LOCUS       pWPI-IRES-Puro-A       10983 bp ss-DNA     circular SYN 12-JUN-2020
DEFINITION  pWPI lentiviral system.
ACCESSION   -
KEYWORDS    -.
SOURCE      
  ORGANISM  .
COMMENT     #497=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
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            #501=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
            #502=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
            #503=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
            #504=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)
            #505=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
            #506=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
            #507=(CWidgetStyle "Void" 0 0 0 0 0) #508=(CWidgetStyle "General
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            "Times New Roman") 0.91 0 1 3 "@T @N " 0) #509=(CWidgetStyle
            "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) (LOGSHAPE 1 1.9 0 0 0))
            #491=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1
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            0) #494=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1
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            65535 "@N (@H)" 0) #495=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN
            0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
            1.05 0 1 48 "@F bp (molecule @L bp)" 0) #496=(CWidgetStyle
            "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0
            0 0 3 2 1 34 "Arial") 0.91 0 1 1 "Fragment of @N" 0) (LOGSHAPE 9 1
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            0.164644 1.74233 0.164644 2.53336 (70 (CShapeMapEntry 0 "Unique
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            "GGGCCC") #473=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
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            site") 1 5 "TCTAGA" "AGATCT") #476=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92086176 (8 0) 1 1 1 0 178) (CObjectList))
            "XhoI: 3 sites") 1 5 "CTCGAG" "GAGCTC") #477=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086112 (8 0) 1 1 1 0 178)
            #468=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085472 (8
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            #469=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085408 (8
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            "NTTAAN") #470=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
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            #483=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0
            0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 "@L bp" 0)
            #484=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0
            0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 "@N" 0) #485=(CWidgetStyle
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            #489=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1
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            (1 #103# 1) 1 2 2 0 191) " N1: 3495 " 1))))) "XmaI: 1 site") 1 5
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            #481=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1
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            "RRE" "@N" 1 13.5098 5.22699 0 -10 1.98146 1.11561 #546#)
            #528=(CWideArrow (CWideLine (CWidget 0 (3 #108# 0) 1 2 0 0 #485# 0
            100) (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 1 5.08951
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            0 #482# 268889064 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0
            0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "ORF frame 2" "@N" 1
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            (LOGFONT 87 33 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 1.74233 0.833333
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            0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233
            0.555556 0 "10785 bp" "@L bp" 16 0 -0.871165 0 -10.8 3.5966
            0.932452 Nil)) (CObjectList)) #526=(CGroupWidget (CWidget 10 (6 0)
            1 2 0 0 Nil -2538 100) (CObjectList #527=(CGroupWidget (CWidget 4
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            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "blasticidin resistance" "@N" 1 13.4697 -31.3619 0 -10 8.79169
            1.11561 #534#)) (CObjectList)) #536=(CGroupWidget (CWidget 9 (7 9
            0) 1 2 0 0 Nil -3061 100) (CObjectList #537=(CWideArrow (CWideLine
            (CWidget 0 (3 #114# 0) 1 2 0 0 #497# 99149004 100) (LOGPEN 0 0 0) 1
            (LOGBRUSH 2 8388608 5) 1 3.24333 3.58825 1 (CWidget 0 (0 0) 1 2 0 0
            #482# 2188 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2
