LOCUS       pZCS16_(eft-3p::wrmScar 4339 bp ds-DNA     circular     21-FEB-2020
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     primer_bind     complement(3197..3215)
                     /label="pBRforEco"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3283..3387
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     3137..3159
                     /label="pGEX 3'"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     3' UTR          2441..2772
                     /label="tbb-2 3'UTR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2432..2434
                     /label="ajout STOP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(3018..3037)
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      80..668
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1054..1070
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     569..588
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(3606..3625)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     1054..1070
                     /label="M13 rev"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    1030..1046
                     /label="lac operator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1736..2431
                     /label="wrmScarlet"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Promoter        1120..1727
                     /label="pEFT-3 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    956..977
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        992..1022
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             3388..4248
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      901 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      961 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1021 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     1081 ccaagcttgc atgcctgcag gtcgactcta gaggatcccG CACCTTTGGT CTTTTATTGT
     1141 CAACTTCCAT TGGTTCTTCC ATTGTTTCTG TTAAATTAAT GAATTTTTCA TAAAATAAAG
     1201 ACATTATACA ATATAAAAAT GAAGAATTTA TTGAAAATAA ACTGCCAGAG AGAAAAAGTA
     1261 TGCAACACTC CCGCCGAGAG TGTTTGAAAT GGTGTACGGT ACATTTTCGT GCTAGGAGTT
     1321 AGATGTGCAG GCAGCAACGA GAGGGGGAGA GATTTTTTTG GGCCTTGTGA AATTAACGTG
     1381 AGTTTTCTGG TCATCTGACT AATCATGTTG GTTTTTTGTT GGTTTATTTT GTTTTTATCT
     1441 TTGTTTTTAT CCAGATTAGG AAATTTAAAT TTTATGAATT TATAATGAGG TCAAACATTC
     1501 AGTCCCAGCG TTTTTCCTGT TCTCACTGTT TAGTCGAATT TTTATTTTAG GCTTTCAACA
     1561 AATGTTCTAA CTGTCTTATT TGTGACCTCA CTTTTTATAT TTTTTTAATT TTTAAAAATA
     1621 TTAGAAGTTT CTAGGATAAT TTTTTCGACT TTTATTCTCT CTACCGTCCG CACTCTTCTT
     1681 ACTTTTAAAT TAAATTGTTT TTTTTTCAGT TGGGAAACAC TTTGCTCTAG AaaaaATGGT
     1741 CAGCAAGGGA GAGGCAGTTA TCAAGGAGTT CATGCGTTTC AAGGTCCACA TGGAGGGATC
     1801 CATGAACGGA CACGAGTTCG AGATCGAGGG AGAGGGAGAG GGACGTCCAT ACGAGGGAAC
     1861 CCAAACCGCC AAGCTCAAGG TCACCAAGGG AGGACCACTC CCATTCTCCT GGGACATCCT
     1921 CTCCCCACAA TTCATGTACG GATCCCGTGC CTTCACCAAG CACCCAGCCG ACATCCCAGA
     1981 CTACTACAAG CAATCCTTCC CAGAGGGATT CAAGTGGGAG CGTGTCATGA ACTTCGAGGA
     2041 CGGAGGAGCC GTCACCGTCA CCCAAGACAC CTCCCTCGAG GACGGAACCC TCATCTACAA
     2101 GGTCAAGCTC CGTGGAACCA ACTTCCCACC AGACGGACCA GTCATGCAAA AGAAGACCAT
     2161 GGGATGGGAG GCCTCCACCG AGCGTCTCTA CCCAGAGGAC GGAGTCCTCA AGGGAGACAT
     2221 CAAGATGGCC CTCCGTCTCA AGGACGGAGG ACGTTACCTC GCCGACTTCA AGACCACCTA
     2281 CAAGGCCAAG AAGCCAGTCC AAATGCCAGG AGCCTACAAC GTCGACCGTA AGCTCGACAT
     2341 CACCTCCCAC AACGAGGACT ACACCGTCGT CGAGCAATAC GAGCGTTCCG AGGGACGTCA
     2401 CTCCACCGGA GGAATGGACG AGCTCTACAA GTAAACTAGT gataaatgca aaatcctttc
     2461 aagcattccc ttcttctcta tcactcttct ttctttttgt caaaaaattc tctcgctaat
     2521 ttatttgctt ttttaatgtt attattttat gactttttat agtcactgaa aagtttgcat
     2581 ctgagtgaag tgaatgctat caaaatgtga ttctgtctga tgtactttca caatctctct
     2641 tcaattccat tttgaagtgc tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat
     2701 atttgtattg tgttcgttga gtgacccaac aaaaagagga aactttattg tgccgccaag
     2761 aaaaaagtct cacgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     2821 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2881 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2941 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3001 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3061 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3121 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3181 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3241 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4321 gtgaagatcc tttttgata
//