LOCUS       pWPI-IRES-Bla-AK       12264 bp ss-DNA     circular SYN 12-JUN-2020
DEFINITION  pWPI lentiviral system.
ACCESSION   -
KEYWORDS    -.
SOURCE      
  ORGANISM  .
COMMENT     #497=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
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            #501=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
            #502=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
            #503=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
            #504=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)
            #505=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
            #506=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
            #507=(CWidgetStyle "Void" 0 0 0 0 0) #508=(CWidgetStyle "General
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            "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) (LOGSHAPE 1 1.9 0 0 0))
            #491=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1
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            0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
            1.05 0 1 48 "@F bp (molecule @L bp)" 0) #496=(CWidgetStyle
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            site") 1 5 "TCTAGA" "AGATCT") #476=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92086176 (8 0) 1 1 1 0 178) (CObjectList))
            "XhoI: 3 sites") 1 5 "CTCGAG" "GAGCTC") #477=(CRSFolderPar
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            #468=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085472 (8
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            #469=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085408 (8
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            "NTTAAN") #470=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
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            #483=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0
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            #484=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0
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            "RRE" "@N" 1 13.5098 5.22699 0 -10 1.98146 1.11561 #546#)
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            0.932452 Nil)) (CObjectList)) #526=(CGroupWidget (CWidget 10 (6 0)
            1 2 0 0 Nil -2538 100) (CObjectList #527=(CGroupWidget (CWidget 4
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            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
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            1.11561 #534#)) (CObjectList)) #536=(CGroupWidget (CWidget 9 (7 9
            0) 1 2 0 0 Nil -3061 100) (CObjectList #537=(CWideArrow (CWideLine
            (CWidget 0 (3 #114# 0) 1 2 0 0 #497# 99149004 100) (LOGPEN 0 0 0) 1
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            1 34 "Arial") 1.74233 0.666667 0 "ORF frame 3" "@N" 1 -16.0561
            -1.74233 0 -10 5.26169 1.11561 #530#) #532=(CWideArrow (CWideLine
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            3355545) 1 (LOGBRUSH 0 6724095 0) 1 0.188457 0.464163 1 0.082322)
            0.8 1.8 0) #533=(CLabel (CWidget 0 (0 0) 1 2 0 0 #482# 2136765432
            100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34
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            -10 5.26169 1.11561 #532#) #534=(CWideArrow (CObjectList
            #418=(CLinePar (CParagraph 0 (1 #64# 1) 1 2 2 0 191) " N1: 5844 "
            1))))) "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") #419=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92073792 (8 0) 1 1 1 0 178)
            (CObjectList)) "NlaIII: 32 sites") 5 1 "NCATGN" "NGTACN")
            #420=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073856 (8
            0) 1 1 1 0 178) (CObjectList #421=(CGroupPar (CParagraph 0 (10 #66#
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            1) 1 2 2 0 (CGroupPar (CParagraph 92064000 (8 0) 1 1 1 0 178)
            (CObjectList #413=(CGroupPar (CParagraph 0 (10 #62# 0) 1 2 2 0 180)
            (CObjectList #414=(CLinePar (CParagraph 0 (1 #62# 1) 1 2 2 0 191) "
            N1: 3517 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC")
            #415=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92060832 (8
            0) 1 1 1 0 178) (CObjectList)) "NgoMIV: 3 sites") 1 5 "GCCGGC"
            "CGGCCG") #416=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92064672 (8 0) 1 1 1 0 178) (CObjectList #417=(CGroupPar