            1 34 "Arial") 1.74233 0.666667 0 "ORF frame 3" "@N" 1 -16.0561
            -1.74233 0 -10 5.26169 1.11561 #530#) #532=(CWideArrow (CWideLine
            (CWidget 0 (3 #133# 0) 1 2 0 0 #485# 62653014 100) (LOGPEN 0 0
            3355545) 1 (LOGBRUSH 0 6724095 0) 1 0.188457 0.464163 1 0.082322)
            0.8 1.8 0) #533=(CLabel (CWidget 0 (0 0) 1 2 0 0 #482# 2136765432
            100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34
            "Arial") 1.74233 0.666667 0 "ORF frame 1" "@N" 1 -8.04783 19.1656 0
            -10 5.26169 1.11561 #532#) #534=(CWideArrow (CObjectList
            #418=(CLinePar (CParagraph 0 (1 #64# 1) 1 2 2 0 191) " N1: 5844 "
            1))))) "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") #419=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92073792 (8 0) 1 1 1 0 178)
            (CObjectList)) "NlaIII: 32 sites") 5 1 "NCATGN" "NGTACN")
            #420=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073856 (8
            0) 1 1 1 0 178) (CObjectList #421=(CGroupPar (CParagraph 0 (10 #66#
            0) 1 2 2 0 180) (CObjectList #422=(CLinePar (CParagraph 0 (1 #66#
            1) 1 2 2 0 (CGroupPar (CParagraph 92064000 (8 0) 1 1 1 0 178)
            (CObjectList #413=(CGroupPar (CParagraph 0 (10 #62# 0) 1 2 2 0 180)
            (CObjectList #414=(CLinePar (CParagraph 0 (1 #62# 1) 1 2 2 0 191) "
            N1: 3517 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC")
            #415=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92060832 (8
            0) 1 1 1 0 178) (CObjectList)) "NgoMIV: 3 sites") 1 5 "GCCGGC"
            "CGGCCG") #416=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92064672 (8 0) 1 1 1 0 178) (CObjectList #417=(CGroupPar
            (CParagraph 0 (10 #64# 0) 1 2 2 0 180) (CParagraph 0 (1 #69# 1) 1 2
            2 0 191) " N1: 3322 " 1))))) "PacI: 1 site") 5 3 "TTAATTAA"
            "AATTAATT") #428=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92075680 (8 0) 1 1 1 0 178) (CObjectList)) "PciI: 4 sites") 1 5
            "ACATGT" "TGTACA") #429=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92076192 (8 0) 1 1 1 0 178) (CObjectList
            #430=(CGroupPar (CParagraph 0 (10 #71# 0) 1 2 2 0 180) (CObjectList
            #431=(CLinePar (CParagraph 0 (1 #71# 1) 1 2 2 0 191) " N1: 3491 "
            1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG")
            #432=(CRSFolderPar 191) " N1: 1146 " 1))))) "NotI: 1 site") 2 6
            "GCGGCCGC" "CGCCGGCG") #423=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92073920 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3
            sites") 3 3 "TCGCGA" "AGCGCT") #424=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92074816 (8 0) 1 1 1 0 178) (CObjectList))
            "NsiI: 2 sites") 5 1 "ATGCAT" "TACGTA") #425=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92078624 (8 0) 1 1 1 0 178)
            (CObjectList #426=(CGroupPar (CParagraph 0 (10 #69# 0) 1 2 2 0 180)
            (CObjectList #427=(CLinePar (CGroupPar (CParagraph 92549352 (8 0) 1
            1 1 0 178) (CObjectList)) "HpyCH4IV: 23 sites") 1 3 "ACGT" "TGCA")
            #401=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92549392 (8
            0) 1 1 1 0 178) (CObjectList)) "HpyCH4V: 51 sites") 2 2 "TGCA"
            "ACGT") #402=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92058368 (8 0) 1 1 1 0 178) (CObjectList)) "KasI: 5 sites") 1 5
            "GGCGCC" "CCGCGG") #403=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92056608 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 2
            sites") 5 1 "GGTACC" "CCATGG") #404=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058752 (8 0) 1 1 1 0 178) (CObjectList))
            "HinP1I: 45 sites") 1 3 "GCGC" "CGCG") #397=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92055008 (8 0) 1 1 1 0 178)
            (CObjectList)) "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG")
            #398=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055072 (8
            0) 1 1 1 0 178) (CObjectList)) "HpaII: 35 sites") 1 3 "CCGG"
            "GGCC") #399=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92055712 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4I: 32 sites") 5 1
            "NCATGN" "NGTACN") #400=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083872 (8 0) 1 1 1 0 178) (CObjectList)) "MseI: 55
            sites") 1 3 "TTAA" "AATT") #409=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058400 (8 0) 1 1 1 0 178) (CObjectList))
            "NaeI: 3 sites") 3 3 "GCCGGC" "CGGCCG") #410=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086784 (8 0) 1 1 1 0 178)
            (CObjectList)) "NarI: 5 sites") 2 4 "GGCGCC" "CCGCGG")
            #411=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92063808 (8
