LOCUS       pWPI-IRES-Puro-A       13400 bp ss-DNA     circular SYN 12-JUN-2020
DEFINITION  pWPI lentiviral system.
ACCESSION   -
KEYWORDS    -.
SOURCE      
  ORGANISM  .
COMMENT     #497=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
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            #501=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
            #502=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
            #503=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
            #504=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)
            #505=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
            #506=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
            #507=(CWidgetStyle "Void" 0 0 0 0 0) #508=(CWidgetStyle "General
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            "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) (LOGSHAPE 1 1.9 0 0 0))
            #491=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1
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            0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
            1.05 0 1 48 "@F bp (molecule @L bp)" 0) #496=(CWidgetStyle
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            site") 1 5 "TCTAGA" "AGATCT") #476=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92086176 (8 0) 1 1 1 0 178) (CObjectList))
            "XhoI: 3 sites") 1 5 "CTCGAG" "GAGCTC") #477=(CRSFolderPar
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            #468=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085472 (8
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            #469=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085408 (8
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            #483=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0
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            #484=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0
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            #481=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1
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            "RRE" "@N" 1 13.5098 5.22699 0 -10 1.98146 1.11561 #546#)
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            100) (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 1 5.08951
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            0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233
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            0.932452 Nil)) (CObjectList)) #526=(CGroupWidget (CWidget 10 (6 0)
            1 2 0 0 Nil -2538 100) (CObjectList #527=(CGroupWidget (CWidget 4
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            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
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            1.11561 #534#)) (CObjectList)) #536=(CGroupWidget (CWidget 9 (7 9
            0) 1 2 0 0 Nil -3061 100) (CObjectList #537=(CWideArrow (CWideLine
            (CWidget 0 (3 #114# 0) 1 2 0 0 #497# 99149004 100) (LOGPEN 0 0 0) 1
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            1 34 "Arial") 1.74233 0.666667 0 "ORF frame 3" "@N" 1 -16.0561
            -1.74233 0 -10 5.26169 1.11561 #530#) #532=(CWideArrow (CWideLine
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            3355545) 1 (LOGBRUSH 0 6724095 0) 1 0.188457 0.464163 1 0.082322)
            0.8 1.8 0) #533=(CLabel (CWidget 0 (0 0) 1 2 0 0 #482# 2136765432
            100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34
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            -10 5.26169 1.11561 #532#) #534=(CWideArrow (CObjectList
            #418=(CLinePar (CParagraph 0 (1 #64# 1) 1 2 2 0 191) " N1: 5844 "
            1))))) "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") #419=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92073792 (8 0) 1 1 1 0 178)
            (CObjectList)) "NlaIII: 32 sites") 5 1 "NCATGN" "NGTACN")
            #420=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073856 (8
            0) 1 1 1 0 178) (CObjectList #421=(CGroupPar (CParagraph 0 (10 #66#
            0) 1 2 2 0 180) (CObjectList #422=(CLinePar (CParagraph 0 (1 #66#
            1) 1 2 2 0 (CGroupPar (CParagraph 92064000 (8 0) 1 1 1 0 178)