            (CParagraph 0 (10 #64# 0) 1 2 2 0 180) (CParagraph 0 (1 #69# 1) 1 2
            2 0 191) " N1: 3322 " 1))))) "PacI: 1 site") 5 3 "TTAATTAA"
            "AATTAATT") #428=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92075680 (8 0) 1 1 1 0 178) (CObjectList)) "PciI: 4 sites") 1 5
            "ACATGT" "TGTACA") #429=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92076192 (8 0) 1 1 1 0 178) (CObjectList
            #430=(CGroupPar (CParagraph 0 (10 #71# 0) 1 2 2 0 180) (CObjectList
            #431=(CLinePar (CParagraph 0 (1 #71# 1) 1 2 2 0 191) " N1: 3491 "
            1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG")
            #432=(CRSFolderPar 191) " N1: 1146 " 1))))) "NotI: 1 site") 2 6
            "GCGGCCGC" "CGCCGGCG") #423=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92073920 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3
            sites") 3 3 "TCGCGA" "AGCGCT") #424=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92074816 (8 0) 1 1 1 0 178) (CObjectList))
            "NsiI: 2 sites") 5 1 "ATGCAT" "TACGTA") #425=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92078624 (8 0) 1 1 1 0 178)
            (CObjectList #426=(CGroupPar (CParagraph 0 (10 #69# 0) 1 2 2 0 180)
            (CObjectList #427=(CLinePar (CGroupPar (CParagraph 92549352 (8 0) 1
            1 1 0 178) (CObjectList)) "HpyCH4IV: 23 sites") 1 3 "ACGT" "TGCA")
            #401=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92549392 (8
            0) 1 1 1 0 178) (CObjectList)) "HpyCH4V: 51 sites") 2 2 "TGCA"
            "ACGT") #402=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92058368 (8 0) 1 1 1 0 178) (CObjectList)) "KasI: 5 sites") 1 5
            "GGCGCC" "CCGCGG") #403=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92056608 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 2
            sites") 5 1 "GGTACC" "CCATGG") #404=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058752 (8 0) 1 1 1 0 178) (CObjectList))
            "HinP1I: 45 sites") 1 3 "GCGC" "CGCG") #397=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92055008 (8 0) 1 1 1 0 178)
            (CObjectList)) "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG")
            #398=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055072 (8
            0) 1 1 1 0 178) (CObjectList)) "HpaII: 35 sites") 1 3 "CCGG"
            "GGCC") #399=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92055712 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4I: 32 sites") 5 1
            "NCATGN" "NGTACN") #400=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083872 (8 0) 1 1 1 0 178) (CObjectList)) "MseI: 55
            sites") 1 3 "TTAA" "AATT") #409=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058400 (8 0) 1 1 1 0 178) (CObjectList))
            "NaeI: 3 sites") 3 3 "GCCGGC" "CGGCCG") #410=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086784 (8 0) 1 1 1 0 178)
            (CObjectList)) "NarI: 5 sites") 2 4 "GGCGCC" "CCGCGG")
            #411=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92063808 (8
            0) 1 1 1 0 178) (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG"
            "GGTACC") #412=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92056800 (8 0) 1 1 1 0 178) (CObjectList)) "MboI: 50 sites") 1 5
            "NGATCN" "NCTAGN") #405=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92057888 (8 0) 1 1 1 0 178) (CObjectList)) "MfeI: 3
            sites") 1 5 "CAATTG" "GTTAAC") #406=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92057952 (8 0) 1 1 1 0 178) (CObjectList))
            "MluI: 0 sites") 1 5 "ACGCGT" "TGCGCA") #407=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92083904 (8 0) 1 1 1 0 178)
            (CObjectList)) "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT")
            #408=(CRSFolderPar (CFolderPar #454=(CGroupPar (CParagraph 0 (10
            #89# 0) 1 2 2 0 180) (CObjectList #455=(CLinePar (CParagraph 0 (1
            #89# 1) 1 2 2 0 191) " N1: 3531 " 1))))) "SnaBI: 1 site") 3 3
            "TACGTA" "ATGCAT") #456=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083936 (8 0) 1 1 1 0 178) (CObjectList
            #457=(CGroupPar (CParagraph 0 (10 #90# 0) 1 2 2 0 180) (CObjectList
            #458=(CLinePar (CParagraph 0 (1 #90# 1) 1 2 2 0 191) " N1: 3534 "
            1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #459=(CRSFolderPar
            #449=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084032 (8
            0) 1 1 1 0 178) (CObjectList)) "SfoI: 5 sites") 3 3 "GGCGCC"
