LOCUS pWPI-IRES-Puro-A 12462 bp ss-DNA circular SYN 12-JUN-2020 DEFINITION pWPI lentiviral system. ACCESSION - KEYWORDS -. SOURCE ORGANISM . 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3 sites") 1 5 "CTCGAG" "GAGCTC") #477=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92086112 (8 0) 1 1 1 0 178) #468=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085472 (8 0) 1 1 1 0 178) (CObjectList)) "TaqI: 29 sites") 1 3 "TCGA" "AGCT") #469=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085408 (8 0) 1 1 1 0 178) (CObjectList)) "Tsp509I: 62 sites") 1 5 "NAATTN" "NTTAAN") #470=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92086304 (8 0) 1 1 1 0 178) (CObjectList #471=(CGroupPar (CParagraph 0 (10 #100# 0) 1 2 2 0 180) (CObjectList #472=(CLinePar (CParagraph 0 (1 #100# 1) 1 2 2 0 191) " N1: 3496 " 1))))) #482=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 "@N" 0) #483=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 "@L bp" 0) #484=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 "@N" 0) #485=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #486=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #487=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) #488=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 (LOGSHAPE 1 1.9 0 0 0)) #489=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 (LOGSHAPE 1 1.9 0 0 0)) #490=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 (CObjectList #478=(CGroupPar (CParagraph 0 (10 #103# 0) 1 2 2 0 180) (CObjectList #479=(CLinePar (CParagraph 0 (1 #103# 1) 1 2 2 0 191) " N1: 3495 " 1))))) "XmaI: 1 site") 1 5 "CCCGGG" "GGGCCC") #480=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92083744 (8 0) 1 1 1 0 178) (CObjectList)) "ZraI: 4 sites") 3 3 "GACGTC" "CTGCAG"))) "Restriction/Methylation Map: (no more than 1 site)")))) (CGraphView (CWStyleSheet (CObjectList 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#534=(CWideArrow (CObjectList #418=(CLinePar (CParagraph 0 (1 #64# 1) 1 2 2 0 191) " N1: 5844 " 1))))) "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") #419=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073792 (8 0) 1 1 1 0 178) (CObjectList)) "NlaIII: 32 sites") 5 1 "NCATGN" "NGTACN") #420=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073856 (8 0) 1 1 1 0 178) (CObjectList #421=(CGroupPar (CParagraph 0 (10 #66# 0) 1 2 2 0 180) (CObjectList #422=(CLinePar (CParagraph 0 (1 #66# 1) 1 2 2 0 (CGroupPar (CParagraph 92064000 (8 0) 1 1 1 0 178) (CObjectList #413=(CGroupPar (CParagraph 0 (10 #62# 0) 1 2 2 0 180) (CObjectList #414=(CLinePar (CParagraph 0 (1 #62# 1) 1 2 2 0 191) " N1: 3517 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC") #415=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92060832 (8 0) 1 1 1 0 178) (CObjectList)) "NgoMIV: 3 sites") 1 5 "GCCGGC" "CGGCCG") #416=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92064672 (8 0) 1 1 1 0 178) (CObjectList #417=(CGroupPar (CParagraph 0 (10 #64# 0) 1 2 2 0 180) (CParagraph 0 (1 #69# 1) 1 2 2 0 191) " N1: 3322 " 1))))) "PacI: 1 site") 5 3 "TTAATTAA" "AATTAATT") #428=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92075680 (8 0) 1 1 1 0 178) (CObjectList)) "PciI: 4 sites") 1 5 "ACATGT" "TGTACA") #429=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92076192 (8 0) 1 1 1 0 178) (CObjectList #430=(CGroupPar (CParagraph 0 (10 #71# 0) 1 2 2 0 180) (CObjectList #431=(CLinePar (CParagraph 0 (1 #71# 1) 1 2 2 0 191) " N1: 3491 " 1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG") #432=(CRSFolderPar 191) " N1: 1146 " 1))))) "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG") #423=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073920 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3 sites") 3 3 "TCGCGA" "AGCGCT") #424=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92074816 (8 0) 1 1 1 0 178) (CObjectList)) "NsiI: 2 sites") 5 1 "ATGCAT" "TACGTA") #425=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92078624 (8 0) 1 1 1 0 178) (CObjectList #426=(CGroupPar (CParagraph 0 (10 #69# 0) 1 2 2 0 180) (CObjectList #427=(CLinePar (CGroupPar (CParagraph 92549352 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4IV: 23 sites") 1 3 "ACGT" "TGCA") #401=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92549392 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4V: 51 sites") 2 2 "TGCA" "ACGT") #402=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92058368 (8 0) 1 1 1 0 178) (CObjectList)) "KasI: 5 sites") 1 5 "GGCGCC" "CCGCGG") #403=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92056608 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 2 sites") 5 1 "GGTACC" "CCATGG") #404=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92058752 (8 0) 1 1 1 0 178) (CObjectList)) "HinP1I: 45 sites") 1 3 "GCGC" "CGCG") #397=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055008 (8 0) 1 1 1 0 178) (CObjectList)) "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG") #398=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055072 (8 0) 1 1 1 0 178) (CObjectList)) "HpaII: 35 sites") 1 3 "CCGG" "GGCC") #399=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055712 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4I: 32 sites") 5 1 "NCATGN" "NGTACN") #400=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92083872 (8 0) 1 1 1 0 178) (CObjectList)) "MseI: 55 sites") 1 3 "TTAA" "AATT") #409=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92058400 (8 0) 1 1 1 0 178) (CObjectList)) "NaeI: 3 sites") 3 3 "GCCGGC" "CGGCCG") #410=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92086784 (8 0) 1 1 1 0 178) (CObjectList)) "NarI: 5 sites") 2 4 "GGCGCC" "CCGCGG") #411=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92063808 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG" "GGTACC") #412=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92056800 (8 0) 1 1 1 0 178) (CObjectList)) "MboI: 50 sites") 1 5 "NGATCN" "NCTAGN") #405=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92057888 (8 0) 1 1 1 0 178) (CObjectList)) "MfeI: 3 sites") 1 5 "CAATTG" "GTTAAC") #406=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92057952 (8 0) 1 1 1 0 178) (CObjectList)) "MluI: 0 sites") 1 5 "ACGCGT" "TGCGCA") #407=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92083904 (8 0) 1 1 1 0 178) (CObjectList)) "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT") #408=(CRSFolderPar (CFolderPar #454=(CGroupPar (CParagraph 0 (10 #89# 0) 1 2 2 0 180) (CObjectList #455=(CLinePar (CParagraph 0 (1 #89# 1) 1 2 2 0 191) " N1: 3531 " 1))))) "SnaBI: 1 site") 3 3 "TACGTA" "ATGCAT") #456=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92083936 (8 0) 1 1 1 0 178) (CObjectList #457=(CGroupPar (CParagraph 0 (10 #90# 0) 1 2 2 0 180) (CObjectList #458=(CLinePar (CParagraph 0 (1 #90# 1) 1 2 2 0 191) " N1: 3534 " 1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #459=(CRSFolderPar #449=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084032 (8 0) 1 1 1 0 178) (CObjectList)) "SfoI: 5 sites") 3 3 "GGCGCC" "CCGCGG") #450=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084000 (8 0) 1 1 1 0 178) (CObjectList #451=(CGroupPar 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(LOGPEN 0 0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233 0.555556 128 "{\\i Asc}I (3500)" "@N (@S)" 5 15.2669 -15.681 0 -10 4.59566 0.932452 #608#)) (CObjectList)) #610=(CGroupWidget. FEATURES Location/Qualifiers source 1..12462 /mol_type="other DNA" /note="/vntifkey=""""98""""" /organism="pWPI" misc_feature 309..314 /Recognition_pattern="T^CCGGA" /Methylation="Mixed Methylation" /annotation_group="TCCGGA" /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI" /note="Geneious type: restriction site" misc_feature 455..635 /note="/vntifkey=""""21""""" /label="truncHIV-1_3_LTR" misc_feature 455..635 /note="/vntifkey=""""21""""" /label="HIV-1_5_LTR" misc_feature 712..717 /Recognition_pattern="G^CGCGC" /Methylation="Mixed Methylation" /annotation_group="GCGCGC" /label="BsePI, BssHII, PauI, PteI" /note="Geneious type: restriction site" misc_feature 746..790 /note="/vntifkey=""""21""""" /label="HIV-1_psi_pack" misc_feature complement(1108..1114) /Recognition_pattern="GCTCTTC(1/4)" /Methylation="Mixed Methylation" /annotation_group="GCTCTTC" /label="SapI, BspQI, LguI, PciSI" /note="Geneious type: restriction site" misc_feature 1144..1151 /Recognition_pattern="GC^GGCCGC" /Methylation="Mixed Methylation" /annotation_group="GCGGCCGC" /label="NotI, CciNI" /note="Geneious type: restriction site" misc_feature 1145..1150 /Recognition_pattern="C^GGCCG" /Methylation="Mixed Methylation" /annotation_group="CGGCCG" /label="BseX3I, BstZI, EagI, EclXI, Eco52I" /note="Geneious type: restriction site" CDS 1178..2035 /note="/vntifkey=""""4""""" /label="ORF\frame\2" misc_feature 1300..1533 /note="/vntifkey=""""21""""" /label="RRE" misc_feature