LOCUS       pWPI-IRES-Puro-A       14904 bp ss-DNA     circular SYN 12-JUN-2020
DEFINITION  pWPI lentiviral system.
ACCESSION   -
KEYWORDS    -.
SOURCE      
  ORGANISM  .
COMMENT     #497=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
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            #501=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
            #502=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
            #503=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
            #504=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)
            #505=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
            #506=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
            #507=(CWidgetStyle "Void" 0 0 0 0 0) #508=(CWidgetStyle "General
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            "Times New Roman") 0.91 0 1 3 "@T @N " 0) #509=(CWidgetStyle
            "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) (LOGSHAPE 1 1.9 0 0 0))
            #491=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1
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            0) #494=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1
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            65535 "@N (@H)" 0) #495=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN
            0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
            1.05 0 1 48 "@F bp (molecule @L bp)" 0) #496=(CWidgetStyle
            "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0
            0 0 3 2 1 34 "Arial") 0.91 0 1 1 "Fragment of @N" 0) (LOGSHAPE 9 1
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            0.164644 1.74233 0.164644 2.53336 (70 (CShapeMapEntry 0 "Unique
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            site") 1 5 "TCTAGA" "AGATCT") #476=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92086176 (8 0) 1 1 1 0 178) (CObjectList))
            "XhoI: 3 sites") 1 5 "CTCGAG" "GAGCTC") #477=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086112 (8 0) 1 1 1 0 178)
            #468=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085472 (8
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            #469=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085408 (8
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            "NTTAAN") #470=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
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            #483=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0
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            #484=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 400 0
            0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 "@N" 0) #485=(CWidgetStyle
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            #481=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1
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            "RRE" "@N" 1 13.5098 5.22699 0 -10 1.98146 1.11561 #546#)
            #528=(CWideArrow (CWideLine (CWidget 0 (3 #108# 0) 1 2 0 0 #485# 0
            100) (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 1 5.08951
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            0 #482# 268889064 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0
            0 0 3 2 1 34 "Arial") 1.74233 0.666667 0 "ORF frame 2" "@N" 1
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            0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 1.74233
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            0.932452 Nil)) (CObjectList)) #526=(CGroupWidget (CWidget 10 (6 0)
            1 2 0 0 Nil -2538 100) (CObjectList #527=(CGroupWidget (CWidget 4
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            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "blasticidin resistance" "@N" 1 13.4697 -31.3619 0 -10 8.79169
            1.11561 #534#)) (CObjectList)) #536=(CGroupWidget (CWidget 9 (7 9
            0) 1 2 0 0 Nil -3061 100) (CObjectList #537=(CWideArrow (CWideLine
            (CWidget 0 (3 #114# 0) 1 2 0 0 #497# 99149004 100) (LOGPEN 0 0 0) 1
            (LOGBRUSH 2 8388608 5) 1 3.24333 3.58825 1 (CWidget 0 (0 0) 1 2 0 0
            #482# 2188 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2
            1 34 "Arial") 1.74233 0.666667 0 "ORF frame 3" "@N" 1 -16.0561
            -1.74233 0 -10 5.26169 1.11561 #530#) #532=(CWideArrow (CWideLine
            (CWidget 0 (3 #133# 0) 1 2 0 0 #485# 62653014 100) (LOGPEN 0 0
            3355545) 1 (LOGBRUSH 0 6724095 0) 1 0.188457 0.464163 1 0.082322)
            0.8 1.8 0) #533=(CLabel (CWidget 0 (0 0) 1 2 0 0 #482# 2136765432
            100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34
