LOCUS       pEPYC1CB0181            7169 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    4640..4706
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Origin          complement(179..1660)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             6364..6447
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             4708..6363
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           4497..4518
                     /label="NOS_C-CRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   4170..4317
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Origin          1671..2460
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     motif           4540..4550
                     /label="CRE_C2H2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          complement(3523..4140)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     motif           4554..4567
                     /label="CRE_G2like+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4396..4421
                     /label="CRE_AP2+ II"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      6452..7165
                     /label="ocst"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGAGCGGAGA
     4381 ATTAAGGGAG TCACGTTATG ACCCCCGCCG ATGACGCGGG ACAAGCCGTT TTACGTTTGG
     4441 AACTGACAGA ACCGCAACGT TGAAGGAGCC ACTCAGCCGC GGGTTTCTGG AGTTTAATGA
     4501 GCTAAGCACA TACGTCAGAC GTTCAAAAGT CGCCTAAGGT CACTATCAGC TAGCAAATAT
     4561 TTCTTGTCAA AAATGCTCCA CTGACGTTCC ATAAATTCCC CTCGGTATCC AATTAGAGTC
     4621 TCATATTCAC TCTCATACTG TATTTTTACA ACAATTACCA ACAACAACAA ACAACAAACA
     4681 ACATTACAAT TACTATTTAC AATTACAATG gaGgacgcca aaaacataaa gaaaggcccg
     4741 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
     4801 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
     4861 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     4921 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     4981 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     5041 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
     5101 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
     5161 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     5221 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
     5281 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
     5341 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
     5401 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     5461 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagaA
     5521 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
     5581 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     5641 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
     5701 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
     5761 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     5821 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     5881 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     5941 attgacaagg atggatggct acattctgga gacatagctt actgggacga Ggacgaacac
     6001 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     6061 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     6121 ctGcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     6181 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     6241 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     6301 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgggT
     6361 TCGGATTATA AGGACCATGA CGGAGACTAT AAGGACCATG ACCTCGACGC TGCAGCAGCG
     6421 GATTATAAGG ACGATGACGA TAAGTGAGCT TGTCCTGCTT TAATGAGATA TGCGAGAAGC
     6481 CTATGATCGC ATGATATTTG CTTTCAATTC TGTTGTGCAC GTTGTAAAAA ACCTGAGCAT
     6541 GTGTAGCTCA GATCCTTACC GCCGGTTTCG GTTCATTCTA ATGAATATAT CACCCGTTAC
     6601 TATCGTATTT TTATGAATAA TATTCTCCGT TCAATTTACT GATTGTACCC TACTACTTAT
     6661 ATGTACAATA TTAAAATGAA AACAATATAT TGTGCTGAAT AGGTTTATAG CGACATCTAT
     6721 GATAGAGCGC CACAATAACA AACAATTGCG TTTTATTATT ACAAATCCAA TTTTAAAAAA
     6781 AGCGGCAGAA CCGGTCAAAC CTAAAAGACT GATTACATAA ATCTTATTCA AATTTCAAAA
     6841 GTGCCCCAGG GGCTAGTATC TACGACACAC CGAGCGGCGA ACTAATAACG CTCACTGAAG
     6901 GGAACTCCGG TTCCCCGCCG GCGCGCATGG GTGAGATTCC TTGAAGTTGA GTATTGGCCG
     6961 TCCGCTCTAC CGAAAGTTAC GGGCACCATT CAACCCGGTC CAGCACGGCG GCCGGGTAAC
     7021 CGACTTGCTG CCCCGAGAAT TATGCAGCAT TTTTTTGGTG TATGTGGGCC CCAAATGAAG
     7081 TGCAGGTCAA ACCTTGACAG TGACGACAAA TCGTTGGGCG GGTCCAGGGC GAATTTTGCG
     7141 ACAACATGTC GAGGCTCAGC AGGACCGCT
//