LOCUS       Exported                4900 bp ds-DNA     circular SYN 05-JUN-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          8..638
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..620
                     /label=PAC
                     /note="PAC"
     misc_feature    complement(639..648)
                     /label=bar02
                     /note="bar02"
     misc_feature    complement(649..685)
                     /label=IllumiaSeq2
                     /note="IllumiaSeq2"
     misc_feature    complement(686..693)
                     /label=Pme1
                     /note="Pme1"
     misc_feature    701..1108
                     /label=Gene Block
                     /note="Gene Block"
     misc_feature    701..715
                     /label=15bp for infusion
                     /note="15bp for infusion"
     misc_feature    complement(710..717)
                     /label=Mre
                     /note="Mre"
     misc_feature    727..768
                     /label=5'-proxITR
                     /note="5'-proxITR"
     misc_feature    770..801
                     /label=Li
                     /note="Li"
     misc_feature    774..998
                     /label=5'-ITR
                     /note="5'-ITR"
     misc_feature    961..964
                     /label=possible barcode site?
                     /note="possible barcode site?"
     misc_feature    967..998
                     /label=Lo
                     /note="Lo"
     misc_feature    969..995
                     /label=primer
                     /note="primer"
     misc_feature    999..1002
                     /label=tata1
                     /note="tata1"
     misc_feature    1009..1091
                     /label=N117 Sm1 Rz
                     /note="N117 Sm1 Rz"
                     /note="Hammerhead ribozyme N117"
     misc_feature    1094..1108
                     /label=15bp for infusion
                     /note="15bp for infusion"
     misc_feature    1117..1235
                     /label=5S ribosomal RNA
                     /note="5S ribosomal RNA"
     rep_origin      complement(1336..1441)
                     /direction=LEFT
                     /label=Col E1 origin
                     /note="Col E1 origin"
     rep_origin      complement(1442..2003)
                     /direction=LEFT
                     /label=Col E1 origin
                     /note="Col E1 origin"
     CDS             complement(2151..3011)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3012..3116)
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     misc_feature    3347..3719
                     /label=3ITR gene block
                     /note="3ITR gene block"
     misc_feature    3347..3361
                     /label=15bp for infusion
                     /note="15bp for infusion"
     misc_feature    3365..3387
                     /label=primer binding to Z construct
                     /note="primer binding to Z construct"
     misc_feature    3388..3391
                     /label=ttaa2
                     /note="ttaa2"
     misc_feature    3392..3616
                     /label=3'-ITR
                     /note="3'-ITR"
     misc_feature    3392..3423
                     /label=RO
                     /note="RO"
     misc_feature    3617..3658
                     /label=3'-proxITR
                     /note="3'-proxITR"
     misc_feature    3667..3703
                     /label=primer binding to Z construct
                     /note="primer binding to Z construct"
     misc_feature    3705..3719
                     /label=15bp for infusion
                     /note="15bp for infusion"
     promoter        join(3710..4900,1..7)
                     /label=EF1a Promoter
                     /label=EF1a\Promoter
                     /note="EF1a Promoter"
     misc_feature    3710..3719
                     /label=removable
                     /note="removable"
ORIGIN
        1 gtcgtgaaaa ctacgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
       61 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
      121 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
      181 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
      241 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
      301 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
      361 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
      421 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
      481 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
      541 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
      601 cccgcaagcc cggtgcctga taacaactcg agtaccggct taagtgatag atcggaagag
      661 cggttcagca ggaatgccga gaccggttta aactcagtgt ccatagcgac gccggcgcga
      721 taagctggac cccttaccga aacatcgccg cattctgcag aggagtcgaa cttagtgtat
      781 gtaaacttct gacccactgg aattgtgata cagtgaatta taagtgaaat aatctgtctg
      841 taaacaattg ttggaaaaat gacttgtgtc atgcacaaag tagatgtcct aactgacttg
      901 ccaaaactat tgtttgttaa caagaaattt gtggagtagt tgaaaaacga gttttaatga
      961 ctccaactta agtgtatgta aacttccgac ttcaactgta tagagctcct gagatgcagg
     1021 tacatcccac tgatgagtcc caaataggac gaaacgcgct tcggtgcgtc tgggattcca
     1081 ctgctatcca cgcggccgca cggcattacg ttagacgtct aggccacacc accctgaagg
     1141 cgcctgctcg cctctgatct gttgaagcta agcagggtcg gtcctggtta gtacttggat
     1201 gggactccgc ctggtaatag ccggtaccgt aggcttgcat ggtcatagct gtttccttgc
     1261 gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa
     1321 gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1381 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1561 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1801 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2101 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2221 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2281 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3121 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3181 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3241 aatcaaaaga atagaccgag atagggttga gtggccgcta cagggcgctc ccattcgcca
     3301 ttcaggctgc gcaactgttg ggaagggcgt ttcggtgcgg gcctcttcgc tattacgcca
     3361 gctgggcctt ttgctggcct tttgctctat acagttgaag tcggaagttt acatacacct
     3421 tagccaaata catttaaact cactttttca caattcctga catttaatcc tagtaaaaat
     3481 tccctgtctt aggtcagtta ggatcaccac tttattttaa gaatgtgaaa tatcagaata
     3541 atagtagaga gaatgattca tttcagcttt tatttctttc atcacattcc cagtgggtca
     3601 gaagtttaca tacactcgac tcctctgcag aatgcggcga tgtttcggta aggggtcctt
     3661 aattaatacc acatttgtag aggttttact tgctttaaaa aactctagag aagagcatgc
     3721 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3781 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     3841 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3901 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     3961 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4021 aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     4081 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     4141 aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     4201 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     4261 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     4321 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     4381 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     4441 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     4501 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     4561 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     4621 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     4681 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     4741 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     4801 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     4861 ctcagacagt ggttcaaagt ttttttcttc catttcaggt
//
