LOCUS       NM_001198778            4172 bp    mRNA    linear   PRI 24-JUN-2018
DEFINITION  Homo sapiens cullin 2 (CUL2), transcript variant 1, mRNA.
ACCESSION   NM_001198778
VERSION     NM_001198778.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Westrich JA, Warren CJ, Klausner MJ, Guo K, Liu CW, Santiago ML and
            Pyeon D.
  TITLE     Human Papillomavirus 16 E7 Stabilizes APOBEC3A Protein by
            Inhibiting Cullin 2-Dependent Protein Degradation
  JOURNAL   J. Virol. 92 (7), e01318-17 (2018)
   PUBMED   29367246
  REMARK    GeneRIF: direct evidence that both A3A and HPV16 E7 interact with
            CUL2, suggesting that the E7-CUL2 complex formed during HPV
            infection may regulate A3A protein levels in the cell.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Cardote TAF, Gadd MS and Ciulli A.
  TITLE     Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase
            Complex
  JOURNAL   Structure 25 (6), 901-911 (2017)
   PUBMED   28591624
  REMARK    GeneRIF: The crystal structure of a pentameric CRL2(VHL) complex,
            composed of Cul2, Rbx1, Elongin B, Elongin C, and pVHL reveals
            hydrophobic contact residues critical for the Cul2 interactions.
REFERENCE   3  (bases 1 to 4172)
  AUTHORS   Xu J, Fang Y, Wang X, Wang F, Tian Q, Li Y, Xie X, Cheng X and Lu
            W.
  TITLE     CUL2 overexpression driven by CUL2/E2F1/miR-424 regulatory loop
            promotes HPV16 E7 induced cervical carcinogenesis
  JOURNAL   Oncotarget 7 (21), 31520-31533 (2016)
   PUBMED   27153550
  REMARK    GeneRIF: Results suggest that E7 recruited CUL2, driven by
            CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates
            HPV16-induced cervical carcinogenesis.
REFERENCE   4  (bases 1 to 4172)
  AUTHORS   Uchida T, Tamaki Y, Ayaki T, Shodai A, Kaji S, Morimura T, Banno Y,
            Nishitsuji K, Sakashita N, Maki T, Yamashita H, Ito H, Takahashi R
            and Urushitani M.
  TITLE     CUL2-mediated clearance of misfolded TDP-43 is paradoxically
            affected by VHL in oligodendrocytes in ALS
  JOURNAL   Sci Rep 6, 19118 (2016)
   PUBMED   26751167
  REMARK    GeneRIF: misfolded TDP-43 is cleared by VHL/CUL2 in a step-wise
            manner via fragmentation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4172)
  AUTHORS   Nguyen HC, Yang H, Fribourgh JL, Wolfe LS and Xiong Y.
  TITLE     Insights into Cullin-RING E3 ubiquitin ligase recruitment:
            structure of the VHL-EloBC-Cul2 complex
  JOURNAL   Structure 23 (3), 441-449 (2015)
   PUBMED   25661653
REFERENCE   6  (bases 1 to 4172)
  AUTHORS   Wada H, Yeh ET and Kamitani T.
  TITLE     Identification of NEDD8-conjugation site in human cullin-2
  JOURNAL   Biochem. Biophys. Res. Commun. 257 (1), 100-105 (1999)
   PUBMED   10092517
REFERENCE   7  (bases 1 to 4172)
  AUTHORS   Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW
            and Kaelin WG Jr.
  TITLE     Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau
            tumor suppressor protein requires binding to complexes containing
            elongins B/C and Cul2
  JOURNAL   Mol. Cell. Biol. 18 (2), 732-741 (1998)
   PUBMED   9447969
REFERENCE   8  (bases 1 to 4172)
  AUTHORS   Pause A, Lee S, Worrell RA, Chen DY, Burgess WH, Linehan WM and
            Klausner RD.
  TITLE     The von Hippel-Lindau tumor-suppressor gene product forms a stable
            complex with human CUL-2, a member of the Cdc53 family of proteins
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 94 (6), 2156-2161 (1997)
   PUBMED   9122164
REFERENCE   9  (bases 1 to 4172)
  AUTHORS   Kipreos ET, Lander LE, Wing JP, He WW and Hedgecock EM.
  TITLE     cul-1 is required for cell cycle exit in C. elegans and identifies
            a novel gene family
  JOURNAL   Cell 85 (6), 829-839 (1996)
   PUBMED   8681378
REFERENCE   10 (bases 1 to 4172)
  AUTHORS   Kibel A, Iliopoulos O, DeCaprio JA and Kaelin WG Jr.
  TITLE     Binding of the von Hippel-Lindau tumor suppressor protein to
            Elongin B and C
  JOURNAL   Science 269 (5229), 1444-1446 (1995)
   PUBMED   7660130
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC401811.1, BC009591.1, AL392046.11 and AI089201.1.