            (CObjectList #413=(CGroupPar (CParagraph 0 (10 #62# 0) 1 2 2 0 180)
            (CObjectList #414=(CLinePar (CParagraph 0 (1 #62# 1) 1 2 2 0 191) "
            N1: 3517 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC")
            #415=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92060832 (8
            0) 1 1 1 0 178) (CObjectList)) "NgoMIV: 3 sites") 1 5 "GCCGGC"
            "CGGCCG") #416=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92064672 (8 0) 1 1 1 0 178) (CObjectList #417=(CGroupPar
            (CParagraph 0 (10 #64# 0) 1 2 2 0 180) (CParagraph 0 (1 #69# 1) 1 2
            2 0 191) " N1: 3322 " 1))))) "PacI: 1 site") 5 3 "TTAATTAA"
            "AATTAATT") #428=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92075680 (8 0) 1 1 1 0 178) (CObjectList)) "PciI: 4 sites") 1 5
            "ACATGT" "TGTACA") #429=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92076192 (8 0) 1 1 1 0 178) (CObjectList
            #430=(CGroupPar (CParagraph 0 (10 #71# 0) 1 2 2 0 180) (CObjectList
            #431=(CLinePar (CParagraph 0 (1 #71# 1) 1 2 2 0 191) " N1: 3491 "
            1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG")
            #432=(CRSFolderPar 191) " N1: 1146 " 1))))) "NotI: 1 site") 2 6
            "GCGGCCGC" "CGCCGGCG") #423=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92073920 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3
            sites") 3 3 "TCGCGA" "AGCGCT") #424=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92074816 (8 0) 1 1 1 0 178) (CObjectList))
            "NsiI: 2 sites") 5 1 "ATGCAT" "TACGTA") #425=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92078624 (8 0) 1 1 1 0 178)
            (CObjectList #426=(CGroupPar (CParagraph 0 (10 #69# 0) 1 2 2 0 180)
            (CObjectList #427=(CLinePar (CGroupPar (CParagraph 92549352 (8 0) 1
            1 1 0 178) (CObjectList)) "HpyCH4IV: 23 sites") 1 3 "ACGT" "TGCA")
            #401=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92549392 (8
            0) 1 1 1 0 178) (CObjectList)) "HpyCH4V: 51 sites") 2 2 "TGCA"
            "ACGT") #402=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92058368 (8 0) 1 1 1 0 178) (CObjectList)) "KasI: 5 sites") 1 5
            "GGCGCC" "CCGCGG") #403=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92056608 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 2
            sites") 5 1 "GGTACC" "CCATGG") #404=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058752 (8 0) 1 1 1 0 178) (CObjectList))
            "HinP1I: 45 sites") 1 3 "GCGC" "CGCG") #397=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92055008 (8 0) 1 1 1 0 178)
            (CObjectList)) "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG")
            #398=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055072 (8
            0) 1 1 1 0 178) (CObjectList)) "HpaII: 35 sites") 1 3 "CCGG"
            "GGCC") #399=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92055712 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4I: 32 sites") 5 1
            "NCATGN" "NGTACN") #400=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083872 (8 0) 1 1 1 0 178) (CObjectList)) "MseI: 55
            sites") 1 3 "TTAA" "AATT") #409=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058400 (8 0) 1 1 1 0 178) (CObjectList))
            "NaeI: 3 sites") 3 3 "GCCGGC" "CGGCCG") #410=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086784 (8 0) 1 1 1 0 178)
            (CObjectList)) "NarI: 5 sites") 2 4 "GGCGCC" "CCGCGG")
            #411=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92063808 (8
            0) 1 1 1 0 178) (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG"
            "GGTACC") #412=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92056800 (8 0) 1 1 1 0 178) (CObjectList)) "MboI: 50 sites") 1 5
            "NGATCN" "NCTAGN") #405=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92057888 (8 0) 1 1 1 0 178) (CObjectList)) "MfeI: 3
            sites") 1 5 "CAATTG" "GTTAAC") #406=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92057952 (8 0) 1 1 1 0 178) (CObjectList))
            "MluI: 0 sites") 1 5 "ACGCGT" "TGCGCA") #407=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92083904 (8 0) 1 1 1 0 178)
            (CObjectList)) "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT")
