LOCUS       Exported                3666 bp ds-DNA     circular SYN 29-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 4, 2020 from SnapGene Viewer 5.1.3
            https://www.snapgene.com
COMMENT     >14B Ecoli T7 ColE1-ori LICv1 biobricks His6-tev-yORF AMP
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    30..49
                     /label=14F seq 49 (14series f oligo)
     misc_feature    43..111
                     /label=terminator 1
     misc_feature    142..191
                     /label=terminator 2
     primer_bind     207..225
                     /label=T7
     misc_feature    283..300
                     /label=His6 tag
     primer_bind     complement(297..327)
                     /label=HH378
     primer_bind     310..354
                     /label=HH324
     misc_feature    310..324
                     /label=Lic_v1_For
     CDS             328..921
                     /codon_start=1
                     /label=ORF1
                     /translation="AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVA
                     KSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALN
                     NIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDAD
                     SKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ"
     primer_bind     complement(406..432)
                     /label=HH380
     primer_bind     complement(408..450)
                     /label=HH392
     primer_bind     complement(430..462)
                     /label=HH396
     primer_bind     448..467
                     /label=HH381
     primer_bind     complement(451..477)
                     /label=HH389
     primer_bind     454..479
                     /label=HH391
     primer_bind     465..492
                     /label=HH395
     primer_bind     complement(467..495)
                     /label=HH390
     primer_bind     complement(475..498)
                     /label=HH387
     primer_bind     481..505
                     /label=HH388
     primer_bind     complement(488..519)
                     /label=HH394
     primer_bind     502..537
                     /label=HH386
     primer_bind     502..532
                     /label=HH385
     primer_bind     523..551
                     /label=HH393
     primer_bind     559..584
                     /label=HH377
     primer_bind     complement(895..943)
                     /label=HH325
     misc_feature    929..943
                     /label=Lic_v1_rev
     misc_feature    973..1013
                     /label=T7 terminator
     misc_feature    complement(1255..1283)
                     /label=AmpR_promoter
     misc_feature    1316..2176
                     /label=B-Lactamase
     misc_feature    complement(2286..2395)
                     /label=RNA1-for
     misc_feature    complement(2834..2843)
                     /label=ColE1 ORI
     misc_feature    complement(3264..3489)
                     /label=ROP/ROM
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatatacc atgggttctt ctcaccatca ccatcaccat
      301 gaaaacctgt acttccaatc caatgcagca attgcaagcg aatttagcag cctgccgagc
      361 tatgcagcat ttgcaaccgc acaagaggca tatgaacagg cagttgccaa tggtgatagc
      421 gaagttgttc tgaaaaaact gaaaaagtca ctgaacgtgg ccaaatccga atttgatcgt
      481 gatgcagcaa tgcagcgtaa actggaaaaa atggcagatc aggcaatgac ccagatgtat
      541 aaacaggcac gtagcgaaga taaacgtgca aaagttacca gcgcaatgca gaccatgctg
      601 tttaccatgc tgcgcaaact ggataatgat gccctgaaca acattatcaa taatgcccgt
      661 gatggttgtg ttccgctgaa cattattccg ctgaccaccg cagcaaaact gatggtggtt
      721 attccggatt acaacaccta taaaaacacc tgtgatggca ccacctttac ctatgcaagc
      781 gcactgtggg aaattcagca ggttgttgat gcagatagca aaattgttca gctgagcgaa
      841 attagcatgg ataatagccc gaatctggca tggcctctga ttgttaccgc actgcgtgca
      901 aatagcgcag ttaaactgca gtaataacat tggaagtgga taactggctg ctgccaccgc
      961 tgagcatagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     1021 gaggaactat atcaagtccc gaagtagctg tcagactgct ggcgaccgcc gcaactagtg
     1081 gcgtcatgcg tgccgtatgg tctttcgcct gtagacgccc tacaagccgt actaaagtgg
     1141 aaagacccgc aaaaggtatc gcgatcgccc tgcagggaac tatatccgac tagtagcccg
     1201 cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa tacattcaaa
     1261 tatgtatccg ctcatgagac aataaccctg ataaatgctt cgaaaaagga agagtatgag
     1321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1381 tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg gggcacgagt
     1441 gggatacata gaactggact tgaatagcgg taaaatcctt gagagttttc gccctgaaga
     1501 gcgttttcca atgatgagca ctttcaaagt tctgctatgt ggagcagtat tatcccgtgt
     1561 agatgcgggg caagagcaac tcggacgacg aatacactat tcgcagaatg acttggttga
     1621 atactcccca gtgacagaaa agcaccttac ggacggaatg acggtaagag aattatgtag
     1681 tgccgccata acgatgagtg ataacactgc ggcgaactta cttctgacaa ccatcggtgg
     1741 accgaaggaa ttaaccgctt ttttgcacaa tatgggagac catgtaactc gccttgaccg
     1801 ttgggaacca gaactgaatg aagccatacc aaacgacgag cgagacacca caatgcctgc
     1861 ggcaatggca acaacattac gcaaactatt aactggcgaa ctacttactc tggcttcacg
     1921 gcaacaatta atagactggc ttgaagcgga taaagttgca ggaccactac tgcgttcggc
     1981 acttcctgct ggctggttta ttgctgataa atctggggca ggagagcgtg gttcacgggg
     2041 tatcattgcc gcacttggac cagatggtaa gccttcccgt atcgtagtta tctacacgac
     2101 gggtagtcag gcaactatgg acgaacgaaa tagacagatt gctgaaatag gggcttcact
     2161 gattaagcat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     2221 ggaccggtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2281 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2341 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2401 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2461 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2521 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2581 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2641 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2701 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2761 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2821 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2881 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2941 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3001 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3061 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3121 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     3181 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3241 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3301 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     3361 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     3421 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     3481 tctgttcatg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     3541 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     3601 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     3661 ggtgtt
//
