LOCUS       pEPYC1CB0514            7027 bp ds-DNA     circular     27-MAY-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     motif           4409..4430
                     /label="NOS_C-CRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             6222..6305
                     /label="C terminal 3xFLAG (FLAG octapeptide) tag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   complement(28..155)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4498..4564
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     origin          complement(3523..4140)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Origin          complement(179..1660)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     motif           4431..4441
                     /label="CRE_C2H2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             4566..6221
                     /label="LucF"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          1671..2460
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     motif           4396..4408
                     /label="CRE_Homeobox"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Misc._feature   4170..4317
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      6310..7023
                     /label="ocst"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4443..4449
                     /label="TATA BOX"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 GCAATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
       61 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
      121 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
      181 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
      241 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
      301 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
      361 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
      421 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
      481 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
      541 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
      601 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
      661 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
      721 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
      781 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
      841 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
      901 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
      961 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     1021 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     1081 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     1141 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     1201 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     1261 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     1321 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     1381 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     1441 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     1501 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     1561 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     1621 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     1681 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     1741 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     1801 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     1861 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     1921 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     1981 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     2041 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     2101 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA
     2161 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     2221 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     2281 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     2341 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     2401 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     2461 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     2521 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     2581 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     2641 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     2701 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     2761 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     2821 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     2881 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     2941 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     3001 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     3061 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     3121 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     3181 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     3241 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     3301 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     3361 AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     3421 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     3481 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     3541 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     3601 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     3661 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     3721 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     3781 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     3841 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     3901 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     3961 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     4021 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     4081 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
     4141 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
     4201 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
     4261 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
     4321 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGggaggcgt
     4381 gtcgttttag tgaggAACCA TTATTGCGAT GAGCTAAGCA CATACGTCAG TCACTATCAG
     4441 Cctatataag gttttgctat tcattgaaag cagtagtgac tgatttgtat ataTACTGTA
     4501 TTTTTACAAC AATTACCAAC AACAACAAAC AACAAACAAC ATTACAATTA CTATTTACAA
     4561 TTACAATGga Ggacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag
     4621 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa
     4681 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa
     4741 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
     4801 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
     4861 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg
     4921 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg
     4981 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc
     5041 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat
     5101 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct
     5161 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat
     5221 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa
     5281 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg
     5341 gatttcgagt cgtcttaatg tatagatttg aagaagaAct gtttctgagg agccttcagg
     5401 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca
     5461 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc
     5521 tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag
     5581 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac
     5641 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg
     5701 ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt
     5761 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac
     5821 attctggaga catagcttac tgggacgaGg acgaacactt cttcatcgtt gaccgcctga
     5881 agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc
     5941 tccaacaccc caacatcttc gacgcaggtg tcgcaggtct Gcccgacgat gacgccggtg
     6001 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg
     6061 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg
     6121 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc
     6181 tcataaaggc caagaagggc ggaaagatcg ccgtgggTTC GGATTATAAG GACCATGACG
     6241 GAGACTATAA GGACCATGAC CTCGACGCTG CAGCAGCGGA TTATAAGGAC GATGACGATA
     6301 AGTGAGCTTG TCCTGCTTTA ATGAGATATG CGAGAAGCCT ATGATCGCAT GATATTTGCT
     6361 TTCAATTCTG TTGTGCACGT TGTAAAAAAC CTGAGCATGT GTAGCTCAGA TCCTTACCGC
     6421 CGGTTTCGGT TCATTCTAAT GAATATATCA CCCGTTACTA TCGTATTTTT ATGAATAATA
     6481 TTCTCCGTTC AATTTACTGA TTGTACCCTA CTACTTATAT GTACAATATT AAAATGAAAA
     6541 CAATATATTG TGCTGAATAG GTTTATAGCG ACATCTATGA TAGAGCGCCA CAATAACAAA
     6601 CAATTGCGTT TTATTATTAC AAATCCAATT TTAAAAAAAG CGGCAGAACC GGTCAAACCT
     6661 AAAAGACTGA TTACATAAAT CTTATTCAAA TTTCAAAAGT GCCCCAGGGG CTAGTATCTA
     6721 CGACACACCG AGCGGCGAAC TAATAACGCT CACTGAAGGG AACTCCGGTT CCCCGCCGGC
     6781 GCGCATGGGT GAGATTCCTT GAAGTTGAGT ATTGGCCGTC CGCTCTACCG AAAGTTACGG
     6841 GCACCATTCA ACCCGGTCCA GCACGGCGGC CGGGTAACCG ACTTGCTGCC CCGAGAATTA
     6901 TGCAGCATTT TTTTGGTGTA TGTGGGCCCC AAATGAAGTG CAGGTCAAAC CTTGACAGTG
     6961 ACGACAAATC GTTGGGCGGG TCCAGGGCGA ATTTTGCGAC AACATGTCGA GGCTCAGCAG
     7021 GACCGCT
//