            "Arial") 1.74233 0.666667 0 "ORF frame 1" "@N" 1 -8.04783 19.1656 0
            -10 5.26169 1.11561 #532#) #534=(CWideArrow (CObjectList
            #418=(CLinePar (CParagraph 0 (1 #64# 1) 1 2 2 0 191) " N1: 5844 "
            1))))) "NheI: 1 site") 1 5 "GCTAGC" "CGATCG") #419=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92073792 (8 0) 1 1 1 0 178)
            (CObjectList)) "NlaIII: 32 sites") 5 1 "NCATGN" "NGTACN")
            #420=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92073856 (8
            0) 1 1 1 0 178) (CObjectList #421=(CGroupPar (CParagraph 0 (10 #66#
            0) 1 2 2 0 180) (CObjectList #422=(CLinePar (CParagraph 0 (1 #66#
            1) 1 2 2 0 (CGroupPar (CParagraph 92064000 (8 0) 1 1 1 0 178)
            (CObjectList #413=(CGroupPar (CParagraph 0 (10 #62# 0) 1 2 2 0 180)
            (CObjectList #414=(CLinePar (CParagraph 0 (1 #62# 1) 1 2 2 0 191) "
            N1: 3517 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC")
            #415=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92060832 (8
            0) 1 1 1 0 178) (CObjectList)) "NgoMIV: 3 sites") 1 5 "GCCGGC"
            "CGGCCG") #416=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92064672 (8 0) 1 1 1 0 178) (CObjectList #417=(CGroupPar
            (CParagraph 0 (10 #64# 0) 1 2 2 0 180) (CParagraph 0 (1 #69# 1) 1 2
            2 0 191) " N1: 3322 " 1))))) "PacI: 1 site") 5 3 "TTAATTAA"
            "AATTAATT") #428=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92075680 (8 0) 1 1 1 0 178) (CObjectList)) "PciI: 4 sites") 1 5
            "ACATGT" "TGTACA") #429=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92076192 (8 0) 1 1 1 0 178) (CObjectList
            #430=(CGroupPar (CParagraph 0 (10 #71# 0) 1 2 2 0 180) (CObjectList
            #431=(CLinePar (CParagraph 0 (1 #71# 1) 1 2 2 0 191) " N1: 3491 "
            1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG")
            #432=(CRSFolderPar 191) " N1: 1146 " 1))))) "NotI: 1 site") 2 6
            "GCGGCCGC" "CGCCGGCG") #423=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92073920 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3
            sites") 3 3 "TCGCGA" "AGCGCT") #424=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92074816 (8 0) 1 1 1 0 178) (CObjectList))
            "NsiI: 2 sites") 5 1 "ATGCAT" "TACGTA") #425=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92078624 (8 0) 1 1 1 0 178)
            (CObjectList #426=(CGroupPar (CParagraph 0 (10 #69# 0) 1 2 2 0 180)
            (CObjectList #427=(CLinePar (CGroupPar (CParagraph 92549352 (8 0) 1
            1 1 0 178) (CObjectList)) "HpyCH4IV: 23 sites") 1 3 "ACGT" "TGCA")
            #401=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92549392 (8
            0) 1 1 1 0 178) (CObjectList)) "HpyCH4V: 51 sites") 2 2 "TGCA"
            "ACGT") #402=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92058368 (8 0) 1 1 1 0 178) (CObjectList)) "KasI: 5 sites") 1 5
            "GGCGCC" "CCGCGG") #403=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92056608 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 2
            sites") 5 1 "GGTACC" "CCATGG") #404=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058752 (8 0) 1 1 1 0 178) (CObjectList))
            "HinP1I: 45 sites") 1 3 "GCGC" "CGCG") #397=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92055008 (8 0) 1 1 1 0 178)
            (CObjectList)) "HpaI: 2 sites") 3 3 "GTTAAC" "CAATTG")
            #398=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92055072 (8
            0) 1 1 1 0 178) (CObjectList)) "HpaII: 35 sites") 1 3 "CCGG"
            "GGCC") #399=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92055712 (8 0) 1 1 1 0 178) (CObjectList)) "HpyCH4I: 32 sites") 5 1
            "NCATGN" "NGTACN") #400=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083872 (8 0) 1 1 1 0 178) (CObjectList)) "MseI: 55
            sites") 1 3 "TTAA" "AATT") #409=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92058400 (8 0) 1 1 1 0 178) (CObjectList))
            "NaeI: 3 sites") 3 3 "GCCGGC" "CGGCCG") #410=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92086784 (8 0) 1 1 1 0 178)
            (CObjectList)) "NarI: 5 sites") 2 4 "GGCGCC" "CCGCGG")
            #411=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92063808 (8
            0) 1 1 1 0 178) (CObjectList)) "NcoI: 4 sites") 1 5 "CCATGG"
            "GGTACC") #412=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92056800 (8 0) 1 1 1 0 178) (CObjectList)) "MboI: 50 sites") 1 5
            "NGATCN" "NCTAGN") #405=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92057888 (8 0) 1 1 1 0 178) (CObjectList)) "MfeI: 3
