LOCUS       Exported                8679 bp ds-DNA     circular SYN 12-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 12, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       #097\pAAV-Ef-1a-DIO\iChloC\2A\RFP        7293 bp    DNA 
               circular     11-JAN-2016

            SOURCE      

              ORGANISM  

            COMMENT     This file is created by Vector NTI

                        http://www.invitrogen.com/

            COMMENT     ORIGDB|GenBank

            COMMENT     VNTDATE|-11058445|

            COMMENT     VNTDBDATE|-11058445|

            COMMENT     LSOWNER|

            COMMENT     VNTNAME|#097 pAAV-Ef-1a-DIO iChloC 2A RFP|

            COMMENT     VNTAUTHORNAME|Iris Ohmert|

            COMMENT     VNTAUTHORTEL|004940741055085|

            COMMENT     VNTAUTHORFAX|004940741058364|

            COMMENT     VNTAUTHOREML|iohmert@zmnh.uni-hamburg.de|

            COMMENT     VNTAUTHORAD1|Zentrum fuer Molekulare Neurobiologie 
            Hamburg|

            COMMENT     VNTAUTHORAD2|Institut fuer Synaptische Physiologie|

            COMMENT     VNTAUTHORAD3|Falkenried 94/Martinistr.85|

            COMMENT     VNTAUTHORAD4|D-20251 Hamburg|

            COMMENT     VNTOAUTHORNAME|ISP|

            COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)

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            COMMENT                          ""Created: 01/11/16 09:40"" 1) 

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            COMMENT                            1 5 ""TCTAGA"" ""AGATCT"") 

