3286..3794
                     /label="NanoLuc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1088..2088
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(4174..4199)
                     /label="LB"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3213..3231
                     /label="Xa27TALE recognition"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             3799..3882
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(3150..3174)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      3887..4149
                     /label="nost"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    join(6829..7334,1..495)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4632..5444
                     /label="KanR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
       61 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
      121 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
      181 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
      241 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
      301 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
      361 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
      421 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
      481 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
      541 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
      601 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
      661 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
      721 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
      781 gcgaccgtca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
      841 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
      901 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
      961 tactcattta ttcatttgct catttactct ggtagctgcg cgatctattc agatagcagc
     1021 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     1081 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     1141 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     1201 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     1261 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     1321 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     1381 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     1441 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     1501 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     1561 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     1621 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     1681 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     1741 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttccgatgg
     1801 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     1861 gcgtatttgt ttatttactc atcggatcat atacgcagcg accgcatgac gcaagctgtt
     1921 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     1981 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     2041 ggttgagagg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     2101 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     2161 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     2221 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     2281 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     2341 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     2401 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     2461 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     2521 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     2581 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     2641 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     2701 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     2761 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     2821 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     2881 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     2941 taggacttgt gcgacatgtc gttttcagat gacggctgca ctgaacgtca gaagccgact
     3001 gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt
     3061 tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga
     3121 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcagatatc
     3181 gtctcaagcg gagGTTCTGT GGGTTTTTTG GGTAGAAGAA GAAACCCATA GCTATATAAT
     3241 GAGAAACAGG GGCAAATCCC CGAAAACAAA TAACGCGAAG CAATGGTATT CACACTCGAA
     3301 GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC TGGACCAAGT CCTTGAACAG
     3361 GGAGGTGTGT CCAGTTTGTT TCAGAATCTC GGGGTGTCCG TAACTCCGAT CCAAAGGATT
     3421 GTCCTGAGCG GTGAAAATGG GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT
     3481 CTGAGCGGCG ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT
     3541 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG GGTTACGCCG
     3601 AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG CCGTGTTCGA CGGCAAAAAG
     3661 ATCACTGTAA CAGGGACCCT GTGGAACGGC AACAAAATTA TCGACGAGCG CCTGATCAAC
     3721 CCCGACGGCT CCCTGCTGTT CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC
     3781 GAACGCATTC TGGCTTCGGA TTATAAGGAC CATGACGGAG ACTATAAGGA CCATGACCTC
     3841 GACGCTGCAG CAGCGGATTA TAAGGACGAT GACGATAAGT GAGCTTGTCA AGCAGATCGT
     3901 TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG TTGCCGGTCT TGCGATGATT
     3961 ATCATATAAT TTCTGTTGAA TTACGTTAAG CATGTAATAA TTAACATGTA ATGCATGACG
     4021 TTATTTATGA GATGGGTTTT TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA
     4081 GAAAACAAAA TATAGCGCGC AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA
     4141 CTAGATCGAC GCTtgactga gacgatatcg ttatgtttac accacaatat atcctgccac
     4201 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     4261 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     4321 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     4381 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg
     4441 cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
     4501 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
     4561 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     4621 taagttctgt tatgagccat attcaacggg aaacgtcttg ctcaaggccg cgattaaatt
     4681 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     4741 gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     4801 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     4861 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     4921 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     4981 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     5041 gtaattgtcc ttttaacagc gatcgcgtat ttcgactcgc tcaggcgcaa tcacgaatga
     5101 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     5161 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     5221 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     5281 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     5341 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     5401 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca cctagaatgc
     5461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5641 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6301 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6361 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     6421 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtcaccggga
     6541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     6601 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     6661 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     6721 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     6781 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     6841 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     6901 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     6961 gggttcccaa tgtacgtgct atccacagga aagagtcctt ttcgaccttt ttcccctgct
     7021 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     7081 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     7141 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     7201 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     7261 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     7321 aagtaatcgg ggtg
//