            sites") 1 5 "CAATTG" "GTTAAC") #406=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92057952 (8 0) 1 1 1 0 178) (CObjectList))
            "MluI: 0 sites") 1 5 "ACGCGT" "TGCGCA") #407=(CRSFolderPar
            (CFolderPar (CGroupPar (CParagraph 92083904 (8 0) 1 1 1 0 178)
            (CObjectList)) "MscI: 2 sites") 3 3 "TGGCCA" "ACCGGT")
            #408=(CRSFolderPar (CFolderPar #454=(CGroupPar (CParagraph 0 (10
            #89# 0) 1 2 2 0 180) (CObjectList #455=(CLinePar (CParagraph 0 (1
            #89# 1) 1 2 2 0 191) " N1: 3531 " 1))))) "SnaBI: 1 site") 3 3
            "TACGTA" "ATGCAT") #456=(CRSFolderPar (CFolderPar (CGroupPar
            (CParagraph 92083936 (8 0) 1 1 1 0 178) (CObjectList
            #457=(CGroupPar (CParagraph 0 (10 #90# 0) 1 2 2 0 180) (CObjectList
            #458=(CLinePar (CParagraph 0 (1 #90# 1) 1 2 2 0 191) " N1: 3534 "
            1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #459=(CRSFolderPar
            #449=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92084032 (8
            0) 1 1 1 0 178) (CObjectList)) "SfoI: 5 sites") 3 3 "GGCGCC"
            "CCGCGG") #450=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph
            92084000 (8 0) 1 1 1 0 178) (CObjectList #451=(CGroupPar
            (CParagraph 0 (10 #88# 0) 1 2 2 0 180) (CObjectList #452=(CLinePar
            (CParagraph 0 (1 #88# 1) 1 2 2 0 191) " N1: 3497 " 1))))) "SmaI: 1
            site") 3 3 "CCCGGG" "GGGCCC") #453=(CRSFolderPar (CFolderPar
            (CGroupPar (CParagraph 92083968 (8 0) 1 1 1 0 178) (CObjectList
            (CFolderPar (CGroupPar (CParagraph 92085920 (8 0) 1 1 1 0 178)
            (CObjectList)) "StuI: 3 sites") 3 3 "AGGCCT" "TCCGGA")
            #464=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 92085856 (8
            0) 1 1 1 0 178) (CObjectList #465=(CGroupPar (CParagraph 0 (10 #96#
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            (CWidget 0 (3 #120# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 3.01299 1.9 0.082322 1) #565=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 63286324 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "Sp6 primer" "@N" 1 -5.00421
            -15.681 0 -10 4.62896 1.11561 #564#) #566=(CScratch (CWidget 0 (3
            #121# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507) 10 1 2.83442
            1.9 0.082322 1) #567=(CLabel (CWideLine (CWidget 0 (3 #118# 0) 1 2
            0 0 #486# 99148984 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334
            0) 0.835356 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #561=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 1371 100) (LOGPEN 0 0 0) 1 (LOGFONT
            69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "truncHIV-1 3 LTR" "@N" 1 -5.56886 -20.908 0 -10 7.14325 1.11561
            #560#) #562=(CWideArrow (CWideLine (CWidget 0 (3 #119# 0) 1 2 0 0
            #486# 63308488 100) (LOGPEN 0 0 6723840) 1 (LOGBRUSH 0 10079334 0)
            0.670712 3.02753 3.13281 1 0.082322) 0.8 1.8 0) #563=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (CObjectList
            #601=(CLine (CWidget 0 (3 #136# 0) 1 2 0 0 #490# 1834 100) (LOGPEN
            0 12 13395507) 10 0.835356 0.0971369 0.173916) #602=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #482# 59796852 100) (LOGPEN 0 0 0) 1
            (LOGFONT 69 26 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.74233 0.666667 0
            "SV40 PA terminator" "@N" 1 -7.08378 20.908 0 -10 8.20891 1.11561
            #601#)) (CObjectList))) (CObjectList)) #603=(CGroupWidget (CWidget
            11 (8 0) 1 2 0 0 Nil -2119 100) (CObjectList #604=(CGroupWidget
            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1805 100) (CObjectList
            #605=(CScratch 0.082322) 0.8 1.8 0) #597=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #482# 0 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "pBR322 origin" "@N" 1
            -16.4307 1.74233 0 -10 5.97768 1.11561 #596#) #598=(CScratch
            (CWidget 0 (3 #129# 0) 1 2 0 0 #490# 0 100) (LOGPEN 0 12 13395507)
            10 1 1.29419 1.9 0.082322 1) #599=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #482# 59795284 100) (LOGPEN 0 0 0) 1 (LOGFONT 69 26 0 0 700 0 0 0 0
            3 2 1 34 "Arial") 1.74233 0.666667 0 "SV40 origin" "@N" 1 -15.7111
            6.96932 0 -10 4.86207 1.11561 #598#)) (CObjectList))
            #600=(CGroupWidget (CWidget 43 (7 43 0) 1 2 0 0 Nil -2643 100)
            (LOGPEN 0 0 153) 1 (LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 18
            "Georgia") 1.74233 0.555556 128 "{\\i Asc}I (3500)" "@N (@S)" 5
            15.2669 -15.681 0 -10 4.59566 0.932452 #608#)) (CObjectList))
            #610=(CGroupWidget.
