LOCUS Exported 7822 bp ds-DNA circular SYN 12-JUN-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7822) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 12, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com COMMENT LOCUS Exported 5459 bp ds-DNA circular SYN 16-JUN-2017 DEFINITION GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element. ACCESSION . VERSION . KEYWORDS pAAV-mDlx-GFP-Fishell-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5459) AUTHORS Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G, Fishell G TITLE A viral strategy for targeting and manipulating interneurons across vertebrate species. JOURNAL Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430. PUBMED 27798629 REFERENCE 2 (bases 1 to 5459) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 16, 2017 from SnapGene Console 4.0.0a8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7822 /organism="synthetic DNA construct" /label=synthetic DNA construct repeat_region 1..141 /label=inverted terminal repeat of /note="inverted terminal repeat of adeno-associated virus serotype 2" primer_bind complement(23..34) /label=L4440 vector, forward primer /note="L4440 vector, forward primer" primer_bind 84..95 /label=L4440 vector, forward primer /note="L4440 vector, forward primer" enhancer 170..699 /label=mDlx /note="mDlx" intron 860..992 /label=chimera between introns from human /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 975..994 /label=Synthetic intron, forward primer /note="Synthetic intron, forward primer" misc_feature 1040..1912 /label=GtACR2 /note="GtACR2" misc_feature 1928..1987 /label=TS /note="TS" misc_feature 1997..2707 /label=mCerulean3 /note="mCerulean3" misc_feature 2714..3025 /label=beta subunit HK-ATPase /note="beta subunit HK-ATPase" misc_feature 3066..4111 /label=Chrimson /note="Chrimson" misc_feature 4168..4756 /label=woodchuck hepatitis virus /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4221..4241) /label=WPRE, reverse primer /note="WPRE, reverse primer" CDS complement(4639..4650) /codon_start=1 /label=Factor Xa recognition and cleavage site /translation="IEGR" polyA_signal 4925..5006 /label=SV40 polyadenylation signal /note="SV40 polyadenylation signal" primer_bind complement(4962..4981) /label=SV40 polyA, reverse primer /note="SV40 polyA, reverse primer" primer_bind 5016..5035 /label=SV40 polyA terminator, reverse primer /note="SV40 polyA terminator, reverse primer" repeat_region 5085..5225 /label=inverted terminal repeat of /note="inverted terminal repeat of adeno-associated virus serotype 2" primer_bind complement(5131..5142) /label=L4440 vector, forward primer /note="L4440 vector, forward primer" primer_bind 5192..5203 /label=L4440 vector, forward primer /note="L4440 vector, forward primer" rep_origin 5300..5755 /direction=RIGHT /label=f1 bacteriophage origin of replication /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5387..5406) /label=F1 origin, reverse primer /note="F1 origin, reverse primer" primer_bind 5597..5618 /label=F1 origin, forward primer /note="F1 origin, forward primer" primer_bind complement(5772..5791) /label=pRS vectors /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5891..5913 /label=pGEX vectors, reverse primer /note="pGEX vectors, reverse primer" primer_bind complement(5951..5969) /label=pBR322 vectors /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6037..6141 /label=bla CDS 6142..7002 /codon_start=1 /label=bla /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6360..6379) /label=Ampicillin resistance gene /note="Ampicillin resistance gene, reverse primer" rep_origin 7173..7761 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7662..7681 /label=pBR322 origin, forward primer /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagaattct atacactcac 181 agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg gtttcgatat 241 atggtagctt tttctctaac ataatttgaa taattcagca aagccctact accagctgta 301 cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt caattttagg 361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct 421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac 481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct 541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt 601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga 661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag 721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct 781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg 841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata 901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct 961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc 1021 ttaagaaact agtggatcca tggcatcaca ggtcgtctat ggggaatggg catcaactca 1081 caccgagtgc tacaatatgt ccagaatcga ctccaccttc gtctcactgc tgcagctggt 1141 gtgggccgtg gtctcaggct gccagacaat cttcatgatt agcagggctc ccaaggtccc 1201 ttgggagtcc gtgtacctgc cctttgtcga atctatcact tatgcactgg ccagtactgg 1261 caacggaacc ctgcagatga gggacggcag attctttcct tggagccgaa tggcctcctg 1321 gctgtgcacc tgtccaatca tgctgggaca gatttctaac atggctctgg tgaagtacaa 1381 aagtatccca ctgaatccca ttgcacaggc cgcttccatc attagagtgg tcatgggaat 1441 caccgctaca atttctcccg cagagtatat gaagtggctg ttctttttct ttggggccac 1501 ctgcctggtg ttcgagtaca gtgtggtctt cacaatcttt caagtggggc tgtatggctt 1561 tgaatctgtc gggacacctc tggcacagaa ggtggtggtg aggatcaaga tgctgcgcct 1621 gatcttcttt attgcttgga ctatgttccc aatcgtgtgg ctgatttcac ccaccggagt 1681 ctgcgtgatc cacgagaacg tgagcgccat tctgtacctg ctggctgacg gcctgtgcaa 1741 aaatacatat ggagtgatcc tgtggagcac tgcctgggga gtgctggaag ggaagtggga 1801 ccccgcatgt ctgccagggc aggagaaacc cgaagccgac gatccttttg gcctgaatca 1861 cgagaagaat gcccctccta acgacgaagt caatatcaga atgtttggaa ggggacgtac 1921 ggcggccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat 1981 caacgtggcg gtacccgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2041 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 2101 