            "CCGCGG") #450=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92084000 (8 0) 1 1 1 0 178) (CObjectList #451=(CGroupPar
            (CParagraph 0 (10 #88# 0) 1 2 2 0 180) (CObjectList #452=(CLinePar
            (CParagraph 0 (1 #88# 1) 1 2 2 0 191) " N1: 3497 " 1))))) "SmaI: 1
            site") 3 3 "CCCGGG" "GGGCCC") #453=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92083968 (8 0) 1 1 1 0 178) (CObjectList
            (CFolderPar (CGroupPar (CParagraph 92085920 (8 0) 1 1 1 0 178)
            (CObjectList)) "StuI: 3 sites") 3 3 "AGGCCT" "TCCGGA")
            #464=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085856 (8
            0) 1 1 1 0 178) (CObjectList #465=(CGroupPar (CParagraph 0 (10 #96#
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            (CWidget 0 (3 #120# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 3.01299 1.9 0.082322 1) #565=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 63286324 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "Sp6 primer" "@N" 1 -5.00421
            -15.681 0 -10 4.62896 1.11561 #564#) #566=(CScratch (CWidget 0 (3
            #121# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507) 10 1 2.83442
            1.9 0.082322 1) #567=(CLabel (CWideLine (CWidget 0 (3 #118# 0) 1 2
            0 0 #486# 99148984 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334
            0) 0.835356 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #561=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 1371 100) (LOGPEN 0 0 0) 1 (LOGFONT
            69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "truncHIV-1 3 LTR" "@N" 1 -5.56886 -20.908 0 -10 7.14325 1.11561
            #560#) #562=(CWideArrow (CWideLine (CWidget 0 (3 #119# 0) 1 2 0 0
            #486# 63308488 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0)
            0.670712 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #563=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (CObjectList
            #601=(CLine (CWidget 0 (3 #136# 0) 1 2 0 0 #490# 1834 100) (LOGPEN
            0 12 13395507) 10 0.835356 0.0971369 0.173916) #602=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 59796852 100) (LOGPEN 0 0 0) 1
            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "SV40 PA terminator" "@N" 1 -7.08378 20.908 0 -10 8.20891 1.11561
            #601#)) (CObjectList))) (CObjectList)) #603=(CGroupWidget (CWidget
            11 (8 0) 1 2 0 0 Nil -2119 100) (CObjectList #604=(CGroupWidget
            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1805 100) (CObjectList
            #605=(CScratch 0.082322) 0.8 1.8 0) #597=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "pBR322 origin" "@N" 1
            -16.4307 1.74233 0 -10 5.97768 1.11561 #596#) #598=(CScratch
            (CWidget 0 (3 #129# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 1.29419 1.9 0.082322 1) #599=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 59795284 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "SV40 origin" "@N" 1 -15.7111
            6.96932 0 -10 4.86207 1.11561 #598#)) (CObjectList))
            #600=(CGroupWidget (CWidget 43 (7 43 0) 1 2 0 0 Nil -2643 100)
            (LOGPEN 0 0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18
            "Georgia") 1.74233 0.555556 128 "{\\i Asc}I (3500)" "@N (@S)" 5
            15.2669 -15.681 0 -10 4.59566 0.932452 #608#)) (CObjectList))
            #610=(CGroupWidget.
FEATURES             Location/Qualifiers
     source          1..12264
                     /mol_type="other DNA"
                     /note="/vntifkey=""""98"""""
                     /organism="pWPI"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    746..790
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_psi_pack"
     CDS             1178..2035
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\2"
     misc_feature    1300..1533
                     /note="/vntifkey=""""21"""""
                     /label="RRE"
     promoter        2127..3297
                     /note="/vntifkey=""""30"""""
                     /label="EF1a_promoter"
     misc_feature    3349..3364
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     polylinker      3494..3499
                     /created_by="pbeare"
                     /modified_by="pbeare"
                     /label="SmaI/XmaI site"
     misc_feature    3499^3500
                     /Original_Bases="AGCGCCACCATGTCAAGCTCTTCCTGGCTCCTTCTCAGCCT
                     TGTTGCTGTAACTGCTGCTCAGTCCACCATTGAGGAACAGGCCAAGACATTTTTGGAC
                     AAGTTTAACCACGAAGCCGAAGACCTGTTCTATCAAAGTTCACTTGCTTCTTGGAATT
