LOCUS Exported 5593 bp ds-DNA circular SYN 18-JUN-2020 DEFINITION . ACCESSION . VERSION . KEYWORDS pAAV.CMV.CD86.C-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5593) AUTHORS UNKNOWN TITLE Direct Submission JOURNAL Exported Thursday, Jun 18, 2020 from SnapGene Viewer 5.1.3.1 https://www.snapgene.com COMMENT ORIGDB|GenBank FEATURES Location/Qualifiers source 1..5593 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /label=AAV2\ITR /note="inverted terminal repeat of adeno-associated virus serotype 2 vntifkey=34" /note="color: #ffff99" enhancer 221..524 /label=CMV enhancer /label=CMV\enhancer /note="human cytomegalovirus immediate early enhancer vntifkey=9" /note="color: #c0c0c0" promoter 525..727 /label=CMV promoter /label=CMV\promoter /note="human cytomegalovirus (CMV) immediate early promoter vntifkey=30" /note="color: #c0c0c0; direction: RIGHT" intron 820..1312 /label=beta-Globin intron /label=-beta-G\intron /note="vntifkey=15" /note="color: #------" CDS 1373..2299 /codon_start=1 /label=CD86 /label=CD86-C-HA /note="vntifkey=4" /note="color: #ff9900" /translation="MDPRCTMGLAILIFVTVLLISDAVSVETQAYFNGTAYLPCPFTKA QNISLSELVVFWQDQQKLVLYEHYLGTEKLDSVNAKYLGRTSFDRNNWTLRLHNVQIKD MGSYDCFIQKKPPTGSIILQQTLTELSVIANFSEPEIKLDQNVTGNSGINLTCMSKQGH PKPKKMYFLITNSTNEYGDNMQISQDNVTELFSISNSLSLSFPDGVWHMTVVCVLETES MKISSKPLNFTQEFPSAQTYWKEITASVTVALLLVMLLIIVCHKKPNQPSRPSNTASKL ERDSNADRETINLKELEPQIASAKPNAE" CDS 2303..2329 /codon_start=1 /label=HA-tag /label=C-HA-Tag /label=insertion_seq /note="C-terminal HA-tag vntifkey=14" /note="color: #ff0000" /translation="YPYDVPDYA" polyA_signal 2340..2816 /label=hGH poly(A) signal /label=hGH\poly(A)\signal /note="human growth hormone polyadenylation signal vntifkey=25" /note="color: #------" repeat_region 2856..2996 /label=AAV2 ITR /label=AAV2\ITR /note="inverted terminal repeat of adeno-associated virus serotype 2 vntifkey=34" /note="color: #ffff99" rep_origin 3071..3526 /direction=RIGHT /label=f1 ori /label=f1\ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis vntifkey=33" /note="color: #------" promoter 3808..3912 /gene="bla" /label=AmpR promoter /label=AmpR\promoter /note="vntifkey=30" /note="color: #800000; direction: RIGHT" CDS 3913..4773 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics vntifkey=4" /note="color: #ffcc99" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4944..5532 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication vntifkey=33" /note="color: #------" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt ccccggggat cctctagagt cgacctgcag aagctaccca cgatggaccc 1381 cagatgcacc atgggcttgg caatccttat ctttgtgaca gtcttgctga tctcagatgc 1441 tgtttccgtg gagacgcaag cttatttcaa tgggactgca tatctgccgt gcccatttac 1501 aaaggctcaa aacataagcc tgagtgagct ggtagtattt tggcaggacc agcaaaagtt 1561 ggttctgtac gagcactatt tgggcacaga gaaacttgat agtgtgaatg ccaagtacct 1621 gggccgcacg agctttgaca ggaacaactg gactctacga cttcacaatg ttcagatcaa 1681 ggacatgggc tcgtacgatt gttttataca aaaaaagcca cccacaggat caattatcct 1741 ccaacagaca ttaacagagc tgtcagtgat cgccaacttc agtgaacctg aaataaaact 1801 ggatcagaat gtaacaggaa attctggcat aaatttgacc tgcatgtcta agcaaggtca 1861 cccgaaacct aagaagatgt attttctgat aactaattca actaatgagt atggtgataa 1921 catgcagata tcacaagata atgtcacaga actgttcagt atctccaaca gcctctctct 1981 ttcattcccg gatggtgtgt ggcatatgac cgttgtgtgt gttctggaaa cggagtcaat 2041 gaagatttcc tccaaacctc tcaatttcac tcaagagttt ccatctgctc aaacgtattg 2101 gaaggagatt acagcttcag ttactgtggc cctcctcctt gtgatgctgc tcatcattgt 2161 atgtcacaag aagccgaatc agcctagcag gcccagcaac acagcctcta agttagagcg 2221 ggatagtaac gctgacagag agactatcaa cctgaaggaa cttgaacccc aaattgcttc 2281 agcaaaacca aatgcagaag cttacccata cgatgttcca gattacgctt aggatctacg 2341 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag 2401 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct 2461 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa 2521 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc 2581 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt 2641 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 2701 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt 2761 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga 2821 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc 2881 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg 2941 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg 3001 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag 3061 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 3241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca 3301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct 3421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 3481 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc 3541 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 3601 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 3661 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 3721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 3781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 3961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 4021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 4141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 4201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 4261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 4321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 4381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 4441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 4501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 4561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 4621 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 4681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 4741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 4801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5581 tttgctcaca tgt //