LOCUS       Exported                7150 bp ds-DNA     circular SYN 15-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 22, 2020 from SnapGene Viewer 5.1.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffff99"
     enhancer        168..296
                     /label=partial CMV enhancer
                     /note="color: #c0c0c0"
     promoter        297..500
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #c0c0c0; direction: RIGHT"
     intron          542..634
                     /label=SV40 intron
                     /note="color: #------"
     primer_bind     601..627
                     /label=Non-secreting-For
                     /note="color: black; sequence: TTTCTCTCTTTTAGATTCCAACCTTTG"
     CDS             685..4197
                     /codon_start=1
                     /label=THBS1
                     /note="color: #ff9900"
                     /translation="MELLRGLGVLFLLHMCGSNRIPESGGDNGVFDIFELIGGARRGPG
                     RRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTL
                     LAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQED
                     RAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTT
                     PEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM
                     VLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTEC
                     HCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNG
                     IQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVIT
                     RIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQRRS
                     RLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGAC
                     PPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRGV
                     EHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDT
                     DLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRD
                     NCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDKNGEGDACAVDIDGDGILNERD
                     NCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDLIGDTCDNNQDIDEDGHQNN
                     LDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDA
                     CKDDFDHDNVPDIDDICPENFDISETDFRQFQMIPLDPKGTSQNDPNWVVRHQGKELVQ
                     TVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSY
                     WDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFT
                     AYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKY
                     ECRDS"
     primer_bind     complement(940..960)
                     /label=Non-secreting-Rev
                     /note="color: black; sequence: AGCCAGGAAGATGAAGCCTTT"
     primer_bind     1000..1021
                     /label=CC-S
                     /note="color: black; sequence: GCTGTGGAACGGAAAGACAACA"
     primer_bind     complement(2010..2029)
                     /label=CC-AS
                     /note="color: black; sequence: AACAGGACGACCATGGAGAC"
     CDS             4198..4224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #ff0000"
                     /translation="YPYDVPDYA"
     intron          4268..4401
                     /label=SV40 polyA
                     /note="color: #------"
     repeat_region   4413..4553
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffff99"
     rep_origin      4628..5083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #------"
     promoter        5365..5469
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #993300; direction: RIGHT"
     CDS             5470..6330
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5470 .. 5538 / #ffcc99 / signal sequence
                        2: 5539 .. 6330 / #ffcc99
                     Cleavage site after base 5538"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6501..7089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #------"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcc gctcgtgacc taggcatatg ccaagtacgc
      181 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      241 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      301 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      361 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      421 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      481 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccgg
      541 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      601 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      661 gtcgacctgc agaagcttat gaccatggag ctcctgcggg gactaggtgt cctgttcctg
      721 ttgcatatgt gtggaagcaa ccgcattcca gagtctgggg gagataacgg tgtgtttgac
      781 atctttgaac tcattggagg tgcacgaagg ggccccggtc gccgactggt gaagggccaa
      841 gatctatcca gccccgcctt ccgcattgag aatgccaacc tgatccccgc tgtgccggat
      901 gacaagttcc aagacctact ggacgctgtg tgggccgaca aaggcttcat cttcctggct
      961 tccttgaggc agatgaagaa gacccggggc acactcctgg ctgtggaacg gaaagacaac
     1021 actggccaga tcttcagtgt ggtctccaac ggcaaagctg gcaccctgga cctgagcctg
     1081 agcctgccag ggaagcaaca agtggtgtca gtggaggaag ctctcctggc cactggccag
     1141 tggaagagca tcacgctgtt tgttcaagag gaccgggctc aactctacat agactgtgat
     1201 aagatggaga gcgcggagct ggatgtaccc atccagagca tcttcaccag ggatctggcc
     1261 agcgttgcca ggctccgagt tgcaaaggga gatgtcaatg acaattttca gggggtgctg
     1321 cagaatgtga ggtttgtctt tggaaccacc ccagaagaca ttctcaggaa caaaggctgc
     1381 tccagctcag ctaccaacgt ccttcttacc cttgacaaca acgtggtgaa cggttccagc
     1441 cctgctatcc gcaccaacta catcggccac aaaacaaagg acctccaagc tatctgtggc
     1501 ctctcctgtg atgaactatc cagcatggtc ctggaactga agggcctgcg caccatcgtg
