LOCUS       Exported                7632 bp ds-DNA     circular SYN 12-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       OpAAV-ChrimsonS169A_2.0 6268 bp ds-DNA     linear       
            30-JAN-2019

            DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=rep origin
     CDS             complement(941..1801)
                     /label=bla CDS
     promoter        complement(1843..1871)
                     /label=amp prom
     repeat_region   2458..2647
                     /label=ITR
     promoter        2688..3135
                     /label=Human Synapsin I promoter
                     /note="Human Synapsin I promoter"
     gene            3148..4020
                     /label=GtACR2
                     /note="GtACR2"
     gene            4036..4095
                     /label=TS
                     /note="TS"
     gene            4105..4815
                     /label=mCerulean3
                     /note="mCerulean3"
     gene            4822..5133
                     /label=beta subunit HK-ATPase
                     /note="beta subunit HK-ATPase"
     gene            5174..6219
                     /label=Chrimson
                     /note="Chrimson"
     gene            5770..5772
                     /label=#b7e6d7
     misc_feature    6226..6420
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
     enhancer        6441..7030
                     /label=WPRE
     polyA_signal    7071..7284
                     /label=bGH-PolyA
     repeat_region   complement(7389..7578)
                     /label=ITR
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa tctagactgc agagggccct gcgtatgagt gcaagtgggt
     2701 tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg
     2761 gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga
     2821 aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg
     2881 cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc
     2941 gccggtcccc cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc
     3001 ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc
     3061 tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg
     3121 tgcctgagag cgcagtcgag aggatccatg gcatcacagg tcgtctatgg ggaatgggca
     3181 tcaactcaca ccgagtgcta caatatgtcc agaatcgact ccaccttcgt ctcactgctg
     3241 cagctggtgt gggccgtggt ctcaggctgc cagacaatct tcatgattag cagggctccc
     3301 aaggtccctt gggagtccgt gtacctgccc tttgtcgaat ctatcactta tgcactggcc
     3361 agtactggca acggaaccct gcagatgagg gacggcagat tctttccttg gagccgaatg
     3421 gcctcctggc tgtgcacctg tccaatcatg ctgggacaga tttctaacat ggctctggtg
     3481 aagtacaaaa gtatcccact gaatcccatt gcacaggccg cttccatcat tagagtggtc
     3541 atgggaatca ccgctacaat ttctcccgca gagtatatga agtggctgtt ctttttcttt
     3601 ggggccacct gcctggtgtt cgagtacagt gtggtcttca caatctttca agtggggctg
     3661 tatggctttg aatctgtcgg gacacctctg gcacagaagg tggtggtgag gatcaagatg
     3721 ctgcgcctga tcttctttat tgcttggact atgttcccaa tcgtgtggct gatttcaccc
     3781 accggagtct gcgtgatcca cgagaacgtg agcgccattc tgtacctgct ggctgacggc
     3841 ctgtgcaaaa atacatatgg agtgatcctg tggagcactg cctggggagt gctggaaggg
     3901 aagtgggacc ccgcatgtct gccagggcag gagaaacccg aagccgacga tccttttggc
     3961 ctgaatcacg agaagaatgc ccctcctaac gacgaagtca atatcagaat gtttggaagg
     4021 ggacgtacgg cggccaagag caggatcacc agcgagggcg agtacatccc cctggaccag
     4081 atcgacatca acgtggcggt acccgtgagc aagggcgagg agctgttcac cggggtggtg
     4141 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4201 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4261 ctgcccgtgc cctggcccac cctcgtgacc accctgagct ggggcgtgca gtgcttcgcc
     4321 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4381 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4441 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4501 gacggcaaca tcctggggca caagctggag tacaacgcca tccacggcaa cgtctatatc
     4561 accgccgaca agcagaagaa cggcatcaag gccaacttcg gcctcaactg caacatcgag
     4621 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4681 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     4741 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4801 atggacgagc tgtataccgg tgcagccctg caggagaaga agtcatgcag ccagcgcatg
     4861 gccgaattcc ggcaatactg ttggaacccg gacactgggc agatgctggg ccgcacccca
     4921 gcccggtggg tgtggatcag cctgtactat gcagctttct acgtggtcat gactgggctc
     4981 tttgccttgt gcatctatgt gctgatgcag accattgatc cctacacccc cgactaccag
     5041 gaccagttaa agtcaccggg ggtaaccttg agaccggatg tgtatgggga aagagggctg
     5101 cagatttcct acaacatctc tgaaaacagc tctagacagg cccagatcac cggacgtccg
     5161 gagggagctg tacaagagct gatcagcagc gccaccagat ctctgtttgc cgccggaggc
     5221 atcaaccctt ggcctaaccc ctaccaccac gaggacatgg gctgtggagg aatgacacct
     5281 acaggcgagt gcttcagcac cgagtggtgg tgtgaccctt cttacggact gagcgacgcc
     5341 ggatacggat attgcttcgt ggaggccaca ggcggctacc tggtcgtggg agtggagaag
     5401 aagcaggctt ggctgcacag cagaggcaca ccaggagaaa agatcggcgc ccaggtctgc
     5461 cagtggattg ctttcagcat cgccatcgcc ctgctgacat tctacggctt cagcgcctgg
     5521 aaggccactt gcggttggga ggaggtctac gtctgttgcg tcgaggtgct gttcgtgacc
     5581 ctggagatct tcaaggagtt cagcagcccc gccacagtgt acctgtctac cggcaaccac
     5641 gcctattgcc tgcgctactt cgagtggctg ctgtcttgcc ccgtgatcct gatcaagctg
     5701 agcaacctga gcggcctgaa gaacgactac agcaagcgga ccatgggcct gatcgtgtct
     5761 tgcgtgggaa tgatcgtgtt cggcatggcc gcaggactgg ctaccgattg gctcaagtgg
     5821 ctgctgtata tcgtgtcttg catctacggc ggctacatgt acttccaggc cgccaagtgc
     5881 tacgtggaag ccaaccacag cgtgcctaaa ggccattgcc gcatggtcgt gaagctgatg
     5941 gcctacgctt acttcgcctc ttggggcagc tacccaatcc tctgggcagt gggaccagaa
     6001 ggactgctga agctgagccc ttacgccaac agcatcggcc acagcatctg cgacatcatc
     6061 gccaaggagt tttggacctt cctggcccac cacctgagga tcaagatcca cgagcacatc
     6121 ctgatccacg gcgacatccg gaagaccacc aagatggaga tcggaggcga ggaggtggaa
     6181 gtggaagagt tcgtggagga ggaggacgag gacacagtgc tcgagcagag ccagcccatc
     6241 ctgaacacca aggagatggc cccccagagc aagccccccg aggagctgga gatgagcagc
     6301 atgcccagcc ccgtggcccc cctgcccgcc cgcaccgagg gcgtgatcga catgcgcagc
     6361 atgagcagca tcgacagctt catcagctgc gccaccgact tccccgaggc cacccgcttc
     6421 tgatctagaa agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     6481 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6541 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6601 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6661 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6721 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6781 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6841 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6901 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6961 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7021 gcctccccgc atcgataccg tcgacccggg cggccgctca taaagctcga ctgtgccttc
     7081 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7141 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7201 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7261 tagcaggcat gctggggatg cggtccggac tgtacaattg cattcatttt atgtttcagg
     7321 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     7381 tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt
     7441 aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     7501 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     7561 agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7621 gtattgggcg ct
//
