LOCUS pEPYC1CB0157 7182 bp ds-DNA circular 27-MAY-2020 DEFINITION . FEATURES Location/Qualifiers Terminator 6465..7178 /label="ocst" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Misc._feature 4170..4317 /label="LB" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Origin complement(179..1660) /label="RK2" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc motif 4542..4563 /label="NOS_C-CRE" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e motif 4567..4580 /label="CRE_G2like+" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 6377..6460 /label="C terminal 3xFLAG (FLAG octapeptide) tag" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS complement(2468..3325) /label="AmpR" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4653..4719 /label="TMV omega" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 4721..6376 /label="LucF" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e origin complement(3523..4140) /label="OriV" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 4396..4421 /label="CRE_AP2+ II" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Misc._feature complement(28..155) /label="RB short" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Origin 1671..2460 /label="pUC Ori" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 motif 4511..4521 /label="CRE_C2H2" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e motif 4497..4509 /label="CRE_Homeobox" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4232..4257 /label="new L2-F" /note="sequence: ctggctggtggcaggatatattgtgg" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(5010..5031) /label="LucF-289-R" /note="sequence: ggcgcaactgcaactccgataa" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4236..4259 /label="L1-F" /note="sequence: CTGGTGGCAGGATATATTGTGGTG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(142..167) /label="L1-R" /note="sequence: GAACCCTGTGGTTGGCATGCACATAC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e ORIGIN 1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG 61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA 121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG 181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC 241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT 301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG 361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT 421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA 481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA 541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG 601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA 661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC 721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA 781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA 841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA 901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC 961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG 1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA 1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA 1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG 1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG 1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC 1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT 1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT 1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC 1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC 1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG 1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC 1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA 2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA 3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG 3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA 3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA 3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA 3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC 3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG 3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG 3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG 3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT 4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT 4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG 4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT 4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT 4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG 4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGAGCGGAGA 4381 ATTAAGGGAG TCACGTTATG ACCCCCGCCG ATGACGCGGG ACAAGCCGTT TTACGTTTGG 4441 AACTGACAGA ACCGCAACGT TGAAGGAGCC ACTCAGCCGC GGGTTTCTGG AGTTTAAACC 4501 ATTATTGCGA TCACTATCAG CCGTTCAAAA GTCGCCTAAG GATGAGCTAA GCACATACGT 4561 CAGTAGCAAA TATTTCTTGT CAAAAATGCT CCACTGACGT TCCATAAATT CCCCTCGGTA 4621 TCCAATTAGA GTCTCATATT CACTCTCATA CTGTATTTTT ACAACAATTA CCAACAACAA 4681 CAAACAACAA ACAACATTAC AATTACTATT TACAATTACA ATGgaGgacg ccaaaaacat 4741 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca 4801 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat 4861 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat 4921 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca 4981 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga 5041 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt 5101 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca 5161 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt 5221 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga 5281 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg 5341 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt 5401 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt 5461 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag 5521 atttgaagaa gaActgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct 5581 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc 5641 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt 5701 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc 5761 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc 5821 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag 5881 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc 5941 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga 6001 cgaGgacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta 6061 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc 6121 aggtgtcgca ggtctGcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt 6181 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac 6241 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 6301 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa 6361 gatcgccgtg ggTTCGGATT ATAAGGACCA TGACGGAGAC TATAAGGACC ATGACCTCGA 6421 CGCTGCAGCA GCGGATTATA AGGACGATGA CGATAAGTGA GCTTGTCCTG CTTTAATGAG 6481 ATATGCGAGA AGCCTATGAT CGCATGATAT TTGCTTTCAA TTCTGTTGTG CACGTTGTAA 6541 AAAACCTGAG CATGTGTAGC TCAGATCCTT ACCGCCGGTT TCGGTTCATT CTAATGAATA 6601 TATCACCCGT TACTATCGTA TTTTTATGAA TAATATTCTC CGTTCAATTT ACTGATTGTA 6661 CCCTACTACT TATATGTACA ATATTAAAAT GAAAACAATA TATTGTGCTG AATAGGTTTA 6721 TAGCGACATC TATGATAGAG CGCCACAATA ACAAACAATT GCGTTTTATT ATTACAAATC 6781 CAATTTTAAA AAAAGCGGCA GAACCGGTCA AACCTAAAAG ACTGATTACA TAAATCTTAT 6841 TCAAATTTCA AAAGTGCCCC AGGGGCTAGT ATCTACGACA CACCGAGCGG CGAACTAATA 6901 ACGCTCACTG AAGGGAACTC CGGTTCCCCG CCGGCGCGCA TGGGTGAGAT TCCTTGAAGT 6961 TGAGTATTGG CCGTCCGCTC TACCGAAAGT TACGGGCACC ATTCAACCCG GTCCAGCACG 7021 GCGGCCGGGT AACCGACTTG CTGCCCCGAG AATTATGCAG CATTTTTTTG GTGTATGTGG 7081 GCCCCAAATG AAGTGCAGGT CAAACCTTGA CAGTGACGAC AAATCGTTGG GCGGGTCCAG 7141 GGCGAATTTT GCGACAACAT GTCGAGGCTC AGCAGGACCG CT //