LOCUS       pEPKKα2KN0102_6X_Op     7676 bp ds-DNA     circular     02-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    429..452
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(5904..6184)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    95..98
                     /label="GGAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    370..393
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    581..1089
                     /label="NanoLuc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      1182..1397
                     /label="g7 terminator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    130..153
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4094..5094)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    48..73
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    339..447
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    248..271
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(5504..5764)
                     /label="pBR322"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    189..212
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Misc._Feature   1178..1181
                     /label="GCTT 4bp overhang"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    452..500
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Golden_Gate_Par 1090..1177
                     /label="CT_FLAGtag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(6479..7291)
                     /label="KanR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    99..207
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2501..3501)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    217..325
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1094..1177
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    307..330
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1415..1439
                     /label="RB"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
       61 tgtggtgtaa acataacaag cttcgtctca gtcaGGAGcg gtgcgggtgc cagggcgtgc
      121 ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc
      181 cttgggctcc ccgggcgcgt actccactcc ccTGACcggt gcgggtgcca gggcgtgccc
      241 ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag ggcgtgccct
      301 tgggctcccc gggcgcgtac tccactcccc CCATggtgcg gtgcgggtgc cagggcgtgc
      361 ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc agggcgtgcc
      421 cttgggctcc ccgggcgcgt actccactcc ccttcgcaag acccttcctc tatataagga
      481 agttcatttc atttggagag gactccggta tttttacaac aattaccaca acaaaacaaa
      541 caaCAAACAA CATTACAATT TACTattcta gtcgaaAATG GTATTCACAC TCGAAGATTT
      601 CGTTGGGGAC TGGCGACAGA CAGCCGGCTA CAACCTGGAC CAAGTCCTTG AACAGGGAGG
      661 TGTGTCCAGT TTGTTTCAGA ATCTCGGGGT GTCCGTAACT CCGATCCAAA GGATTGTCCT
      721 GAGCGGTGAA AATGGGCTGA AGATCGACAT CCATGTCATC ATCCCGTATG AAGGTCTGAG
      781 CGGCGACCAA ATGGGCCAGA TCGAAAAAAT TTTTAAGGTG GTGTACCCTG TGGATGATCA
      841 TCACTTTAAG GTGATCCTGC ACTATGGCAC ACTGGTAATC GACGGGGTTA CGCCGAACAT
      901 GATCGACTAT TTCGGACGGC CGTATGAAGG CATCGCCGTG TTCGACGGCA AAAAGATCAC
      961 TGTAACAGGG ACCCTGTGGA ACGGCAACAA AATTATCGAC GAGCGCCTGA TCAACCCCGA
     1021 CGGCTCCCTG CTGTTCCGAG TAACCATCAA CGGAGTGACC GGCTGGCGGC TGTGCGAACG
     1081 CATTCTGGCT TCGGATTATA AGGACCATGA CGGAGACTAT AAGGACCATG ACCTCGACGC
     1141 TGCAGCAGCG GATTATAAGG ACGATGACGA TAAGTGAGCT TGATCCCCCG TCGACAGCTA
     1201 GCTATATCAT CAATTTATGT ATTACACATA ATATCGCACT CAGTCTTTCA TCTACGGCAA
     1261 TGTACCAGCT GATATAATCA GTTATTGAAA TATTTCTGAA TTTAAACTTG CATCAATAAA
     1321 TTTATGTTTT TGCTTGGACT ATAATACCTG ACTTGTTATT TTATCAATAA ATATTTAAAC
     1381 TATATTTCTT TCAAGATcgc ttgagacgaa gctttgacag gatatattgg cgggtaaacc
     1441 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     1501 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     1561 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat
     1621 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     1681 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     1741 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     1801 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     1861 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     1921 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     1981 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     2041 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     2101 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     2161 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     2221 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     2281 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     2341 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     2401 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     2461 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     2521 gttcgagccg cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     2581 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     2641 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     2701 gatccgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     2761 aaccagaaag gcgggtcagg caagacgacc atcggaaccc atctagcccg cgccctgcaa
     2821 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     2881 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     2941 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     3001 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     3061 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     3121 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     3181 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     3241 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     3301 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     3361 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     3421 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     3481 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     3541 cggtacgcca aggcaagacc attaccgagc tgctatctga atagatcgcg cagctaccag
     3601 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     3661 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     3721 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     3781 aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     3841 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     3901 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     3961 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     4021 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     4081 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     4141 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     4201 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     4261 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     4321 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     4381 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     4441 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     4501 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     4561 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     4621 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     4681 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     4741 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     4801 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     4861 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     4921 aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     4981 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     5041 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     5101 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     5161 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     5221 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     5281 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     5341 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag
     5401 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5461 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     5521 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5581 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     5641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6241 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattaga
     6481 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     6541 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     6601 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     6661 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     6721 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     6781 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     6841 gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     6901 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     6961 ctaatacctg gaatgctgtt ttccctggga tcgcagtggt gagtaaccat gcatcatcag
     7021 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     7081 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     7141 ctggcgcatc gggcttccca tacaatcggt agattgtcgc acctgattgc ccgacattat
     7201 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggccttg
     7261 agcaagacgt ttcccgttga atatggctca taacagaact tattatttcc ttcctctttt
     7321 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
     7381 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
     7441 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
     7501 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
     7561 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
     7621 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagt
//