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FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        249..1419
                     /label=EF1a Promoter
     misc_feature    1450..1483
                     /label=LoxP 2722
     misc_feature    1534..1567
                     /label=loxP
     misc_feature    complement(1582..2627)
                     /label=Chrimson
                     /note="Chrimson"
     misc_feature    complement(2668..2979)
                     /label=beta subunit HK-ATPase
                     /note="beta subunit HK-ATPase"
     misc_feature    complement(2986..3696)
                     /label=mCerulean3
                     /note="mCerulean3"
     misc_feature    complement(3706..3765)
                     /label=TS
                     /note="TS"
     misc_feature    complement(3781..4653)
                     /label=gtacr2
                     /note="gtacr2"
     misc_feature    4664..4697
                     /label=loxP 2722
     misc_feature    complement(4748..4781)
                     /label=loxP
     misc_feature    4802..5397
                     /label=WRE
     misc_feature    5424..5901
                     /label=hGH PolyA
     misc_feature    5942..6081
                     /label=right ITR
     rep_origin      6174..6479
                     /direction=RIGHT
                     /label=f1 Origin
     misc_feature    6999..7852
                     /label=AmpR
     rep_origin      8007..8673
                     /direction=RIGHT
                     /label=pUC Origin
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagagtc
     1441 gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga
     1501 ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg
     1561 aagttatggc gcgagctttc acactgtgtc ctcgtcctcc tcctccacga actcttccac
     1621 ttccacctcc tcgcctccga tctccatctt ggtggtcttc cggatgtcgc cgtggatcag
     1681 gatgtgctcg tggatcttga tcctcaggtg gtgggccagg aaggtccaaa actccttggc
     1741 gatgatgtcg cagatgctgt ggccgatgct gttggcgtaa gggctcagct tcagcagtcc
     1801 ttctggtccc actgcccaga ggattgggta gctgccccaa gaggcgaagt aagcgtaggc
     1861 catcagcttc acgaccatgc ggcaatggcc tttaggcacg ctgtggttgg cttccacgta
     1921 gcacttggcg gcctggaagt acatgtagcc gccgtagatg caagacacga tatacagcag
     1981 ccacttgagc caatcggtag ccagtcctgc ggccatgccg aacacgatca ttcccacgca
     2041 agacacgatc aggcccatgg tccgcttgct gtagtcgttc ttcaggccgc tcaggttgct
     2101 cagcttgatc aggatcacgg ggcaagacag cagccactcg aagtagcgca ggcaataggc
     2161 gtggttgccg gtagacaggt acactgtggc ggggctgctg aactccttga agatctccag
     2221 ggtcacgaac agcacctcga cgcaacagac gtagacctcc tcccaaccgc aagtggcctt
     2281 ccaggcgctg aagccgtaga atgtcagcag ggcgatggcg atgctgaaag caatccactg
     2341 gcagacctgg gcgccgatct tttctcctgg tgtgcctctg ctgtgcagcc aagcctgctt
     2401 cttctccact cccacgacca ggtagccgcc tgtggcctcc acgaagcaat atccgtatcc
     2461 ggcgtcgctc agtccgtaag aagggtcaca ccaccactcg gtgctgaagc actcgcctgt
     2521 aggtgtcatt cctccacagc ccatgtcctc gtggtggtag gggttaggcc aagggttgat
     2581 gcctccggcg gcaaacagag atctggtggc gctgctgatc agctcttgta cagctccctc
     2641 cggacgtccg gtgatctggg cctgtctaga gctgttttca gagatgttgt aggaaatctg
     2701 cagccctctt tccccataca catccggtct caaggttacc cccggtgact ttaactggtc
     2761 ctggtagtcg ggggtgtagg gatcaatggt ctgcatcagc acatagatgc acaaggcaaa
     2821 gagcccagtc atgaccacgt agaaagctgc atagtacagg ctgatccaca cccaccgggc
     2881 tggggtgcgg cccagcatct gcccagtgtc cgggttccaa cagtattgcc ggaattcggc
     2941 catgcgctgg ctgcatgact tcttctcctg cagggctgca ccggtataca gctcgtccat
     3001 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
     3061 gttggggtct ttgctcagct tggactgggt gctcaggtag tggttgtcgg gcagcagcac
     3121 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
     3181 ctcgatgttg cagttgaggc cgaagttggc cttgatgccg ttcttctgct tgtcggcggt
     3241 gatatagacg ttgccgtgga tggcgttgta ctccagcttg tgccccagga tgttgccgtc
     3301 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     3361 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
     3421 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
     3481 ggcgaagcac tgcacgcccc agctcagggt ggtcacgagg gtgggccagg gcacgggcag
     3541 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
     3601 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
     3661 caccaccccg gtgaacagct cctcgccctt gctcacgggt accgccacgt tgatgtcgat
     3721 ctggtccagg gggatgtact cgccctcgct ggtgatcctg ctcttggccg ccgtacgtcc
     3781 ccttccaaac attctgatat tgacttcgtc gttaggaggg gcattcttct cgtgattcag
     3841 gccaaaagga tcgtcggctt cgggtttctc ctgccctggc agacatgcgg ggtcccactt
     3901 cccttccagc actccccagg cagtgctcca caggatcact ccatatgtat ttttgcacag
     3961 gccgtcagcc agcaggtaca gaatggcgct cacgttctcg tggatcacgc agactccggt
     4021 gggtgaaatc agccacacga ttgggaacat agtccaagca ataaagaaga tcaggcgcag
     4081 catcttgatc ctcaccacca ccttctgtgc cagaggtgtc ccgacagatt caaagccata
     4141 cagccccact tgaaagattg tgaagaccac actgtactcg aacaccaggc aggtggcccc
     4201 aaagaaaaag aacagccact tcatatactc tgcgggagaa attgtagcgg tgattcccat
     4261 gaccactcta atgatggaag cggcctgtgc aatgggattc agtgggatac ttttgtactt
     4321 caccagagcc atgttagaaa tctgtcccag catgattgga caggtgcaca gccaggaggc
     4381 cattcggctc caaggaaaga atctgccgtc cctcatctgc agggttccgt tgccagtact
     4441 ggccagtgca taagtgatag attcgacaaa gggcaggtac acggactccc aagggacctt
     4501 gggagccctg ctaatcatga agattgtctg gcagcctgag accacggccc acaccagctg
     4561 cagcagtgag acgaaggtgg agtcgattct ggacatattg tagcactcgg tgtgagttga
     4621 tgcccattcc ccatagacga cctgtgatgc catggatcct agcataactt cgtataaagt
     4681 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
     4741 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcgat atcaagctta
     4801 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4861 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4921 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4981 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5041 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5101 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5161 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5221 tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5281 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5341 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5401 accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct
     5461 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
     5521 catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg
     5581 gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag
     5641 ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat
     5701 tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta
     5761 atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc
     5821 ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac
     5881 cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg
     5941 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     6001 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     6061 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     6121 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     6181 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6241 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6301 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6361 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6421 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6481 cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct
     6541 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6601 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6661 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6721 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     6781 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     6841 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6961 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7021 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7081 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7141 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7201 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7261 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7681 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8161 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8641 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