            0) 1 1 1 0 178) (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG"
            "GGTACC") #412=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92056800 (8 0) 1 1 1 0 178) (CObjectList)) "MboI: 50 sites") 1 5
            "NGATCN" "NCTAGN") #405=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92057888 (8 0) 1 1 1 0 178) (CObjectList)) "MfeI: 3
            sites") 1 5 "CAATTG" "GTTAAC") #406=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92057952 (8 0) 1 1 1 0 178) (CObjectList))
            "MluI: 0 sites") 1 5 "ACGCGT" "TGCGCA") #407=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92083904 (8 0) 1 1 1 0 178)
            (CObjectList)) "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT")
            #408=(CRSFolderPar (CFolderPar #454=(CGroupPar (CParagraph 0 (10
            #89# 0) 1 2 2 0 180) (CObjectList #455=(CLinePar (CParagraph 0 (1
            #89# 1) 1 2 2 0 191) " N1: 3531 " 1))))) "SnaBI: 1 site") 3 3
            "TACGTA" "ATGCAT") #456=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083936 (8 0) 1 1 1 0 178) (CObjectList
            #457=(CGroupPar (CParagraph 0 (10 #90# 0) 1 2 2 0 180) (CObjectList
            #458=(CLinePar (CParagraph 0 (1 #90# 1) 1 2 2 0 191) " N1: 3534 "
            1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #459=(CRSFolderPar
            #449=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084032 (8
            0) 1 1 1 0 178) (CObjectList)) "SfoI: 5 sites") 3 3 "GGCGCC"
            "CCGCGG") #450=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92084000 (8 0) 1 1 1 0 178) (CObjectList #451=(CGroupPar
            (CParagraph 0 (10 #88# 0) 1 2 2 0 180) (CObjectList #452=(CLinePar
            (CParagraph 0 (1 #88# 1) 1 2 2 0 191) " N1: 3497 " 1))))) "SmaI: 1
            site") 3 3 "CCCGGG" "GGGCCC") #453=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92083968 (8 0) 1 1 1 0 178) (CObjectList
            (CFolderPar (CGroupPar (CParagraph 92085920 (8 0) 1 1 1 0 178)
            (CObjectList)) "StuI: 3 sites") 3 3 "AGGCCT" "TCCGGA")
            #464=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085856 (8
            0) 1 1 1 0 178) (CObjectList #465=(CGroupPar (CParagraph 0 (10 #96#
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            (CWidget 0 (3 #120# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 3.01299 1.9 0.082322 1) #565=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 63286324 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "Sp6 primer" "@N" 1 -5.00421
            -15.681 0 -10 4.62896 1.11561 #564#) #566=(CScratch (CWidget 0 (3
            #121# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507) 10 1 2.83442
            1.9 0.082322 1) #567=(CLabel (CWideLine (CWidget 0 (3 #118# 0) 1 2
            0 0 #486# 99148984 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334
            0) 0.835356 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #561=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 1371 100) (LOGPEN 0 0 0) 1 (LOGFONT
            69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "truncHIV-1 3 LTR" "@N" 1 -5.56886 -20.908 0 -10 7.14325 1.11561
            #560#) #562=(CWideArrow (CWideLine (CWidget 0 (3 #119# 0) 1 2 0 0
            #486# 63308488 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0)
            0.670712 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #563=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (CObjectList
            #601=(CLine (CWidget 0 (3 #136# 0) 1 2 0 0 #490# 1834 100) (LOGPEN
            0 12 13395507) 10 0.835356 0.0971369 0.173916) #602=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 59796852 100) (LOGPEN 0 0 0) 1
            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "SV40 PA terminator" "@N" 1 -7.08378 20.908 0 -10 8.20891 1.11561
            #601#)) (CObjectList))) (CObjectList)) #603=(CGroupWidget (CWidget
            11 (8 0) 1 2 0 0 Nil -2119 100) (CObjectList #604=(CGroupWidget
            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1805 100) (CObjectList
            #605=(CScratch 0.082322) 0.8 1.8 0) #597=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "pBR322 origin" "@N" 1
            -16.4307 1.74233 0 -10 5.97768 1.11561 #596#) #598=(CScratch
            (CWidget 0 (3 #129# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 1.29419 1.9 0.082322 1) #599=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 59795284 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "SV40 origin" "@N" 1 -15.7111
            6.96932 0 -10 4.86207 1.11561 #598#)) (CObjectList))
            #600=(CGroupWidget (CWidget 43 (7 43 0) 1 2 0 0 Nil -2643 100)
            (LOGPEN 0 0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18
            "Georgia") 1.74233 0.555556 128 "{\\i Asc}I (3500)" "@N (@S)" 5
            15.2669 -15.681 0 -10 4.59566 0.932452 #608#)) (CObjectList))
            #610=(CGroupWidget.
