LOCUS       Exported                3321 bp ds-DNA     circular SYN 27-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 4, 2020 from SnapGene Viewer 5.1.3
            https://www.snapgene.com
COMMENT     >14B Ecoli T7 ColE1-ori LICv1 biobricks His6-tev-yORF AMP
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    30..49
                     /label=14F seq 49 (14series f oligo)
     misc_feature    43..111
                     /label=terminator 1
     misc_feature    142..191
                     /label=terminator 2
     primer_bind     207..225
                     /label=T7
     misc_feature    283..300
                     /label=His6 tag
     primer_bind     310..357
                     /label=HH322
     misc_feature    310..324
                     /label=Lic_v1_For
     CDS             328..576
                     /codon_start=1
                     /label=ORF1
                     /translation="SKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDT
                     TEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQ"
     primer_bind     complement(552..598)
                     /label=HH323
     misc_feature    584..598
                     /label=Lic_v1_rev
     misc_feature    628..668
                     /label=T7 terminator
     misc_feature    complement(910..938)
                     /label=AmpR_promoter
     misc_feature    971..1831
                     /label=B-Lactamase
     misc_feature    complement(1941..2050)
                     /label=RNA1-for
     misc_feature    complement(2489..2498)
                     /label=ColE1 ORI
     misc_feature    complement(2919..3144)
                     /label=ROP/ROM
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatatacc atgggttctt ctcaccatca ccatcaccat
      301 gaaaacctgt acttccaatc caatgcaagc aaaatgtccg atgttaaatg caccagcgtt
      361 gttctgctga gcgttctgca gcagctgcgt gttgaaagca gcagcaaact gtgggcacag
      421 tgtgttcagc tgcataatga tattctgctg gcaaaagata ccaccgaagc ctttgaaaaa
      481 atggttagcc tgctgagtgt gctgctgagc atgcagggtg cagttgatat taacaaactg
      541 tgtgaagaga tgctggataa tcgtgcaacc ctgcagtaat aacattggaa gtggataact
      601 ggctgctgcc accgctgagc atagcataac cccttggggc ctctaaacgg gtcttgaggg
      661 gttttttgct gaaaggagga actatatcaa gtcccgaagt agctgtcaga ctgctggcga
      721 ccgccgcaac tagtggcgtc atgcgtgccg tatggtcttt cgcctgtaga cgccctacaa
      781 gccgtactaa agtggaaaga cccgcaaaag gtatcgcgat cgccctgcag ggaactatat
      841 ccgactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt
      901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcgaaa
      961 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1021 ttgccttcct gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca
     1081 attgggggca cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag
     1141 ttttcgccct gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc
     1201 agtattatcc cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca
     1261 gaatgacttg gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt
     1321 aagagaatta tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct
     1381 gacaaccatc ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt
     1441 aactcgcctt gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga
     1501 caccacaatg cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact
     1561 tactctggct tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc
     1621 actactgcgt tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga
     1681 gcgtggttca cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt
     1741 agttatctac acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga
     1801 aataggggct tcactgatta agcattggta aaccgataca attaaaggct ccttttggag
     1861 cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt
     1921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2701 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     2761 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     2821 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2881 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2941 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     3001 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     3061 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     3121 cgtgtaaggg ggatttctgt tcatggggta atgataccga tgaaacgaga gaggatgctc
     3181 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     3241 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     3301 gttaatacag atgtaggtgt t
//