complement(1420..1426) /Recognition_pattern="CCTCAGC(-5/-2)" /Methylation="CpG:impaired, EcoBI:cut" /annotation_group="CCTCAGC" /label="BbvCI" /note="Geneious type: restriction site" misc_feature 1570..1579 /Recognition_pattern="CACNN^NNGTG" /Methylation="Mixed Methylation" /annotation_group="CACNNNNGTG" /label="OliI, AleI" /note="Geneious type: restriction site" misc_feature 1930..1936 /Recognition_pattern="GG^GWCCC" /annotation_group="GGGWCCC" /label="KflI" /note="Geneious type: restriction site" misc_feature 2019..2024 /Recognition_pattern="AT^CGAT" /Methylation="Mixed Methylation" /annotation_group="ATCGAT" /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI" /note="Geneious type: restriction site" misc_feature 2025..2030 /Recognition_pattern="G^TCGAC" /Methylation="CpG:some blocked" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" promoter 2127..3297 /note="/vntifkey=""""30""""" /label="EF1a_promoter" misc_feature 2198..2203 /Recognition_pattern="A^CCGGT" /Methylation="Mixed Methylation" /annotation_group="ACCGGT" /label="AgeI, AsiGI, BshTI, CspAI, PinAI" /note="Geneious type: restriction site" misc_feature 2818..2825 /Recognition_pattern="GGCCGG^CC" /Methylation="Mixed Methylation" /annotation_group="GGCCGGCC" /label="FseI, RigI" /note="Geneious type: restriction site" misc_feature 2866..2872 /Recognition_pattern="CC^CWGGG" /annotation_group="CCCWGGG" /label="PasI" /note="Geneious type: restriction site" misc_feature 3020..3026 /Recognition_pattern="CCTCAGC(-5/-2)" /Methylation="CpG:impaired, EcoBI:cut" /annotation_group="CCTCAGC" /label="BbvCI" /note="Geneious type: restriction site" misc_feature 3309..3316 /Recognition_pattern="ATTT^AAAT" /Methylation="Mixed Methylation" /annotation_group="ATTTAAAT" /label="SwaI, SmiI" /note="Geneious type: restriction site" misc_feature 3317..3324 /Recognition_pattern="TTAAT^TAA" /Methylation="EcoKI:cut" /annotation_group="TTAATTAA" /label="PacI" /note="Geneious type: restriction site" misc_feature 3339..3344 /Recognition_pattern="GTT^AAC" /Methylation="Mixed Methylation" /annotation_group="GTTAAC" /label="HpaI, KspAI" /note="Geneious type: restriction site" misc_feature 3349..3364 /note="/vntifkey=""""21""""" /label="cPPT" misc_feature 3463..3468 /Recognition_pattern="AT^CGAT" /Methylation="Mixed Methylation" /annotation_group="ATCGAT" /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI" /note="Geneious type: restriction site" misc_feature 3480..3487 /Recognition_pattern="VC^TCGAGB" /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut" /annotation_group="VCTCGAGB" /label="PspXI" /note="Geneious type: restriction site" misc_feature 3480..3487 /Recognition_pattern="CC^TCGAGG" /Methylation="CpG:-" /annotation_group="CCTCGAGG" /label="AbsI" /note="Geneious type: restriction site" misc_feature 3487..3494 /Recognition_pattern="GTTT^AAAC" /Methylation="Mixed Methylation" /annotation_group="GTTTAAAC" /label="PmeI, MssI" /note="Geneious type: restriction site" polylinker 3494..3499 /created_by="pbeare" /modified_by="pbeare" /label="SmaI/XmaI site" misc_feature 3494..3499 /Recognition_pattern="C^CCGGG" /Methylation="Mixed Methylation" /annotation_group="CCCGGG" /label="Cfr9I, TspMI, XmaI" /note="Geneious type: restriction site" misc_feature 3494..3499 /Recognition_pattern="CCC^GGG" /Methylation="CpG:blocked" /annotation_group="CCCGGG" /label="SmaI" /note="Geneious type: restriction site" source <3500..>3509 /organism="synthetic DNA construct" /mol_type="other DNA" Kozak 3501..3509 /created_by="pbeare" /label="Kozak sequence" CDS 3510..4988 /created_by="pbeare" /modified_by="pbeare" /label="TMPRSS2" misc_feature 3526..3532 /Recognition_pattern="G^GTNACC" /Methylation="Mixed Methylation" /annotation_group="GGTNACC" /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI" /note="Geneious type: restriction site" misc_feature 3778..3784 /Recognition_pattern="CC^CWGGG" /annotation_group="CCCWGGG" /label="PasI" /note="Geneious type: restriction site" misc_feature 3874..3880 /Recognition_pattern="CC^TNAGG" /Methylation="Mixed Methylation" /annotation_group="CCTNAGG" /label="AxyI, Bse21I, Bsu36I, Eco81I" /note="Geneious type: restriction site" misc_feature 4037..4049 /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)" /Methylation="CpG:impaired" /annotation_group="GAACNNNNNNTAC" /label="PsrI" /note="Geneious type: restriction site" misc_feature 4060..4067 /Recognition_pattern="CCTGCA^GG" /annotation_group="CCTGCAGG" /label="Sse8387I, SbfI, SdaI" /note="Geneious type: restriction site" misc_feature 4122..4127 /Recognition_pattern="G^GATCC" /Methylation="Dam:cut, Dcm:cut, CpG:cut" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 4207..4218 /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)" /Methylation="CpG:cut" /annotation_group="CAANNNNNGTGG" /label="CspCI" /note="Geneious type: restriction site" misc_feature 4291..4296 /Recognition_pattern="G^CGCGC" /Methylation="Mixed Methylation" /annotation_group="GCGCGC" /label="BsePI, BssHII, PauI, PteI" /note="Geneious type: restriction site" misc_feature 4298..4303 /Recognition_pattern="C^CCGGG" /Methylation="Mixed Methylation" /annotation_group="CCCGGG" /label="Cfr9I, TspMI, XmaI" /note="Geneious type: restriction site" misc_feature 4298..4303 /Recognition_pattern="CCC^GGG" /Methylation="CpG:blocked" /annotation_group="CCCGGG" /label="SmaI" /note="Geneious type: restriction site" misc_feature 4329..4334 /Recognition_pattern="CACGTC(-3/-3)" /Methylation="Mixed Methylation" /annotation_group="CACGTC" /label="BtrI, AjiI, BmgBI" /note="Geneious type: restriction site" misc_feature 4422..4433 /Recognition_pattern="CCANNNNN^NTGG" /Methylation="Dcm:variable" /annotation_group="CCANNNNNNTGG" /label="BstXI" /note="Geneious type: restriction site" misc_feature 4765..4770 /Recognition_pattern="T^GATCA" /Methylation="Mixed Methylation" /annotation_group="TGATCA" /label="BclI, FbaI, Ksp22I" /note="Geneious type: restriction site" misc_feature 4796..4803 /Recognition_pattern="CCTGCA^GG" /annotation_group="CCTGCAGG" /label="Sse8387I, SbfI, SdaI" /note="Geneious type: restriction site" misc_feature 4820..4834 /Recognition_pattern="CCANNNNN^NNNNTGG" /Methylation="Dcm:cut" /annotation_group="CCANNNNNNNNNTGG" /label="XcmI" /note="Geneious type: restriction site" misc_feature 4850..4855 /Recognition_pattern="TT^CGAA" /Methylation="Mixed Methylation" /annotation_group="TTCGAA" /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV, SfuI" /note="Geneious type: restriction site" misc_feature 4989..4994 /Recognition_pattern="G^GATCC" /Methylation="Dam:cut, Dcm:cut, CpG:cut" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 4994..4999 /Recognition_pattern="CA^TATG" /Methylation="Mixed Methylation" /annotation_group="CATATG" /label="NdeI, FauNDI" /note="Geneious type: restriction site" misc_feature 5000..5006 /Recognition_pattern="CG^GWCCG" /Methylation="Mixed Methylation" /annotation_group="CGGWCCG" /label="RsrII, CpoI, CspI, Rsr2I" /note="Geneious type: restriction site" misc_feature 5005..5010 /Recognition_pattern="C^GTACG" /Methylation="Mixed Methylation" /annotation_group="CGTACG" /label="BsiWI, Pfl23II, PspLI" /note="Geneious type: restriction site" misc_feature 5007..5012 /Recognition_pattern="TAC^GTA" /Methylation="Mixed Methylation" /annotation_group="TACGTA" /label="SnaBI, BstSNI, Eco105I" /note="Geneious type: restriction site" misc_feature 5012..5017 /Recognition_pattern="A^CTAGT" /Methylation="Mixed Methylation" /annotation_group="ACTAGT" /label="SpeI, AhlI, BcuI" /note="Geneious type: restriction site" misc_feature 5017..5022 /Recognition_pattern="TGG^CCA" /Methylation="Mixed Methylation" /annotation_group="TGGCCA" /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I" /note="Geneious type: restriction site" misc_feature 5027..5032 /Recognition_pattern="T^GATCA" /Methylation="Mixed Methylation" /annotation_group="TGATCA" /label="BclI, FbaI, Ksp22I" /note="Geneious type: restriction site" misc_feature 5055..5617 /note="/vntifkey=""""21""""" /label="IRES" misc_feature 5195..5200 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII, AspA2I, BlnI, XmaJI" /note="Geneious type: restriction site" misc_feature 5373..5379 /Recognition_pattern="CACCTGC(4/8)" /annotation_group="CACCTGC" /label="AarI" /note="Geneious type: restriction site" misc_feature complement(5584..5589) /Recognition_pattern="CACGTC(-3/-3)" /Methylation="Mixed Methylation" /annotation_group="CACGTC" /label="BtrI, AjiI, BmgBI" /note="Geneious type: restriction site" CDS 5621..6220 /note="/vntifkey=""""4""""" /label="Puro(R)" misc_feature 5624..6233 /Original_Bases="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCA GCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGG GACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCT GACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGG TGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTG ATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGA GGGCTAACTAGCTAGTCGAGCTC" /label="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGC TACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCT AGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTG CAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTAT CGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAG GTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGC CGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAACT AGCTAGTCGAGCTC" /note="Geneious type: Editing History Replacement" misc_feature 6237..6242 /Recognition_pattern="TT^CGAA" /Methylation="Mixed Methylation" /annotation_group="TTCGAA" /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV, SfuI" /note="Geneious type: restriction site" misc_feature complement(6263..6279) /note="/vntifkey=""""21""""" /label="pBluescriptKS_primer" misc_feature 6269..6274 /Recognition_pattern="G^TCGAC" /Methylation="CpG:some blocked" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 6274..6281 /Recognition_pattern="VC^TCGAGB" /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut" /annotation_group="VCTCGAGB" /label="PspXI" /note="Geneious type: restriction site" misc_feature 6274..6281 /Recognition_pattern="CC^TCGAGG" /Methylation="CpG:-" /annotation_group="CCTCGAGG" /label="AbsI" /note="Geneious type: restriction site" enhancer 6294..6886 /note="/vntifkey=""""9""""" /label="WPRE" misc_feature 6952..6973 /note="/vntifkey=""""21""""" /label="U3PPT" misc_feature 6952..6967 /note="/vntifkey=""""21""""" /label="cPPT" misc_feature 7024..7057 /note="/vntifkey=""""21""""" /label="loxP" misc_feature 7077..7257 /note="/vntifkey=""""21""""" /label="truncHIV-1_3_LTR" misc_feature 7077..7257 /note="/vntifkey=""""21""""" /label="HIV-1_5_LTR" misc_feature 7261..7266 /Recognition_pattern="T^CTAGA" /Methylation="Dam:blocked" /annotation_group="TCTAGA" /label="XbaI" /note="Geneious type: restriction site" misc_feature complement(7283..7300) /note="/vntifkey=""""21""""" /label="Sp6_primer" misc_feature 7359..7364 /Recognition_pattern="T^GTACA" /annotation_group="TGTACA" /label="Bsp1407I, BsrGI, BstAUI" /note="Geneious type: restriction site" misc_feature complement(7509..7520) /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)" /Methylation="Dam:some blocked, CpG:some blocked" /annotation_group="CGANNNNNNTGC" /label="BcgI" /note="Geneious type: restriction site" misc_feature 7520..7525 /Recognition_pattern="G^CTAGC" /Methylation="Mixed Methylation" /annotation_group="GCTAGC" /label="NheI, AsuNHI" /note="Geneious type: restriction site" misc_feature 7520..7525 /Recognition_pattern="GCTAG^C" /Methylation="Mixed Methylation" /annotation_group="GCTAGC" /label="BmtI, BspOI" /note="Geneious type: restriction site" gene 7538..7801 /gene="tet (300 - 563)" /note="/vntifkey=""""60""""" misc_feature 7561..7568 /Recognition_pattern="CR^CCGGYG" /Methylation="CpG:variable, EcoBI:some blocked, EcoKI:some blocked" /annotation_group="CRCCGGYG" /label="SgrAI" /note="Geneious type: restriction site" misc_feature complement(7590..7609) /note="/vntifkey=""""21""""" /label="pBRrevBam_primer" misc_feature 7646..7651 /Recognition_pattern="AGC^GCT" /Methylation="Mixed Methylation" /annotation_group="AGCGCT" /label="Eco47III, AfeI, Aor51HI" /note="Geneious type: restriction site" misc_feature 7937..7942 /Recognition_pattern="CGTCTC(1/5)" /Methylation="Mixed Methylation" /annotation_group="CGTCTC" /label="Esp3I, BsmBI" /note="Geneious type: restriction site" misc_feature 7942..7964 /note="/vntifkey=""""21""""" /label="pGEX_3_primer" misc_feature complement(7991..7996) /Recognition_pattern="CGTCTC(1/5)" /Methylation="Mixed Methylation" /annotation_group="CGTCTC" /label="Esp3I, BsmBI" /note="Geneious type: restriction site" promoter 8123..8151 /note="/vntifkey=""""30""""" /label="AmpR_promoter" gene 8193..9053 /gene="Ampicillin" /note="/vntifkey=""""60""""" CDS 8193..9053 /note="/vntifkey=""""4""""" /label="ORF\frame\3" misc_feature complement(8453..8464) /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)" /Methylation="Dam:some blocked, CpG:some blocked" /annotation_group="CGANNNNNNTGC" /label="BcgI" /note="Geneious type: restriction site" misc_feature 8608..8613 /Recognition_pattern="CGAT^CG" /Methylation="Mixed Methylation" /annotation_group="CGATCG" /label="PvuI, Ple19I" /note="Geneious type: restriction site" misc_feature 