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="other DNA"
                     /note="/vntifkey=""""98"""""
                     /organism="pWPI"
     misc_feature    309..314
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    746..790
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_psi_pack"
     misc_feature    1144..1151
                     /Recognition_pattern="GC^GGCCGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     CDS             1178..2035
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\2"
     misc_feature    1300..1533
                     /note="/vntifkey=""""21"""""
                     /label="RRE"
     misc_feature    1570..1579
                     /Recognition_pattern="CACNN^NNGTG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACNNNNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    1930..1936
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     promoter        2127..3297
                     /note="/vntifkey=""""30"""""
                     /label="EF1a_promoter"
     misc_feature    2818..2825
                     /Recognition_pattern="GGCCGG^CC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    2866..2872
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    3309..3316
                     /Recognition_pattern="ATTT^AAAT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATTTAAAT"
                     /label="SwaI, SmiI"
                     /note="Geneious type: restriction site"
     misc_feature    3317..3324
                     /Recognition_pattern="TTAAT^TAA"
                     /Methylation="EcoKI:cut"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     misc_feature    3349..3364
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    3487..3553
                     /note="/vntifkey=""""21"""""
                     /label="MCS"
     misc_feature    3487..3494
                     /Recognition_pattern="GTTT^AAAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     misc_feature    3494..3499
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3494..3499
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3498..3505
                     /Recognition_pattern="GG^CGCGCC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     misc_feature    3504..3511
                     /Recognition_pattern="CCTGCA^GG"
                     /annotation_group="CCTGCAGG"
                     /label="Sse8387I, SbfI, SdaI"
                     /note="Geneious type: restriction site"
     misc_feature    3510..3515
                     /Recognition_pattern="G^GATCC"
                     /Methylation="Dam:cut, Dcm:cut, CpG:cut"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    3515..3520
                     /Recognition_pattern="CA^TATG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    3521..3527
                     /Recognition_pattern="CG^GWCCG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGGWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     misc_feature    3526..3531
                     /Recognition_pattern="C^GTACG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGTACG"
                     /label="BsiWI, Pfl23II, PspLI"
                     /note="Geneious type: restriction site"
     misc_feature    3528..3533
                     /Recognition_pattern="TAC^GTA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    3533..3538
                     /Recognition_pattern="A^CTAGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    3548..3553
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    3576..4138
                     /note="/vntifkey=""""21"""""
                     /label="IRES"
     misc_feature    3894..3900
                     /Recognition_pattern="CACCTGC(4/8)"
                     /annotation_group="CACCTGC"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4105..4110)
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACGTC"
                     /label="BtrI, AjiI, BmgBI"
                     /note="Geneious type: restriction site"
     CDS             4142..4741
                     /note="/vntifkey=""""4"""""
                     /label="Puro(R)"
     misc_feature    4145..4754
                     /Original_Bases="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG
                     AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCA
                     GCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGG
                     GACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCT
                     GACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGG
                     TGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTG
                     ATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGA
                     GGGCTAACTAGCTAGTCGAGCTC"
                     /label="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGC
                     TACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCT
                     AGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTG
                     CAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTAT
                     CGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAG
                     GTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGC
                     CGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAACT
                     AGCTAGTCGAGCTC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4758..4763
                     /Recognition_pattern="TT^CGAA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TTCGAA"
                     /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV,
                     SfuI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4784..4800)
                     /note="/vntifkey=""""21"""""
                     /label="pBluescriptKS_primer"
     enhancer        4815..5407
                     /note="/vntifkey=""""9"""""
                     /label="WPRE"
     misc_feature    5473..5494
                     /note="/vntifkey=""""21"""""
                     /label="U3PPT"
     misc_feature    5473..5488
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    5545..5578
                     /note="/vntifkey=""""21"""""
                     /label="loxP"
     misc_feature    5598..5778
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    5598..5778
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    5782..5787
                     /Recognition_pattern="T^CTAGA"
                     /Methylation="Dam:blocked"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5804..5821)
                     /note="/vntifkey=""""21"""""
                     /label="Sp6_primer"
     misc_feature    5880..5885
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    6041..6046
                     /Recognition_pattern="G^CTAGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     misc_feature    6041..6046
                     /Recognition_pattern="GCTAG^C"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="BmtI, BspOI"
                     /note="Geneious type: restriction site"
     gene            6059..6322
                     /gene="tet (300 - 563)"
                     /note="/vntifkey=""""60"""""
     misc_feature    6082..6089
                     /Recognition_pattern="CR^CCGGYG"
                     /Methylation="CpG:variable, EcoBI:some blocked, EcoKI:some
                     blocked"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6111..6130)
                     /note="/vntifkey=""""21"""""
                     /label="pBRrevBam_primer"
     misc_feature    6167..6172
                     /Recognition_pattern="AGC^GCT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    6463..6485
                     /note="/vntifkey=""""21"""""
                     /label="pGEX_3_primer"
     promoter        6644..6672
                     /note="/vntifkey=""""30"""""
                     /label="AmpR_promoter"
     gene            6714..7574
                     /gene="Ampicillin"
                     /note="/vntifkey=""""60"""""
     CDS             6714..7574
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\3"
     misc_feature    7276..7281
                     /Recognition_pattern="TGC^GCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    7496..7506
