LOCUS       PL0588_CLED             3759 bp ds-DNA     circular     09-JUN-2020
DEFINITION  .
KEYWORDS    "Responsible person/group:darse" "Usage" "Status" "Parent plasmids"
            "Cloning sites:USER" "Antibiotic resistance:Ampicillin" "Origin of
            replication" "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers"
            "gRNAs" "Terminators" "DNA fragments" "Backbones" "Design entry"
            "LabCollector ID" "Comment" "Old
            names:8673;loxP-pDJ-cGCCACC-EPO-lox2272"
FEATURES             Location/Qualifiers
     USER linker     1384..1392
                     /label="LinkB"
     oriT            complement(2187..2775)
                     /label="Ori"
     USER linker     379..387
                     /label="LinkA"
     CDS             complement(2899..3759)
                     /label="AmpR"
     CDS             802..1383
                     /label="coEPO"
     Region          1393..1426
                     /label="Lox2272"
     USER linker     786..794
                     /label="LinkC"
     Region          388..421
                     /label="LoxP"
     USER linker     422..430
                     /label="LinkA"
     Region          join(1435..3759,1..378)
                     /label="Backbone"
     USER linker     1427..1434
                     /label="LinkO5"
     promoter        431..785
                     /label="100RPU.2"
     Kozak           795..801
                     /label="Kozak"
ORIGIN
        1 ATTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
       61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC AGTGTTACAA CCAATTAACC
      121 AATTCTGAAC ATTATCGCGA GCCCATTTAT ACCTGAATAT GGCTCATAAC ACCCCTTGTT
      181 TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC
      241 GCCGTAGCGC CGATGGTAGT GTGGGGACTC CCCATGCGAG AGTAGGGAAC TGCCAGGCAT
      301 CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GCCCGGGCTA ATTATGGGGT
      361 GTCGCCCTTA TTCGACTCAG TCGGTGTATA ACTTCGTATA GCATACATTA TACGAAGTTA
      421 TAGTCGGTGT TGGGACTTTC CACCTTAGAT GACACAGCAA TCAGATTTGC TTGCGTGAGA
      481 AGATATAGGA TGACACAGCA ATCTAGACTG GGACTTTCCA CTGATATTTT GCGCAATTGA
      541 CCTAATGACA CAGCAATAGT ATGTGGGGCG GGGATCTAAC TGGGACTTTC CAAAGGTCTT
      601 ACCGGAAGTT GTTAGAATGA CACAGCAATG GATTCATATC CTGGGACTTT CCAGTATACT
      661 GCTTGCGTGA GAAGATGATC ATGGGACTTT CCATGTACAA AAGGTCTATA TAAGCAGAGC
      721 TCGTTTAGTG AACCGTCAGA TCGCCTAGAT ACGCCATCCA CGCTGTTTTG ACCTCCATAG
      781 AAGACAGACG TCATcGCCAC CATGGGAGTG CACGAGTGTC CTGCTTGGCT GTGGCTGCTG
      841 CTGTCCCTGC TGTCTCTGCC TCTGGGACTG CCTGTGCTGG GCGCTCCTCC TAGACTGATC
      901 TGCGACTCCC GGGTGCTGGA AAGATACCTG CTGGAAGCCA AAGAGGCCGA GAACATCACC
      961 ACCGGCTGCG CCGAGCACTG CTCCCTGAAC GAGAATATCA CCGTGCCCGA CACCAAAGTG
     1021 AACTTCTACG CCTGGAAGCG GATGGAAGTG GGCCAGCAGG CTGTGGAAGT GTGGCAGGGA
     1081 CTGGCTCTGC TGAGCGAGGC TGTGCTGAGA GGACAGGCCC TGCTCGTGAA CTCCTCCCAG
     1141 CCTTGGGAAC CCCTGCAGCT GCACGTGGAC AAGGCTGTGT CCGGCCTGAG ATCCCTGACC
     1201 ACCCTGCTGA GAGCACTGGG AGCCCAGAAA GAGGCCATCT CTCCACCTGA CGCCGCCTCT
     1261 GCTGCTCCTC TGAGAACCAT CACCGCCGAC ACCTTCAGAA AGCTGTTCCG GGTGTACTCC
     1321 AACTTCCTGC GGGGCAAGCT GAAGCTGTAC ACCGGCGAGG CTTGCCGGAC CGGCGATAGA
     1381 TGAAAGCAGC GTATAACTTC GTATAGGATA CTTTATACGA AGTTATACTT GCGTAGTGAG
     1441 TCGAATAAGG GCGACACAAA ATTTATTCTA AATGCATAAT AAATACTGAT AACATCTTAT
     1501 AGTTTGTATT ATATTTTGTA TTATCGTTGA CATGTATAAT TTTGATATCA AAAACTGATT
     1561 TTCCCTTTAT TATTTTCGAG ATTTATTTTC TTAATTCTCT TTAACAAACT AGAAATATTG
     1621 TATATACAAA AAATCATAAA TAATAGATGA ATAGTTTAAT TATAGGTGTT CATCAATCGA
     1681 AAAAGCAACG TATCTTATTT AAAGTGCGTT GCTTTTTTCT CATTTATAAG GTTAAATAAT
     1741 TCTCATATAT CAAGCAAAGT GACAGGCGCC CTTAAATATT CTGACAAATG CTCTTTCCCT
     1801 AAACTCCCCC CATAAAAAAA CCCGCCGAAG CGGGTTTTTA CGTTATTTGC GGATTAACGA
     1861 TTACTCGTTA TCAGAACCGC CCAGGGGGCC CGAGCTTAAG ACTGGCCGTC GTTTTACAAC
     1921 ACAGAAAGAG TTTGTAGAAA CGCAAAAAGG CCATCCGTCA GGGGCCTTCT GCTTAGTTTG
     1981 ATGCCTGGCA GTTCCCTACT CTCGCCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
     2041 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
     2101 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
     2161 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
     2221 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
     2281 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
     2341 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
     2401 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
     2461 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
     2521 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
     2581 GCTACAGAGT TCTTGAAGTG GTGGGCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
     2641 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
     2701 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
     2761 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     2821 GACGCGCGCG TAACTCACGT TAAGGGATTT TGGTCATGAG CTTGCGCCGT CCCGTCAAGT
     2881 CAGCGTAATG CTCTGCTTTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     2941 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     3001 GGGCTTACCA TCTGGCCCCA GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC
     3061 GGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     3121 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     3181 AGTTAATAGT TTGCGCAACG TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC
     3241 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     3301 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     3361 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     3421 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     3481 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     3541 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     3601 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     3661 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     3721 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCAT
//