LOCUS       NM_001008895            3909 bp    mRNA    linear   PRI 31-JUL-2017
DEFINITION  Homo sapiens cullin 4A (CUL4A), transcript variant 1, mRNA.
ACCESSION   NM_001008895
VERSION     NM_001008895.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Yang Y, Wang S, Li J, Qi S and Zhang D.
  TITLE     CUL4A as a marker and potential therapeutic target in multiple
            myeloma
  JOURNAL   Tumour Biol. 39 (7), 1010428317703923 (2017)
   PUBMED   28677427
  REMARK    GeneRIF: knockdown of cullin 4A via small interfering RNA inhibited
            the proliferation of the multiple myeloma cell lines by delaying
            cell-cycle progression and increasing apoptosis. cullin 4A
            downregulation inhibited multiple myeloma cell migration and
            invasion in vitro. Our results suggested that cullin 4A could be a
            promising therapy target in multiple myeloma patients
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Jia L, Yan F, Cao W, Chen Z, Zheng H, Li H, Pan Y, Narula N, Ren X,
            Li H and Zhou P.
  TITLE     Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer
  JOURNAL   J. Biol. Chem. 292 (7), 2966-2978 (2017)
   PUBMED   27974468
  REMARK    GeneRIF: findings revealed that CUL4A and CUL4B are differentially
            associated with etiologic factors for pulmonary malignancies and
            are independent prognostic markers for the survival of distinct
            lung cancer subtypes
REFERENCE   3  (bases 1 to 3909)
  AUTHORS   Englinger B, Mair M, Miklos W, Pirker C, Mohr T, van Schoonhoven S,
            Lotsch D, Korner W, Ferk F, Knasmuller S, Heffeter P, Keppler BK,
            Grusch M and Berger W.
  TITLE     Loss of CUL4A expression is underlying cisplatin hypersensitivity
            in colorectal carcinoma cells with acquired trabectedin resistance
  JOURNAL   Br. J. Cancer 116 (4), 489-500 (2017)
   PUBMED   28095394
  REMARK    GeneRIF: CUL4A sensitizes colorectal cancer cells to cisplatin
            induced cell death.
REFERENCE   4  (bases 1 to 3909)
  AUTHORS   Han X, Fang Z, Wang H, Jiao R, Zhou J and Fang N.
  TITLE     CUL4A functions as an oncogene in ovarian cancer and is directly
            regulated by miR-494
  JOURNAL   Biochem. Biophys. Res. Commun. 480 (4), 675-681 (2016)
   PUBMED   27983981
  REMARK    GeneRIF: Overexpression of miR-494 inhibited proliferation,
            migration, invasion and EMT of ovarian cancer cells by directly
            suppressing CUL4A expression. Therefore, our findings indicate that
            miR-494/CUL4A axis is important in the control of ovarian cancer
            tumorigenesis.
REFERENCE   5  (bases 1 to 3909)
  AUTHORS   Ren W, Sun Z, Zeng Q, Han S, Zhang Q and Jiang L.
  TITLE     Aberrant Expression of CUL4A Is Associated with IL-6/ STAT3
            Activation in Colorectal Cancer Progression
  JOURNAL   Arch. Med. Res. 47 (3), 214-222 (2016)
   PUBMED   27418574
  REMARK    GeneRIF: CUL4A played an oncogene role through ZEB1 in IL-6-induced
            colorectal carcinoma progression.
REFERENCE   6  (bases 1 to 3909)
  AUTHORS   Hori T, Osaka F, Chiba T, Miyamoto C, Okabayashi K, Shimbara N,
            Kato S and Tanaka K.
  TITLE     Covalent modification of all members of human cullin family
            proteins by NEDD8
  JOURNAL   Oncogene 18 (48), 6829-6834 (1999)
   PUBMED   10597293
REFERENCE   7  (bases 1 to 3909)
  AUTHORS   Ohta T, Michel JJ, Schottelius AJ and Xiong Y.
  TITLE     ROC1, a homolog of APC11, represents a family of cullin partners
            with an associated ubiquitin ligase activity
  JOURNAL   Mol. Cell 3 (4), 535-541 (1999)
   PUBMED   10230407
REFERENCE   8  (bases 1 to 3909)
  AUTHORS   Chen LC, Manjeshwar S, Lu Y, Moore D, Ljung BM, Kuo WL, Dairkee SH,
            Wernick M, Collins C and Smith HS.
  TITLE     The human homologue for the Caenorhabditis elegans cul-4 gene is
            amplified and overexpressed in primary breast cancers
  JOURNAL   Cancer Res. 58 (16), 3677-3683 (1998)
   PUBMED   9721878
REFERENCE   9  (bases 1 to 3909)
  AUTHORS   Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka
            K and Kato S.
