LOCUS       pEPYC1CB0446            7068 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     origin          complement(3523..4140)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4452..4479
                     /label="CRE_bHLH+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   4170..4317
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      6351..7064
                     /label="ocst"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             6263..6346
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4539..4605
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4607..6262
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          1671..2460
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4424..4451
                     /label="CRE_bHLH+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4396..4423
                     /label="CRE_bHLH+"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4481..4487
                     /label="TATA BOX"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(179..1660)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGggaggcgt
     4381 gtcgttttag tgaggGCACA CCAGCATGTG TTGATCACCA GCTGCACACC AGCATGTGTT
     4441 GATCACCAGC TGCACACCAG CATGTGTTGA TCACCAGCTc tatataaggt tttgctattc
     4501 attgaaagca gtagtgactg atttgtatat aatgTACTGT ATTTTTACAA CAATTACCAA
     4561 CAACAACAAA CAACAAACAA CATTACAATT ACTATTTACA ATTACAATGg aGgacgccaa
     4621 aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca
     4681 actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc
     4741 acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga
     4801 agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc
     4861 tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc
     4921 gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt
     4981 ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat
     5041 catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta
     5101 cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc
     5161 cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc
     5221 taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc
     5281 tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca
     5341 cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat
     5401 gtatagattt gaagaagaAc tgtttctgag gagccttcag gattacaaga ttcaaagtgc
     5461 gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga
     5521 tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga
     5581 agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac
     5641 tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt
     5701 tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa
     5761 tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc
     5821 ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta
     5881 ctgggacgaG gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa
     5941 aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt
     6001 cgacgcaggt gtcgcaggtc tGcccgacga tgacgccggt gaacttcccg ccgccgttgt
     6061 tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca
     6121 agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg
     6181 tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg
     6241 cggaaagatc gccgtgggTT CGGATTATAA GGACCATGAC GGAGACTATA AGGACCATGA
     6301 CCTCGACGCT GCAGCAGCGG ATTATAAGGA CGATGACGAT AAGTGAGCTT GTCCTGCTTT
     6361 AATGAGATAT GCGAGAAGCC TATGATCGCA TGATATTTGC TTTCAATTCT GTTGTGCACG
     6421 TTGTAAAAAA CCTGAGCATG TGTAGCTCAG ATCCTTACCG CCGGTTTCGG TTCATTCTAA
     6481 TGAATATATC ACCCGTTACT ATCGTATTTT TATGAATAAT ATTCTCCGTT CAATTTACTG
     6541 ATTGTACCCT ACTACTTATA TGTACAATAT TAAAATGAAA ACAATATATT GTGCTGAATA
     6601 GGTTTATAGC GACATCTATG ATAGAGCGCC ACAATAACAA ACAATTGCGT TTTATTATTA
     6661 CAAATCCAAT TTTAAAAAAA GCGGCAGAAC CGGTCAAACC TAAAAGACTG ATTACATAAA
     6721 TCTTATTCAA ATTTCAAAAG TGCCCCAGGG GCTAGTATCT ACGACACACC GAGCGGCGAA
     6781 CTAATAACGC TCACTGAAGG GAACTCCGGT TCCCCGCCGG CGCGCATGGG TGAGATTCCT
     6841 TGAAGTTGAG TATTGGCCGT CCGCTCTACC GAAAGTTACG GGCACCATTC AACCCGGTCC
     6901 AGCACGGCGG CCGGGTAACC GACTTGCTGC CCCGAGAATT ATGCAGCATT TTTTTGGTGT
     6961 ATGTGGGCCC CAAATGAAGT GCAGGTCAAA CCTTGACAGT GACGACAAAT CGTTGGGCGG
     7021 GTCCAGGGCG AATTTTGCGA CAACATGTCG AGGCTCAGCA GGACCGCT
//