FEATURES             Location/Qualifiers
     source          1..14904
                     /mol_type="other DNA"
                     /note="/vntifkey=""""98"""""
                     /organism="pWPI"
     misc_feature    309..314
                     /Recognition_pattern="T^CCGGA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    455..635
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    746..790
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_psi_pack"
     misc_feature    1144..1151
                     /Recognition_pattern="GC^GGCCGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    1145..1150
                     /Recognition_pattern="C^GGCCG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGGCCG"
                     /label="BseX3I, BstZI, EagI, EclXI, Eco52I"
                     /note="Geneious type: restriction site"
     CDS             1178..2035
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\2"
     misc_feature    1300..1533
                     /note="/vntifkey=""""21"""""
                     /label="RRE"
     misc_feature    1930..1936
                     /Recognition_pattern="GG^GWCCC"
                     /annotation_group="GGGWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    2025..2030
                     /Recognition_pattern="G^TCGAC"
                     /Methylation="CpG:some blocked"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     promoter        2127..3297
                     /note="/vntifkey=""""30"""""
                     /label="EF1a_promoter"
     misc_feature    2198..2203
                     /Recognition_pattern="A^CCGGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    2818..2825
                     /Recognition_pattern="GGCCGG^CC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGCCGGCC"
                     /label="FseI, RigI"
                     /note="Geneious type: restriction site"
     misc_feature    2866..2872
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    3309..3316
                     /Recognition_pattern="ATTT^AAAT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATTTAAAT"
                     /label="SwaI, SmiI"
                     /note="Geneious type: restriction site"
     misc_feature    3317..3324
                     /Recognition_pattern="TTAAT^TAA"
                     /Methylation="EcoKI:cut"
                     /annotation_group="TTAATTAA"
                     /label="PacI"
                     /note="Geneious type: restriction site"
     misc_feature    3339..3344
                     /Recognition_pattern="GTT^AAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTAAC"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     misc_feature    3349..3364
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    3480..3487
                     /Recognition_pattern="VC^TCGAGB"
                     /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    3480..3487
                     /Recognition_pattern="CC^TCGAGG"
                     /Methylation="CpG:-"
                     /annotation_group="CCTCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     misc_feature    3487..3494
                     /Recognition_pattern="GTTT^AAAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTTAAAC"
                     /label="PmeI, MssI"
                     /note="Geneious type: restriction site"
     polylinker      3494..3499
                     /created_by="pbeare"
                     /modified_by="pbeare"
                     /label="SmaI site"
     misc_feature    3494..3499
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    3494..3499
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     Kozak           3501..3508
                     /created_by="pbeare"
     CDS             3509..5926
                     /created_by="pbeare"
                     /label="ACE2"
     misc_feature    3707..3717
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5071..5084)
                     /Recognition_pattern="(7/12)GAANNNNNNNTTGG(11/6)"
                     /annotation_group="GAANNNNNNNTTGG"
                     /label="AjuI"
                     /note="Geneious type: restriction site"
     misc_feature    5333..5342
                     /Recognition_pattern="GACNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    5927..5941
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          <5927..>5941
                     /mol_type="other DNA"
                     /note="/vntifkey=""""98"""""
                     /organism="pWPI"
     misc_feature    <5927..>5941
                     /note="/vntifkey=""""21"""""
                     /label="MCS"
     source          <5942..>5951
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     Kozak           5943..5951
                     /created_by="pbeare"
                     /label="Kozak sequence"
     CDS             5952..7430
                     /created_by="pbeare"
                     /modified_by="pbeare"
                     /label="TMPRSS2"
     misc_feature    5968..5974
                     /Recognition_pattern="G^GTNACC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GGTNACC"
                     /label="BstEII, BstPI, Eco91I, EcoO65I, PspEI"
                     /note="Geneious type: restriction site"
     misc_feature    6220..6226
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    6316..6322
                     /Recognition_pattern="CC^TNAGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     misc_feature    6479..6491
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /Methylation="CpG:impaired"
                     /annotation_group="GAACNNNNNNTAC"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     misc_feature    6649..6660
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /Methylation="CpG:cut"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     misc_feature    6740..6745
                     /Recognition_pattern="C^CCGGG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CCCGGG"
                     /label="Cfr9I, TspMI, XmaI"
                     /note="Geneious type: restriction site"
     misc_feature    6740..6745
                     /Recognition_pattern="CCC^GGG"
                     /Methylation="CpG:blocked"
                     /annotation_group="CCCGGG"
                     /label="SmaI"