            [WARNING] On Jun 2, 2019 this sequence was replaced by
            NM_001198778.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK300491.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-156               DC401811.1         1-156
            157-2840            BC009591.1         206-2889
            2841-4139           AL392046.11        43632-44930         c
            4140-4172           AI089201.1         1-33                c
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p11.21"
     gene            1..4172
                     /gene="CUL2"
                     /note="cullin 2"
                     /db_xref="GeneID:8453"
                     /db_xref="HGNC:HGNC:2552"
                     /db_xref="MIM:603135"
     exon            1..139
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2399
                     /gene="CUL2"
                     /note="isoform a is encoded by transcript variant 1;
                     CUL-2; testis secretory sperm-binding protein Li 238E"
                     /codon_start=1
                     /product="cullin-2 isoform a"
                     /protein_id="NP_001185707.1"
                     /db_xref="CCDS:CCDS55709.1"
                     /db_xref="GeneID:8453"
                     /db_xref="HGNC:HGNC:2552"
                     /db_xref="MIM:603135"
                     /translation="MYRVTWSTFWLRFQHYTCTMSLKPRVVDFDETWNKLLTTIKAVV
                     MLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQV
                     LVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGE
                     LALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFP
                     LKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPS
                     SYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQEL
                     QNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALT
                     SVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVF
                     QKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNK
                     FNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQH
                     FSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQM
                     NEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQ
                     EMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKC
                     IEVLIDKQYIERSQASADEYSYVA"
     exon            140..280
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            281..383
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            384..478
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            479..584
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            585..667
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            668..764
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            765..875
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1038
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1163
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1271
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1331
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1460
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1547
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1700
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1778
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1845
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2048
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2049..2150
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2267
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..4159
                     /gene="CUL2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcacagtag ggagtaccag gaggagagga agcttgggtg ccatgttgca gttgagccca
       61 aactgaatgc tgtctgtaga aggaaacaac aaactttgta ctttatgtac agagtaacat
      121 ggtcaacttt ttggcttaga tttcaacact acacttgcac aatgtctttg aaaccaagag
      181 tagtagattt tgatgaaaca tggaacaaac ttttgacgac aataaaagcc gtggtcatgt
      241 tggaatacgt cgaaagagca acatggaatg accgtttctc agatatctat gctttatgtg
      301 tggcctatcc tgaacccctt ggagaaagac tttatacaga aactaagatt tttttggaaa
      361 atcatgttcg gcatttgcat aagagagttt tggagtcaga agaacaagta cttgttatgt
      421 atcataggta ctgggaagaa tacagcaagg gtgcagacta tatggactgc ttatataggt
      481 atctcaacac ccagtttatt aaaaagaata aattaacaga agcggacctt cagtatggct
      541 atggtggtgt agatatgaat gaaccactta tggaaatagg agagctagca ttggatatgt
