LOCUS pEPYC1CB0425 7050 bp ds-DNA circular 27-MAY-2020 DEFINITION . FEATURES Location/Qualifiers Origin 1671..2460 /label="pUC Ori" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Misc._feature complement(28..155) /label="RB short" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 origin complement(3523..4140) /label="OriV" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 4589..6244 /label="LucF" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Terminator 6333..7046 /label="ocst" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Misc._feature 4170..4317 /label="LB" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc motif 4418..4439 /label="CaMV35s_C-CRE" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e motif 4396..4417 /label="CaMV35s_C-CRE" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4521..4587 /label="TMV omega" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Origin complement(179..1660) /label="RK2" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS 6245..6328 /label="C terminal 3xFLAG (FLAG octapeptide) tag" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 motif 4440..4461 /label="CaMV35s_C-CRE" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS complement(2468..3325) /label="AmpR" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4463..4469 /label="TATA BOX" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4236..4259 /label="L1-F" /note="sequence: CTGGTGGCAGGATATATTGTGGTG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(4878..4899) /label="LucF-289-R" /note="sequence: ggcgcaactgcaactccgataa" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e ORIGIN 1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG 61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA 121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG 181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC 241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT 301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG 361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT 421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA 481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA 541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG 601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA 661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC 721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA 781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA 841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA 901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC 961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG 1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA 1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA 1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG 1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG 1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC 1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT 1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT 1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC 1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC 1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG 1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC 1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA 2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA 3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG 3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA 3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA 3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA 3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC 3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG 3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG 3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG 3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT 4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT 4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG 4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT 4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT 4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG 4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGggaggcgt 4381 gtcgttttag tgaggCTGAC GTAAGGGATG ACGCACACTG ACGTAAGGGA TGACGCACAC 4441 TGACGTAAGG GATGACGCAC Actatataag gttttgctat tcattgaaag cagtagtgac 4501 tgatttgtat ataatgTACT GTATTTTTAC AACAATTACC AACAACAACA AACAACAAAC 4561 AACATTACAA TTACTATTTA CAATTACAAT GgaGgacgcc aaaaacataa agaaaggccc 4621 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa 4681 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat 4741 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg 4801 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc 4861 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga 4921 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa 4981 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat 5041 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca 5101 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac 5161 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc 5221 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat 5281 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac 5341 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga 5401 Actgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct 5461 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga 5521 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt 5581 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat 5641 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc 5701 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg 5761 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt 5821 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aGgacgaaca 5881 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc 5941 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg 6001 tctGcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa 6061 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa 6121 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga 6181 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtggg 6241 TTCGGATTAT AAGGACCATG ACGGAGACTA TAAGGACCAT GACCTCGACG CTGCAGCAGC 6301 GGATTATAAG GACGATGACG ATAAGTGAGC TTGTCCTGCT TTAATGAGAT ATGCGAGAAG 6361 CCTATGATCG CATGATATTT GCTTTCAATT CTGTTGTGCA CGTTGTAAAA AACCTGAGCA 6421 TGTGTAGCTC AGATCCTTAC CGCCGGTTTC GGTTCATTCT AATGAATATA TCACCCGTTA 6481 CTATCGTATT TTTATGAATA ATATTCTCCG TTCAATTTAC TGATTGTACC CTACTACTTA 6541 TATGTACAAT ATTAAAATGA AAACAATATA TTGTGCTGAA TAGGTTTATA GCGACATCTA 6601 TGATAGAGCG CCACAATAAC AAACAATTGC GTTTTATTAT TACAAATCCA ATTTTAAAAA 6661 AAGCGGCAGA ACCGGTCAAA CCTAAAAGAC TGATTACATA AATCTTATTC AAATTTCAAA 6721 AGTGCCCCAG GGGCTAGTAT CTACGACACA CCGAGCGGCG AACTAATAAC GCTCACTGAA 6781 GGGAACTCCG GTTCCCCGCC GGCGCGCATG GGTGAGATTC CTTGAAGTTG AGTATTGGCC 6841 GTCCGCTCTA CCGAAAGTTA CGGGCACCAT TCAACCCGGT CCAGCACGGC GGCCGGGTAA 6901 CCGACTTGCT GCCCCGAGAA TTATGCAGCA TTTTTTTGGT GTATGTGGGC CCCAAATGAA 6961 GTGCAGGTCA AACCTTGACA GTGACGACAA ATCGTTGGGC GGGTCCAGGG CGAATTTTGC 7021 GACAACATGT CGAGGCTCAG CAGGACCGCT //