                     /note="Geneious type: restriction site"
     misc_feature    6771..6776
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACGTC"
                     /label="BtrI, AjiI, BmgBI"
                     /note="Geneious type: restriction site"
     misc_feature    6864..6875
                     /Recognition_pattern="CCANNNNN^NTGG"
                     /Methylation="Dcm:variable"
                     /annotation_group="CCANNNNNNTGG"
                     /label="BstXI"
                     /note="Geneious type: restriction site"
     misc_feature    7207..7212
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    7292..7297
                     /Recognition_pattern="TT^CGAA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TTCGAA"
                     /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV,
                     SfuI"
                     /note="Geneious type: restriction site"
     misc_feature    7442..7448
                     /Recognition_pattern="CG^GWCCG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGGWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     misc_feature    7447..7452
                     /Recognition_pattern="C^GTACG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGTACG"
                     /label="BsiWI, Pfl23II, PspLI"
                     /note="Geneious type: restriction site"
     misc_feature    7449..7454
                     /Recognition_pattern="TAC^GTA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TACGTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     misc_feature    7454..7459
                     /Recognition_pattern="A^CTAGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    7459..7464
                     /Recognition_pattern="TGG^CCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGGCCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    7469..7474
                     /Recognition_pattern="T^GATCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGATCA"
                     /label="BclI, FbaI, Ksp22I"
                     /note="Geneious type: restriction site"
     misc_feature    7497..8059
                     /note="/vntifkey=""""21"""""
                     /label="IRES"
     misc_feature    7637..7642
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    7815..7821
                     /Recognition_pattern="CACCTGC(4/8)"
                     /annotation_group="CACCTGC"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8026..8031)
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CACGTC"
                     /label="BtrI, AjiI, BmgBI"
                     /note="Geneious type: restriction site"
     CDS             8063..8662
                     /note="/vntifkey=""""4"""""
                     /label="Puro(R)"
     misc_feature    8066..8675
                     /Original_Bases="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG
                     AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCA
                     GCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGG
                     GACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCT
                     GACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGG
                     TGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTG
                     ATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGA
                     GGGCTAACTAGCTAGTCGAGCTC"
                     /label="GCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGC
                     TACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCT
                     AGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTG
                     CAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTAT
                     CGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAG
                     GTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGC
                     CGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAACT
                     AGCTAGTCGAGCTC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8679..8684
                     /Recognition_pattern="TT^CGAA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TTCGAA"
                     /label="AsuII, Bpu14I, Bsp119I, BspT104I, BstBI, NspV,
                     SfuI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(8705..8721)
                     /note="/vntifkey=""""21"""""
                     /label="pBluescriptKS_primer"
     misc_feature    8711..8716
                     /Recognition_pattern="G^TCGAC"
                     /Methylation="CpG:some blocked"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    8716..8723
                     /Recognition_pattern="VC^TCGAGB"
                     /Methylation="CpG:some impaired, EcoBI:cut, EcoKI:cut"
                     /annotation_group="VCTCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    8716..8723
                     /Recognition_pattern="CC^TCGAGG"
                     /Methylation="CpG:-"
                     /annotation_group="CCTCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     enhancer        8736..9328
                     /note="/vntifkey=""""9"""""
                     /label="WPRE"
     misc_feature    9394..9415
                     /note="/vntifkey=""""21"""""
                     /label="U3PPT"
     misc_feature    9394..9409
                     /note="/vntifkey=""""21"""""
                     /label="cPPT"
     misc_feature    9466..9499
                     /note="/vntifkey=""""21"""""
                     /label="loxP"
     misc_feature    9519..9699
                     /note="/vntifkey=""""21"""""
                     /label="truncHIV-1_3_LTR"
     misc_feature    9519..9699
                     /note="/vntifkey=""""21"""""
                     /label="HIV-1_5_LTR"
     misc_feature    9703..9708
                     /Recognition_pattern="T^CTAGA"
                     /Methylation="Dam:blocked"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(9725..9742)
                     /note="/vntifkey=""""21"""""
                     /label="Sp6_primer"
     misc_feature    9801..9806
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(9951..9962)
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /Methylation="Dam:some blocked, CpG:some blocked"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     misc_feature    9962..9967