                     ATAACACCAATATTACTGAAGAGAATGTCCAAAACATGAATAATGCTGGGGACAAATG
                     GTCTGCCTTTTTAAAGGAACAGTCCACACTTGCCCAAATGTATCCACTACAAGAAATT
                     CAGAATCTCACAGTCAAGCTTCAGCTGCAGGCTCTTCAGCAAAATGGGTCTTCAGTGC
                     TCTCAGAAGACAAGAGCAAACGGTTGAACACAATTCTAAATACAATGAGCACCATCTA
                     CAGTACTGGAAAAGTTTGTAACCCAGATAATCCACAAGAATGCTTATTACTTGAACCA
                     GGTTTGAATGAAATAATGGCAAACAGTTTAGACTACAATGAGAGGCTCTGGGCTTGGG
                     AAAGCTGGAGATCTGAGGTCGGCAAGCAGCTGAGGCCATTATATGAAGAGTATGTGGT
                     CTTGAAAAATGAGATGGCAAGAGCAAATCATTATGAGGACTATGGGGATTATTGGAGA
                     GGAGACTATGAAGTAAATGGGGTAGATGGCTATGACTACAGCCGCGGCCAGTTGATTG
                     AAGATGTGGAACATACCTTTGAAGAGATTAAACCATTATATGAACATCTTCATGCCTA
                     TGTGAGGGCAAAGTTGATGAATGCCTATCCTTCCTATATCAGTCCAATTGGATGCCTC
                     CCTGCTCATTTGCTTGGTGATATGTGGGGTAGATTTTGGACAAATCTGTACTCTTTGA
                     CAGTTCCCTTTGGACAGAAACCAAACATAGATGTTACTGATGCAATGGTGGACCAGGC
                     CTGGGATGCACAGAGAATATTCAAGGAGGCCGAGAAGTTCTTTGTATCTGTTGGTCTT
                     CCTAATATGACTCAAGGATTCTGGGAAAATTCCATGCTAACGGACCCAGGAAATGTTC
                     AGAAAGCAGTCTGCCATCCCACAGCTTGGGACCTGGGGAAGGGCGACTTCAGGATCCT
                     TATGTGCACAAAGGTGACAATGGACGACTTCCTGACAGCTCATCATGAGATGGGGCAT
                     ATCCAGTATGATATGGCATATGCTGCACAACCTTTTCTGCTAAGAAATGGAGCTAATG
                     AAGGATTCCATGAAGCTGTTGGGGAAATCATGTCACTTTCTGCAGCCACACCTAAGCA
                     TTTAAAATCCATTGGTCTTCTGTCACCCGATTTTCAAGAAGACAATGAAACAGAAATA
                     AACTTCCTGCTCAAACAAGCACTCACGATTGTTGGGACTCTGCCATTTACTTACATGT
                     TAGAGAAGTGGAGGTGGATGGTCTTTAAAGGGGAAATTCCCAAAGACCAGTGGATGAA
                     AAAGTGGTGGGAGATGAAGCGAGAGATAGTTGGGGTGGTGGAACCTGTGCCCCATGAT
                     GAAACATACTGTGACCCCGCATCTCTGTTCCATGTTTCTAATGATTACTCATTCATTC
                     GATATTACACAAGGACCCTTTACCAATTCCAGTTTCAAGAAGCACTTTGTCAAGCAGC
                     TAAACATGAAGGCCCTCTGCACAAATGTGACATCTCAAACTCTACAGAAGCTGGACAG
                     AAACTGTTCAATATGCTGAGGCTTGGAAAATCAGAACCCTGGACCCTAGCATTGGAAA
                     ATGTTGTAGGAGCAAAGAACATGAATGTAAGGCCACTGCTCAACTACTTTGAGCCCTT
                     ATTTACCTGGCTGAAAGACCAGAACAAGAATTCTTTTGTGGGATGGAGTACCGACTGG
                     AGTCCATATGCAGACCAAAGCATCAAAGTGAGGATAAGCCTAAAATCAGCTCTTGGAG
                     ATAAAGCATATGAATGGAACGACAATGAAATGTACCTGTTCCGATCATCTGTTGCATA
                     TGCTATGAGGCAGTACTTTTTAAAAGTAAAAAATCAGATGATTCTTTTTGGGGAGGAG
                     GATGTGCGAGTGGCTAATTTGAAACCAAGAATCTCCTTTAATTTCTTTGTCACTGCAC
                     CTAAAAATGTGTCTGATATCATTCCTAGAACTGAAGTTGAAAAGGCCATCAGGATGTC
                     CCGGAGCCGTATCAATGATGCTTTCCGTCTGAATGACAACAGCCTAGAGTTTCTGGGG
                     ATACAGCCAACACTTGGACCTCCTAACCAGCCCCCTGTTTCCATATGGCTGATTGTTT
                     TTGGAGTTGTGATGGGAGTGATAGTGGTTGGCATTGTCATCCTGATCTTCACTGGGAT
                     CAGAGATCGGAAGAAGAAAAATAAAGCAAGAAGTGGAGAAAATCCTTATGCCTCCATC
                     GATATTAGCAAAGGAGAAAATAATCCAGGATTCCAAAACACTGATGATGTTCAGACCT
                     CCTTTTAG"
                     /label="AGCGCCACCATGTCAAGCTCTTCCTGGCTCCTTCTCAGCCTTGTTGCTGT
                     AACTGCTGCTCAGTCCACCATTGAGGAACAGGCCAAGACATTTTTGGACAAGTTTAAC
                     CACGAAGCCGAAGACCTGTTCTATCAAAGTTCACTTGCTTCTTGGAATTATAACACCA
                     ATATTACTGAAGAGAATGTCCAAAACATGAATAATGCTGGGGACAAATGGTCTGCCTT
                     TTTAAAGGAACAGTCCACACTTGCCCAAATGTATCCACTACAAGAAATTCAGAATCTC
                     ACAGTCAAGCTTCAGCTGCAGGCTCTTCAGCAAAATGGGTCTTCAGTGCTCTCAGAAG
                     ACAAGAGCAAACGGTTGAACACAATTCTAAATACAATGAGCACCATCTACAGTACTGG
                     AAAAGTTTGTAACCCAGATAATCCACAAGAATGCTTATTACTTGAACCAGGTTTGAAT
                     GAAATAATGGCAAACAGTTTAGACTACAATGAGAGGCTCTGGGCTTGGGAAAGCTGGA
                     GATCTGAGGTCGGCAAGCAGCTGAGGCCATTATATGAAGAGTATGTGGTCTTGAAAAA
                     TGAGATGGCAAGAGCAAATCATTATGAGGACTATGGGGATTATTGGAGAGGAGACTAT
                     GAAGTAAATGGGGTAGATGGCTATGACTACAGCCGCGGCCAGTTGATTGAAGATGTGG
                     AACATACCTTTGAAGAGATTAAACCATTATATGAACATCTTCATGCCTATGTGAGGGC
                     AAAGTTGATGAATGCCTATCCTTCCTATATCAGTCCAATTGGATGCCTCCCTGCTCAT
                     TTGCTTGGTGATATGTGGGGTAGATTTTGGACAAATCTGTACTCTTTGACAGTTCCCT
                     TTGGACAGAAACCAAACATAGATGTTACTGATGCAATGGTGGACCAGGCCTGGGATGC
                     ACAGAGAATATTCAAGGAGGCCGAGAAGTTCTTTGTATCTGTTGGTCTTCCTAATATG
                     ACTCAAGGATTCTGGGAAAATTCCATGCTAACGGACCCAGGAAATGTTCAGAAAGCAG
