LOCUS       Exported                8031 bp ds-DNA     circular SYN 12-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5459 bp ds-DNA     circular SYN 
            16-JUN-2017

            DEFINITION  GFP expression in forebrain GABA-ergic interneurons 
            under the 

                        control of the mDlx enhancer element.

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-mDlx-GFP-Fishell-1

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5459)

              AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi 
            GA, Guo L, Xu 

                        Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, 
            Jacob AL, Smith 

                        GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, 
            Kotak VC, 

                        Mowery TM, Anderson SA, Callaway EM, Dasen JS, 
            Fitzpatrick D, 

                        Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, 
            Rudy B, Feng G,

                        Fishell G

              TITLE     A viral strategy for targeting and manipulating 
            interneurons across 

                        vertebrate species.

              JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.

              PUBMED    27798629

            REFERENCE   2  (bases 1 to 5459)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Friday, Jun 16, 2017 from SnapGene Console 
            4.0.0a8

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="synthetic DNA construct"
                     /label=synthetic DNA construct
     repeat_region   1..141
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(23..34)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     84..95
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     enhancer        170..699
                     /label=mDlx
                     /note="mDlx"
     intron          860..992
                     /label=chimera between introns from human
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     975..994
                     /label=Synthetic intron, forward primer
                     /note="Synthetic intron, forward primer"
     gene            1049..1921
                     /label=GtACR2
                     /note="GtACR2"
     gene            1937..1996
                     /label=TS
                     /note="TS"
     gene            2006..2716
                     /label=mCerulean3
                     /note="mCerulean3"
     gene            2723..3034
                     /label=beta subunit HK-ATPase
                     /note="beta subunit HK-ATPase"
     gene            3075..4120
                     /label=Chrimson
                     /note="Chrimson"
     gene            3671..3673
                     /label=M201
     misc_feature    4127..4321
                     /label=stKv2.1 GCopt
                     /note="stKv2.1 GCopt"
     misc_feature    4377..4965
                     /label=woodchuck hepatitis virus
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4430..4450)
                     /label=WPRE, reverse primer
                     /note="WPRE, reverse primer"
     CDS             complement(4848..4859)
                     /codon_start=1
                     /label=Factor Xa recognition and cleavage site
                     /translation="IEGR"
     polyA_signal    5134..5215
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5171..5190)
                     /label=SV40 polyA, reverse primer
                     /note="SV40 polyA, reverse primer"
     primer_bind     5225..5244
                     /label=SV40 polyA terminator, reverse primer
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5294..5434
                     /label=inverted terminal repeat of
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     primer_bind     complement(5340..5351)
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     primer_bind     5401..5412
                     /label=L4440 vector, forward primer
                     /note="L4440 vector, forward primer"
     rep_origin      5509..5964
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5596..5615)
                     /label=F1 origin, reverse primer
                     /note="F1 origin, reverse primer"
     primer_bind     5806..5827
                     /label=F1 origin, forward primer
                     /note="F1 origin, forward primer"
     primer_bind     complement(5981..6000)
                     /label=pRS vectors
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6100..6122
                     /label=pGEX vectors, reverse primer
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6160..6178)
                     /label=pBR322 vectors
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6246..6350
                     /label=bla
     CDS             6351..7211
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6569..6588)
                     /label=Ampicillin resistance gene
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7382..7970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7871..7890
                     /label=pBR322 origin, forward primer
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagaattct atacactcac
      181 agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg gtttcgatat
      241 atggtagctt tttctctaac ataatttgaa taattcagca aagccctact accagctgta
      301 cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt caattttagg
      361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct
      421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac
      481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct
      541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt
      601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga
      661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag
      721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct
      781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg
      841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1021 ttaagaaact agtagccacc atggatccat ggcatcacag gtcgtctatg gggaatgggc
     1081 atcaactcac accgagtgct acaatatgtc cagaatcgac tccaccttcg tctcactgct
     1141 gcagctggtg tgggccgtgg tctcaggctg ccagacaatc ttcatgatta gcagggctcc
     1201 caaggtccct tgggagtccg tgtacctgcc ctttgtcgaa tctatcactt atgcactggc
     1261 cagtactggc aacggaaccc tgcagatgag ggacggcaga ttctttcctt ggagccgaat
