LOCUS       pEPYC1CB0187            7057 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     motif           4397..4421
                     /label="CRE_AP2+ I"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   4170..4317
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             4596..6251
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     motif           4447..4468
                     /label="CaMV35s_C-CRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             6252..6335
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Origin          1671..2460
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     motif           4422..4435
                     /label="CRE_bZIP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          complement(3523..4140)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     motif           4436..4446
                     /label="CRE_bHLH1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          complement(179..1660)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4528..4594
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      6340..7053
                     /label="ocst"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGGGAGGCGT
     4381 GTCGTTTTAG TGAGGATCTC TCTGCCGACA GTGGTCCCAA AAACCACGTC TACAAAGCAA
     4441 GTGGATCTGA CGTAAGGGAT GACGCACACT ATATAAGGTT TTGCTATTCA TTGAAAGCAG
     4501 TAGTGACTGA TTTGTATATA ATGTACTGTA TTTTTACAAC AATTACCAAC AACAACAAAC
     4561 AACAAACAAC ATTACAATTA CTATTTACAA TTACAATGga Ggacgccaaa aacataaaga
     4621 aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg
     4681 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     4741 tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     4801 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     4861 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     4921 ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt
     4981 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa
     5041 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     5101 catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg
     5161 acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg
     5221 ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca
     5281 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     5341 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     5401 aagaagaAct gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc
     5461 caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     5521 tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca
     5581 agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta
     5641 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     5701 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg
     5761 aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     5821 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaGg
     5881 acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg
     5941 tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg
     6001 tcgcaggtct Gcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     6061 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     6121 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     6181 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg
     6241 ccgtgggTTC GGATTATAAG GACCATGACG GAGACTATAA GGACCATGAC CTCGACGCTG
     6301 CAGCAGCGGA TTATAAGGAC GATGACGATA AGTGAGCTTG TCCTGCTTTA ATGAGATATG
     6361 CGAGAAGCCT ATGATCGCAT GATATTTGCT TTCAATTCTG TTGTGCACGT TGTAAAAAAC
     6421 CTGAGCATGT GTAGCTCAGA TCCTTACCGC CGGTTTCGGT TCATTCTAAT GAATATATCA
     6481 CCCGTTACTA TCGTATTTTT ATGAATAATA TTCTCCGTTC AATTTACTGA TTGTACCCTA
     6541 CTACTTATAT GTACAATATT AAAATGAAAA CAATATATTG TGCTGAATAG GTTTATAGCG
     6601 ACATCTATGA TAGAGCGCCA CAATAACAAA CAATTGCGTT TTATTATTAC AAATCCAATT
     6661 TTAAAAAAAG CGGCAGAACC GGTCAAACCT AAAAGACTGA TTACATAAAT CTTATTCAAA
     6721 TTTCAAAAGT GCCCCAGGGG CTAGTATCTA CGACACACCG AGCGGCGAAC TAATAACGCT
     6781 CACTGAAGGG AACTCCGGTT CCCCGCCGGC GCGCATGGGT GAGATTCCTT GAAGTTGAGT
     6841 ATTGGCCGTC CGCTCTACCG AAAGTTACGG GCACCATTCA ACCCGGTCCA GCACGGCGGC
     6901 CGGGTAACCG ACTTGCTGCC CCGAGAATTA TGCAGCATTT TTTTGGTGTA TGTGGGCCCC
     6961 AAATGAAGTG CAGGTCAAAC CTTGACAGTG ACGACAAATC GTTGGGCGGG TCCAGGGCGA
     7021 ATTTTGCGAC AACATGTCGA GGCTCAGCAG GACCGCT
//