                     /Recognition_pattern="G^CTAGC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     misc_feature    9962..9967
                     /Recognition_pattern="GCTAG^C"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GCTAGC"
                     /label="BmtI, BspOI"
                     /note="Geneious type: restriction site"
     gene            9980..10243
                     /gene="tet (300 - 563)"
                     /note="/vntifkey=""""60"""""
     misc_feature    10003..10010
                     /Recognition_pattern="CR^CCGGYG"
                     /Methylation="CpG:variable, EcoBI:some blocked, EcoKI:some
                     blocked"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(10032..10051)
                     /note="/vntifkey=""""21"""""
                     /label="pBRrevBam_primer"
     misc_feature    10088..10093
                     /Recognition_pattern="AGC^GCT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    10379..10384
                     /Recognition_pattern="CGTCTC(1/5)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGTCTC"
                     /label="Esp3I, BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    10384..10406
                     /note="/vntifkey=""""21"""""
                     /label="pGEX_3_primer"
     misc_feature    complement(10433..10438)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGTCTC"
                     /label="Esp3I, BsmBI"
                     /note="Geneious type: restriction site"
     promoter        10565..10593
                     /note="/vntifkey=""""30"""""
                     /label="AmpR_promoter"
     gene            10635..11495
                     /gene="Ampicillin"
                     /note="/vntifkey=""""60"""""
     CDS             10635..11495
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\3"
     misc_feature    complement(10895..10906)
                     /Recognition_pattern="(10/12)CGANNNNNNTGC(12/10)"
                     /Methylation="Dam:some blocked, CpG:some blocked"
                     /annotation_group="CGANNNNNNTGC"
                     /label="BcgI"
                     /note="Geneious type: restriction site"
     misc_feature    11050..11055
                     /Recognition_pattern="CGAT^CG"
                     /Methylation="Mixed Methylation"
                     /annotation_group="CGATCG"
                     /label="PvuI, Ple19I"
                     /note="Geneious type: restriction site"
     misc_feature    11197..11202
                     /Recognition_pattern="TGC^GCA"
                     /Methylation="Mixed Methylation"
                     /annotation_group="TGCGCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    11417..11427
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     rep_origin      11650..12269
                     /note="/vntifkey=""""33"""""
                     /label="pBR322_origin"
     misc_feature    complement(12495..12515)
                     /note="/vntifkey=""""21"""""
                     /label="pBABE_3_primer"
     misc_feature    complement(12501..12954)
                     /note="/vntifkey=""""21"""""
                     /label="SV40_enhancer"
     promoter        12513..12781
                     /note="/vntifkey=""""30"""""
                     /label="SV40_promoter"
     misc_feature    12560..12565
                     /Recognition_pattern="ATGCA^T"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     misc_feature    12632..12637
                     /Recognition_pattern="ATGCA^T"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ATGCAT"
                     /label="EcoT22I, Mph1103I, NsiI, Zsp2I"
                     /note="Geneious type: restriction site"
     rep_origin      12680..12757
                     /note="/vntifkey=""""33"""""
                     /label="SV40_origin"
     misc_feature    12742..12761
                     /note="/vntifkey=""""21"""""
                     /label="SV40pro_F_primer"
     misc_feature    12760..12772
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /Methylation="Dcm:some impaired, CpG:variable"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     misc_feature    12814..12819
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /label="AvrII, AspA2I, BlnI, XmaJI"
                     /note="Geneious type: restriction site"
     misc_feature    13304..13309
                     /Recognition_pattern="A^CCGGT"
                     /Methylation="Mixed Methylation"
                     /annotation_group="ACCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(13310..13320)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /Methylation="Dcm:cut, CpG:variable, EcoBI:blocked"
                     /annotation_group="ACNNNNGTAYC"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    13640..13649
                     /Recognition_pattern="GACNN^NNGTC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GACNNNNGTC"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    13896..13908
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Methylation="CpG:-"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    13962..13977
                     /note="/vntifkey=""""21"""""
                     /label="SV40_int"
     misc_feature    13983..14030
                     /note="/vntifkey=""""21"""""
                     /label="SV40_3_splice"
     CDS             14107..14580
                     /note="/vntifkey=""""4"""""
                     /label="ORF\frame\1"
     misc_feature    14158..14178
                     /note="/vntifkey=""""21"""""
                     /label="SV40\NLS"
     misc_feature    14164..14178
                     /note="/vntifkey=""""21"""""
                     /label="NLS"
     misc_feature    14600..14605
                     /Recognition_pattern="GTT^AAC"
                     /Methylation="Mixed Methylation"
                     /annotation_group="GTTAAC"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     terminator      14606..14737
                     /note="/vntifkey=""""43"""""
                     /label="SV40_PA_terminator"
     misc_feature    14694..14713
                     /note="/vntifkey=""""21"""""
                     /label="EBV_rev_primer"