8755..8760 /Recognition_pattern="TGC^GCA" /Methylation="Mixed Methylation" /annotation_group="TGCGCA" /label="Acc16I, FspI, NsbI" /note="Geneious type: restriction site" misc_feature 8975..8985 /Recognition_pattern="GACNNN^NNGTC" /Methylation="Mixed Methylation" /annotation_group="GACNNNNNGTC" /label="Eam1105I, AhdI, BmeRI, DriI" /note="Geneious type: restriction site" rep_origin 9208..9827 /note="/vntifkey=""""33""""" /label="pBR322_origin" misc_feature complement(9990..9996) /Recognition_pattern="GCTCTTC(1/4)" /Methylation="Mixed Methylation" /annotation_group="GCTCTTC" /label="SapI, BspQI, LguI, PciSI" /note="Geneious type: restriction site" misc_feature complement(10053..10073) /note="/vntifkey=""""21""""" /label="pBABE_3_primer" misc_feature complement(10059..10512) /note="/vntifkey=""""21""""" /label="SV40_enhancer" promoter 10071..10339 /note="/vntifkey=""""30""""" /label="SV40_promoter" misc_feature 10118..10123 /Recognition_pattern="ATGCA^T" /Methylation="Mixed Methylation" /annotation_group="ATGCAT" /label="EcoT22I, Mph1103I, NsiI, Zsp2I" /note="Geneious type: restriction site" misc_feature 10139..10145 /Recognition_pattern="A^CCWGGT" /Methylation="Mixed Methylation" /annotation_group="ACCWGGT" /label="SexAI, CsiI, MabI" /note="Geneious type: restriction site" misc_feature 10190..10195 /Recognition_pattern="ATGCA^T" /Methylation="Mixed Methylation" /annotation_group="ATGCAT" /label="EcoT22I, Mph1103I, NsiI, Zsp2I" /note="Geneious type: restriction site" rep_origin 10238..10315 /note="/vntifkey=""""33""""" /label="SV40_origin" misc_feature 10300..10319 /note="/vntifkey=""""21""""" /label="SV40pro_F_primer" misc_feature 10318..10330 /Recognition_pattern="GGCCNNNN^NGGCC" /Methylation="Dcm:some impaired, CpG:variable" /annotation_group="GGCCNNNNNGGCC" /label="SfiI" /note="Geneious type: restriction site" misc_feature 10372..10377 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII, AspA2I, BlnI, XmaJI" /note="Geneious type: restriction site" misc_feature 10851..10857 /Recognition_pattern="A^CCWGGT" /Methylation="Mixed Methylation" /annotation_group="ACCWGGT" /label="SexAI, CsiI, MabI" /note="Geneious type: restriction site" misc_feature 10862..10867 /Recognition_pattern="A^CCGGT" /Methylation="Mixed Methylation" /annotation_group="ACCGGT" /label="AgeI, AsiGI, BshTI, CspAI, PinAI" /note="Geneious type: restriction site" misc_feature complement(10868..10878) /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)" /Methylation="Dcm:cut, CpG:variable, EcoBI:blocked" /annotation_group="ACNNNNGTAYC" /label="BaeI" /note="Geneious type: restriction site" misc_feature 11198..11207 /Recognition_pattern="GACNN^NNGTC" /Methylation="Mixed Methylation" /annotation_group="GACNNNNGTC" /label="PshAI, BoxI, BstPAI" /note="Geneious type: restriction site" misc_feature 11454..11466 /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)" /Methylation="CpG:-" /annotation_group="GAAGNNNNNNTAC" /label="BarI" /note="Geneious type: restriction site" misc_feature 11520..11535 /note="/vntifkey=""""21""""" /label="SV40_int" misc_feature 11541..11588 /note="/vntifkey=""""21""""" /label="SV40_3_splice" CDS 11665..12138 /note="/vntifkey=""""4""""" /label="ORF\frame\1" misc_feature 11716..11736 /note="/vntifkey=""""21""""" /label="SV40\NLS" misc_feature 11722..11736 /note="/vntifkey=""""21""""" /label="NLS" misc_feature 12158..12163 /Recognition_pattern="GTT^AAC" /Methylation="Mixed Methylation" /annotation_group="GTTAAC" /label="HpaI, KspAI" /note="Geneious type: restriction site" terminator 12164..12295 /note="/vntifkey=""""43""""" /label="SV40_PA_terminator" misc_feature 12252..12271 /note="/vntifkey=""""21""""" /label="EBV_rev_primer" misc_feature 12360..12374 /Recognition_pattern="CCANNNNN^NNNNTGG" /Methylation="Dcm:cut" /annotation_group="CCANNNNNNNNNTGG" /label="XcmI" /note="Geneious type: restriction site" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt 2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt 2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg 2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg 2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt 2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga 3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg 3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc 3481 ctcgaggttt aaacccggga gcaggcacca tggctttgaa ctcagggtca ccaccagcta 3541 ttggacctta ctatgaaaac catggatacc aaccggaaaa