LOCUS       Exported                6466 bp ds-DNA     circular SYN 05-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Matthias Arnold
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="synthetic DNA construct"
     source          join(2215..6466,1..2214)
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          join(2215..6466,1..2214)
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     source          4300..4337
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..44
                     /label=tR2 lambda phage terminator
     primer_bind     392..409
                     /label=M13-21
     misc_feature    448..456
                     /label=vector ID
     misc_feature    457..588
                     /label=5'ITR
                     /note="5'ITR"
     misc_feature    615..716
                     /label=CAG Enhancer
                     /note="CAG Enhancer"
     primer_bind     701..736
                     /label=AAVSRTF4
     misc_feature    701..716
                     /label=Righthomoarm
     misc_feature    complement(735..936)
                     /label=pBac 3'-LTR
     promoter        1024..2221
                     /label=EF1a Promoter
                     /label=EF1a\Promoter
     misc_feature    1024..1033
                     /label=removable
     misc_feature    1237..1269
                     /label=exon1
     misc_feature    1270..2212
                     /label=intron1
                     /note="EF-1a Intron A"
     misc_feature    2235..3665
                     /label=tdTomato
     misc_feature    2235..2237
                     /label=PAC
                     /note="PuroR"
     misc_feature    complement(3681..3690)
                     /label=bar02
     misc_feature    complement(3691..3727)
                     /label=IllumiaSeq2
     misc_feature    complement(3728..3735)
                     /label=Pme1
     misc_feature    complement(3752..3759)
                     /label=Mre
     misc_feature    3769..4074
                     /label=PB 5' TR
     misc_feature    4023..4037
                     /label=15bp for infusion
     misc_feature    4026..4041
                     /label=RightHomoArm
     misc_feature    4059..4074
                     /label=Required TR
     misc_feature    4075..4078
                     /label=TTAA
     misc_feature    4085..4167
                     /label=N117 Sm1 Rz
                     /note="Hammerhead ribozyme N117"
     misc_feature    4168..4183
                     /label=LeftHomoArm
     misc_feature    4170..4184
                     /label=15bp for infusion
     misc_feature    4193..4299
                     /label=5S ribosomal RNA
     misc_feature    4300..4311
                     /label=5S ribosomal RNA
     primer_bind     complement(4319..4352)
                     /label=AAVSRTR4
     misc_feature    4338..4359
                     /label=bgh polya signal
                     /note="bgh polya signal"
     misc_feature    4338..4353
                     /label=Lefthomoarm
     misc_recomb     4382..4512
                     /label=3' ITR
                     /note="3' ITR"
     misc_feature    4522..4530
                     /label=vector ID
     primer_bind     complement(4584..4602)
                     /label=AAVSRTR4
     terminator      complement(4677..4720)
                     /label=rrnB T1 transcription terminator
     rep_origin      complement(4721..5388)
                     /direction=LEFT
                     /label=Col E1 origin
     CDS             complement(5536..6396)
                     /codon_start=1
                     /label=ampR - bla
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6397..6466)
                     /label=bla promoter
ORIGIN
        1 gctaataaca ggcctgctgg taatcgcagg cctttttatt tggtgtattt agaaaaataa
       61 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
      121 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      181 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtggccgc
      241 tacagggcgc tcccattcgc cattcaggct gcgcaactgt tgggaagggc gtttcggtgc
      301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgacg
      421 taatacgact cactataggg cgaattgtag gcggaaggct gcgcgctcgc tcgctcactg
      481 aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg
      541 agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctat tgacgtcaga
      601 tcgctaaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatacgtc
      721 aaggccgcat ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
      781 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
      841 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
      901 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
      961 caaaactttt atcgaattcc tgcagcccag cttgatctta ggtccctcga cgaattctct
     1021 agagaagagc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     1081 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     1141 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     1201 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     1261 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     1321 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     1381 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     1441 tcgtgcttga gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca
     1501 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     1561 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1621 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1681 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1741 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1801 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1861 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1921 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1981 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     2041 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     2101 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     2161 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     2221 aaaactacgc caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
     2281 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     2341 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     2401 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     2461 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     2521 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     2581 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     2641 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     2701 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     2761 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     2821 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2881 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
     2941 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     3001 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     3061 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     3121 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     3181 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     3241 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     3301 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     3361 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     3421 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     3481 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     3541 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     3601 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     3661 agtaacaact cgagtaccgg cttaagtgat agatcggaag agcggttcag caggaatgcc
     3721 gagaccggtt taaactcagt gtccatagcg acgccggcgc gataagctat ctataacaag
     3781 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     3841 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     3901 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     3961 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     4021 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaga
     4081 gctcctgaga tgcaggtaca tcccactgat gagtcccaaa taggacgaaa cgcgcttcgg
     4141 tgcgtctggg attccactgc tatccacgcg gccgcacggc attacgttag acgtctaggc
     4201 cacaccaccc tgaaggcgcc tgctcgcctc tgatctgttg aagctaagca gggtcggtcc
     4261 tggttagtac ttggatggga ctccgcctgg taatagccgg taccgtaggc ttgcatggtc
     4321 atagctgttt ccttgcgctg gggatgcggt gggctctata gagagtcgat cgtgtgatcg
     4381 aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4441 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4501 cgagcgcgca gcatgtagag attccgccta tcactgcccg ctttccagtc gggaaacctg
     4561 tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc gctctccgct
     4621 tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt gcctaaataa
     4681 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat tgagcaaaag gccagcaaaa
     4741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5641 cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga
     5701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaa
//