            #408=(CRSFolderPar (CFolderPar #454=(CGroupPar (CParagraph 0 (10
            #89# 0) 1 2 2 0 180) (CObjectList #455=(CLinePar (CParagraph 0 (1
            #89# 1) 1 2 2 0 191) " N1: 3531 " 1))))) "SnaBI: 1 site") 3 3
            "TACGTA" "ATGCAT") #456=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083936 (8 0) 1 1 1 0 178) (CObjectList
            #457=(CGroupPar (CParagraph 0 (10 #90# 0) 1 2 2 0 180) (CObjectList
            #458=(CLinePar (CParagraph 0 (1 #90# 1) 1 2 2 0 191) " N1: 3534 "
            1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #459=(CRSFolderPar
            #449=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084032 (8
            0) 1 1 1 0 178) (CObjectList)) "SfoI: 5 sites") 3 3 "GGCGCC"
            "CCGCGG") #450=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92084000 (8 0) 1 1 1 0 178) (CObjectList #451=(CGroupPar
            (CParagraph 0 (10 #88# 0) 1 2 2 0 180) (CObjectList #452=(CLinePar
            (CParagraph 0 (1 #88# 1) 1 2 2 0 191) " N1: 3497 " 1))))) "SmaI: 1
            site") 3 3 "CCCGGG" "GGGCCC") #453=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92083968 (8 0) 1 1 1 0 178) (CObjectList
            (CFolderPar (CGroupPar (CParagraph 92085920 (8 0) 1 1 1 0 178)
            (CObjectList)) "StuI: 3 sites") 3 3 "AGGCCT" "TCCGGA")
            #464=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085856 (8
            0) 1 1 1 0 178) (CObjectList #465=(CGroupPar (CParagraph 0 (10 #96#
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            700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "HIV-1 5 LTR" "@N"
            1 -4.37713 -19.1656 0 -10 4.97863 1.11561 #562#) #564=(CScratch
            (CWidget 0 (3 #120# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 3.01299 1.9 0.082322 1) #565=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 63286324 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "Sp6 primer" "@N" 1 -5.00421
            -15.681 0 -10 4.62896 1.11561 #564#) #566=(CScratch (CWidget 0 (3
            #121# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507) 10 1 2.83442
            1.9 0.082322 1) #567=(CLabel (CWideLine (CWidget 0 (3 #118# 0) 1 2
            0 0 #486# 99148984 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334
            0) 0.835356 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #561=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 1371 100) (LOGPEN 0 0 0) 1 (LOGFONT
            69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "truncHIV-1 3 LTR" "@N" 1 -5.56886 -20.908 0 -10 7.14325 1.11561
            #560#) #562=(CWideArrow (CWideLine (CWidget 0 (3 #119# 0) 1 2 0 0
            #486# 63308488 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0)
            0.670712 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #563=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (CObjectList
            #601=(CLine (CWidget 0 (3 #136# 0) 1 2 0 0 #490# 1834 100) (LOGPEN
            0 12 13395507) 10 0.835356 0.0971369 0.173916) #602=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 59796852 100) (LOGPEN 0 0 0) 1
            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "SV40 PA terminator" "@N" 1 -7.08378 20.908 0 -10 8.20891 1.11561
            #601#)) (CObjectList))) (CObjectList)) #603=(CGroupWidget (CWidget
            11 (8 0) 1 2 0 0 Nil -2119 100) (CObjectList #604=(CGroupWidget
            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1805 100) (CObjectList
            #605=(CScratch 0.082322) 0.8 1.8 0) #597=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "pBR322 origin" "@N" 1
            -16.4307 1.74233 0 -10 5.97768 1.11561 #596#) #598=(CScratch
            (CWidget 0 (3 #129# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 1.29419 1.9 0.082322 1) #599=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 59795284 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "SV40 origin" "@N" 1 -15.7111
            6.96932 0 -10 4.86207 1.11561 #598#)) (CObjectList))
            #600=(CGroupWidget (CWidget 43 (7 43 0) 1 2 0 0 Nil -2643 100)
            (LOGPEN 0 0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18
            "Georgia") 1.74233 0.555556 128 "{\\i Asc}I (3500)" "@N (@S)" 5
            15.2669 -15.681 0 -10 4.59566 0.932452 #608#)) (CObjectList))
            #610=(CGroupWidget.