  TITLE     A new NEDD8-ligating system for cullin-4A
  JOURNAL   Genes Dev. 12 (15), 2263-2268 (1998)
   PUBMED   9694792
REFERENCE   10 (bases 1 to 3909)
  AUTHORS   Kipreos ET, Lander LE, Wing JP, He WW and Hedgecock EM.
  TITLE     cul-1 is required for cell cycle exit in C. elegans and identifies
            a novel gene family
  JOURNAL   Cell 85 (6), 829-839 (1996)
   PUBMED   8681378
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB455959.1, BC008308.1, AL136221.38 and BM835927.1.
            [WARNING] On Aug 30, 2017 this sequence was replaced by
            NM_001008895.3.
            On Jun 13, 2013 this sequence version replaced NM_001008895.1.
            
            Summary: CUL4A is the ubiquitin ligase component of a multimeric
            complex involved in the degradation of DNA damage-response proteins
            (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.245263.1,
                                           SRR1660807.50789.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               DB455959.1         1-112
            113-3364            BC008308.1         1-3252
            3365-3853           AL136221.38        56354-56842
            3854-3909           BM835927.1         138-193
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..3909
                     /gene="CUL4A"
                     /note="cullin 4A"
                     /db_xref="GeneID:8451"
                     /db_xref="HGNC:HGNC:2554"
                     /db_xref="MIM:603137"
     CDS             124..2403
                     /gene="CUL4A"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CUL-4A"
                     /codon_start=1
                     /product="cullin-4A isoform 1"
                     /protein_id="NP_001008895.1"
                     /db_xref="CCDS:CCDS41908.1"
                     /db_xref="GeneID:8451"
                     /db_xref="HGNC:HGNC:2554"
                     /db_xref="MIM:603137"
                     /translation="MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLV
                     IKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPML
                     YKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTY
                     VLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLL
                     GMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVIT
                     YLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQ
                     ALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKE
                     SFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFE
                     AFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHF
                     KQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRK
                     LQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSEL
                     RRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQV
                     STTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLI
                     DRDYMERDKDNPNQYHYVA"
     misc_feature    151..153
                     /gene="CUL4A"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000244|PubMed:18669648,
                     ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:23186163,
                     ECO:0000244|PubMed:24275569}; propagated from
                     UniProtKB/Swiss-Prot (Q13619.3); phosphorylation site"
ORIGIN      
        1 gcgggcgggg cggggcgggg cgcgcgagga ggacggggcg gaggccgcgc ggaccggggc
       61 gggcggagcg gagctcggcg ggcggcggcg ctcggggcgg ggcgcggcgg ttccggccca
      121 gccatggcgg acgaggcccc gcggaagggc agcttctcgg cgctcgtggg ccgcaccaac
      181 ggcctcacca agcccgcggc cctggccgcc gcgcccgcca agccgggggg cgcgggcggc
      241 tccaagaagc tggtcatcaa gaacttccga gacagacctc ggctgcccga caactacacg
      301 caggacacgt ggcggaagct gcacgaggcg gtgcgggccg tgcagagcag cacctccatc
      361 aggtacaacc tcgaggagct ctaccaggct gtggaaaatc tctgttctca caaagtctcc
      421 ccaatgctct acaagcaact gcgtcaggcc tgtgaagacc acgtccaggc acagatcctt
      481 ccgtttagag aagactcact agatagtgtt ttatttttaa agaagattaa cacgtgctgg
      541 caggaccact gcagacaaat gatcatgatc agaagcatct tcctgttctt ggaccgcacc