cccctatccc gcacagccca 3601 ctgtggtccc cactgtctac gaggtgcatc cggctcagta ctacccgtcc cccgtgcccc 3661 agtacgcccc gagggtcctg acgcaggctt ccaaccccgt cgtctgcacg cagcccaaat 3721 ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc actgtgcatc accttgaccc 3781 tggggacctt cctcgtggga gctgcgctgg ccgctggcct actctggaag ttcatgggca 3841 gcaagtgctc caactctggg atagagtgcg actcctcagg tacctgcatc aacccctcta 3901 actggtgtga tggcgtgtca cactgccccg gcggggagga cgagaatcgg tgtgttcgcc 3961 tctacggacc aaacttcatc cttcaggtgt actcatctca gaggaagtcc tggcaccctg 4021 tgtgccaaga cgactggaac gagaactacg ggcgggcggc ctgcagggac atgggctata 4081 agaataattt ttactctagc caaggaatag tggatgacag cggatccacc agctttatga 4141 aactgaacac aagtgccggc aatgtcgata tctataaaaa actgtaccac agtgatgcct 4201 gttcttcaaa agcagtggtt tctttacgct gtatagcctg cggggtcaac ttgaactcaa 4261 gccgccagag caggatcgtg ggcggcgaga gcgcgctccc gggggcctgg ccctggcagg 4321 tcagcctgca cgtccagaac gtccacgtgt gcggaggctc catcatcacc cccgagtgga 4381 tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa tccatggcat tggacggcat 4441 ttgcggggat tttgagacaa tctttcatgt tctatggagc cggataccaa gtagaaaaag 4501 tgatttctca tccaaattat gactccaaga ccaagaacaa tgacattgcg ctgatgaagc 4561 tgcagaagcc tctgactttc aacgacctag tgaaaccagt gtgtctgccc aacccaggca 4621 tgatgctgca gccagaacag ctctgctgga tttccgggtg gggggccacc gaggagaaag 4681 ggaagacctc agaagtgctg aacgctgcca aggtgcttct cattgagaca cagagatgca 4741 acagcagata tgtctatgac aacctgatca caccagccat gatctgtgcc ggcttcctgc 4801 aggggaacgt cgattcttgc cagggtgaca gtggagggcc tctggtcact tcgaagaaca 4861 atatctggtg gctgataggg gatacaagct ggggttctgg ctgtgccaaa gcttacagac 4921 caggagtgta cgggaatgtg atggtattca cggactggat ttatcgacaa atgagggcag 4981 acggctaggg atccatatgc ggaccgtacg tactagtggc cacggctgat catacgggct 5041 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg 5101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 5161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 5221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 5281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 5341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 5401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 5461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 5521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac 5581 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atgaccgagt acaagcccac 5641 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 5701 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 5761 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 5821 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 5881 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 5941 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 6001 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 6061 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 6121 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 6181 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgggcgcgtc tggaacttcg 6241 aattcgatat caagcttatc gcgataccgt cgacctcgag ggaattccga taatcaacct 6301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6661 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 6721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 6841 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cgagctcggt 6901 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6961 ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg 7021 ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt 7081 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 7141 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7201 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca 7261 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa 7321 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc 7381 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt 7441 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga 7501 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat 7561 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 7621 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 7681 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 7741 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 7801 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 7861 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 7921 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 7981 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 8041 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 8101 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 8161 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 8221 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 8281 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 8341 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 8401 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 8461 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 8521 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 8581 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 8641 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 8701 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 8761 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 8821 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 8881 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 8941 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 9001 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 9061 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 9121 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 9181 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 9241 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 9301 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 9361 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 9421 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 9481 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 9541 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 9601 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 9661 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 9721 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 9781 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 9841 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 9901 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 9961 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 10021 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa 10081 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 10141 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 10201 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 10261 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 10321 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 10381 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc 10441 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta 10501 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg 10561 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt 10621 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca 10681 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat 10741 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa 10801 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga 10861 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat 10921 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga 10981 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg 11041 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac 11101 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa 11161 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc 11221 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc 11281 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg 11341 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg 11401 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa 11461 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag 11521 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta 11581 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag 11641 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct 11701 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca 11761 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct 11821 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct 11881 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca 11941 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt 12001 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat 12061 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 12121 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 12181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 12241 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 12301 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 12361 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 12421 aaagaaattg tatttgttaa atatgtacta caaacttagt ag //