LOCUS       Exported                9347 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  pGAL4-piggyBac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4885..4924
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4946..4951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..895
                     /label=CMV promoter
                     /label=CMV
                     /note="ApEinfo_fwdcolor=pink ApEinfo_revcolor=pink
                     vntifkey=29"
     primer_bind     863..886
                     /label=CMVF
     primer_bind     863..882
                     /label=T7
                     /note="ApEinfo_fwdcolor=#00ff80 ApEinfo_revcolor=green"
     misc_feature    961..3315
                     /label=SP1
                     /note="ApEinfo_fwdcolor=#00ffff ApEinfo_revcolor=green"
     primer_bind     1273..1293
                     /label=SP1F1
     primer_bind     complement(1316..1336)
                     /label=SP1R1
     primer_bind     1928..1949
                     /label=Sp1F2
     primer_bind     complement(2901..2920)
                     /label=Sp1R2
     primer_bind     3237..3254
                     /label=SP1F3
     misc_feature    3316..3369
                     /label=18aa
     misc_feature    3316..3358
                     /label=18aa linker
                     /note="ApEinfo_fwdcolor=#ffff00 ApEinfo_revcolor=green"
     misc_feature    3348..3358
                     /label=15bp for infusion
     misc_feature    3361..3369
                     /label=18aa linker
                     /note="ApEinfo_fwdcolor=#ffff00 ApEinfo_revcolor=green"
     misc_feature    3370..3393
                     /label=NLS from Ivics et al
     misc_feature    3394..3423
                     /label=10Gs from Ivics codon optimized for mm
     CDS             3424..4440
                     /codon_start=1
                     /label=SB100X CDS
                     /note="SB100X CDS color: #ff9966 ApEinfo_fwdcolor=#0000ff 
                     ApEinfo_revcolor=#ff80ff"
                     /translation="GKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRKY
                     KHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVKR
                     VLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHND
                     HRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDILK
                     QHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENLW
                     AELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY"
     primer_bind     complement(3981..3999)
                     /label=SB100R1
     misc_feature    4445..4459
                     /label=15bp for infusion
     misc_feature    4487..4712
                     /label=BGH polyA
                     /label=BGH\polyA
                     /note="ApEinfo_fwdcolor=pink ApEinfo_revcolor=pink
                     vntifkey=21"
     primer_bind     complement(4715..4735)
                     /label=BGHR3
     misc_feature    complement(4943..5953)
                     /label=yeastTRP1
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#ffff80"
     misc_feature    complement(4946..4951)
                     /label=yeastTRP1
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#ffff80"
     misc_feature    complement(4998..5672)
                     /label=TRP1cds
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#ff8000"
     misc_feature    complement(5956..6726)
                     /label=CEN
                     /note="ApEinfo_fwdcolor=#ff0080 ApEinfo_revcolor=#ff0080"
     misc_feature    complement(6206..6582)
                     /label=ARS209
                     /note="ApEinfo_fwdcolor=#ffa477 ApEinfo_revcolor=#ff9b6a"
     misc_feature    complement(7593..8189)
                     /label=ColE1 ori
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#8080ff"
     misc_feature    complement(8351..9211)
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#c100c1"
     misc_feature    complement(9232..9281)
                     /label=AmpR_Promoter
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=#ff80ff"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcacc
      961 atgagcgacc aagatcactc catggatgaa atgacagctg tggtgaaaat tgaaaaagga
     1021 gttggtggca ataatggggg caatggtaat ggtggtggtg ccttttcaca ggctcgaagt
     1081 agcagcacag gcagtagcag cagcactgga ggaggagggc aggagtccca gccatcccct
     1141 ttggctctgc tggcagcaac ttgcagcaga attgagtcac ccaatgagaa cagcaacaac
     1201 tcccagggcc cgagtcagtc agggggaaca ggtgagcttg acctcacagc cacacaactt
     1261 tcacagggtg ccaatggctg gcagatcatc tcttcctcct ctggggctac ccctacctca
     1321 aaggaacaga gtggcagcag taccaatggc agcaatggca gtgagtcttc caagaatcgc
     1381 acagtctctg gtgggcagta tgttgtggct gccgctccca acttacagaa ccagcaagtt
     1441 ctgacaggac tacctggagt gatgcctaat attcagtatc aagtaatccc acagttccag
     1501 accgttgatg ggcaacagct gcagtttgct gccactgggg cccaagtgca gcaggatggt
     1561 tctggtcaaa tacagatcat accaggtgca aaccaacaga ttatcacaaa tcgaggaagt
     1621 ggaggcaaca tcattgctgc tatgccaaac ctactccagc aggctgtccc cctccaaggc
     1681 ctggctaata atgtactctc aggacagact cagtatgtga ccaatgtacc agtggccctg
     1741 aatgggaaca tcaccttgct acctgtcaac agcgtttctg cagctacctt gactcccagc
     1801 tctcaggcag tcacgatcag cagctctggg tcccaggaga gtggctcaca gcctgtcacc
     1861 tcagggacta ccatcagttc tgccagcttg gtatcatcac aagccagttc cagctccttt
     1921 ttcaccaatg ccaatagcta ctcaactact actaccacca gcaacatggg aattatgaac
     1981 tttactacca gtggatcatc agggaccaac tctcaaggcc agacacccca gagggtcagt
     2041 gggctacagg ggtctgatgc tctgaacatc cagcaaaacc agacatctgg aggctcattg
     2101 caagcaggcc agcaaaaaga aggagagcaa aaccagcaga cacagcagca acaaattctt
     2161 atccagcctc agctagttca agggggacag gccctccagg ccctccaagc agcaccattg