ORIGIN      
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaacccggga gcgccaccat gtcaagctct tcctggctcc ttctcagcct
     3541 tgttgctgta actgctgctc agtccaccat tgaggaacag gccaagacat ttttggacaa
     3601 gtttaaccac gaagccgaag acctgttcta tcaaagttca cttgcttctt ggaattataa
     3661 caccaatatt actgaagaga atgtccaaaa catgaataat gctggggaca aatggtctgc
     3721 ctttttaaag gaacagtcca cacttgccca aatgtatcca ctacaagaaa ttcagaatct
     3781 cacagtcaag cttcagctgc aggctcttca gcaaaatggg tcttcagtgc tctcagaaga
     3841 caagagcaaa cggttgaaca caattctaaa tacaatgagc accatctaca gtactggaaa
     3901 agtttgtaac ccagataatc cacaagaatg cttattactt gaaccaggtt tgaatgaaat
     3961 aatggcaaac agtttagact acaatgagag gctctgggct tgggaaagct ggagatctga
     4021 ggtcggcaag cagctgaggc cattatatga agagtatgtg gtcttgaaaa atgagatggc
     4081 aagagcaaat cattatgagg actatgggga ttattggaga ggagactatg aagtaaatgg
     4141 ggtagatggc tatgactaca gccgcggcca gttgattgaa gatgtggaac atacctttga
     4201 agagattaaa ccattatatg aacatcttca tgcctatgtg agggcaaagt tgatgaatgc
     4261 ctatccttcc tatatcagtc caattggatg cctccctgct catttgcttg gtgatatgtg
     4321 gggtagattt tggacaaatc tgtactcttt gacagttccc tttggacaga aaccaaacat
     4381 agatgttact gatgcaatgg tggaccaggc ctgggatgca cagagaatat tcaaggaggc
     4441 cgagaagttc tttgtatctg ttggtcttcc taatatgact caaggattct gggaaaattc
     4501 catgctaacg gacccaggaa atgttcagaa agcagtctgc catcccacag cttgggacct
     4561 ggggaagggc gacttcagga tccttatgtg cacaaaggtg acaatggacg acttcctgac
     4621 agctcatcat gagatggggc atatccagta tgatatggca tatgctgcac aaccttttct
     4681 gctaagaaat ggagctaatg aaggattcca tgaagctgtt ggggaaatca tgtcactttc
     4741 tgcagccaca cctaagcatt taaaatccat tggtcttctg tcacccgatt ttcaagaaga
     4801 caatgaaaca gaaataaact tcctgctcaa acaagcactc acgattgttg ggactctgcc
     4861 atttacttac atgttagaga agtggaggtg gatggtcttt aaaggggaaa ttcccaaaga
     4921 ccagtggatg aaaaagtggt gggagatgaa gcgagagata gttggggtgg tggaacctgt
     4981 gccccatgat gaaacatact gtgaccccgc atctctgttc catgtttcta atgattactc
     5041 attcattcga tattacacaa ggacccttta ccaattccag tttcaagaag cactttgtca
     5101 agcagctaaa catgaaggcc ctctgcacaa atgtgacatc tcaaactcta cagaagctgg
     5161 acagaaactg ttcaatatgc tgaggcttgg aaaatcagaa ccctggaccc tagcattgga
     5221 aaatgttgta ggagcaaaga acatgaatgt aaggccactg ctcaactact ttgagccctt
     5281 atttacctgg ctgaaagacc agaacaagaa ttcttttgtg ggatggagta ccgactggag
     5341 tccatatgca gaccaaagca tcaaagtgag gataagccta aaatcagctc ttggagataa
     5401 agcatatgaa tggaacgaca atgaaatgta cctgttccga tcatctgttg catatgctat
     5461 gaggcagtac tttttaaaag taaaaaatca gatgattctt tttggggagg aggatgtgcg
     5521 agtggctaat ttgaaaccaa gaatctcctt taatttcttt gtcactgcac ctaaaaatgt
     5581 gtctgatatc attcctagaa ctgaagttga aaaggccatc aggatgtccc ggagccgtat
     5641 caatgatgct ttccgtctga atgacaacag cctagagttt ctggggatac agccaacact
     5701 tggacctcct aaccagcccc ctgtttccat atggctgatt gtttttggag ttgtgatggg
     5761 agtgatagtg gttggcattg tcatcctgat cttcactggg atcagagatc ggaagaagaa
     5821 aaataaagca agaagtggag aaaatcctta tgcctccatc gatattagca aaggagaaaa
     5881 taatccagga ttccaaaaca ctgatgatgt tcagacctcc ttttagcgcg cctgcaggat
     5941 cagcaggcac catggctttg aactcagggt caccaccagc tattggacct tactatgaaa
     6001 accatggata ccaaccggaa aacccctatc ccgcacagcc cactgtggtc cccactgtct
     6061 acgaggtgca tccggctcag tactacccgt cccccgtgcc ccagtacgcc ccgagggtcc
     6121 tgacgcaggc ttccaacccc gtcgtctgca cgcagcccaa atccccatcc gggacagtgt
     6181 gcacctcaaa gactaagaaa gcactgtgca tcaccttgac cctggggacc ttcctcgtgg
     6241 gagctgcgct ggccgctggc ctactctgga agttcatggg cagcaagtgc tccaactctg
     6301 ggatagagtg cgactcctca ggtacctgca tcaacccctc taactggtgt gatggcgtgt
     6361 cacactgccc cggcggggag gacgagaatc ggtgtgttcg cctctacgga ccaaacttca
     6421 tccttcaggt gtactcatct cagaggaagt cctggcaccc tgtgtgccaa gacgactgga
     6481 acgagaacta cgggcgggcg gcctgcaggg acatgggcta taagaataat ttttactcta
     6541 gccaaggaat agtggatgac agcggatcca ccagctttat gaaactgaac acaagtgccg
     6601 gcaatgtcga tatctataaa aaactgtacc acagtgatgc ctgttcttca aaagcagtgg
     6661 tttctttacg ctgtatagcc tgcggggtca acttgaactc aagccgccag agcaggatcg
     6721 tgggcggcga gagcgcgctc ccgggggcct ggccctggca ggtcagcctg cacgtccaga
     6781 acgtccacgt gtgcggaggc tccatcatca cccccgagtg gatcgtgaca gccgcccact
     6841 gcgtggaaaa acctcttaac aatccatggc attggacggc atttgcgggg attttgagac
     6901 aatctttcat gttctatgga gccggatacc aagtagaaaa agtgatttct catccaaatt
     6961 atgactccaa gaccaagaac aatgacattg cgctgatgaa gctgcagaag cctctgactt
     7021 tcaacgacct agtgaaacca gtgtgtctgc ccaacccagg catgatgctg cagccagaac
     7081 agctctgctg gatttccggg tggggggcca ccgaggagaa agggaagacc tcagaagtgc
     7141 tgaacgctgc caaggtgctt ctcattgaga cacagagatg caacagcaga tatgtctatg
     7201 acaacctgat cacaccagcc atgatctgtg ccggcttcct gcaggggaac gtcgattctt
     7261 gccagggtga cagtggaggg cctctggtca cttcgaagaa caatatctgg tggctgatag
     7321 gggatacaag ctggggttct ggctgtgcca aagcttacag accaggagtg tacgggaatg
     7381 tgatggtatt cacggactgg atttatcgac aaatgagggc agacggctag ggatccatat
     7441 gcggaccgta cgtactagtg gccacggctg atcatacggg ctgcaggaat tccgcccccc
     7501 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     7561 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     7621 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     7681 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     7741 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     7801 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     7861 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7921 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7981 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     8041 tgaaaaacac gatgataata ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     8101 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     8161 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     8221 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     8281 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8341 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     8401 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     8461 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     8521 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8581 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8641 gacccgcaag cccggtgcct gacgggcgcg tctggaactt cgaattcgat atcaagctta