FEATURES             Location/Qualifiers
     source          1..13400
                     /mol_type="other DNA"
                     /note="/vntifkey=""""98"""""
                     /organism="pWPI"
     misc_feature    309..314
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    712..717
                     /Recognition_pattern="G^CGCGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCGCGC"
                     /label="BsePI, BssHII, PauI, PteI"
                     /note="Geneious type: restriction site"
     misc_feature    746..790
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_psi_pack"
     misc_feature    1144..1151
                     /Recognition_pattern="GC^GGCCGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     CDS             1178..2035
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\2"
     misc_feature    1300..1533
                     /note="/vntifkey=""""21"""""
                     /label="RRE"
     misc_feature    1930..1936
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     promoter        2127..3297
                     /note="/vntifkey=""""30"""""
                     /label="EF1a_promoter"
     misc_feature    2818..2825
                     /Recognition_pattern="GGCCGG^CC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    2866..2872
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    3309..3316
                     /Recognition_pattern="ATTT^AAAT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATTTAAAT"
                     /label="SwaI, SmiI"
                     /note="Geneious type: restriction site"
     misc_feature    3317..3324
                     /Recognition_pattern="TTAAT^TAA"
                     /Methylation="EcoKI:cut"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     misc_feature    3349..3364
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    3487..3494
                     /Recognition_pattern="GTTT^AAAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     polylinker      3494..3499
                     /created_by="pbeare"
                     /modified_by="pbeare"
                     /label="SmaI site"
     misc_feature    3494..3499
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3494..3499
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     Kozak           3501..3508
                     /created_by="pbeare"
     CDS             3509..5926
                     /created_by="pbeare"
                     /label="ACE2"
     misc_feature    complement(5071..5084)
                     /Recognition_pattern="(7/12)GAANNNNNNNTTGG(11/6)"
                     /annotation_group="GAANNNNNNNTTGG"
                     /label="AjuI"
                     /note="Geneious type: restriction site"
     misc_feature    5926^5927
                     /Original_Bases="AGCAGGCACCATGGCTTTGAACTCAGGGTCACCACCAGCTA
                     TTGGACCTTACTATGAAAACCATGGATACCAACCGGAAAACCCCTATCCCGCACAGCC
                     CACTGTGGTCCCCACTGTCTACGAGGTGCATCCGGCTCAGTACTACCCGTCCCCCGTG
                     CCCCAGTACGCCCCGAGGGTCCTGACGCAGGCTTCCAACCCCGTCGTCTGCACGCAGC
                     CCAAATCCCCATCCGGGACAGTGTGCACCTCAAAGACTAAGAAAGCACTGTGCATCAC
                     CTTGACCCTGGGGACCTTCCTCGTGGGAGCTGCGCTGGCCGCTGGCCTACTCTGGAAG
                     TTCATGGGCAGCAAGTGCTCCAACTCTGGGATAGAGTGCGACTCCTCAGGTACCTGCA
                     TCAACCCCTCTAACTGGTGTGATGGCGTGTCACACTGCCCCGGCGGGGAGGACGAGAA
                     TCGGTGTGTTCGCCTCTACGGACCAAACTTCATCCTTCAGGTGTACTCATCTCAGAGG
                     AAGTCCTGGCACCCTGTGTGCCAAGACGACTGGAACGAGAACTACGGGCGGGCGGCCT
                     GCAGGGACATGGGCTATAAGAATAATTTTTACTCTAGCCAAGGAATAGTGGATGACAG
                     CGGATCCACCAGCTTTATGAAACTGAACACAAGTGCCGGCAATGTCGATATCTATAAA
                     AAACTGTACCACAGTGATGCCTGTTCTTCAAAAGCAGTGGTTTCTTTACGCTGTATAG
                     CCTGCGGGGTCAACTTGAACTCAAGCCGCCAGAGCAGGATCGTGGGCGGCGAGAGCGC
                     GCTCCCGGGGGCCTGGCCCTGGCAGGTCAGCCTGCACGTCCAGAACGTCCACGTGTGC
                     GGAGGCTCCATCATCACCCCCGAGTGGATCGTGACAGCCGCCCACTGCGTGGAAAAAC
                     CTCTTAACAATCCATGGCATTGGACGGCATTTGCGGGGATTTTGAGACAATCTTTCAT
                     GTTCTATGGAGCCGGATACCAAGTAGAAAAAGTGATTTCTCATCCAAATTATGACTCC
                     AAGACCAAGAACAATGACATTGCGCTGATGAAGCTGCAGAAGCCTCTGACTTTCAACG
                     ACCTAGTGAAACCAGTGTGTCTGCCCAACCCAGGCATGATGCTGCAGCCAGAACAGCT
                     CTGCTGGATTTCCGGGTGGGGGGCCACCGAGGAGAAAGGGAAGACCTCAGAAGTGCTG
                     AACGCTGCCAAGGTGCTTCTCATTGAGACACAGAGATGCAACAGCAGATATGTCTATG
                     ACAACCTGATCACACCAGCCATGATCTGTGCCGGCTTCCTGCAGGGGAACGTCGATTC
                     TTGCCAGGGTGACAGTGGAGGGCCTCTGGTCACTTCGAAGAACAATATCTGGTGGCTG
                     ATAGGGGATACAAGCTGGGGTTCTGGCTGTGCCAAAGCTTACAGACCAGGAGTGTACG
                     GGAATGTGATGGTATTCACGGACTGGATTTATCGACAAATGAGGGCAGACGGCTAG"
                     /label="AGCAGGCACCATGGCTTTGAACTCAGGGTCACCACCAGCTATTGGACCTT
                     ACTATGAAAACCATGGATACCAACCGGAAAACCCCTATCCCGCACAGCCCACTGTGGT
                     CCCCACTGTCTACGAGGTGCATCCGGCTCAGTACTACCCGTCCCCCGTGCCCCAGTAC
                     GCCCCGAGGGTCCTGACGCAGGCTTCCAACCCCGTCGTCTGCACGCAGCCCAAATCCC
                     CATCCGGGACAGTGTGCACCTCAAAGACTAAGAAAGCACTGTGCATCACCTTGACCCT
                     GGGGACCTTCCTCGTGGGAGCTGCGCTGGCCGCTGGCCTACTCTGGAAGTTCATGGGC
                     AGCAAGTGCTCCAACTCTGGGATAGAGTGCGACTCCTCAGGTACCTGCATCAACCCCT
                     CTAACTGGTGTGATGGCGTGTCACACTGCCCCGGCGGGGAGGACGAGAATCGGTGTGT
                     TCGCCTCTACGGACCAAACTTCATCCTTCAGGTGTACTCATCTCAGAGGAAGTCCTGG
                     CACCCTGTGTGCCAAGACGACTGGAACGAGAACTACGGGCGGGCGGCCTGCAGGGACA
                     TGGGCTATAAGAATAATTTTTACTCTAGCCAAGGAATAGTGGATGACAGCGGATCCAC
                     CAGCTTTATGAAACTGAACACAAGTGCCGGCAATGTCGATATCTATAAAAAACTGTAC