                     TCTGCCATCCCACAGCTTGGGACCTGGGGAAGGGCGACTTCAGGATCCTTATGTGCAC
                     AAAGGTGACAATGGACGACTTCCTGACAGCTCATCATGAGATGGGGCATATCCAGTAT
                     GATATGGCATATGCTGCACAACCTTTTCTGCTAAGAAATGGAGCTAATGAAGGATTCC
                     ATGAAGCTGTTGGGGAAATCATGTCACTTTCTGCAGCCACACCTAAGCATTTAAAATC
                     CATTGGTCTTCTGTCACCCGATTTTCAAGAAGACAATGAAACAGAAATAAACTTCCTG
                     CTCAAACAAGCACTCACGATTGTTGGGACTCTGCCATTTACTTACATGTTAGAGAAGT
                     GGAGGTGGATGGTCTTTAAAGGGGAAATTCCCAAAGACCAGTGGATGAAAAAGTGGTG
                     GGAGATGAAGCGAGAGATAGTTGGGGTGGTGGAACCTGTGCCCCATGATGAAACATAC
                     TGTGACCCCGCATCTCTGTTCCATGTTTCTAATGATTACTCATTCATTCGATATTACA
                     CAAGGACCCTTTACCAATTCCAGTTTCAAGAAGCACTTTGTCAAGCAGCTAAACATGA
                     AGGCCCTCTGCACAAATGTGACATCTCAAACTCTACAGAAGCTGGACAGAAACTGTTC
                     AATATGCTGAGGCTTGGAAAATCAGAACCCTGGACCCTAGCATTGGAAAATGTTGTAG
                     GAGCAAAGAACATGAATGTAAGGCCACTGCTCAACTACTTTGAGCCCTTATTTACCTG
                     GCTGAAAGACCAGAACAAGAATTCTTTTGTGGGATGGAGTACCGACTGGAGTCCATAT
                     GCAGACCAAAGCATCAAAGTGAGGATAAGCCTAAAATCAGCTCTTGGAGATAAAGCAT
                     ATGAATGGAACGACAATGAAATGTACCTGTTCCGATCATCTGTTGCATATGCTATGAG
                     GCAGTACTTTTTAAAAGTAAAAAATCAGATGATTCTTTTTGGGGAGGAGGATGTGCGA
                     GTGGCTAATTTGAAACCAAGAATCTCCTTTAATTTCTTTGTCACTGCACCTAAAAATG
                     TGTCTGATATCATTCCTAGAACTGAAGTTGAAAAGGCCATCAGGATGTCCCGGAGCCG
                     TATCAATGATGCTTTCCGTCTGAATGACAACAGCCTAGAGTTTCTGGGGATACAGCCA
                     ACACTTGGACCTCCTAACCAGCCCCCTGTTTCCATATGGCTGATTGTTTTTGGAGTTG
                     TGATGGGAGTGATAGTGGTTGGCATTGTCATCCTGATCTTCACTGGGATCAGAGATCG
                     GAAGAAGAAAAATAAAGCAAGAAGTGGAGAAAATCCTTATGCCTCCATCGATATTAGC
                     AAAGGAGAAAATAATCCAGGATTCCAAAACACTGATGATGTTCAGACCTCCTTTTAG"
                     /note="Geneious type: Editing History Deletion"
     source          <3500..>3509
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     Kozak           3501..3509
                     /created_by="pbeare"
                     /label="Kozak sequence"
     CDS             3510..4988
                     /created_by="pbeare"
                     /modified_by="pbeare"
                     /label="TMPRSS2"
     misc_feature    5055..5617
                     /note="/vntifkey=""""21"""""
                     /label="IRES"
     CDS             5621..6019
                     /note="/vntifkey=""""4"""""
                     /label="blasticidin\resistance"
     misc_feature    complement(6065..6081)
                     /note="/vntifkey=""""21"""""
                     /label="pBluescriptKS_primer"
     enhancer        6096..6688
                     /note="/vntifkey=""""9"""""
                     /label="WPRE"
     misc_feature    6754..6775
                     /note="/vntifkey=""""21"""""
                     /label="U3PPT"
     misc_feature    6754..6769
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    6826..6859
                     /note="/vntifkey=""""21"""""
                     /label="loxP"
     misc_feature    6879..7059
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    6879..7059
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    complement(7085..7102)
                     /note="/vntifkey=""""21"""""
                     /label="Sp6_primer"
     gene            7340..7603
                     /gene="tet (300 - 563)"
                     /note="/vntifkey=""""60"""""
     misc_feature    complement(7392..7411)
                     /note="/vntifkey=""""21"""""
                     /label="pBRrevBam_primer"
     misc_feature    7744..7766
                     /note="/vntifkey=""""21"""""
                     /label="pGEX_3_primer"
     promoter        7925..7953
                     /note="/vntifkey=""""30"""""
                     /label="AmpR_promoter"
     gene            7995..8855
                     /gene="Ampicillin"
                     /note="/vntifkey=""""60"""""
     CDS             7995..8855
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\3"
     rep_origin      9010..9629
                     /note="/vntifkey=""""33"""""
                     /label="pBR322_origin"
     misc_feature    complement(9855..9875)
                     /note="/vntifkey=""""21"""""
                     /label="pBABE_3_primer"
     misc_feature    complement(9861..10314)
                     /note="/vntifkey=""""21"""""
                     /label="SV40_enhancer"
     promoter        9873..10141
                     /note="/vntifkey=""""30"""""
