LOCUS       pEPYC1CB0253            7151 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     motif           4406..4436
                     /label="CRE_MYB+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          1671..2460
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             4690..6345
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           4509..4530
                     /label="MMV_C-CRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4622..4688
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      6434..7147
                     /label="ocst"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     Origin          complement(179..1660)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     origin          complement(3523..4140)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     motif           4437..4449
                     /label="CRE_TCP2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     motif           4460..4488
                     /label="CRE_C2C2dof+2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4567..4573
                     /label="TATA BOX"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(1688..2370)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Misc._feature   4170..4317
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             6346..6429
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGggaggcgt
     4381 gtcgttttag tgaggTATCG TCCTTTGAAG ATAAGATAAT AATGTTGAAG ATAAGAGTGG
     4441 GAGCCACCAC TAAAACATTG CTTTGTCAAA AGCTAAAAAA GATGATGCCC GACAGCCAGA
     4501 CGAAGGAAAT GACGTAAGCC ATGACGTCTA ATCCCACAAG tactgttgga ctctgaagaa
     4561 gatacctata taaggttttg ctattcattg aaagcagtag tgactgattt gtatataTAC
     4621 TGTATTTTTA CAACAATTAC CAACAACAAC AAACAACAAA CAACATTACA ATTACTATTT
     4681 ACAATTACAA TGgaGgacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg
     4741 gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     4801 ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc
     4861 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     4921 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     4981 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     5041 atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg
     5101 aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat
     5161 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     5221 gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac
     5281 tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg
     5341 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     5401 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     5461 tgtggatttc gagtcgtctt aatgtataga tttgaagaag aActgtttct gaggagcctt
     5521 caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa
     5581 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct
     5641 cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg
     5701 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     5761 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     5821 accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt
     5881 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     5941 ctacattctg gagacatagc ttactgggac gaGgacgaac acttcttcat cgttgaccgc
     6001 ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc
     6061 ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtctGcccga cgatgacgcc
     6121 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     6181 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     6241 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     6301 atcctcataa aggccaagaa gggcggaaag atcgccgtgg gTTCGGATTA TAAGGACCAT
     6361 GACGGAGACT ATAAGGACCA TGACCTCGAC GCTGCAGCAG CGGATTATAA GGACGATGAC
     6421 GATAAGTGAG CTTGTCCTGC TTTAATGAGA TATGCGAGAA GCCTATGATC GCATGATATT
     6481 TGCTTTCAAT TCTGTTGTGC ACGTTGTAAA AAACCTGAGC ATGTGTAGCT CAGATCCTTA
     6541 CCGCCGGTTT CGGTTCATTC TAATGAATAT ATCACCCGTT ACTATCGTAT TTTTATGAAT
     6601 AATATTCTCC GTTCAATTTA CTGATTGTAC CCTACTACTT ATATGTACAA TATTAAAATG
     6661 AAAACAATAT ATTGTGCTGA ATAGGTTTAT AGCGACATCT ATGATAGAGC GCCACAATAA
     6721 CAAACAATTG CGTTTTATTA TTACAAATCC AATTTTAAAA AAAGCGGCAG AACCGGTCAA
     6781 ACCTAAAAGA CTGATTACAT AAATCTTATT CAAATTTCAA AAGTGCCCCA GGGGCTAGTA
     6841 TCTACGACAC ACCGAGCGGC GAACTAATAA CGCTCACTGA AGGGAACTCC GGTTCCCCGC
     6901 CGGCGCGCAT GGGTGAGATT CCTTGAAGTT GAGTATTGGC CGTCCGCTCT ACCGAAAGTT
     6961 ACGGGCACCA TTCAACCCGG TCCAGCACGG CGGCCGGGTA ACCGACTTGC TGCCCCGAGA
     7021 ATTATGCAGC ATTTTTTTGG TGTATGTGGG CCCCAAATGA AGTGCAGGTC AAACCTTGAC
     7081 AGTGACGACA AATCGTTGGG CGGGTCCAGG GCGAATTTTG CGACAACATG TCGAGGCTCA
     7141 GCAGGACCGC T
//