                     CACAGTGATGCCTGTTCTTCAAAAGCAGTGGTTTCTTTACGCTGTATAGCCTGCGGGG
                     TCAACTTGAACTCAAGCCGCCAGAGCAGGATCGTGGGCGGCGAGAGCGCGCTCCCGGG
                     GGCCTGGCCCTGGCAGGTCAGCCTGCACGTCCAGAACGTCCACGTGTGCGGAGGCTCC
                     ATCATCACCCCCGAGTGGATCGTGACAGCCGCCCACTGCGTGGAAAAACCTCTTAACA
                     ATCCATGGCATTGGACGGCATTTGCGGGGATTTTGAGACAATCTTTCATGTTCTATGG
                     AGCCGGATACCAAGTAGAAAAAGTGATTTCTCATCCAAATTATGACTCCAAGACCAAG
                     AACAATGACATTGCGCTGATGAAGCTGCAGAAGCCTCTGACTTTCAACGACCTAGTGA
                     AACCAGTGTGTCTGCCCAACCCAGGCATGATGCTGCAGCCAGAACAGCTCTGCTGGAT
                     TTCCGGGTGGGGGGCCACCGAGGAGAAAGGGAAGACCTCAGAAGTGCTGAACGCTGCC
                     AAGGTGCTTCTCATTGAGACACAGAGATGCAACAGCAGATATGTCTATGACAACCTGA
                     TCACACCAGCCATGATCTGTGCCGGCTTCCTGCAGGGGAACGTCGATTCTTGCCAGGG
                     TGACAGTGGAGGGCCTCTGGTCACTTCGAAGAACAATATCTGGTGGCTGATAGGGGAT
                     ACAAGCTGGGGTTCTGGCTGTGCCAAAGCTTACAGACCAGGAGTGTACGGGAATGTGA
                     TGGTATTCACGGACTGGATTTATCGACAAATGAGGGCAGACGGCTAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    5938..5944
                     /Recognition_pattern="CG^GWCCG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGGWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     misc_feature    5943..5948
                     /Recognition_pattern="C^GTACG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGTACG"
                     /label="BsiWI, Pfl23II, PspLI"
                     /note="Geneious type: restriction site"
     misc_feature    5945..5950
                     /Recognition_pattern="TAC^GTA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    5950..5955
                     /Recognition_pattern="A^CTAGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    5965..5970
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    5993..6555
                     /note="/vntifkey=""""21"""""
                     /label="IRES"
     misc_feature    6311..6317
                     /Recognition_pattern="CACCTGC(4/8)"
                     /annotation_group="CACCTGC"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6522..6527)
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACGTC"
                     /label="BtrI, AjiI, BmgBI"
                     /note="Geneious type: restriction site"
     CDS             6559..7158
                     /note="/vntifkey=""""4"""""
                     /label="Puro(R)"
     misc_feature    6562..7171
                     /Original_Bases="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG
                     AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCA
                     GCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGG
                     GACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCT
                     GACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGG
                     TGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTG
                     ATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGA
                     GGGCTAACTAGCTAGTCGAGCTC"
                     /label="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGC
                     TACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCT
                     AGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTG
                     CAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTAT
                     CGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAG
                     GTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGC
                     CGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAACT
                     AGCTAGTCGAGCTC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7175..7180
                     /Recognition_pattern="TT^CGAA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TTCGAA"
                     /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV,
                     SfuI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7201..7217)
                     /note="/vntifkey=""""21"""""
                     /label="pBluescriptKS_primer"
     enhancer        7232..7824
                     /note="/vntifkey=""""9"""""
                     /label="WPRE"
     misc_feature    7890..7911
                     /note="/vntifkey=""""21"""""
                     /label="U3PPT"
     misc_feature    7890..7905
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    7962..7995
                     /note="/vntifkey=""""21"""""
                     /label="loxP"
     misc_feature    8015..8195
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    8015..8195
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    8199..8204
                     /Recognition_pattern="T^CTAGA"
                     /Methylation="Dam:blocked"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8221..8238)
                     /note="/vntifkey=""""21"""""
                     /label="Sp6_primer"
     misc_feature    8297..8302
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    8458..8463
                     /Recognition_pattern="G^CTAGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     misc_feature    8458..8463
                     /Recognition_pattern="GCTAG^C"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="BmtI, BspOI"
                     /note="Geneious type: restriction site"
     gene            8476..8739
                     /gene="tet (300 - 563)"
                     /note="/vntifkey=""""60"""""
     misc_feature    8499..8506
                     /Recognition_pattern="CR^CCGGYG"
                     /Methylation="CpG:variable, EcoBI:some blocked, EcoKI:some
                     blocked"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8528..8547)