                     /label="SV40_promoter"
     rep_origin      10040..10117
                     /note="/vntifkey=""""33"""""
                     /label="SV40_origin"
     misc_feature    10102..10121
                     /note="/vntifkey=""""21"""""
                     /label="SV40pro_F_primer"
     misc_feature    11322..11337
                     /note="/vntifkey=""""21"""""
                     /label="SV40_int"
     misc_feature    11343..11390
                     /note="/vntifkey=""""21"""""
                     /label="SV40_3_splice"
     CDS             11467..11940
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\1"
     misc_feature    11518..11538
                     /note="/vntifkey=""""21"""""
                     /label="SV40\NLS"
     misc_feature    11524..11538
                     /note="/vntifkey=""""21"""""
                     /label="NLS"
     terminator      11966..12097
                     /note="/vntifkey=""""43"""""
                     /label="SV40_PA_terminator"
     misc_feature    12054..12073
                     /note="/vntifkey=""""21"""""
                     /label="EBV_rev_primer"
ORIGIN      
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacccggga gcaggcacca tggctttgaa ctcagggtca ccaccagcta
     3541 ttggacctta ctatgaaaac catggatacc aaccggaaaa cccctatccc gcacagccca
     3601 ctgtggtccc cactgtctac gaggtgcatc cggctcagta ctacccgtcc cccgtgcccc
     3661 agtacgcccc gagggtcctg acgcaggctt ccaaccccgt cgtctgcacg cagcccaaat
     3721 ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc actgtgcatc accttgaccc
     3781 tggggacctt cctcgtggga gctgcgctgg ccgctggcct actctggaag ttcatgggca
     3841 gcaagtgctc caactctggg atagagtgcg actcctcagg tacctgcatc aacccctcta
     3901 actggtgtga tggcgtgtca cactgccccg gcggggagga cgagaatcgg tgtgttcgcc
     3961 tctacggacc aaacttcatc cttcaggtgt actcatctca gaggaagtcc tggcaccctg
     4021 tgtgccaaga cgactggaac gagaactacg ggcgggcggc ctgcagggac atgggctata
     4081 agaataattt ttactctagc caaggaatag tggatgacag cggatccacc agctttatga
     4141 aactgaacac aagtgccggc aatgtcgata tctataaaaa actgtaccac agtgatgcct
     4201 gttcttcaaa agcagtggtt tctttacgct gtatagcctg cggggtcaac ttgaactcaa
     4261 gccgccagag caggatcgtg ggcggcgaga gcgcgctccc gggggcctgg ccctggcagg
     4321 tcagcctgca cgtccagaac gtccacgtgt gcggaggctc catcatcacc cccgagtgga
     4381 tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa tccatggcat tggacggcat
     4441 ttgcggggat tttgagacaa tctttcatgt tctatggagc cggataccaa gtagaaaaag
     4501 tgatttctca tccaaattat gactccaaga ccaagaacaa tgacattgcg ctgatgaagc
     4561 tgcagaagcc tctgactttc aacgacctag tgaaaccagt gtgtctgccc aacccaggca
     4621 tgatgctgca gccagaacag ctctgctgga tttccgggtg gggggccacc gaggagaaag
     4681 ggaagacctc agaagtgctg aacgctgcca aggtgcttct cattgagaca cagagatgca
     4741 acagcagata tgtctatgac aacctgatca caccagccat gatctgtgcc ggcttcctgc
     4801 aggggaacgt cgattcttgc cagggtgaca gtggagggcc tctggtcact tcgaagaaca
     4861 atatctggtg gctgataggg gatacaagct ggggttctgg ctgtgccaaa gcttacagac
     4921 caggagtgta cgggaatgtg atggtattca cggactggat ttatcgacaa atgagggcag
     4981 acggctaggg atccatatgc ggaccgtacg tactagtggc cacggctgat catacgggct
     5041 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     5581 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggccaagc ctttgtctca
     5641 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     5701 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     5761 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     5821 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     5881 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     5941 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     6001 ttatgtgtgg gagggctaac tagctagtcg agctcaactt cgaattcgat atcaagctta
     6061 tcgcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     6121 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6181 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6241 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6301 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6361 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6421 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6481 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6541 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6601 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6661 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac
     6721 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6781 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     6841 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6901 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6961 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7021 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     7081 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     7141 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     7201 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     7261 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     7321 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     7381 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     7441 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     7501 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     7561 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     7621 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7681 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7741 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7801 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7861 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7921 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7981 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8041 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8101 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8161 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8221 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8281 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8341 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8401 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8461 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8521 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8581 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8641 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8701 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8761 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8821 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8881 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8941 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     9181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     9421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9841 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9961 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10081 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10141 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    10201 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    10261 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    10321 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    10381 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    10441 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    10501 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10561 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10621 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    10681 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    10741 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    10801 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    10861 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    10921 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    10981 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    11041 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    11101 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    11161 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    11221 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    11281 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    11341 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    11401 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    11461 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    11521 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11581 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    11641 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    11701 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    11761 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    11821 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    11881 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11941 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    12001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    12061 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    12121 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    12181 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12241 aaatatgtac tacaaactta gtag
//
