LOCUS       pEPYC1CB0007            7009 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature      4474..4540
                       /label="TMV omega"
                       /ApEinfo_revcolor=#faac61
                       /ApEinfo_fwdcolor=#faac61
     Terminator        6292..7005
                       /label="ocst"
                       /ApEinfo_revcolor=#d59687
                       /ApEinfo_fwdcolor=#d59687
     Misc._feature     complement(28..155)
                       /label="RB short"
                       /ApEinfo_revcolor=#b1ff67
                       /ApEinfo_fwdcolor=#b1ff67
     Misc._feature     4170..4317
                       /label="LB"
                       /ApEinfo_revcolor=#84b0dc
                       /ApEinfo_fwdcolor=#84b0dc
     Origin            1671..2460
                       /label="pUC Ori"
                       /ApEinfo_revcolor=#85dae9
                       /ApEinfo_fwdcolor=#85dae9
     CDS               4542..6197
                       /label="Firefly\Luciferase"
                       /ApEinfo_revcolor=#75c6a9
                       /ApEinfo_fwdcolor=#75c6a9
     origin            complement(3523..4140)
                       /label="OriV"
                       /ApEinfo_revcolor=#b1ff67
                       /ApEinfo_fwdcolor=#b1ff67
     CDS               complement(2468..3325)
                       /label="AmpR"
                       /ApEinfo_revcolor=#f58a5e
                       /ApEinfo_fwdcolor=#f58a5e
     CDS               6198..6287
                       /label="C terminal V5 (simian virus 5) tag"
                       /ApEinfo_revcolor=#85dae9
                       /ApEinfo_fwdcolor=#85dae9
     4 base annotation 6194..6197
                       /label="TTCG"
                       /ApEinfo_revcolor=#85dae9
                       /ApEinfo_fwdcolor=#85dae9
     misc_feature      4416..4422
                       /label="TATA BOX"
                       /ApEinfo_revcolor=#ff9ccd
                       /ApEinfo_fwdcolor=#ff9ccd
     4 base fusion     4541..4544
                       /label="AATG"
                       /ApEinfo_revcolor=#b1ff67
                       /ApEinfo_fwdcolor=#b1ff67
     Origin            complement(179..1660)
                       /label="RK2"
                       /ApEinfo_revcolor=#84b0dc
                       /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGggaggcgt
     4381 gtcgttttag tgaggtcccc gcatagctga acatctatat aaggttttgc tattcattga
     4441 aagcagtagt gactgatttg tatataatgT ACTGTATTTT TACAACAATT ACCAACAACA
     4501 ACAAACAACA AACAACATTA CAATTACTAT TTACAATTAC aatggaGgac gccaaaaaca
     4561 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
     4621 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     4681 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     4741 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     4801 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     4861 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     4921 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     4981 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     5041 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     5101 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     5161 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     5221 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     5281 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     5341 gatttgaaga agaActgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     5401 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     5461 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     5521 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     5581 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     5641 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     5701 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     5761 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     5821 acgaGgacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     5881 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     5941 caggtgtcgc aggtctGccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     6001 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     6061 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     6121 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     6181 agatcgccgt gggTTCGGGT AAGCCAATCC CTAATCCTCT CTTGGGTTTG GATTCTACTG
     6241 GTGGAAAACC TATTCCAAAC CCATTGCTCG GACTCGACTC AACCTAAGCT TGTCCTGCTT
     6301 TAATGAGATA TGCGAGAAGC CTATGATCGC ATGATATTTG CTTTCAATTC TGTTGTGCAC
     6361 GTTGTAAAAA ACCTGAGCAT GTGTAGCTCA GATCCTTACC GCCGGTTTCG GTTCATTCTA
     6421 ATGAATATAT CACCCGTTAC TATCGTATTT TTATGAATAA TATTCTCCGT TCAATTTACT
     6481 GATTGTACCC TACTACTTAT ATGTACAATA TTAAAATGAA AACAATATAT TGTGCTGAAT
     6541 AGGTTTATAG CGACATCTAT GATAGAGCGC CACAATAACA AACAATTGCG TTTTATTATT
     6601 ACAAATCCAA TTTTAAAAAA AGCGGCAGAA CCGGTCAAAC CTAAAAGACT GATTACATAA
     6661 ATCTTATTCA AATTTCAAAA GTGCCCCAGG GGCTAGTATC TACGACACAC CGAGCGGCGA
     6721 ACTAATAACG CTCACTGAAG GGAACTCCGG TTCCCCGCCG GCGCGCATGG GTGAGATTCC
     6781 TTGAAGTTGA GTATTGGCCG TCCGCTCTAC CGAAAGTTAC GGGCACCATT CAACCCGGTC
     6841 CAGCACGGCG GCCGGGTAAC CGACTTGCTG CCCCGAGAAT TATGCAGCAT TTTTTTGGTG
     6901 TATGTGGGCC CCAAATGAAG TGCAGGTCAA ACCTTGACAG TGACGACAAA TCGTTGGGCG
     6961 GGTCCAGGGC GAATTTTGCG ACAACATGTC GAGGCTCAGC AGGACCGCT
//