     1561 accactctgc aggacagcat ccgaaaagtg acggaagaga acagagagct ggtcagtgag
     1621 ctgaagcggc ctcccctctg ctttcacaat ggagtccagt acaagaacaa cgaggagtgg
     1681 actgtagaca gttgcacaga gtgtcactgc cagaactcgg ttaccatctg caaaaaggtg
     1741 tcctgtccca tcatgccctg ctccaacgcc acagttcctg atggtgaatg ctgcccacgg
     1801 tgctggccca gcgactctgc tgacgatggc tggtctccct ggtctgagtg gacctcctgc
     1861 tctgccacat gtggcaatgg aattcagcaa cgtggtcgtt cctgtgacag cctcaacaac
     1921 agatgcgagg gctcttcggt acagacaagg acctgccaca ttcaggagtg tgacaaaaga
     1981 tttaaacagg atggtggctg gagtcactgg tctccatggt cgtcctgttc tgtgacctgt
     2041 ggtgacggtg tgatcacaag gatccggctc tgcaactccc ccagccccca gatgaacggg
     2101 aagccctgtg aaggtgaagc ccgggagacc aaagcctgca agaaagacgc ctgcccaatt
     2161 aatggaggct ggggtccctg gtcaccatgg gacatctgct ctgtcacctg tggaggagga
     2221 gtgcagagac gcagccgact ctgtaacaac cccacacccc agtttggagg caaagactgt
     2281 gttggcgatg tgacagaaaa tcaagtttgc aacaagcagg actgcccaat tgatggatgc
     2341 ctgtccaatc cctgctttgc tggtgccaag tgtactagct accctgatgg tagctggaaa
     2401 tgtggtgcgt gtcctcctgg ctacagtgga aatggcatcc agtgcaaaga cgtcgatgag
     2461 tgcaaagaag tgcctgatgc ttgcttcaat cacaacggag aacatcggtg caagaacaca
     2521 gatcctggct acaactgcct gccctgccca ccacgattca ctggctcaca gcccttcggc
     2581 cgaggtgtcg aacatgccat ggccaacaaa caggtgtgca aaccgcgaaa cccctgcacg
     2641 gacgggacgc atgactgcaa caagaacgct aagtgcaact acctgggtca ctacagtgac
     2701 cccatgtacc gctgtgagtg caagcccggc tatgcaggca atggcatcat ctgcggagag
     2761 gacacagacc tggacggctg gcctaatgaa aacctggtgt gtgtggccaa cgcaacctac
     2821 cactgcaaaa aggacaactg ccccaacctt cccaactcgg ggcaggaaga ctatgacaag
     2881 gacgggattg gcgatgcctg cgatgatgac gatgacaacg acaagatccc cgatgacagg
     2941 gacaactgtc cattccatta caacccagcc cagtatgact atgacagaga tgatgtggga
     3001 gaccgctgtg acaactgccc ctacaaccac aaccctgacc aagcagacac agacaaaaac
     3061 ggggagggcg atgcctgtgc tgtggacatc gatggagatg gaatcctcaa tgaacgagac
     3121 aactgccagt acgtttacaa cgtggaccag agggacacgg acatggatgg ggttggagat
     3181 cagtgtgaca actgccccct ggaacacaat ccagaccagc tggactctga ctcagacctc
     3241 ataggggaca cttgtgacaa caatcaggac atcgatgagg atggccatca gaacaatctg
     3301 gacaactgtc cctatgtgcc taatgccaac caggccgacc atgataaaga tggcaaagga
     3361 gatgcctgtg accatgacga tgacaatgac ggcatccctg atgacagaga caactgcagg
     3421 ctggtgccca atcctgacca gaaggactct gatggtgatg gccgaggtga cgcctgcaaa
     3481 gacgactttg accatgacaa tgtgccagat attgatgaca tctgtcctga gaattttgac
     3541 atcagtgaaa ccgatttccg acaattccag atgattcctc tagatcccaa aggaacctcc
     3601 caaaatgacc ctaactgggt tgtccgccat cagggcaaag aacttgtcca gactgtaaac
     3661 tgtgaccctg gacttgctgt aggttatgat gagtttaatg ctgtggactt cagcggtacc
     3721 ttcttcatca acaccgagag agatgatgac tacgctggct ttgttttcgg ctaccagtcc
     3781 agcagccgct tctacgttgt gatgtggaaa caagtcaccc agtcctactg ggacaccaac
     3841 cccacaaggg ctcagggata ctcaggcctg tctgtaaagg ttgtgaactc caccaccggc
     3901 cctggcgagc acctgcggaa tgcactgtgg cacacaggaa acacccctgg ccaggtgcgc
     3961 accctgtggc atgaccctcg ccacatcggc tggaaagatt tcactgcata cagatggcgt
     4021 ctcagccaca ggccaaagac cggttatatc agagtggtga tgtatgaagg aaagaaaatc
     4081 atggctgact cgggacccat ctatgacaaa acctacgccg gcggtagact aggcctgttc
     4141 gtcttctctc aggaaatggt gttcttctca gacatgaaat acgagtgtcg agattcctac
     4201 ccatacgatg ttccagatta cgcttaactc gagagatcta ggccgctagg cctcacctgc
     4261 gatctcgatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4321 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4381 aggtgtggga ggttttttaa actagcggcc gcaggaaccc ctagtgatgg agttggccac
     4441 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     4501 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4561 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4621 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4681 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4741 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4801 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     4861 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4921 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     4981 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5041 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     5101 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5161 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5221 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5281 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5341 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5401 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5461 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5521 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5581 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5641 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5701 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5761 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5821 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5881 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5941 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6001 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6061 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6121 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6181 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6241 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6301 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6361 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6421 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7141 gctcacatgt
//
