LOCUS       Exported                6093 bp ds-DNA     circular SYN 18-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pAAV.CMV.VTN.C-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 18, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     enhancer        221..524
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer
                     vntifkey=9"
                     /note="color: #c0c0c0"
     promoter        525..727
                     /label=CMV promoter
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter
                     vntifkey=30"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          820..1312
                     /label=beta-Globin intron
                     /label=-beta-G\intron
                     /note="vntifkey=15"
                     /note="color: #------"
     CDS             1366..2800
                     /codon_start=1
                     /label=VITRONECTIN
                     /label=Vitronectin-C-HA
                     /note="vntifkey=4"
                     /note="color: #ff9900"
                     /translation="MAPLRPFFILALVAWVSLADQESCKGRCTQGFMASKKCQCDELCT
                     YYQSCCADYMEQCKPQVTRGDVFTMPEDDYWSYDYVEEPKNNTNTGVQPENTSPPGDLN
                     PRTDGTLKPTAFLDPEEQPSTPAPKVEQQEEILRPDTTDQGTPEFPEEELCSGKPFDAF
                     TDLKNGSLFAFRGQYCYELDETAVRPGYPKLIQDVWGIEGPIDAAFTRINCQGKTYLFK
                     GSQYWRFEDGVLDPGYPRNISEGFSGIPDNVDAAFALPAHRYSGRERVYFFKGKQYWEY
                     EFQQQPSQEECEGSSLSAVFEHFALLQRDSWENIFELLFWGRSSDGAREPQFISRNWHG
                     VPGKVDAAMAGRIYVTGSLSHSAQAKKQKSKRRSRKRYRSRRGRGHRRSQSSNSRRSSR
                     SIWFSLFSSEESGLGTYNNYDYDMDWLVPATCEPIQSVYFFSGDKYYRVNLRTRRVDSV
                     NPPYPRSIAQYWLGCPTSEK"
     CDS             2803..2829
                     /codon_start=1
                     /label=HA-tag
                     /label=C-HA-Tag
                     /label=insertion_seq
                     /note="C-terminal HA-tag
                     vntifkey=14"
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     polyA_signal    2840..3316
                     /label=hGH poly(A) signal
                     /label=hGH\poly(A)\signal
                     /note="human growth hormone polyadenylation signal
                     vntifkey=25"
                     /note="color: #------"
     repeat_region   3356..3496
                     /label=AAV2 ITR
                     /label=AAV2\ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2
                     vntifkey=34"
                     /note="color: #ffff99"
     rep_origin      3571..4026
                     /direction=RIGHT
                     /label=f1 ori
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis
                     vntifkey=33"
                     /note="color: #------"
     promoter        4308..4412
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="vntifkey=30"
                     /note="color: #800000; direction: RIGHT"
     CDS             4413..5273
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics
                     vntifkey=4"
                     /note="color: #ffcc99"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5444..6032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication
                     vntifkey=33"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt ccccggggat cctctagagt cgacctgcag aagctatggc acccctgagg
     1381 ccctttttca tactagccct ggtggcatgg gtttctctgg ctgaccaaga gtcatgcaag
     1441 ggccgctgca ctcagggttt catggccagc aagaagtgtc agtgtgacga gctttgcact
     1501 tactatcaga gctgctgtgc cgactacatg gagcagtgca agccccaagt aacgcggggg
     1561 gacgtgttca ctatgccaga ggatgattat tggagctatg actacgtgga ggagcccaag
     1621 aacaatacca acaccggtgt gcaacccgag aacacctctc cacccggtga cctaaatcct
     1681 cggacggacg gcactctaaa gccgacagcc ttcctagatc ctgaggaaca gccaagcacc
     1741 ccagcgccta aagtggagca acaggaggag atcctaaggc ccgacaccac tgatcaaggg
     1801 acccctgagt ttccagagga agaactgtgc agtggaaagc cctttgacgc cttcacggat
     1861 ctcaagaatg ggtccctctt tgccttccga gggcagtact gctatgagct agatgagacg
     1921 gcagtgaggc ctgggtaccc caaacttatc caagatgtct ggggcattga gggccccatc
     1981 gatgctgcct tcactcgcat caactgtcag gggaagacct acttgttcaa gggtagtcag
     2041 tactggcgct ttgaggatgg ggtcctggac cctggttatc cccgaaacat ctccgaaggc
     2101 ttcagtggca taccagacaa tgttgatgca gcgttcgccc ttcctgccca ccgttacagt
     2161 ggccgggaaa gggtctactt cttcaagggg aagcagtact gggagtacga atttcagcag
     2221 caacccagcc aggaggagtg cgaaggcagc tctctgtcag ccgtgtttga gcactttgcc
     2281 ttgcttcagc gggacagctg ggagaacatt ttcgaactcc tcttctgggg cagatcctct
     2341 gatggagcca gagaacccca attcatcagc cggaactggc atggtgtgcc agggaaagtg
     2401 gacgctgcta tggccggccg catctacgtc actggctcct tatcccactc tgcccaagcc
     2461 aaaaaacaga agtctaagcg tagaagccga aagcgctatc gttcacgccg agggcgtggc
     2521 cacagacgca gccagagctc gaactcccgt cgttcatcac gttcaatctg gttctctttg
     2581 ttctccagcg aggagagtgg gctaggaacc tacaacaact atgattatga tatggactgg
     2641 cttgtacctg ccacctgcga gcccattcag agcgtctatt tcttctctgg agacaaatac
     2701 taccgagtca accttagaac ccggcgagtg gactctgtga atcctcccta cccacgctcc
     2761 attgctcagt attggctggg ctgcccgacc tctgagaaag cttacccata cgatgttcca
     2821 gattacgctt aggatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     2881 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     2941 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3001 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3061 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3121 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3181 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3241 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3301 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     3361 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3421 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3481 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3541 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3721 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3781 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3901 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3961 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4021 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4141 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4201 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4261 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5581 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6061 tcctggcctt ttgctggcct tttgctcaca tgt
//