     1321 ggcctcctgg ctgtgcacct gtccaatcat gctgggacag atttctaaca tggctctggt
     1381 gaagtacaaa agtatcccac tgaatcccat tgcacaggcc gcttccatca ttagagtggt
     1441 catgggaatc accgctacaa tttctcccgc agagtatatg aagtggctgt tctttttctt
     1501 tggggccacc tgcctggtgt tcgagtacag tgtggtcttc acaatctttc aagtggggct
     1561 gtatggcttt gaatctgtcg ggacacctct ggcacagaag gtggtggtga ggatcaagat
     1621 gctgcgcctg atcttcttta ttgcttggac tatgttccca atcgtgtggc tgatttcacc
     1681 caccggagtc tgcgtgatcc acgagaacgt gagcgccatt ctgtacctgc tggctgacgg
     1741 cctgtgcaaa aatacatatg gagtgatcct gtggagcact gcctggggag tgctggaagg
     1801 gaagtgggac cccgcatgtc tgccagggca ggagaaaccc gaagccgacg atccttttgg
     1861 cctgaatcac gagaagaatg cccctcctaa cgacgaagtc aatatcagaa tgtttggaag
     1921 gggacgtacg gcggccaaga gcaggatcac cagcgagggc gagtacatcc ccctggacca
     1981 gatcgacatc aacgtggcgg tacccgtgag caagggcgag gagctgttca ccggggtggt
     2041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2161 gctgcccgtg ccctggccca ccctcgtgac caccctgagc tggggcgtgc agtgcttcgc
     2221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2401 ggacggcaac atcctggggc acaagctgga gtacaacgcc atccacggca acgtctatat
     2461 caccgccgac aagcagaaga acggcatcaa ggccaacttc ggcctcaact gcaacatcga
     2521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2581 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     2641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2701 catggacgag ctgtataccg gtgcagccct gcaggagaag aagtcatgca gccagcgcat
     2761 ggccgaattc cggcaatact gttggaaccc ggacactggg cagatgctgg gccgcacccc
     2821 agcccggtgg gtgtggatca gcctgtacta tgcagctttc tacgtggtca tgactgggct
     2881 ctttgccttg tgcatctatg tgctgatgca gaccattgat ccctacaccc ccgactacca
     2941 ggaccagtta aagtcaccgg gggtaacctt gagaccggat gtgtatgggg aaagagggct
     3001 gcagatttcc tacaacatct ctgaaaacag ctctagacag gcccagatca ccggacgtcc
     3061 ggagggagct gtacaagagc tgatcagcag cgccaccaga tctctgtttg ccgccggagg
     3121 catcaaccct tggcctaacc cctaccacca cgaggacatg ggctgtggag gaatgacacc
     3181 tacaggcgag tgcttcagca ccgagtggtg gtgtgaccct tcttacggac tgagcgacgc
     3241 cggatacgga tattgcttcg tggaggccac aggcggctac ctggtcgtgg gagtggagaa
     3301 gaagcaggct tggctgcaca gcagaggcac accaggagaa aagatcggcg cccaggtctg
     3361 ccagtggatt gctttcagca tcgccatcgc cctgctgaca ttctacggct tcagcgcctg
     3421 gaaggccact tgcggttggg aggaggtcta cgtctgttgc gtcgaggtgc tgttcgtgac
     3481 cctggagatc ttcaaggagt tcagcagccc cgccacagtg tacctgtcta ccggcaacca
     3541 cgcctattgc ctgcgctact tcgagtggct gctgtcttgc cccgtgatcc tgatcaagct
     3601 gagcaacctg agcggcctga agaacgacta cagcaagcgg accatgggcc tgatcgtgtc
     3661 ttgcgtggga atgatcgtgt tcggcatggc cgcaggactg gctaccgatt ggctcaagtg
     3721 gctgctgtat atcgtgtctt gcatctacgg cggctacatg tacttccagg ccgccaagtg
     3781 ctacgtggaa gccaaccaca gcgtgcctaa aggccattgc cgcatggtcg tgaagctgat
     3841 ggcctacgct tacttcgcct cttggggcag ctacccaatc ctctgggcag tgggaccaga
     3901 aggactgctg aagctgagcc cttacgccaa cagcatcggc cacagcatct gcgacatcat
     3961 cgccaaggag ttttggacct tcctggccca ccacctgagg atcaagatcc acgagcacat
     4021 cctgatccac ggcgacatcc ggaagaccac caagatggag atcggaggcg aggaggtgga
     4081 agtggaagag ttcgtggagg aggaggacga ggacacagtg ctcgagcaga gccagcccat
     4141 cctgaacacc aaggagatgg ccccccagag caagcccccc gaggagctgg agatgagcag
     4201 catgcccagc cccgtggccc ccctgcccgc ccgcaccgag ggcgtgatcg acatgcgcag
     4261 catgagcagc atcgacagct tcatcagctg cgccaccgac ttccccgagg ccacccgctt
     4321 ctgatctaga aagctcgcgc cacccctgca gggaattcga tatcaagctt atcgataatc
     4381 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4441 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4501 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4561 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4621 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4681 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4741 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4801 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4861 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4921 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc
     4981 tgctcgagag atctgtgata gcggccatca agctggccgc gactctagat cataatcagc
     5041 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5101 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5221 agttgtggtt tgtccaaact catcaatgta tcagcttatc gataccgcat gcacgtgcgg
     5281 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     5341 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     5401 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     5461 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     5521 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5581 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5641 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5701 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     5761 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5821 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     5881 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5941 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     6001 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6061 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6121 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6181 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6241 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6301 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6361 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6421 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6481 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6541 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6601 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6661 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6721 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6781 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6841 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6901 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6961 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7021 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7081 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7141 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7201 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