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2161 gccctggccc accctcgtga ccaccctgag ctggggcgtg cagtgcttcg cccgctaccc 2221 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 2281 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2341 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2401 catcctgggg cacaagctgg agtacaacgc catccacggc aacgtctata tcaccgccga 2461 caagcagaag aacggcatca aggccaactt cggcctcaac tgcaacatcg aggacggcag 2521 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 2581 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg 2641 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 2701 gctgtatacc ggtgcagccc tgcaggagaa gaagtcatgc agccagcgca tggccgaatt 2761 ccggcaatac tgttggaacc cggacactgg gcagatgctg ggccgcaccc cagcccggtg 2821 ggtgtggatc agcctgtact atgcagcttt ctacgtggtc atgactgggc tctttgcctt 2881 gtgcatctat gtgctgatgc agaccattga tccctacacc cccgactacc aggaccagtt 2941 aaagtcaccg ggggtaacct tgagaccgga tgtgtatggg gaaagagggc tgcagatttc 3001 ctacaacatc tctgaaaaca gctctagaca ggcccagatc accggacgtc cggagggagc 3061 tgtacaagag ctgatcagca gcgccaccag atctctgttt gccgccggag gcatcaaccc 3121 ttggcctaac ccctaccacc acgaggacat gggctgtgga ggaatgacac ctacaggcga 3181 gtgcttcagc accgagtggt ggtgtgaccc ttcttacgga ctgagcgacg ccggatacgg 3241 atattgcttc gtggaggcca caggcggcta cctggtcgtg ggagtggaga agaagcaggc 3301 ttggctgcac agcagaggca caccaggaga aaagatcggc gcccaggtct gccagtggat 3361 tgctttcagc atcgccatcg ccctgctgac attctacggc ttcagcgcct ggaaggccac 3421 ttgcggttgg gaggaggtct acgtctgttg cgtcgaggtg ctgttcgtga ccctggagat 3481 cttcaaggag ttcagcagcc ccgccacagt gtacctgtct accggcaacc acgcctattg 3541 cctgcgctac ttcgagtggc tgctgtcttg ccccgtgatc ctgatcaagc tgagcaacct 3601 gagcggcctg aagaacgact acagcaagcg gaccatgggc ctgatcgtgt cttgcgtggg 3661 aatgatcgtg ttcggcatgg ccgcaggact ggctaccgat tggctcaagt ggctgctgta 3721 tatcgtgtct tgcatctacg gcggctacat gtacttccag gccgccaagt gctacgtgga 3781 agccaaccac agcgtgccta aaggccattg ccgcatggtc gtgaagctga tggcctacgc 3841 ttacttcgcc tcttggggca gctacccaat cctctgggca gtgggaccag aaggactgct 3901 gaagctgagc ccttacgcca acagcatcgg ccacagcatc tgcgacatca tcgccaagga 3961 gttttggacc ttcctggccc accacctgag gatcaagatc cacgagcaca tcctgatcca 4021 cggcgacatc cggaagacca ccaagatgga gatcggaggc gaggaggtgg aagtggaaga 4081 gttcgtggag gaggaggacg aggacacagt gtgaaagctt aagtttcgcg ccacccctgc 4141 agggaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 4201 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 4261 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 4321 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 4381 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 4441 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 4501 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 4561 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg 4621 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 4681 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 4741 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctgtgat agcggccatc 4801 aagctggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc 4861 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 4921 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5041 atcagcttat cgataccgca tgcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat 5101 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 5161 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct 5221 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 5281 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 5341 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 5401 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 5461 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 5521 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5581 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5641 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 5701 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg 5761 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 5821 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 5881 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 5941 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 6001 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 6061 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 6121 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 6181 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 6241 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 6301 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 6361 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 6421 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 6481 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 6541 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 6601 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 6661 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 6721 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 6781 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 6841 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 6901 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 6961 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 7021 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 7081 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 7141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 7201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 7261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 7321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 7381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 7441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 7501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 7561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 7621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 7681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 7741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 7801 tgctggcctt ttgctcacat gt //