      601 ggaggaaatt gatggttgaa ccacttcagg ccatccttat ccgaatgctg ctccgagaaa
      661 tcaaaaatga tcgtggtgga gaagacccaa accagaaagt aatccatggg gttattaact
      721 cctttgttca tgttgaacag tataagaaaa aattcccctt aaagttttat caggaaattt
      781 ttgagtctcc ctttctgact gaaacaggag agtattacaa acaagaagct tcaaatttat
      841 tacaagaatc aaactgctca cagtatatgg aaaaggttct aggtagatta aaagatgaag
      901 aaattcgatg tcgaaaatac ctacatccaa gttcatatac taaggtgatt catgaatgtc
      961 aacaacgaat ggtagcagac cacttacagt ttttacatgc agaatgtcat aatataattc
     1021 gacaagagaa aaaaaatgac atggcaaata tgtacgtctt actccgtgct gtgtccactg
     1081 gtttacctca tatgattcag gagctgcaaa accacatcca tgatgagggc cttcgagcaa
     1141 ccagcaacct tactcaggaa aacatgccaa cactatttgt ggagtcagtt ttggaagtgc
     1201 atggtaaatt tgttcagctt atcaacactg ttttgaatgg tgatcagcat tttatgagtg
     1261 cgttggataa ggcccttacg tcagttgtaa attacagaga acctaagtct gtttgcaaag
     1321 cacctgaact gcttgctaag tactgtgaca acttactgaa gaagtcagcg aaagggatga
     1381 cagagaatga agtggaagac aggctcacga gcttcatcac agtgttcaaa tacattgatg
     1441 acaaggacgt ctttcaaaag ttctacgcaa gaatgctggc aaaacgttta attcatgggt
     1501 tatccatgtc tatggactct gaagaagcca tgatcaacaa attaaagcaa gcctgtggtt
     1561 atgagtttac cagcaagcta catcggatgt atacagatat gagtgtcagc gctgatctca
     1621 acaataagtt caacaatttt atcaaaaacc aagacacagt aatagatttg ggaattagtt
     1681 ttcaaatata tgttctacag gctggtgcgt ggcctcttac tcaggctcct tcatctacgt
     1741 ttgcaattcc ccaggaatta gaaaaaagtg tacagatgtt tgaattattt tatagccaac
     1801 atttcagtgg aaggaaactt acatggttac attatctgtg tacaggtgaa gttaaaatga
     1861 actatttggg caaaccatat gtagccatgg ttacaacata ccaaatggca gttcttcttg
     1921 cctttaacaa cagtgaaact gtcagttata aagagcttca ggacagcact cagatgaatg
     1981 aaaaggaact gacaaaaaca atcaaatcat tacttgatgt gaaaatgatt aaccatgatt
     2041 cagaaaagga agatattgat gcagaatctt cgttttcatt aaatatgaac tttagcagta
     2101 aaagaacaaa atttaaaatt actacatcaa tgcagaaaga cacaccacaa gaaatggagc
     2161 agactagaag tgcagttgat gaggaccgga aaatgtatct ccaagctgct atagttcgta
     2221 tcatgaaagc acgaaaagtg cttcggcaca atgcccttat tcaagaggtg attagccagt
     2281 caagagctag gtttaatccc agtatcagca tgattaagaa gtgtattgaa gttctgatag
     2341 acaaacaata catagaacgc agccaggcgt cggcagatga atacagctac gtcgcgtgat
     2401 gtcgctctcc tccagcgtgg tgtgagaaga tcattgccat caccatttgg tgtgttcctg
     2461 tgggaaaaag caggactgtg cctccataat ttggtcattt ggcagcccct gttttctgct
     2521 gtttacaaca tcaccagtgc cacgtcatga gcgtcaaaga aaatgcctag agatatttca
     2581 agctcatgtc attatgacat ttcttaaaac tttattaaaa gaatgagtga agtattgctg
     2641 aaaagtggaa attcggttgg gtaccatgct ttttctcccc ttcacgtttg cagttgatgt
     2701 gtcttttttt ttttttttaa tgtatcttaa aggacataaa atttaaaaac ttaaatattg
     2761 taatatgaca gataacctaa taattgtatc tacattaaaa tgacaaacat gatactgctg
     2821 cttgtcaaat aaaaaaaaaa taaagaaata gaatgccttt tttatgtgga tggagtatca
     2881 ggttgaccac aaaatatatt gactcaaagc agctaatgca tctttagttg cgtttttatc
     2941 tgaatggttt aattcacttg tactcctatt taaatcctac atgaaaaatg tctagattat
     3001 tgttcttgac tgcataggac tgcattcagc ataaagaatg ctttattttt atggattaga
     3061 tatattggat ctaaacattt tgaatcttga agatgtaatt ccatcagcag tttctggtgg
     3121 tgtgctactc cacagacatc gcagagtgtg agcaggatgc ttggtgacct caagtctggc
     3181 acagagagag cttttcattc aaaagttgtc tttcttcggt tgcataatcc attaattcta
     3241 gcatagacta gtaccctagc tctgtggcct tccctgagtc ttaggaaatc tatgatacca
     3301 acatattcct tctatatgcc tcccctacct gttaccctta caaccctcct ccaacagttt
     3361 agatactaga gtcactctca tcaatcacag atgtgcttag caatgcataa cctaaatact
     3421 tttttaaaaa agaaaattgt acattgtact gggtgccaca tatataaatc ccattatttt
     3481 gtttatttta tatatatata tatatataat atatatatat atatatctca acagcagtgt
     3541 taagagtact gcgatctatt atcatattta ttgtctatcc acaccatcac caccaccacc
     3601 acacccctcc tccctcaaca tacaattttt ctttatttta aaaaaaataa gagacggggt
     3661 ttcgccatgt ttcccaggct agtctggaac ttctggcctc aagcaatcct atctctgtct
     3721 cccaaagtgc tgggattaca agcatgagcc actgcatcca tccaacacaa aatttttaaa
     3781 atcggaatat tttaaagcaa atcacacaaa ttatttcact tataatactt cagtaaggcc
     3841 tttaaaaaat ccacagtgat attattactc ctaacaaaaa caataattac ttagtatcat
     3901 ctaatatgtg gttcatattt aaatttgttg ttttgagatg ggtcttacaa ttggtttatt
     3961 caattgcatt ttttctaact cgtgtctcaa gtgttttaaa aatctactga acttataatg
     4021 acttatataa tgtatttctc attttacctt tcttccaaaa gaggaaataa tggcaaacca
     4081 tataatattg tacattcact gtcaaaaagc aaacccttgt tttgataact tgttgattga
     4141 taaaagtttt ccaaattgaa aaaaaaaaaa aa
//