                     /note="/vntifkey=""""21"""""
                     /label="pBRrevBam_primer"
     misc_feature    8584..8589
                     /Recognition_pattern="AGC^GCT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    8880..8902
                     /note="/vntifkey=""""21"""""
                     /label="pGEX_3_primer"
     promoter        9061..9089
                     /note="/vntifkey=""""30"""""
                     /label="AmpR_promoter"
     gene            9131..9991
                     /gene="Ampicillin"
                     /note="/vntifkey=""""60"""""
     CDS             9131..9991
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\3"
     misc_feature    9693..9698
                     /Recognition_pattern="TGC^GCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     rep_origin      10146..10765
                     /note="/vntifkey=""""33"""""
                     /label="pBR322_origin"
     misc_feature    complement(10991..11011)
                     /note="/vntifkey=""""21"""""
                     /label="pBABE_3_primer"
     misc_feature    complement(10997..11450)
                     /note="/vntifkey=""""21"""""
                     /label="SV40_enhancer"
     promoter        11009..11277
                     /note="/vntifkey=""""30"""""
                     /label="SV40_promoter"
     rep_origin      11176..11253
                     /note="/vntifkey=""""33"""""
                     /label="SV40_origin"
     misc_feature    11238..11257
                     /note="/vntifkey=""""21"""""
                     /label="SV40pro_F_primer"
     misc_feature    11256..11268
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /Methylation="Dcm:some impaired, CpG:variable"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(11806..11816)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /Methylation="Dcm:cut, CpG:variable, EcoBI:blocked"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    12392..12404
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Methylation="CpG:-"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    12458..12473
                     /note="/vntifkey=""""21"""""
                     /label="SV40_int"
     misc_feature    12479..12526
                     /note="/vntifkey=""""21"""""
                     /label="SV40_3_splice"
     CDS             12603..13076
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\1"
     misc_feature    12654..12674
                     /note="/vntifkey=""""21"""""
                     /label="SV40\NLS"
     misc_feature    12660..12674
                     /note="/vntifkey=""""21"""""
                     /label="NLS"
     terminator      13102..13233
                     /note="/vntifkey=""""43"""""
                     /label="SV40_PA_terminator"
     misc_feature    13190..13209
                     /note="/vntifkey=""""21"""""
                     /label="EBV_rev_primer"
ORIGIN      
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacccggga gcgccaccat gtcaagctct tcctggctcc ttctcagcct
     3541 tgttgctgta actgctgctc agtccaccat tgaggaacag gccaagacat ttttggacaa
     3601 gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt ggaattataa
     3661 caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca aatggtctgc
     3721 ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa ttcagaatct
     3781 cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc tctcagaaga
     3841 caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca gtactggaaa
     3901 agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt tgaatgaaat
     3961 aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct ggagatctga
     4021 ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa atgagatggc
     4081 aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg aagtaaatgg
     4141 ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac atacctttga
     4201 agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt tgatgaatgc
     4261 ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg gtgatatgtg
     4321 gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga aaccaaacat
     4381 agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat tcaaggaggc
     4441 cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct gggaaaattc
     4501 catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag cttgggacct
     4561 ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg acttcctgac
     4621 agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac aaccttttct
     4681 gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca tgtcactttc
     4741 tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt ttcaagaaga
     4801 caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg ggactctgcc
     4861 atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa ttcccaaaga
     4921 ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggggtgg tggaacctgt
     4981 gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta atgattactc
     5041 attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag cactttgtca
     5101 agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta cagaagctgg
     5161 acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc tagcattgga
     5221 aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact ttgagccctt
     5281 atttacctgg ctgaaagacc agaacaagaa ttcttttgtg ggatggagta ccgactggag
     5341 tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc ttggagataa
     5401 agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg catatgctat
     5461 gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg aggatgtgcg
     5521 agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac ctaaaaatgt
     5581 gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc ggagccgtat
     5641 caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac agccaacact