      601 tatgtgctgc agaactccac gctgccctcc atctgggata tgggattaga actgtttaga
      661 acccatatta ttagtgataa aatggttcag agtaaaacca ttgatggaat cctactgctg
      721 atcgagcgcg agaggagcgg cgaggccgtg gaccggagcc tgttgcggag cctcctgggc
      781 atgctgtctg acctgcaggt gtataaagat tcatttgaac tgaaattttt ggaagagact
      841 aattgcttat atgctgccga aggccaaagg ttaatgcagg aaagagaggt tccagaatat
      901 cttaaccatg taagtaaacg cttagaggaa gagggagaca gagtaatcac ttacttggac
      961 cacagcacac agaaaccact gattgcttgt gtggagaaac agctattagg agaacattta
     1021 acagcaattc tgcagaaagg gctcgaccac ttactggatg agaacagagt gccggacctc
     1081 gcacagatgt accagctgtt cagccgggtg aggggcgggc agcaggcgct gctgcagcac
     1141 tggagcgagt acatcaagac ttttggaaca gcgatcgtaa tcaatcctga gaaagacaaa
     1201 gacatggtcc aagacctgtt ggacttcaag gacaaggtgg accacgtgat cgaggtctgc
     1261 ttccagaaga atgagcggtt cgtcaacctg atgaaggagt cctttgagac gttcatcaac
     1321 aagagaccca acaagcctgc agaactgatc gcaaagcatg tggattcaaa gttaagagca
     1381 ggcaacaaag aagccacaga cgaggagctg gagcggacgt tggacaagat catgatcctg
     1441 ttcaggttta tccacggtaa agatgtcttt gaagcatttt ataaaaaaga tttggcaaaa
     1501 agactccttg ttgggaaaag tgcctcagtc gatgctgaaa agtctatgtt gtcaaagctc
     1561 aagcatgagt gcggtgcagc cttcaccagc aagctggaag gcatgttcaa ggacatggag
     1621 ctttcgaagg acatcatggt tcatttcaag cagcatatgc agaatcagag tgactcaggc
     1681 cctatagacc tcacagtgaa catactcaca atgggctact ggccaacata cacgcccatg
     1741 gaagtgcact taaccccaga aatgattaaa cttcaggaag tatttaaggc attttatctt
     1801 ggaaagcaca gtggtcgaaa acttcagtgg caaactactt tgggacatgc tgttttaaaa
     1861 gcggagttta aagaagggaa gaaggaattc caggtgtccc tcttccagac actggtgctc
     1921 ctcatgttca acgagggaga tggcttcagc tttgaggaga taaaaatggc cacggggata
     1981 gaggatagtg aattgcgcag aacgctgcag tccctggcct gtggcaaagc acgtgtgctg
     2041 attaaaagtc ccaaaggaaa ggaagtggaa gatggagaca agttcatttt taatggagag
     2101 ttcaagcaca agttgtttag aataaagatc aatcaaattc agatgaagga aactgttgag
     2161 gaacaggtta gcaccactga gagagtgttt caggatagac aatatcagat tgatgctgct
     2221 atcgtcagaa taatgaagat gagaaagact cttggtcata atcttctagt ttctgaatta
     2281 tataatcagc tgaaatttcc agtaaagcct ggagatttga aaaagagaat tgaatctctg
     2341 atagacagag actatatgga gagagacaaa gacaatccga atcagtacca ctacgtggcc
     2401 tgacgcatct gcagacggtt ccccttcatg aaacactaga atgtaccctc agagcaggaa
     2461 gcacacctgt gccatttctg ggactctgat tgatccagct gtggacattg gaaggcgaag
     2521 gaagggaggt ggctcctggg tcatctttca caaggctcaa gacttcaacc tgcagatgta
     2581 tctttttccc tccagttttt cctctagttc ttttaggcat ttaaattgtt tctgttactc
     2641 tgtgcaaaat aactttgaga ttggacaaga agatgttact aaagagaagt tcctttaaaa
     2701 ggtcttgttc ttgtgtcaaa aagctgcaag tttggtttgt tctcgtgtgt gatcatgagt
     2761 gcacaatgaa gaagacccta gatgctgcat tttttagctc tgaagattcc ttaggtatcc
     2821 ctgaagacag ctcgctcaga tgatcagcat ttagagtgaa aacaagggcc cttcatgggt
     2881 gaacattaga aagagccagg gttcaaagct ggcgaatgga tgacgcaccc tagccactgg
     2941 cccctctctg tttcatgtat ttccaaaagt tgtaaacttt gatggctgat ttttcgtaag
     3001 tcaggtttct aagtgagctc cctgaggtgc caaggccatg gtgtccgccc tgctgcgtct
     3061 gttcgtcagc tgagttcctt gtgaatctct gttttagggt ttggggctag tgtgtttgtg
     3121 tttccattct aagattgagt ctggcagtcc ctgttttttt gcattggggt aactgctctt
     3181 tgattttttt taattgcagt atttgtgtga ttgcaataat aaagtttggt ttggttttta
     3241 cagtcatgcg cagggacgat ccttgttctc tgctgtaaac tgtaaaaagt ttatggagac
     3301 ttaaagtctt gatgttgtga agcagaggtt attttgtgga aagattaaaa ggattttgtt
     3361 ggtacctggt tttgtgttgt gtatatatac atgaggttga acagtgaaag gaaagttcag
     3421 tagtgatgtt agaagggtaa ctatgacaaa gatacttttg agataacatt taaaagtact
     3481 ttatatttta cataatagca tgtttcattt tgattaaaag ctaccaaagg aattttgatc
     3541 atggcataag tgtttaaagc aatattttct ggaatatacc aagtttatat aatttgattt
     3601 tgtgctaaat tattaagagt ctctttttga aacatgcggg tttgaaatat gacaccttgt
     3661 gggtttccat attaaaatcc tcactcttta attgtcattt ctatctttga aaattttcat
     3721 ttatgagttc catgatatgt ggtctaagaa agaccaaaca gatttctatt ttttttttct
     3781 tataagttcg ttgtgtctag agattgttaa tattgtaatt taatgtagac ttactttgaa
     3841 taaaattagt ttaattggcc ttaaaattac attaataaaa ctttgtgata tgcaaatgaa
     3901 aaaaaaaaa
//