     2221 tcagggcaga cctttacaac tcaagccatc tcccaggaaa ccctccagaa cctccagctt
     2281 caggctgttc caaactctgg tcccatcatc atccggacac caacagtggg gcccaatgga
     2341 caggtcagtt ggcagactct acagctgcag aacctccaag ttcagaaccc acaagcccaa
     2401 acaatcacct tagccccaat gcagggtgtt tccttggggc agaccagcag cagcaacacc
     2461 actctcacac ccattgcctc agctgcttcc attcctgctg gcacagtcac tgtgaatgct
     2521 gctcaactct cctccatgcc aggcctccag accattaacc tcagtgcatt gggtacttca
     2581 ggaatccagg tgcacccaat tcaaggcctg ccgttggcta tagcaaatgc cccaggtgat
     2641 catggagctc agcttggtct ccatggggct ggtggtgatg gaatacatga tgacacagca
     2701 ggtggagagg aaggagaaaa cagcccagat gcccaacccc aagccggtcg gaggacccgg
     2761 cgggaagcat gcacctgccc ctactgtaaa gacagtgaag gaaggggctc gggggatcct
     2821 ggcaaaaaga aacagcatat ttgccacatc caaggctgtg ggaaagtgta tggcaagacc
     2881 tctcacctgc gggcacactt gcgctggcat acaggcgaga ggccatttat gtgtacctgg
     2941 tcatactgtg ggaaacgctt cacacgttcg gatgagctac agaggcacaa acgtacacac
     3001 acaggtgaga agaaatttgc ctgccctgag tgtcctaagc gcttcatgag gagtgaccac
     3061 ctgtcaaaac atatcaagac ccaccagaat aagaagggag gcccaggtgt agctctgagt
     3121 gtgggcactt tgcccctgga cagtggggca ggttcagaag gcagtggcac tgccactcct
     3181 tcagccctta ttaccaccaa tatggtagcc atggaggcca tctgtccaga gggcattgcc
     3241 cgtcttgcca acagtggcat caacgtcatg caggtggcag atctgcagtc cattaatatc
     3301 agtggcaatg gcttcaagtt gggcggcggc gcccccgccg tgggcggcgg acccaaggcc
     3361 gcggataaac caaaaaagaa aagaaaacta gcaggtggcg gaggaggcgg tgggggtggc
     3421 ggtggaaaat caaaagaaat cagccaagac ctcagaaaaa gaattgtaga cctccacaag
     3481 tctggttcat ccttgggagc aatttccaaa cgcctggcgg taccacgttc atctgtacaa
     3541 acaatagtac gcaagtataa acaccatggg accacgcagc cgtcataccg ctcaggaagg
     3601 agacgcgttc tgtctcctag agatgaacgt actttggtgc gaaaagtgca aatcaatccc
     3661 agaacaacag caaaggacct tgtgaagatg ctggaggaaa caggtacaaa agtatctata
     3721 tccacagtaa aacgagtcct atatcgacat aacctgaaag gccactcagc aaggaagaag
     3781 ccactgctcc aaaaccgaca taagaaagcc agactacggt ttgcaactgc acatggggac
     3841 aaagatcgta ctttttggag aaatgtcctc tggtctgatg aaacaaaaat agaactgttt
     3901 ggccataatg accatcgtta tgtttggagg aagaaggggg aggcttgcaa gccgaagaac
     3961 accatcccaa ccgtgaagca cgggggtggc agcatcatgt tgtgggggtg ctttgctgca
     4021 ggagggactg gtgcacttca caaaatagat ggcatcatgg acgccgtgca gtatgtggat
     4081 atattgaagc aacatctcaa gacatcagtc aggaagttaa agcttggtcg caaatgggtc
     4141 ttccaacacg acaatgaccc caagcatact tccaaagttg tggcaaaatg gcttaaggac
     4201 aacaaagtca aggtattgga gtggccatca caaagccctg acctcaatcc tatagaaaat
     4261 ttgtgggcag aactgaaaaa gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta
     4321 caccagctct gtcaggagga atgggccaaa attcacccaa attattgtgg gaagcttgtg
     4381 gaaggctacc cgaaacgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac
     4441 tagctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4501 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4561 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4621 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4681 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4741 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4801 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4861 ccttcctttc tcgccacgtt cgccacccta aagggagccc ccgatttaga gcttgacggg
     4921 gaaatgtgcg gtatttcaca ccgcatagat cggcaagtgc acaaacaata cttaaataaa
     4981 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
     5041 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
     5101 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt
     5161 tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct
     5221 gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca
     5281 ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg
     5341 aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa
     5401 tcattgacca gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat
     5461 ttcggagtgc ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata
     5521 accgggtcaa ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg
     5581 gaatctagag cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga
     5641 ccagaactac ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc
     5701 acgtatactc acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt
     5761 ttttcgaccg aattaattct taatcggcaa aaaaagaaaa gctccggatc aagattgtac
     5821 gtaaggtgac aagctatttt tcaataaaga atatcttcca ctactgccat ctggcgtcat
     5881 aactgcaaag tacacatata ttacgatgct gtctattaaa tgcttcctat attatatata
     5941 tagtaatgtc gtttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6001 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6061 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6121 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6181 gtcatgataa taatggtttc ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt
     6241 atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg
     6301 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     6361 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     6421 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     6481 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     6541 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     6601 tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt
     6661 tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga
     6721 cccaggccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7021 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7081 agcaggcaga agtatgcaaa gcacattcta gttgtggttt gtccaaactc atcaatgtat
     7141 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8101 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     8161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