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     rep_origin      7729..8348
                     /note="/vntifkey=""""33"""""
                     /label="pBR322_origin"
     misc_feature    complement(8574..8594)
                     /note="/vntifkey=""""21"""""
                     /label="pBABE_3_primer"
     misc_feature    complement(8580..9033)
                     /note="/vntifkey=""""21"""""
                     /label="SV40_enhancer"
     promoter        8592..8860
                     /note="/vntifkey=""""30"""""
                     /label="SV40_promoter"
     rep_origin      8759..8836
                     /note="/vntifkey=""""33"""""
                     /label="SV40_origin"
     misc_feature    8821..8840
                     /note="/vntifkey=""""21"""""
                     /label="SV40pro_F_primer"
     misc_feature    8839..8851
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /Methylation="Dcm:some impaired, CpG:variable"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(9389..9399)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /Methylation="Dcm:cut, CpG:variable, EcoBI:blocked"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    9719..9728
                     /Recognition_pattern="GACNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    9975..9987
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Methylation="CpG:-"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    10041..10056
                     /note="/vntifkey=""""21"""""
                     /label="SV40_int"
     misc_feature    10062..10109
                     /note="/vntifkey=""""21"""""
                     /label="SV40_3_splice"
     CDS             10186..10659
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\1"
     misc_feature    10237..10257
                     /note="/vntifkey=""""21"""""
                     /label="SV40\NLS"
     misc_feature    10243..10257
                     /note="/vntifkey=""""21"""""
                     /label="NLS"
     terminator      10685..10816
                     /note="/vntifkey=""""43"""""
                     /label="SV40_PA_terminator"
     misc_feature    10773..10792
                     /note="/vntifkey=""""21"""""
                     /label="EBV_rev_primer"
     misc_feature    10881..10895
                     /Recognition_pattern="CCANNNNN^NNNNTGG"
                     /Methylation="Dcm:cut"
                     /annotation_group="CCANNNNNNNNNTGG"
                     /label="XcmI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacccgggc gcgcctgcag gatccatatg cggaccgtac gtactagtgg
     3541 ccacggctga tcatacgggc tgcaggaatt ccgccccccc ccccctaacg ttactggccg
     3601 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3661 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3721 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3781 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3841 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3901 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3961 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4021 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4081 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatac
     4141 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4201 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4261 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4321 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4381 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4441 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4501 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4561 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4621 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4681 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4741 acgggcgcgt ctggaacttc gaattcgata tcaagcttat cgcgataccg tcgacctcga
     4801 gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4861 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4921 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4981 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5041 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5101 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5161 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     5221 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5281 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5341 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5401 catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     5461 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5521 agatgggatc aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc
     5581 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5761 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     5821 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     5881 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     5941 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     6001 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     6061 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     6121 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     6181 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     6241 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     6301 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     6361 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6421 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6481 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6541 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7861 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8101 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     8161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     8581 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8641 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8701 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8761 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8821 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8881 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     8941 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9001 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9061 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9121 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9181 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9241 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9301 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     9361 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     9421 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     9481 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     9541 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     9601 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     9661 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     9721 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     9781 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     9841 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     9901 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     9961 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10021 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10081 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10141 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10201 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10261 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10321 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10381 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    10441 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    10501 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    10561 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    10621 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    10681 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10741 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10801 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10861 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
    10921 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10981 tag
//