     5701 tggacctcct aaccagcccc ctgtttccat atggctgatt gtttttggag ttgtgatggg
     5761 agtgatagtg gttggcattg tcatcctgat cttcactggg atcagagatc ggaagaagaa
     5821 aaataaagca agaagtggag aaaatcctta tgcctccatc gatattagca aaggagaaaa
     5881 taatccagga ttccaaaaca ctgatgatgt tcagacctcc ttttagggat ccatatgcgg
     5941 accgtacgta ctagtggcca cggctgatca tacgggctgc aggaattccg cccccccccc
     6001 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     6061 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     6121 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6181 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6241 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6301 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6361 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6421 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6481 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     6541 aaacacgatg ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6601 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6661 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6721 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6781 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6841 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6901 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6961 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7021 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7081 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7141 cgcaagcccg gtgcctgacg ggcgcgtctg gaacttcgaa ttcgatatca agcttatcgc
     7201 gataccgtcg acctcgaggg aattccgata atcaacctct ggattacaaa atttgtgaaa
     7261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7621 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7741 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7801 tttgggccgc ctccccgcat cgggaattcg agctcggtac ctttaagacc aatgacttac
     7861 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7921 cactcccaac gaagacaaga tgggatcaat tcaccatggg aataacttcg tatagcatac
     7981 attatacgaa gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg
     8041 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8101 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8161 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     8221 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     8281 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     8341 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     8401 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     8461 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     8521 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     8581 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     8641 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     8701 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     8761 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8821 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     8881 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     8941 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    10321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
    10561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10981 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    11041 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    11101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    11161 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    11221 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    11281 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
    11341 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
    11401 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
    11461 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
    11521 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
    11581 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
    11641 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
    11701 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
    11761 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
    11821 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
    11881 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
    11941 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
    12001 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
    12061 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
    12121 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
    12181 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    12241 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    12301 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    12361 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
    12421 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
    12481 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
    12541 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
    12601 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
    12661 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
    12721 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
    12781 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
    12841 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
    12901 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
    12961 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
    13021 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    13081 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    13141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    13201 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    13261 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
    13321 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    13381 atgtactaca aacttagtag
//