     8701 tcgcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     8761 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8821 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8881 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8941 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9001 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9061 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9121 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     9181 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9241 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9301 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac
     9361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9421 aattcactcc caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc
     9481 atacattata cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     9541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     9721 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     9781 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     9841 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     9901 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     9961 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
    10021 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
    10081 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
    10141 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
    10201 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
    10261 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
    10321 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    10381 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
    10441 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10501 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10561 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10621 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10681 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10741 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10801 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10861 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10921 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10981 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    11041 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11101 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11161 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11221 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11281 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11341 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11401 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11461 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11521 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11581 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11641 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11701 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11761 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    11821 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11881 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11941 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12001 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
    12061 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12121 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12181 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12241 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12301 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12361 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12421 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12481 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    12541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    12601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    12661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    12721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    12781 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
    12841 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    12901 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    12961 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    13021 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
    13081 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    13141 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    13201 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    13261 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    13321 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    13381 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    13441 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    13501 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    13561 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    13621 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    13681 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    13741 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    13801 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    13861 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    13921 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    13981 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    14041 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    14101 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    14161 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    14221 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    14281 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    14341 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    14401 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    14461 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    14521 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    14581 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    14641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    14701 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    14761 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tagtggtttc
    14821 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    14881 aaatatgtac tacaaactta gtag
//
